FvH4_6g29040

Brca1-associated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0013575
Physical Location & Seq
Reverse (-)
22406604 .. 22410790
4187 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g29040.t1

Sequence Viewer

Length: 1539 bp
ATGGGAATAGAAAGACGATCCTTTCGCAGAAACCCAAAGGCGCAAAACTCTCCCCGAAACTCAAAACCCGTCAACATGTCGCCGAGCTCGAGCACAGCGCGCACCGGAGCCTCGCCGATCGAGGCCTTCAGGTCGCCGCCTTCTATCATTCCCGGCGAGCCCATCGACTTCGCATATTCCTCTTCTTCATCATCCATCTCAAACATCACGCAGGCTTTTCCTTTCTCTTCTGGAAACCCTAGAATCGAAGAGACCCGAGGCATCATGCTCCTCTACCGCGACGACGACGTCGTCTCCTTCTCTCCCTCCGATCTTCCTGTTGAGAGGAAGCCGCTTGTTTGCGTACTCGGAGTGCCAAATCACATGACATATGCCGACTTCTGTAGGTTCTGTGGGTCTTTCATTCAGCATATATTGGAGATGCGGATTGTCAGGACCGATGGAATGGAGGATCGTTATAGCGTTTTGATTAGGTTTGATAGTCAGGACTCCACAGATCATTTTTACAAGCACTTCAATCGAAGCCGGTTCTCATCACTAGAGGTGGAGGTCTGTCGGATACTTTTCACGGTTGATGTTCAATACACTGGTTCAATAGAACATGCACAGGCTCCATGTAGCTCAACTGAGCAGCCGTCGTGTCCAGTCTGTCTTGAGAGACTAGACCAAGATATGAGTGGAATTCTCACAACTATCTGCAACCATTCTTTCCATTGCTCTTGCATTTCAAAATGGACTGATACTTCTTGTCCTGTTTGTAGATATTGTCAGCAGCAGCCTGAAAAGTCAAATTGTTTCATTTGTCAAACTTCTGAGAATCTCTGGATGTGTATTATCTGTGGCTTTGTTGGTTGTGGAAGGTATAAAGAAGGCCATGCCATAAAACATTGGAAAGAAACACAACATTGCTATTCCCTTGAAGTGGAAACACAGCGTGTGTGGGATTATGCAGGAGACAATTATGTTCACCGCCTAATTCAATCAAAAACTGATGGAAAATTGGTTGAATTGAATTCACACTGTCGGAATGCGATTGGTTTCAAAGGAAATTGCGAGTGTGTAGATTCTGGATTCAGTGAGGCTCTTTTGAACAGTAGAGTAGAAGCTATTGTTAATGAGTACAACGAATTGCTTGCTACTCAACTTGAGAACCAGAAAATGTATTTTGAATCATTACTTCAAGATGTTAAAGAAGGAACTGAGAGAGAAATTTCTAAAGCTGTAGAAAAGGCTGTGCCAGTAAAGAAAATGCAGGACAAGCTGGATAGATGTGTAAAGGAGAAGAAATTTCTGGATGATCTTAATGATAATCTTTTGAAAAATCGAGACGTTTGGAAAGCAAAGATACTTGAAGTTGAAGAGAGGGAGAGAAAGGCTTCAAAACTGAAGGAAGATAAAATAAGCAACTTAGAAGAGCAGCTTAGGAATTTGATGCTGTACCTCGAGACAGGGGATACAGTAGAGTCTTCAAATGAAACAAATGATGGTACGGTCTTGCCAGAATCAATCAACTCGTCTCAGCCATCAATTATCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
BRAP2 zf-RING_2 zf-UBP

Protein Analysis

513

Amino Acids

58.3

Weight (kDa)

5.76

Isoelectric Point (pI)

