Rh2CG349900

Brca1-associated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0013575
Physical Location & Seq
Reverse (-)
47242578 .. 47247026
4449 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG349900.1

Sequence Viewer

Length: 1047 bp
ATGTCGCCGAGCTCGAGCACAGCACGTGCCGGAGCCTCGCCGGTCGAGGCCTTCAGGTCGCCGCCTTCGATAACTCCCGGCGAGCCTAGCGACTTCTCCTGTTCCTCCTCTTCATCCTCCATCTCAAACATCACGCAGGCCTTTCCTTTCTCTTCTGGAAACCCTAGAATTGAAGAGACCAGAGGCATCATGCTCCTCTACCGCGACGATGACGTCGTCTCGTTCTCTCCGTCTAATCTTCCTGTTGAAAGGAAGCCGCTTGTTTGCGTACTTGGAGTGCCGAATCACATGACATATGCTGACTTCTGTAGATTCTGTGGTTCCTTCATTCAGCATATATTGGAGATGCGGATTGTCAGGACCGATGGAATGGAGGATCGTTATAGCATTTTGATTAGGTTTGATAGTCAGGACTCCACAGATCATTTTTACAAGCACTTCAATCGGACCCGGTTCTCATCACTAGAGGTGGAGGTCTGTCGCATACTTTTCACGGTAGATGTTCAATACACTGGTTCAATAGAACATGCACAGGCATCCCTCTGTAGCTCAACTGAGCAGCCATCATGTCCAGTCTGTCTTGAGAGACTAGACCAAGATATGAGTGGAATTCTGACAACTATCTGCAATCATTCTTTCCATTGCTCTTGCATTTCAAAATGGACCGATTCTTCTTGTCCTGTATGTCGATATTGTCAGCAGCAGCCTGAAAAGTCCAACTGTTTCATTTGTCAAACTTCTGAGAATCTCTGGATGTGTATTATCTGTGGCTTTGTTGGTTGTGGGAGGTATAAAGAAGGGCATGCCATAAGACATTGGAAAGAAACACAACATTGCTATTCCCTTGAAGTGGAAACGCAGCGTGTGTGGGATTATGCAGGAGACAATTATGTTCACCGCCTAATTCAATCTAAAACTGATGGAAAATTGGTTGAATTGAATTCACACTGTCGGAATGCGAATGATTTCAAAGGAAGTTGCGAGTGTGTAGATTCTGGATTCAGTGAGGCTCTTTTGAACAGTAAAGTAGAAGCTGTAATGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
BRAP2 zf-RING_2 zf-UBP

Protein Analysis

348

Amino Acids

39.16

Weight (kDa)

5.64

Isoelectric Point (pI)

