Rorug02G0313200

Brca1-associated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0013575
Physical Location & Seq
Reverse (-)
37924747 .. 37926162
1416 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0313200.1

Sequence Viewer

Length: 1416 bp
ATGGCCACAGCAAAAAGGAGTGGTGAATCAAACATTGAAACTCGGATGGAAACAAGTCGAGATTGTGACCTTGACAAGGGCAAGGAACGTGATTTTTTTTTGGAGAGTTGTCAGTGGAAGCCGGTTTTTGACGAAGCTTCAATGTCACATAGACCTCTTAAGAAGATCAAGAGCCCTGAACGTAGTCATCAGCACCAATCCTCTGCTTTTCCCCCTGAATTTCATATCCCATCTTCTCCTCCTCGTAGTTCATCAGATACATCATCAATCACTTCTTGTTGTCCTTCTTCTTCCAGAATAGTCTTTCCTTTTGCTTTTGATGGGTCCCTGCCTCAGCAGCAGCAGCAACCTATACAATTTCCCCACCAATTCCAGACCACAGGTCTGCAGCCTATACAATTTCCGCCAAGTACACATGTTGTAGAGCATCAGCATCAGCAGCAGCAACAACAAAATCAGCAGCAGATGATATCTTTCGCATCGCAGCAGCAACAAATGATATCTTGGCGGCAGCAGCAGCAGCAGCAGCAACAGCAAATGCTTCAGTATTGGAGTGATGCTTTGAATTTGAGTCCAAGAGGAAGGATGATGATGATGAACAGGTTTGGAGGAGCTAATAGTAGTGGTGGTATTAGGCCAGCGGCGATGCCTGTAAGTACTAGTAAGCTTTACAGGGGAGTAAGGCAAAGGCATTGGGGCAAATGGGTAGCTGAAATTCGTCTTCCTCGAAACAGGACTCGCCTCTGGCTGGGCACCTTCGACACCGCTGAAGATGCTGCCATGGCTTATGACCGTGAGGCTTTCAAGTTGAGAGGAGAGAATGCTAGGCTTAATTTCCCTGAGCTTTTCCTCAACAAAGACAAAGATAATAATAATGCTGCAGCTGTTTCCACAGCTCCAAGTTCAACCGAAACTGCTTCTGTGCCGACTCAACAAGTTTCAAAGCCATCCAGGCACCAGGGTAAGCCGCAACTGAAAAACCTTGATTTACAATTACAGGTCCCAATTTCAGACAGGGAAACAATTGCACCTCCACCAGCGCCATATTCACATCCCCAGGAAGACGATGACACTGGTGAAAGTGATTTGGCCAATGCCAGCCAGCAATCTGAGGTTGAAAATCATGGCGAAGGAGGAGGGATCATTTCAGATGCCCCAACACAGGAAATGGCTTGGGGAGAAATGGCAGAGGCTTGGATGAATGCAATTCCACAAGGTTGGGGTCCTGGTAGTCCTGTCTGGGATGATTTAGAGTTGGACATGACTTCCAACAATCTTGTGCTGCAATCTCATGATTATCACCATTTTACGGCCAATAATCCGAGCCGGCATGAACTTCCTCAGCAGGAAGACAACTCTTGTTCTGCTTCATCTTCATATCCCATGCAGCCCTTCTTTTGGAAAGATCAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
BRAP2 zf-RING_2 zf-UBP

Protein Analysis

471

Amino Acids

53.27

Weight (kDa)

5.74

Isoelectric Point (pI)