51.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BRAP2 PF07576 106 - 202 1.7e-38 BRCA1-associated protein 2
zf-rbx1 PF12678 206 - 254 1.4e-06 RING-H2 zinc finger domain
zf-RING_2 PF13639 213 - 254 2e-08 Ring finger domain
zf-UBP PF02148 265 - 324 8.2e-23 Zn-finger in ubiquitin-hydrolases and other protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013575)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26000 AT2G26000 AT2G26000
fragaria_vesca FvH4_6g29040
malus_domestica MD17G1218900.v1.1
prunus_persica Prupe.3G094400_v2.0.a1 Prupe.3G094400_v2.0.a1
pyrus_communis pycom17g22370
rosa_chinensis RchiOBHm_Chr2g0133711
rosa_laevigata RLG00000019358
rosa_multiflora Rmu_sc0013154.1_g000008
rosa_roxburghii Rroxscaffold_2G00110880
rosa_rugosa Rorug02G0313200
rosa_samantha Rh2AG366600 Rh2BG372300 Rh2CG349900 Rh2DG389200
rosa_wichuraiana Rw2G029830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 287, 290
AatII GACGTC 1 cut(s) 291
AccII CGCG 2 cut(s) 100, 279
AciI CCGC 5 cut(s) 137, 277, 332, 424, 970
AclWI GGATC 2 cut(s) 12, 459
AcsI RAATTY 5 cut(s) 681, 1012, 1209, 1286, 1426
AcuI CTGAAG 2 cut(s) 112, 1406
AcyI GRCGYC 1 cut(s) 288
AdeI CACNNNGTG 1 cut(s) 935
AfaI GTAC 4 cut(s) 345, 1121, 1439, 1489
AfiI CCNNNNNNNGG 1 cut(s) 922
AflIII ACRYGT 1 cut(s) 75
AluBI AGCT 6 cut(s) 87, 621, 1106, 1220, 1261, 1420
AluI AGCT 6 cut(s) 87, 621, 1106, 1220, 1261, 1420
Alw21I GWGCWC 2 cut(s) 89, 95
Alw26I GTCTC 7 cut(s) 245, 298, 652, 948, 1320, 1439, 1521
AlwI GGATC 2 cut(s) 12, 459
Ama87I CYCGRG 3 cut(s) 88, 255, 1442
AoxI GGCC 2 cut(s) 123, 871
ApeKI GCWGC 4 cut(s) 631, 772, 775, 1417
ApoI RAATTY 5 cut(s) 681, 1012, 1209, 1286, 1426
ArsI GACNNNNNNTTYG 1 cut(s) 38
Asp700I GAANNNNTTC 1 cut(s) 1375
AspLEI GCGC 3 cut(s) 43, 100, 102
AspS9I GGNCC 1 cut(s) 435
AsuC2I CCSGG 1 cut(s) 153
AsuHPI GGTGA 1 cut(s) 959
AvaI CYCGRG 3 cut(s) 88, 255, 1442
AvaII GGWCC 1 cut(s) 435
BanII GRGCYC 2 cut(s) 89, 162
BbsI GAAGAC 1 cut(s) 1458
Bbv12I GWGCWC 2 cut(s) 89, 95
BbvI GCAGC 4 cut(s) 643, 784, 787, 1429
BccI CCATC 6 cut(s) 170, 203, 434, 986, 1478, 1531
BceAI ACGGC 1 cut(s) 619
BcgI CGANNNNNNTGC 4 cut(s) 500, 534, 1115, 1149
BciVI GTATCC 2 cut(s) 552, 1447