53.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BRAP2 PF07576 80 - 177 1.7e-38 BRCA1-associated protein 2
zf-rbx1 PF12678 180 - 230 2.6e-07 RING-H2 zinc finger domain
zf-RING_2 PF13639 189 - 230 1.1e-08 Ring finger domain
zf-UBP PF02148 241 - 300 5.5e-23 Zn-finger in ubiquitin-hydrolases and other protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013575)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26000 AT2G26000 AT2G26000
fragaria_vesca FvH4_6g29040
malus_domestica MD17G1218900.v1.1
prunus_persica Prupe.3G094400_v2.0.a1 Prupe.3G094400_v2.0.a1
pyrus_communis pycom17g22370
rosa_chinensis RchiOBHm_Chr2g0133711
rosa_laevigata RLG00000019358
rosa_multiflora Rmu_sc0013154.1_g000008
rosa_roxburghii Rroxscaffold_2G00110880
rosa_rugosa Rorug02G0313200
rosa_samantha Rh2AG366600 Rh2BG372300 Rh2CG349900 Rh2DG389200
rosa_wichuraiana Rw2G029830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 212
AatII GACGTC 1 cut(s) 216
AccII CGCG 1 cut(s) 204
AciI CCGC 5 cut(s) 62, 202, 257, 349, 898
AclWI GGATC 1 cut(s) 384
AcsI RAATTY 2 cut(s) 609, 940
AcuI CTGAAG 1 cut(s) 37
AcvI CACGTG 1 cut(s) 26
AcyI GRCGYC 1 cut(s) 213
AfaI GTAC 1 cut(s) 270
AfiI CCNNNNNNNGG 1 cut(s) 850
AluBI AGCT 3 cut(s) 12, 549, 1034
AluI AGCT 3 cut(s) 12, 549, 1034
Alw21I GWGCWC 2 cut(s) 14, 20
Alw26I GTCTC 4 cut(s) 170, 223, 580, 876
AlwI GGATC 1 cut(s) 384
Ama87I CYCGRG 1 cut(s) 13
AoxI GGCC 2 cut(s) 48, 138
ApeKI GCWGC 4 cut(s) 559, 700, 703, 859
ApoI RAATTY 2 cut(s) 609, 940
Asp700I GAANNNNTTC 1 cut(s) 965
AspS9I GGNCC 3 cut(s) 360, 447, 663
AsuC2I CCSGG 2 cut(s) 78, 451
AsuHPI GGTGA 1 cut(s) 887
AvaI CYCGRG 1 cut(s) 13
AvaII GGWCC 3 cut(s) 360, 447, 663
BanII GRGCYC 1 cut(s) 14
BbrPI CACGTG 1 cut(s) 26
Bbv12I GWGCWC 2 cut(s) 14, 20
BbvI GCAGC 4 cut(s) 571, 712, 715, 871
BccI CCATC 4 cut(s) 128, 359, 571, 914
BcgI CGANNNNNNTGC 2 cut(s) 425, 459
BcnI CCSGG 2 cut(s) 78, 451
BcoDI GTCTC 4 cut(s) 170, 223, 580, 876
BfaI CTAG 4 cut(s) 87, 165, 464, 590
BfmI CTRYAG 2 cut(s) 307, 544
BisI GCNGC 6 cut(s) 62, 257, 560, 701, 704, 860
BlsI GCNGC 6 cut(s) 63, 258, 561, 702, 705, 861
Bme1390I CCNGG 2 cut(s) 78, 451
Bme18I GGWCC 3 cut(s) 360, 447, 663
BmeT110I CYCGRG 1 cut(s) 13
BmgT120I GGNCC 3 cut(s) 360, 447, 663
BmiI GGNNCC 3 cut(s) 34, 322, 449
BmrFI CCNGG 2 cut(s) 78, 451
BmsI GCATC 3 cut(s) 195, 336, 545