68.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 223 - 272 1.9e-13 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013575)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26000 AT2G26000 AT2G26000
fragaria_vesca FvH4_6g29040
malus_domestica MD17G1218900.v1.1
prunus_persica Prupe.3G094400_v2.0.a1 Prupe.3G094400_v2.0.a1
pyrus_communis pycom17g22370
rosa_chinensis RchiOBHm_Chr2g0133711
rosa_laevigata RLG00000019358
rosa_multiflora Rmu_sc0013154.1_g000008
rosa_roxburghii Rroxscaffold_2G00110880
rosa_rugosa Rorug02G0313200
rosa_samantha Rh2AG366600 Rh2BG372300 Rh2CG349900 Rh2DG389200
rosa_wichuraiana Rw2G029830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 752, 954
AciI CCGC 5 cut(s) 404, 508, 641, 765, 968
AclWI GGATC 1 cut(s) 1148
AcoI YGGCCR 3 cut(s) 3, 1089, 1311
AcsI RAATTY 3 cut(s) 218, 565, 714
AcuI CTGAAG 2 cut(s) 527, 789
AfaI GTAC 2 cut(s) 412, 658
AfiI CCNNNNNNNGG 5 cut(s) 76, 748, 1162, 1309, 1398
AflII CTTAAG 1 cut(s) 158
AflIII ACRYGT 1 cut(s) 415
AgsI TTSAA 7 cut(s) 38, 141, 565, 805, 906, 942, 1118
AhdI GACNNNNNGTC 1 cut(s) 381
AhlI ACTAGT 1 cut(s) 659
AjnI CCWGG 4 cut(s) 950, 957, 1056, 1225
AluBI AGCT 7 cut(s) 137, 614, 667, 710, 844, 884, 896
AluI AGCT 7 cut(s) 137, 614, 667, 710, 844, 884, 896
AlwI GGATC 1 cut(s) 1148
AoxI GGCC 4 cut(s) 3, 635, 1089, 1311
ApoI RAATTY 3 cut(s) 218, 565, 714
AspLEI GCGC 1 cut(s) 1042
AspS9I GGNCC 3 cut(s) 324, 1000, 1223
AsuHPI GGTGA 3 cut(s) 35, 1088, 1292
AvaII GGWCC 3 cut(s) 324, 1000, 1223
BaeGI GKGCMC 1 cut(s) 755
BalI TGGCCA 2 cut(s) 5, 1091
BanI GGYRCC 2 cut(s) 752, 954
BanII GRGCYC 1 cut(s) 176
BbsI GAAGAC 3 cut(s) 713, 1068, 1356
BbvCI CCTCAGC 2 cut(s) 333, 1341
BccI CCATC 4 cut(s) 40, 238, 314, 955
BceAI ACGGC 1 cut(s) 1326
BciT130I CCWGG 4 cut(s) 952, 959, 1058, 1227
BcuI ACTAGT 1 cut(s) 659
BfaI CTAG 2 cut(s) 660, 825
BfmI CTRYAG 2 cut(s) 386, 879
BfoI RGCGCY 1 cut(s) 1043
BfrI CTTAAG 1 cut(s) 158
BglI GCCNNNNNGGC 1 cut(s) 952
BmcAI AGTACT 1 cut(s) 658
Bme1390I CCNGG 4 cut(s) 952, 959, 1058, 1227
Bme18I GGWCC 3 cut(s) 324, 1000, 1223
BmeRI GACNNNNNGTC 1 cut(s) 381
BmgT120I GGNCC 3 cut(s) 324, 1000, 1223
BmiI GGNNCC 6 cut(s) 325, 326, 754, 956, 1002, 1224
BmrFI CCNGG 4 cut(s) 952, 959, 1058, 1227
BmsI GCATC 7 cut(s) 436, 442, 488, 547, 636, 763, 1141
BpiI GAAGAC 3 cut(s) 713, 1068, 1356
Bpu10I CCTNAGC 3 cut(s) 333, 840, 1341
BsaJI CCNNGG 3 cut(s) 780, 958, 1056
Bsc4I CCNNNNNNNGG 5 cut(s) 76, 748, 1162, 1309, 1398
Bse118I RCCGGY 2 cut(s) 121, 1326