BcnI CCSGG 1 cut(s) 153
BcoDI GTCTC 7 cut(s) 245, 298, 652, 948, 1320, 1439, 1521
BfaI CTAG 3 cut(s) 240, 539, 662
BfmI CTRYAG 2 cut(s) 382, 1221
BfuI GTATCC 2 cut(s) 552, 1447
BisI GCNGC 6 cut(s) 137, 332, 632, 773, 776, 1418
BlsI GCNGC 6 cut(s) 138, 333, 633, 774, 777, 1419
Bme1390I CCNGG 1 cut(s) 153
Bme18I GGWCC 1 cut(s) 435
BmeT110I CYCGRG 3 cut(s) 88, 255, 1442
BmgT120I GGNCC 1 cut(s) 435
BmiI GGNNCC 2 cut(s) 109, 612
BmrFI CCNGG 1 cut(s) 153
BmsI GCATC 3 cut(s) 270, 411, 1422
BpiI GAAGAC 1 cut(s) 1458
Bpu10I CCTNAGC 1 cut(s) 1421
BpuEI CTTGAG 2 cut(s) 674, 1166
BpuMI CCSGG 1 cut(s) 153
BsaHI GRCGYC 1 cut(s) 288
BsaI GGTCTC 1 cut(s) 245
BsaJI CCNNGG 1 cut(s) 256
BsaWI WCCGGW 1 cut(s) 104
BsaXI ACNNNNNCTCC 2 cut(s) 278, 308
Bsc4I CCNNNNNNNGG 1 cut(s) 922
Bse118I RCCGGY 1 cut(s) 525
Bse1I ACTGG 3 cut(s) 592, 644, 1238
Bse3DI GCAATG 2 cut(s) 712, 904
BseDI CCNNGG 1 cut(s) 256
BseGI GGATG 3 cut(s) 191, 831, 1300
BseLI CCNNNNNNNGG 1 cut(s) 922
BseMI GCAATG 2 cut(s) 712, 904
BseMII CTCAG 4 cut(s) 618, 804, 1191, 1532
BseNI ACTGG 3 cut(s) 592, 644, 1238
BsePI GCGCGC 1 cut(s) 98
BseRI GAGGAG 1 cut(s) 260
BseXI GCAGC 4 cut(s) 643, 784, 787, 1429
Bsh1236I CGCG 2 cut(s) 100, 279
Bsh1285I CGRYCG 1 cut(s) 120
BshFI GGCC 2 cut(s) 125, 873
BsiEI CGRYCG 1 cut(s) 120
BsiHKAI GWGCWC 2 cut(s) 89, 95
BsiHKCI CYCGRG 3 cut(s) 88, 255, 1442
BsiSI CCGG 3 cut(s) 105, 153, 526
BslI CCNNNNNNNGG 1 cut(s) 922
BsmAI GTCTC 7 cut(s) 245, 298, 652, 948, 1320, 1439, 1521
BsmBI CGTCTC 3 cut(s) 298, 1320, 1521
BsmI GAATGC 1 cut(s) 1033
BsnI GGCC 2 cut(s) 125, 873
Bso31I GGTCTC 1 cut(s) 245
BsoBI CYCGRG 3 cut(s) 88, 255, 1442
Bsp1286I GDGCHC 3 cut(s) 89, 95, 162
Bsp143I GATC 6 cut(s) 17, 117, 310, 451, 496, 1297
BspACI CCGC 5 cut(s) 137, 277, 332, 424, 970
BspANI GGCC 2 cut(s) 125, 873
BspCNI CTCAG 4 cut(s) 619, 805, 1192, 1531
BspFNI CGCG 2 cut(s) 100, 279
BspLI GGNNCC 2 cut(s) 109, 612
BspPI GGATC 2 cut(s) 12, 459
BspQI GCTCTTC 1 cut(s) 1407
BspTNI GGTCTC 1 cut(s) 245
BsrDI GCAATG 2 cut(s) 712, 904
BsrFI RCCGGY 1 cut(s) 525
BsrI ACTGG 3 cut(s) 592, 644, 1238
BssAI RCCGGY 1 cut(s) 525
BssECI CCNNGG 1 cut(s) 256
BssHII GCGCGC 1 cut(s) 98
BssMI GATC 6 cut(s) 17, 117, 310, 451, 496, 1297