BpuEI CTTGAG 1 cut(s) 602
BpuMI CCSGG 2 cut(s) 78, 451
BsaAI YACGTR 1 cut(s) 26
BsaHI GRCGYC 1 cut(s) 213
BsaI GGTCTC 1 cut(s) 170
Bsc4I CCNNNNNNNGG 1 cut(s) 850
Bse118I RCCGGY 1 cut(s) 40
Bse1I ACTGG 2 cut(s) 517, 572
Bse3DI GCAATG 2 cut(s) 640, 832
BseGI GGATG 3 cut(s) 113, 536, 759
BseLI CCNNNNNNNGG 1 cut(s) 850
BseMI GCAATG 2 cut(s) 640, 832
BseMII CTCAG 2 cut(s) 546, 732
BseNI ACTGG 2 cut(s) 517, 572
BseRI GAGGAG 2 cut(s) 97, 185
BseXI GCAGC 4 cut(s) 571, 712, 715, 871
Bsh1236I CGCG 1 cut(s) 204
Bsh1285I CGRYCG 1 cut(s) 45
BshFI GGCC 2 cut(s) 50, 140
BsiEI CGRYCG 1 cut(s) 45
BsiHKAI GWGCWC 2 cut(s) 14, 20
BsiHKCI CYCGRG 1 cut(s) 13
BsiSI CCGG 4 cut(s) 30, 41, 78, 451
BslI CCNNNNNNNGG 1 cut(s) 850
BsmAI GTCTC 4 cut(s) 170, 223, 580, 876
BsmBI CGTCTC 1 cut(s) 223
BsmI GAATGC 1 cut(s) 961
BsnI GGCC 2 cut(s) 50, 140
Bso31I GGTCTC 1 cut(s) 170
BsoBI CYCGRG 1 cut(s) 13
Bsp1286I GDGCHC 2 cut(s) 14, 20
Bsp143I GATC 2 cut(s) 376, 421
BspACI CCGC 5 cut(s) 62, 202, 257, 349, 898
BspANI GGCC 2 cut(s) 50, 140
BspCNI CTCAG 2 cut(s) 547, 733
BspFNI CGCG 1 cut(s) 204
BspLI GGNNCC 3 cut(s) 34, 322, 449
BspPI GGATC 1 cut(s) 384
BspTNI GGTCTC 1 cut(s) 170
BsrDI GCAATG 2 cut(s) 640, 832
BsrFI RCCGGY 1 cut(s) 40
BsrI ACTGG 2 cut(s) 517, 572
BssAI RCCGGY 1 cut(s) 40
BssMI GATC 2 cut(s) 376, 421
BssNI GRCGYC 1 cut(s) 213
Bst4CI ACNGT 4 cut(s) 496, 722, 950, 1022
Bst6I CTCTTC 3 cut(s) 115, 157, 168
BstACI GRCGYC 1 cut(s) 213
BstBAI YACGTR 1 cut(s) 26
BstC8I GCNNGC 3 cut(s) 83, 138, 804
BstDEI CTNAG 2 cut(s) 555, 741
BstF5I GGATG 3 cut(s) 113, 536, 759
BstFNI CGCG 1 cut(s) 204
BstKTI GATC 2 cut(s) 379, 424
BstMAI GTCTC 4 cut(s) 170, 223, 580, 876
BstMBI GATC 2 cut(s) 376, 421
BstMCI CGRYCG 1 cut(s) 45
BstMWI GCNNNNNNNGC 1 cut(s) 87
BstNSI RCATGY 2 cut(s) 530, 806
BstSCI CCNGG 2 cut(s) 76, 449
BstSFI CTRYAG 2 cut(s) 307, 544
BstUI CGCG 1 cut(s) 204
BstV1I GCAGC 4 cut(s) 571, 712, 715, 871
BsuRI GGCC 2 cut(s) 50, 140
BtsCI GGATG 3 cut(s) 113, 536, 759
BtsIMutI CAGTG 3 cut(s) 510, 946, 1009
Cac8I GCNNGC 3 cut(s) 83, 138, 804
Cfr10I RCCGGY 1 cut(s) 40
Cfr13I GGNCC 3 cut(s) 360, 447, 663
Csp6I GTAC 1 cut(s) 269
CviAII CATG 5 cut(s) 190, 289, 527, 567, 803
CviQI GTAC 1 cut(s) 269
DdeI CTNAG 2 cut(s) 555, 741
DpnI GATC 2 cut(s) 378, 423
DpnII GATC 2 cut(s) 376, 421
DrdI GACNNNNNNGTC 1 cut(s) 212