Bse1I ACTGG 1 cut(s) 1078
BseBI CCWGG 4 cut(s) 952, 959, 1058, 1227
BseDI CCNNGG 3 cut(s) 780, 958, 1056
BseGI GGATG 6 cut(s) 51, 591, 947, 1051, 1203, 1249
BseLI CCNNNNNNNGG 5 cut(s) 76, 748, 1162, 1309, 1398
BseMII CTCAG 4 cut(s) 347, 831, 1101, 1355
BseNI ACTGG 1 cut(s) 1078
BseRI GAGGAG 5 cut(s) 228, 231, 624, 828, 1149
BseSI GKGCMC 1 cut(s) 755
BseYI CCCAGC 1 cut(s) 748
BshFI GGCC 4 cut(s) 5, 637, 1091, 1313
BshNI GGYRCC 2 cut(s) 752, 954
BsiSI CCGG 2 cut(s) 122, 1327
BslFI GGGAC 2 cut(s) 310, 986
BslI CCNNNNNNNGG 5 cut(s) 76, 748, 1162, 1309, 1398
BsmFI GGGAC 2 cut(s) 310, 986
BsmI GAATGC 2 cut(s) 826, 1207
BsnI GGCC 4 cut(s) 5, 637, 1091, 1313
Bsp1286I GDGCHC 2 cut(s) 176, 755
Bsp143I GATC 3 cut(s) 165, 1140, 1405
Bsp19I CCATGG 1 cut(s) 780
BspACI CCGC 5 cut(s) 404, 508, 641, 765, 968
BspANI GGCC 4 cut(s) 5, 637, 1091, 1313
BspCNI CTCAG 4 cut(s) 346, 832, 1102, 1354
BspHI TCATGA 1 cut(s) 1291
BspLI GGNNCC 6 cut(s) 325, 326, 754, 956, 1002, 1224
BspMAI CTGCAG 2 cut(s) 390, 883
BspPI GGATC 1 cut(s) 1148
BspT107I GGYRCC 2 cut(s) 752, 954
BspTI CTTAAG 1 cut(s) 158
BsrFI RCCGGY 2 cut(s) 121, 1326
BsrI ACTGG 1 cut(s) 1078
BssAI RCCGGY 2 cut(s) 121, 1326
BssECI CCNNGG 3 cut(s) 780, 958, 1056
BssMI GATC 3 cut(s) 165, 1140, 1405
BssT1I CCWWGG 1 cut(s) 780
Bst2UI CCWGG 4 cut(s) 952, 959, 1058, 1227
Bst4CI ACNGT 1 cut(s) 794
BstAFI CTTAAG 1 cut(s) 158
BstC8I GCNNGC 4 cut(s) 639, 1099, 1103, 1328
BstDEI CTNAG 4 cut(s) 333, 840, 1110, 1341
BstDSI CCRYGG 1 cut(s) 780
BstENI CCTNNNNNAGG 1 cut(s) 74
BstF5I GGATG 6 cut(s) 51, 591, 947, 1051, 1203, 1249
BstH2I RGCGCY 1 cut(s) 1043
BstHHI GCGC 1 cut(s) 1042
BstKTI GATC 3 cut(s) 168, 1143, 1408
BstMBI GATC 3 cut(s) 165, 1140, 1405
BstNI CCWGG 4 cut(s) 952, 959, 1058, 1227
BstNSI RCATGY 1 cut(s) 419
BstSCI CCNGG 4 cut(s) 950, 957, 1056, 1225
BstSFI CTRYAG 2 cut(s) 386, 879
BstSLI GKGCMC 1 cut(s) 755
BstV2I GAAGAC 3 cut(s) 713, 1068, 1356
BstXI CCANNNNNNTGG 1 cut(s) 1218
BsuRI GGCC 4 cut(s) 5, 637, 1091, 1313
BtgI CCRYGG 1 cut(s) 780
BtgZI GCGATG 2 cut(s) 465, 659
BtsCI GGATG 6 cut(s) 51, 591, 947, 1051, 1203, 1249
BtsIMutI CAGTG 2 cut(s) 119, 1071
Cac8I GCNNGC 4 cut(s) 639, 1099, 1103, 1328
CciI TCATGA 1 cut(s) 1291
CfoI GCGC 1 cut(s) 1042
Cfr10I RCCGGY 2 cut(s) 121, 1326
Cfr13I GGNCC 3 cut(s) 324, 1000, 1223
Csp6I GTAC 2 cut(s) 411, 657
CviAII CATG 7 cut(s) 416, 781, 1124, 1261, 1292, 1331, 1384
CviQI GTAC 2 cut(s) 411, 657
DdeI CTNAG 4 cut(s) 333, 840, 1110, 1341