BssNI GRCGYC 1 cut(s) 288
Bst4CI ACNGT 5 cut(s) 571, 1022, 1094, 1459, 1492
Bst6I CTCTTC 5 cut(s) 187, 232, 243, 1353, 1407
BstACI GRCGYC 1 cut(s) 288
BstC8I GCNNGC 4 cut(s) 100, 158, 213, 1134
BstDEI CTNAG 6 cut(s) 627, 813, 1200, 1408, 1421, 1518
BstF5I GGATG 3 cut(s) 191, 831, 1300
BstFNI CGCG 2 cut(s) 100, 279
BstHHI GCGC 3 cut(s) 43, 100, 102
BstKTI GATC 6 cut(s) 20, 120, 313, 454, 499, 1300
BstMAI GTCTC 7 cut(s) 245, 298, 652, 948, 1320, 1439, 1521
BstMBI GATC 6 cut(s) 17, 117, 310, 451, 496, 1297
BstMCI CGRYCG 1 cut(s) 120
BstMWI GCNNNNNNNGC 2 cut(s) 99, 1258
BstNSI RCATGY 2 cut(s) 79, 605
BstSCI CCNGG 1 cut(s) 151
BstSFI CTRYAG 2 cut(s) 382, 1221
BstUI CGCG 2 cut(s) 100, 279
BstV1I GCAGC 4 cut(s) 643, 784, 787, 1429
BstV2I GAAGAC 1 cut(s) 1458
BsuI GTATCC 2 cut(s) 552, 1447
BsuRI GGCC 2 cut(s) 125, 873
BtsCI GGATG 3 cut(s) 191, 831, 1300
BtsIMutI CAGTG 3 cut(s) 585, 1018, 1081
Cac8I GCNNGC 4 cut(s) 100, 158, 213, 1134
CfoI GCGC 3 cut(s) 43, 100, 102
Cfr10I RCCGGY 1 cut(s) 525
Cfr13I GGNCC 1 cut(s) 435
Csp6I GTAC 4 cut(s) 344, 1120, 1438, 1488
CviAII CATG 6 cut(s) 76, 265, 364, 602, 615, 875
CviQI GTAC 4 cut(s) 344, 1120, 1438, 1488
DdeI CTNAG 6 cut(s) 627, 813, 1200, 1408, 1421, 1518
DpnI GATC 6 cut(s) 19, 119, 312, 453, 498, 1299
DpnII GATC 6 cut(s) 17, 117, 310, 451, 496, 1297
DraIII CACNNNGTG 1 cut(s) 935
DrdI GACNNNNNNGTC 2 cut(s) 287, 290
DseDI GACNNNNNNGTC 2 cut(s) 287, 290
Eam1104I CTCTTC 5 cut(s) 187, 232, 243, 1353, 1407
EarI CTCTTC 5 cut(s) 187, 232, 243, 1353, 1407
Ecl136II GAGCTC 1 cut(s) 87
Eco147I AGGCCT 1 cut(s) 125
Eco24I GRGCYC 2 cut(s) 89, 162
Eco31I GGTCTC 1 cut(s) 245
Eco47I GGWCC 1 cut(s) 435
Eco53kI GAGCTC 1 cut(s) 87
Eco57I CTGAAG 2 cut(s) 112, 1406
Eco88I CYCGRG 3 cut(s) 88, 255, 1442
EcoICRI GAGCTC 1 cut(s) 87
EcoRI GAATTC 2 cut(s) 681, 1012
EcoT38I GRGCYC 2 cut(s) 89, 162
Esp3I CGTCTC 3 cut(s) 298, 1320, 1521
FaeI CATG 6 cut(s) 79, 268, 367, 605, 618, 878
FalI AAGNNNNNCTT 2 cut(s) 1404, 1436
FatI CATG 6 cut(s) 75, 264, 363, 601, 614, 874
FauNDI CATATG 1 cut(s) 370
Fnu4HI GCNGC 6 cut(s) 137, 332, 632, 773, 776, 1418
FokI GGATG 3 cut(s) 178, 838, 1307
FriOI GRGCYC 2 cut(s) 89, 162
