RchiOBHm_Chr2g0133711

Brca1-associated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0013575
Physical Location & Seq
Reverse (-)
50613635 .. 50618081
4447 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ50485

Sequence Viewer

Length: 1467 bp
ATGTCGCCGAGCTCGAGCACAGCACGTGCCGGAGCCTCGCCGGTCGAGGCCTTCAGGTCGCCGCCTTCCATAACTCCCGGCGAGCCTAGCGACTTCTCCTGTTCCTCCTCTTCATCCTCCATCTCAAACATCACGCAGGCCTTTCCCTTCTCTTCTGGAAACCCTAGAATTGAAGAGACCAGAGGCATCATGCTCCTCTACCGCGACGATGACGTCGTCTCGTTCTCTCCGTCTAATCTTCCTGTTGAAAGGAAGCCGCTTGTTTGCGTACTTGGAGTGCCGAATCACATGACATATGCTGACTTCTGTAGATTCTGTGGTTCCTTCATTCAGCATATATTGGAGATGCGGATTGTCAGGACCGATGGAATGGAGGATCGTTATAGCATTTTGATTAGGTTTGACAGTCAGGACTCCACAGATCATTTTTACAAGCACTTCAATCGGACCCGGTTCTCATCACTAGAGGTGGAGGTCTGTCGCATACTTTTCACGGTAGATGTTCAATACACTGGTTCAATAGAACATGCACAGGCATCCCTCTGTAGCTCAACTGAGCAGCCATCATGTCCAGTCTGTCTTGAGAGACTAGACCAAGATATGAGTGGAATTCTGACAACTATCTGCAATCATTCTTTCCATTGCTCTTGCATTTCAAAATGGACCGATTCTTCTTGTCCTGTATGTCGATATTGTCAGCAGCAGCCTGAAAAGTCCAACTGTTTCATTTGTCAAACTTCTGAGAATCTCTGGATGTGTATTATCTGTGGCTTTGTTGGTTGTGGGAGGTATAAAGAAGGGCATGCCATAAGACATTGGAAAGAAACACAACATTGCTATTCCCTTGAAGTGGAAACGCAGCGTGTGTGGGATTATGCAGGAGACAATTATGTTCACCGCCTAATTCAATCTAAAACTGATGGAAAATTGGTTGAATTGAATTCACACTGTCGGAATTCGAATGATTTCAAAGGAAGTTGCGAGTGTGTAGATTCTGGATTCAGTGAGGCTCTTTTGAACAGTAAAGTAGAAGCTATTGTTAATGAGTACAACGAACTGCTTGCTACTCAACTTGAGAACCAAAAAATGTATTTTGAATTGCTACTTCAAGAAGTTAAAGAAGGAAGTGAGAGAGAAATGTCTAAAGCTGTAGAAAAGGCTGTGCCAGTAAAGAAAATGCAGGACAAGCTGGATAGATGTGTAAATGAGAAGAAATTTCTGGATGAGCTTAATGATAATCTTTTGAAAAATCGAGAAGCTTGGAAAGCAAAGATACTTGAAGTTGAAGAGAGGGAGAGAAAGGCTTCGAAGCTGAAGGAAGATAAAATAAGCAACTTAGAAGAGCAGCTCAGGAATTTGATGCTGTACCTCGAGACAGGGAATACAGTAGAGTCTTCAAATGAAACAAAGAATGATAATGTCTTGCCAGTATCGATCAACTCATCTCAGCCATCCAATATCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
BRAP2 zf-RING_2 zf-UBP

Protein Analysis

488

Amino Acids

55.5

Weight (kDa)

5.46

Isoelectric Point (pI)