DseDI GACNNNNNNGTC 1 cut(s) 212
Eam1104I CTCTTC 3 cut(s) 115, 157, 168
EarI CTCTTC 3 cut(s) 115, 157, 168
Ecl136II GAGCTC 1 cut(s) 12
Eco147I AGGCCT 2 cut(s) 50, 140
Eco24I GRGCYC 1 cut(s) 14
Eco31I GGTCTC 1 cut(s) 170
Eco47I GGWCC 3 cut(s) 360, 447, 663
Eco53kI GAGCTC 1 cut(s) 12
Eco57I CTGAAG 1 cut(s) 37
Eco72I CACGTG 1 cut(s) 26
Eco88I CYCGRG 1 cut(s) 13
EcoICRI GAGCTC 1 cut(s) 12
EcoRI GAATTC 2 cut(s) 609, 940
EcoT38I GRGCYC 1 cut(s) 14
Esp3I CGTCTC 1 cut(s) 223
FaeI CATG 5 cut(s) 193, 292, 530, 570, 806
FatI CATG 5 cut(s) 189, 288, 526, 566, 802
FauNDI CATATG 1 cut(s) 295
Fnu4HI GCNGC 6 cut(s) 62, 257, 560, 701, 704, 860
FokI GGATG 3 cut(s) 100, 523, 766
FriOI GRGCYC 1 cut(s) 14
Fsp4HI GCNGC 6 cut(s) 62, 257, 560, 701, 704, 860
FspBI CTAG 4 cut(s) 87, 165, 464, 590
GluI GCNGC 6 cut(s) 62, 257, 560, 701, 704, 860
HaeIII GGCC 2 cut(s) 50, 140
HapII CCGG 4 cut(s) 30, 41, 78, 451
Hin1I GRCGYC 1 cut(s) 213
Hin1II CATG 5 cut(s) 193, 292, 530, 570, 806
HinfI GANTC 7 cut(s) 283, 312, 413, 668, 745, 992, 999
HpaII CCGG 4 cut(s) 30, 41, 78, 451
HphI GGTGA 1 cut(s) 887
Hpy166II GTNNAC 1 cut(s) 895
Hpy188I TCNGA 4 cut(s) 447, 615, 742, 954
Hpy188III TCNNGA 6 cut(s) 156, 358, 410, 581, 751, 996
Hpy8I GTNNAC 1 cut(s) 895
Hpy99I CGWCG 2 cut(s) 209, 218
HpyAV CCTTC 4 cut(s) 61, 75, 334, 791
HpyCH4III ACNGT 4 cut(s) 496, 722, 950, 1022
HpyCH4IV ACGT 2 cut(s) 25, 213
HpyCH4V TGCA 4 cut(s) 530, 627, 651, 878
HpyF10VI GCNNNNNNNGC 1 cut(s) 87
HpyF3I CTNAG 2 cut(s) 555, 741
HpySE526I ACGT 2 cut(s) 25, 213
Hsp92I GRCGYC 1 cut(s) 213
Hsp92II CATG 5 cut(s) 193, 292, 530, 570, 806
Kzo9I GATC 2 cut(s) 376, 421
LmnI GCTCC 2 cut(s) 32, 198
Lsp1109I GCAGC 4 cut(s) 571, 712, 715, 871
LweI GCATC 3 cut(s) 195, 336, 545
MaeI CTAG 4 cut(s) 87, 165, 464, 590
MaeII ACGT 2 cut(s) 25, 213
MalI GATC 2 cut(s) 378, 423
MboI GATC 2 cut(s) 376, 421
MboII GAAGA 5 cut(s) 102, 144, 185, 230, 663
MhlI GDGCHC 2 cut(s) 14, 20
MluCI AATT 7 cut(s) 168, 609, 886, 903, 926, 935, 940
MlyI GAGTC 1 cut(s) 407
MmeI TCCRAC 2 cut(s) 741, 932
MroXI GAANNNNTTC 1 cut(s) 965
MseI TTAA 1 cut(s) 1045
MspI CCGG 4 cut(s) 30, 41, 78, 451
MspR9I CCNGG 2 cut(s) 78, 451
Mva1269I GAATGC 1 cut(s) 961
MvnI CGCG 1 cut(s) 204
MwoI GCNNNNNNNGC 1 cut(s) 87
NciI CCSGG 2 cut(s) 78, 451
NdeI CATATG 1 cut(s) 295
NdeII GATC 2 cut(s) 376, 421