Fsp4HI GCNGC 6 cut(s) 137, 332, 632, 773, 776, 1418
FspBI CTAG 3 cut(s) 240, 539, 662
GlaI GCGC 3 cut(s) 42, 99, 101
GluI GCNGC 6 cut(s) 137, 332, 632, 773, 776, 1418
HaeIII GGCC 2 cut(s) 125, 873
HapII CCGG 3 cut(s) 105, 153, 526
HhaI GCGC 3 cut(s) 43, 100, 102
Hin1I GRCGYC 1 cut(s) 288
Hin1II CATG 6 cut(s) 79, 268, 367, 605, 618, 878
Hin6I GCGC 3 cut(s) 41, 98, 100
HinP1I GCGC 3 cut(s) 41, 98, 100
HincII GTYRAC 1 cut(s) 73
HindII GTYRAC 1 cut(s) 73
HinfI GANTC 8 cut(s) 243, 488, 817, 1064, 1071, 1169, 1463, 1502
HpaII CCGG 3 cut(s) 105, 153, 526
HphI GGTGA 1 cut(s) 959
Hpy166II GTNNAC 2 cut(s) 73, 967
Hpy188I TCNGA 5 cut(s) 310, 350, 558, 814, 1026
Hpy8I GTNNAC 2 cut(s) 73, 967
Hpy99I CGWCG 5 cut(s) 284, 287, 290, 293, 640
HpyAV CCTTC 7 cut(s) 136, 150, 307, 852, 863, 1187, 1381
HpyCH4III ACNGT 5 cut(s) 571, 1022, 1094, 1459, 1492
HpyCH4IV ACGT 2 cut(s) 288, 1329
HpyCH4V TGCA 5 cut(s) 605, 699, 723, 950, 1252
HpyF10VI GCNNNNNNNGC 2 cut(s) 99, 1258
HpyF3I CTNAG 6 cut(s) 627, 813, 1200, 1408, 1421, 1518
HpySE526I ACGT 2 cut(s) 288, 1329
Hsp92I GRCGYC 1 cut(s) 288
Hsp92II CATG 6 cut(s) 79, 268, 367, 605, 618, 878
HspAI GCGC 3 cut(s) 41, 98, 100
Kzo9I GATC 6 cut(s) 17, 117, 310, 451, 496, 1297
LguI GCTCTTC 1 cut(s) 1407
LmnI GCTCC 3 cut(s) 107, 273, 616
Lsp1109I GCAGC 4 cut(s) 643, 784, 787, 1429
LweI GCATC 3 cut(s) 270, 411, 1422
MaeI CTAG 3 cut(s) 240, 539, 662
MaeII ACGT 2 cut(s) 288, 1329
MalI GATC 6 cut(s) 19, 119, 312, 453, 498, 1299
MboI GATC 6 cut(s) 17, 117, 310, 451, 496, 1297
MhlI GDGCHC 3 cut(s) 89, 95, 162
MlyI GAGTC 2 cut(s) 482, 1472
MmeI TCCRAC 2 cut(s) 536, 1004
MroXI GAANNNNTTC 1 cut(s) 1375
MseI TTAA 3 cut(s) 1113, 1188, 1302
MspI CCGG 3 cut(s) 105, 153, 526
MspR9I CCNGG 1 cut(s) 153
Mva1269I GAATGC 1 cut(s) 1033
MvnI CGCG 2 cut(s) 100, 279
MwoI GCNNNNNNNGC 2 cut(s) 99, 1258
NciI CCSGG 1 cut(s) 153
NdeI CATATG 1 cut(s) 370
NdeII GATC 6 cut(s) 17, 117, 310, 451, 496, 1297
NlaIII CATG 6 cut(s) 79, 268, 367, 605, 618, 878
NlaIV GGNNCC 2 cut(s) 109, 612
NmeAIII GCCGAG 1 cut(s) 108
NspI RCATGY 2 cut(s) 79, 605
PaeR7I CTCGAG 2 cut(s) 88, 1442
PauI GCGCGC 1 cut(s) 98
PceI AGGCCT 1 cut(s) 125
PciI ACATGT 1 cut(s) 75
PciSI GCTCTTC 1 cut(s) 1407