DpnI GATC 3 cut(s) 167, 1142, 1407
DpnII GATC 3 cut(s) 165, 1140, 1405
DriI GACNNNNNGTC 1 cut(s) 381
EaeI YGGCCR 3 cut(s) 3, 1089, 1311
Eam1105I GACNNNNNGTC 1 cut(s) 381
EciI GGCGGA 1 cut(s) 393
Eco130I CCWWGG 1 cut(s) 780
Eco24I GRGCYC 1 cut(s) 176
Eco32I GATATC 2 cut(s) 471, 501
Eco47I GGWCC 3 cut(s) 324, 1000, 1223
Eco57I CTGAAG 2 cut(s) 527, 789
EcoNI CCTNNNNNAGG 1 cut(s) 74
EcoO109I RGGNCCY 3 cut(s) 324, 1000, 1223
EcoRII CCWGG 4 cut(s) 950, 957, 1056, 1225
EcoRV GATATC 2 cut(s) 471, 501
EcoT14I CCWWGG 1 cut(s) 780
EcoT38I GRGCYC 1 cut(s) 176
ErhI CCWWGG 1 cut(s) 780
FaeI CATG 7 cut(s) 419, 784, 1127, 1264, 1295, 1334, 1387
FaqI GGGAC 2 cut(s) 310, 986
FatI CATG 7 cut(s) 415, 780, 1123, 1260, 1291, 1330, 1383
FokI GGATG 6 cut(s) 58, 598, 934, 1038, 1210, 1256
FriOI GRGCYC 1 cut(s) 176
FspBI CTAG 2 cut(s) 660, 825
GlaI GCGC 1 cut(s) 1041
GsaI CCCAGC 1 cut(s) 752
HaeII RGCGCY 1 cut(s) 1043
HaeIII GGCC 4 cut(s) 5, 637, 1091, 1313
HapII CCGG 2 cut(s) 122, 1327
HhaI GCGC 1 cut(s) 1042
Hin1II CATG 7 cut(s) 419, 784, 1127, 1264, 1295, 1334, 1387
Hin6I GCGC 1 cut(s) 1040
HinP1I GCGC 1 cut(s) 1040
HindIII AAGCTT 2 cut(s) 135, 665
HinfI GANTC 4 cut(s) 26, 571, 736, 928
HpaII CCGG 2 cut(s) 122, 1327
HphI GGTGA 3 cut(s) 35, 1088, 1292
Hpy166II GTNNAC 1 cut(s) 413
Hpy188I TCNGA 6 cut(s) 45, 256, 1012, 1111, 1150, 1323
Hpy188III TCNNGA 6 cut(s) 59, 169, 294, 373, 1292, 1409
Hpy8I GTNNAC 1 cut(s) 413
HpyAV CCTTC 5 cut(s) 294, 576, 766, 1124, 1402
HpyCH4III ACNGT 1 cut(s) 794
HpyCH4IV ACGT 2 cut(s) 88, 181
HpyCH4V TGCA 6 cut(s) 388, 881, 1028, 1205, 1285, 1387
HpyF3I CTNAG 4 cut(s) 333, 840, 1110, 1341
HpySE526I ACGT 2 cut(s) 88, 181
Hsp92II CATG 7 cut(s) 419, 784, 1127, 1264, 1295, 1334, 1387
HspAI GCGC 1 cut(s) 1040
KflI GGGWCCC 1 cut(s) 324
KroI GCCGGC 1 cut(s) 1326
KroNI GCCGGC 1 cut(s) 1328
Kzo9I GATC 3 cut(s) 165, 1140, 1405
LmnI GCTCC 2 cut(s) 611, 901
LweI GCATC 7 cut(s) 436, 442, 488, 547, 636, 763, 1141
MaeI CTAG 2 cut(s) 660, 825
MaeII ACGT 2 cut(s) 88, 181
MaeIII GTNAC 2 cut(s) 65, 144
MalI GATC 3 cut(s) 167, 1142, 1407
MboI GATC 3 cut(s) 165, 1140, 1405
MboII GAAGA 9 cut(s) 175, 225, 279, 282, 713, 782, 1073, 1361, 1365
MfeI CAATTG 1 cut(s) 1023
MhlI GDGCHC 2 cut(s) 176, 755
MlsI TGGCCA 2 cut(s) 5, 1091
MluNI TGGCCA 2 cut(s) 5, 1091
MlyI GAGTC 3 cut(s) 580, 730, 922
MmeI TCCRAC 2 cut(s) 1236, 1293
Mox20I TGGCCA 2 cut(s) 5, 1091
MroNI GCCGGC 1 cut(s) 1326
MscI TGGCCA 2 cut(s) 5, 1091
MseI TTAA 2 cut(s) 159, 831
Msp20I TGGCCA 2 cut(s) 5, 1091