50.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BRAP2 PF07576 80 - 177 3.2e-38 BRCA1-associated protein 2
zf-rbx1 PF12678 180 - 230 4.2e-07 RING-H2 zinc finger domain
zf-RING_2 PF13639 189 - 230 1.8e-08 Ring finger domain
zf-UBP PF02148 241 - 300 9.2e-23 Zn-finger in ubiquitin-hydrolases and other protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013575)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26000 AT2G26000 AT2G26000
fragaria_vesca FvH4_6g29040
malus_domestica MD17G1218900.v1.1
prunus_persica Prupe.3G094400_v2.0.a1 Prupe.3G094400_v2.0.a1
pyrus_communis pycom17g22370
rosa_chinensis RchiOBHm_Chr2g0133711
rosa_laevigata RLG00000019358
rosa_multiflora Rmu_sc0013154.1_g000008
rosa_roxburghii Rroxscaffold_2G00110880
rosa_rugosa Rorug02G0313200
rosa_samantha Rh2AG366600 Rh2BG372300 Rh2CG349900 Rh2DG389200
rosa_wichuraiana Rw2G029830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 212
AatII GACGTC 1 cut(s) 216
AccII CGCG 1 cut(s) 204
AciI CCGC 5 cut(s) 62, 202, 257, 349, 898
AclWI GGATC 1 cut(s) 384
AcsI RAATTY 5 cut(s) 609, 940, 955, 1214, 1354
AcuI CTGAAG 2 cut(s) 37, 1334
AcvI CACGTG 1 cut(s) 26
AcyI GRCGYC 1 cut(s) 213
AfaI GTAC 3 cut(s) 270, 1049, 1367
AfiI CCNNNNNNNGG 1 cut(s) 850
AluBI AGCT 9 cut(s) 12, 549, 1034, 1148, 1189, 1228, 1259, 1312, 1348
AluI AGCT 9 cut(s) 12, 549, 1034, 1148, 1189, 1228, 1259, 1312, 1348
Alw21I GWGCWC 2 cut(s) 14, 20
Alw26I GTCTC 5 cut(s) 170, 223, 580, 876, 1367
AlwI GGATC 1 cut(s) 384
Ama87I CYCGRG 2 cut(s) 13, 1370
AoxI GGCC 2 cut(s) 48, 138
ApeKI GCWGC 5 cut(s) 559, 700, 703, 859, 1345
ApoI RAATTY 5 cut(s) 609, 940, 955, 1214, 1354
Asp700I GAANNNNTTC 2 cut(s) 965, 1303
AspS9I GGNCC 3 cut(s) 360, 447, 663
AsuC2I CCSGG 2 cut(s) 78, 451
AsuHPI GGTGA 1 cut(s) 887
AsuII TTCGAA 2 cut(s) 959, 1307
AvaI CYCGRG 2 cut(s) 13, 1370
AvaII GGWCC 3 cut(s) 360, 447, 663
BanII GRGCYC 1 cut(s) 14
BbrPI CACGTG 1 cut(s) 26
BbsI GAAGAC 1 cut(s) 1386
Bbv12I GWGCWC 2 cut(s) 14, 20
BbvI GCAGC 5 cut(s) 571, 712, 715, 871, 1357
BccI CCATC 5 cut(s) 128, 359, 571, 914, 1459
BcgI CGANNNNNNTGC 4 cut(s) 425, 459, 1043, 1077
BcnI CCSGG 2 cut(s) 78, 451
BcoDI GTCTC 5 cut(s) 170, 223, 580, 876, 1367
BfaI CTAG 4 cut(s) 87, 165, 464, 590
BfmI CTRYAG 3 cut(s) 307, 544, 1149
BisI GCNGC 7 cut(s) 62, 257, 560, 701, 704, 860, 1346
BlsI GCNGC 7 cut(s) 63, 258, 561, 702, 705, 861, 1347
Bme1390I CCNGG 2 cut(s) 78, 451
Bme18I GGWCC 3 cut(s) 360, 447, 663
BmeT110I CYCGRG 2 cut(s) 13, 1370
BmgT120I GGNCC 3 cut(s) 360, 447, 663
BmiI GGNNCC 3 cut(s) 34, 322, 449
BmrFI CCNGG 2 cut(s) 78, 451
BmsI GCATC 4 cut(s) 195, 336, 545, 1350
BpiI GAAGAC 1 cut(s) 1386
Bpu10I CCTNAGC 1 cut(s) 1349
Bpu14I TTCGAA 2 cut(s) 959, 1307
BpuEI CTTGAG 2 cut(s) 602, 1094
BpuMI CCSGG 2 cut(s) 78, 451
Bsa29I ATCGAT 1 cut(s) 1433
BsaAI YACGTR 1 cut(s) 26
BsaHI GRCGYC 1 cut(s) 213
BsaI GGTCTC 1 cut(s) 170
Bsc4I CCNNNNNNNGG 1 cut(s) 850
Bse118I RCCGGY 1 cut(s) 40