NlaIII CATG 5 cut(s) 193, 292, 530, 570, 806
NlaIV GGNNCC 3 cut(s) 34, 322, 449
NmeAIII GCCGAG 1 cut(s) 33
NspI RCATGY 2 cut(s) 530, 806
PaeI GCATGC 1 cut(s) 806
PaeR7I CTCGAG 1 cut(s) 13
PceI AGGCCT 2 cut(s) 50, 140
PcsI WCGNNNNNNNCGW 4 cut(s) 11, 65, 213, 227
PctI GAATGC 1 cut(s) 961
PdmI GAANNNNTTC 1 cut(s) 965
PfeI GAWTC 6 cut(s) 283, 312, 668, 745, 992, 999
PflFI GACNNNGTC 1 cut(s) 215
PkrI GCNGC 6 cut(s) 63, 258, 561, 702, 705, 861
PleI GAGTC 1 cut(s) 407
PmaCI CACGTG 1 cut(s) 26
PmlI CACGTG 1 cut(s) 26
PpsI GAGTC 1 cut(s) 407
Ppu21I YACGTR 1 cut(s) 26
Psp124BI GAGCTC 1 cut(s) 14
PspCI CACGTG 1 cut(s) 26
PspN4I GGNNCC 3 cut(s) 34, 322, 449
PspPI GGNCC 3 cut(s) 360, 447, 663
PspXI VCTCGAGB 1 cut(s) 13
PsyI GACNNNGTC 1 cut(s) 215
RsaI GTAC 1 cut(s) 270
RsaNI GTAC 1 cut(s) 269
SacI GAGCTC 1 cut(s) 14
SaqAI TTAA 1 cut(s) 1045
SatI GCNGC 6 cut(s) 62, 257, 560, 701, 704, 860
Sau3AI GATC 2 cut(s) 376, 421
Sau96I GGNCC 3 cut(s) 360, 447, 663
SchI GAGTC 1 cut(s) 407
ScrFI CCNGG 2 cut(s) 78, 451
SduI GDGCHC 2 cut(s) 14, 20
SfaNI GCATC 3 cut(s) 195, 336, 545
SfcI CTRYAG 2 cut(s) 307, 544
Sfr274I CTCGAG 1 cut(s) 13
SinI GGWCC 3 cut(s) 360, 447, 663
SlaI CTCGAG 1 cut(s) 13
SmlI CTYRAG 2 cut(s) 13, 581
SmoI CTYRAG 2 cut(s) 13, 581
SphI GCATGC 1 cut(s) 806
Sse9I AATT 7 cut(s) 168, 609, 886, 903, 926, 935, 940
SseBI AGGCCT 2 cut(s) 50, 140
SsiI CCGC 5 cut(s) 62, 202, 257, 349, 898
SspMI CTAG 4 cut(s) 87, 165, 464, 590
SstI GAGCTC 1 cut(s) 14
StuI AGGCCT 2 cut(s) 50, 140
StyD4I CCNGG 2 cut(s) 76, 449
TaaI ACNGT 4 cut(s) 496, 722, 950, 1022
TaiI ACGT 2 cut(s) 28, 216
TaqI TCGA 4 cut(s) 14, 45, 68, 688
TaqII GACCGA 2 cut(s) 377, 680
TasI AATT 7 cut(s) 168, 609, 886, 903, 926, 935, 940
TauI GCSGC 2 cut(s) 64, 259
TfiI GAWTC 6 cut(s) 283, 312, 668, 745, 992, 999
Tru1I TTAA 1 cut(s) 1045
Tru9I TTAA 1 cut(s) 1045
TscAI CASTG 3 cut(s) 517, 953, 1009
TseI GCWGC 4 cut(s) 559, 700, 703, 859
TspDTI ATGAA 3 cut(s) 102, 316, 715
TspGWI ACGGA 1 cut(s) 219
TspRI CASTG 3 cut(s) 517, 953, 1009
Tth111I GACNNNGTC 1 cut(s) 215
VpaK11BI GGWCC 3 cut(s) 360, 447, 663
XapI RAATTY 2 cut(s) 609, 940
XceI RCATGY 2 cut(s) 530, 806
XcmI CCANNNNNNNNNTGG 1 cut(s) 602
XhoI CTCGAG 1 cut(s) 13
XmnI GAANNNNTTC 1 cut(s) 965
XspI CTAG 4 cut(s) 87, 165, 464, 590
ZraI GACGTC 1 cut(s) 214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.