PcsI WCGNNNNNNNCGW 2 cut(s) 86, 288
PctI GAATGC 1 cut(s) 1033
PdmI GAANNNNTTC 1 cut(s) 1375
PfeI GAWTC 6 cut(s) 243, 817, 1064, 1071, 1169, 1502
PflFI GACNNNGTC 2 cut(s) 287, 290
PkrI GCNGC 6 cut(s) 138, 333, 633, 774, 777, 1419
Ple19I CGATCG 1 cut(s) 120
PleI GAGTC 2 cut(s) 482, 1471
PpsI GAGTC 2 cut(s) 482, 1471
PscI ACATGT 1 cut(s) 75
Psp124BI GAGCTC 1 cut(s) 89
PspN4I GGNNCC 2 cut(s) 109, 612
PspPI GGNCC 1 cut(s) 435
PspXI VCTCGAGB 1 cut(s) 88
PsyI GACNNNGTC 2 cut(s) 287, 290
PteI GCGCGC 1 cut(s) 98
PvuI CGATCG 1 cut(s) 120
RsaI GTAC 4 cut(s) 345, 1121, 1439, 1489
RsaNI GTAC 4 cut(s) 344, 1120, 1438, 1488
SacI GAGCTC 1 cut(s) 89
SapI GCTCTTC 1 cut(s) 1407
SaqAI TTAA 3 cut(s) 1113, 1188, 1302
SatI GCNGC 6 cut(s) 137, 332, 632, 773, 776, 1418
Sau3AI GATC 6 cut(s) 17, 117, 310, 451, 496, 1297
Sau96I GGNCC 1 cut(s) 435
SchI GAGTC 2 cut(s) 482, 1472
ScrFI CCNGG 1 cut(s) 153
SduI GDGCHC 3 cut(s) 89, 95, 162
SfaNI GCATC 3 cut(s) 270, 411, 1422
SfcI CTRYAG 2 cut(s) 382, 1221
Sfr274I CTCGAG 2 cut(s) 88, 1442
SinI GGWCC 1 cut(s) 435
SlaI CTCGAG 2 cut(s) 88, 1442
SmlI CTYRAG 4 cut(s) 88, 653, 1145, 1442
SmoI CTYRAG 4 cut(s) 88, 653, 1145, 1442
SseBI AGGCCT 1 cut(s) 125
SsiI CCGC 5 cut(s) 137, 277, 332, 424, 970
SspMI CTAG 3 cut(s) 240, 539, 662
SstI GAGCTC 1 cut(s) 89
StuI AGGCCT 1 cut(s) 125
StyD4I CCNGG 1 cut(s) 151
TaaI ACNGT 5 cut(s) 571, 1022, 1094, 1459, 1492
TaiI ACGT 2 cut(s) 291, 1332
TaqI TCGA 7 cut(s) 89, 120, 165, 246, 520, 1324, 1443
TaqII GACCGA 1 cut(s) 452
TatI WGTACW 1 cut(s) 1119
TauI GCSGC 2 cut(s) 139, 334
TfiI GAWTC 6 cut(s) 243, 817, 1064, 1071, 1169, 1502
Tru1I TTAA 3 cut(s) 1113, 1188, 1302
Tru9I TTAA 3 cut(s) 1113, 1188, 1302
TscAI CASTG 3 cut(s) 592, 1025, 1081
TseI GCWGC 4 cut(s) 631, 772, 775, 1417
TspDTI ATGAA 4 cut(s) 177, 391, 787, 1488
TspRI CASTG 3 cut(s) 592, 1025, 1081
Tth111I GACNNNGTC 2 cut(s) 287, 290
VpaK11BI GGWCC 1 cut(s) 435
XapI RAATTY 5 cut(s) 681, 1012, 1209, 1286, 1426
XceI RCATGY 2 cut(s) 79, 605
XcmI CCANNNNNNNNNTGG 1 cut(s) 674
XhoI CTCGAG 2 cut(s) 88, 1442
XmnI GAANNNNTTC 1 cut(s) 1375
XspI CTAG 3 cut(s) 240, 539, 662
ZraI GACGTC 1 cut(s) 289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.