MspA1I CMGCKG 3 cut(s) 641, 767, 884
MspCI CTTAAG 1 cut(s) 158
MspI CCGG 2 cut(s) 122, 1327
MspR9I CCNGG 4 cut(s) 952, 959, 1058, 1227
MunI CAATTG 1 cut(s) 1023
Mva1269I GAATGC 2 cut(s) 826, 1207
MvaI CCWGG 4 cut(s) 952, 959, 1058, 1227
NaeI GCCGGC 1 cut(s) 1328
NcoI CCATGG 1 cut(s) 780
NdeII GATC 3 cut(s) 165, 1140, 1405
NgoMIV GCCGGC 1 cut(s) 1326
NlaIII CATG 7 cut(s) 419, 784, 1127, 1264, 1295, 1334, 1387
NlaIV GGNNCC 6 cut(s) 325, 326, 754, 956, 1002, 1224
NmuCI GTSAC 2 cut(s) 65, 144
NspI RCATGY 1 cut(s) 419
PagI TCATGA 1 cut(s) 1291
PciI ACATGT 1 cut(s) 415
PcsI WCGNNNNNNNCGW 1 cut(s) 724
PctI GAATGC 2 cut(s) 826, 1207
PdiI GCCGGC 1 cut(s) 1328
PfeI GAWTC 1 cut(s) 26
PleI GAGTC 3 cut(s) 579, 730, 922
PpsI GAGTC 3 cut(s) 579, 730, 922
PpuMI RGGWCCY 3 cut(s) 324, 1000, 1223
PscI ACATGT 1 cut(s) 415
Psp5II RGGWCCY 3 cut(s) 324, 1000, 1223
Psp6I CCWGG 4 cut(s) 950, 957, 1056, 1225
PspFI CCCAGC 1 cut(s) 748
PspGI CCWGG 4 cut(s) 950, 957, 1056, 1225
PspN4I GGNNCC 6 cut(s) 325, 326, 754, 956, 1002, 1224
PspPI GGNCC 3 cut(s) 324, 1000, 1223
PspPPI RGGWCCY 3 cut(s) 324, 1000, 1223
PstI CTGCAG 2 cut(s) 390, 883
PvuII CAGCTG 1 cut(s) 884
RsaI GTAC 2 cut(s) 412, 658
RsaNI GTAC 2 cut(s) 411, 657
SaqAI TTAA 2 cut(s) 159, 831
Sau3AI GATC 3 cut(s) 165, 1140, 1405
Sau96I GGNCC 3 cut(s) 324, 1000, 1223
ScaI AGTACT 1 cut(s) 658
SchI GAGTC 3 cut(s) 580, 730, 922
ScrFI CCNGG 4 cut(s) 952, 959, 1058, 1227
SduI GDGCHC 2 cut(s) 176, 755
SfaNI GCATC 7 cut(s) 436, 442, 488, 547, 636, 763, 1141
SfcI CTRYAG 2 cut(s) 386, 879
SinI GGWCC 3 cut(s) 324, 1000, 1223
SmlI CTYRAG 1 cut(s) 158
SmoI CTYRAG 1 cut(s) 158
SpeI ACTAGT 1 cut(s) 659
SsiI CCGC 5 cut(s) 404, 508, 641, 765, 968
SspMI CTAG 2 cut(s) 660, 825
StyD4I CCNGG 4 cut(s) 950, 957, 1056, 1225
StyI CCWWGG 1 cut(s) 780
TaaI ACNGT 1 cut(s) 794
TaiI ACGT 2 cut(s) 91, 184
TaqI TCGA 3 cut(s) 58, 727, 759
TatI WGTACW 2 cut(s) 410, 656
TauI GCSGC 3 cut(s) 511, 644, 970
TfiI GAWTC 1 cut(s) 26
Tru1I TTAA 2 cut(s) 159, 831
Tru9I TTAA 2 cut(s) 159, 831
TscAI CASTG 2 cut(s) 119, 1078
TseFI GTSAC 2 cut(s) 65, 144
Tsp45I GTSAC 2 cut(s) 65, 144
TspDTI ATGAA 7 cut(s) 212, 240, 611, 1214, 1347, 1359, 1365
TspRI CASTG 2 cut(s) 119, 1078
Vha464I CTTAAG 1 cut(s) 158
VpaK11BI GGWCC 3 cut(s) 324, 1000, 1223
XagI CCTNNNNNAGG 1 cut(s) 74
XapI RAATTY 3 cut(s) 218, 565, 714
XceI RCATGY 1 cut(s) 419
XspI CTAG 2 cut(s) 660, 825
ZrmI AGTACT 1 cut(s) 658
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.