Bse1I ACTGG 4 cut(s) 517, 572, 1166, 1427
Bse3DI GCAATG 2 cut(s) 640, 832
BseCI ATCGAT 1 cut(s) 1433
BseGI GGATG 5 cut(s) 113, 536, 759, 1228, 1451
BseLI CCNNNNNNNGG 1 cut(s) 850
BseMI GCAATG 2 cut(s) 640, 832
BseMII CTCAG 4 cut(s) 546, 732, 1363, 1460
BseNI ACTGG 4 cut(s) 517, 572, 1166, 1427
BseRI GAGGAG 2 cut(s) 97, 185
BseXI GCAGC 5 cut(s) 571, 712, 715, 871, 1357
Bsh1236I CGCG 1 cut(s) 204
Bsh1285I CGRYCG 1 cut(s) 45
BshFI GGCC 2 cut(s) 50, 140
BshVI ATCGAT 1 cut(s) 1433
BsiEI CGRYCG 1 cut(s) 45
BsiHKAI GWGCWC 2 cut(s) 14, 20
BsiHKCI CYCGRG 2 cut(s) 13, 1370
BsiSI CCGG 4 cut(s) 30, 41, 78, 451
BslI CCNNNNNNNGG 1 cut(s) 850
BsmAI GTCTC 5 cut(s) 170, 223, 580, 876, 1367
BsmBI CGTCTC 1 cut(s) 223
BsnI GGCC 2 cut(s) 50, 140
Bso31I GGTCTC 1 cut(s) 170
BsoBI CYCGRG 2 cut(s) 13, 1370
Bsp119I TTCGAA 2 cut(s) 959, 1307
Bsp1286I GDGCHC 2 cut(s) 14, 20
Bsp143I GATC 3 cut(s) 376, 421, 1434
BspACI CCGC 5 cut(s) 62, 202, 257, 349, 898
BspANI GGCC 2 cut(s) 50, 140
BspCNI CTCAG 4 cut(s) 547, 733, 1362, 1459
BspDI ATCGAT 1 cut(s) 1433
BspFNI CGCG 1 cut(s) 204
BspLI GGNNCC 3 cut(s) 34, 322, 449
BspPI GGATC 1 cut(s) 384
BspQI GCTCTTC 1 cut(s) 1335
BspT104I TTCGAA 2 cut(s) 959, 1307
BspTNI GGTCTC 1 cut(s) 170
BsrDI GCAATG 2 cut(s) 640, 832
BsrFI RCCGGY 1 cut(s) 40
BsrI ACTGG 4 cut(s) 517, 572, 1166, 1427
BssAI RCCGGY 1 cut(s) 40
BssMI GATC 3 cut(s) 376, 421, 1434
BssNI GRCGYC 1 cut(s) 213
Bst4CI ACNGT 6 cut(s) 407, 496, 722, 950, 1022, 1387
Bst6I CTCTTC 5 cut(s) 115, 157, 168, 1281, 1335
BstACI GRCGYC 1 cut(s) 213
BstBAI YACGTR 1 cut(s) 26
BstBI TTCGAA 2 cut(s) 959, 1307
BstC8I GCNNGC 4 cut(s) 83, 138, 804, 1062
BstDEI CTNAG 5 cut(s) 555, 741, 1336, 1349, 1446
BstF5I GGATG 5 cut(s) 113, 536, 759, 1228, 1451
BstFNI CGCG 1 cut(s) 204
BstKTI GATC 3 cut(s) 379, 424, 1437
BstMAI GTCTC 5 cut(s) 170, 223, 580, 876, 1367
BstMBI GATC 3 cut(s) 376, 421, 1434
BstMCI CGRYCG 1 cut(s) 45
BstMWI GCNNNNNNNGC 3 cut(s) 87, 1186, 1265
BstNSI RCATGY 2 cut(s) 530, 806
BstSCI CCNGG 2 cut(s) 76, 449
BstSFI CTRYAG 3 cut(s) 307, 544, 1149
BstUI CGCG 1 cut(s) 204
BstV1I GCAGC 5 cut(s) 571, 712, 715, 871, 1357
BstV2I GAAGAC 1 cut(s) 1386
Bsu15I ATCGAT 1 cut(s) 1433
BsuRI GGCC 2 cut(s) 50, 140
BsuTUI ATCGAT 1 cut(s) 1433
BtsCI GGATG 5 cut(s) 113, 536, 759, 1228, 1451
BtsIMutI CAGTG 3 cut(s) 510, 946, 1009
Cac8I GCNNGC 4 cut(s) 83, 138, 804, 1062
Cfr10I RCCGGY 1 cut(s) 40
Cfr13I GGNCC 3 cut(s) 360, 447, 663
ClaI ATCGAT 1 cut(s) 1433
Csp6I GTAC 3 cut(s) 269, 1048, 1366
CviAII CATG 5 cut(s) 190, 289, 527, 567, 803
CviQI GTAC 3 cut(s) 269, 1048, 1366
DdeI CTNAG 5 cut(s) 555, 741, 1336, 1349, 1446
DpnI GATC 3 cut(s) 378, 423, 1436
DpnII GATC 3 cut(s) 376, 421, 1434
DrdI GACNNNNNNGTC 1 cut(s) 212
DseDI GACNNNNNNGTC 1 cut(s) 212
Eam1104I CTCTTC 5 cut(s) 115, 157, 168, 1281, 1335
EarI CTCTTC 5 cut(s) 115, 157, 168, 1281, 1335
Ecl136II GAGCTC 1 cut(s) 12
Eco147I AGGCCT 2 cut(s) 50, 140
Eco24I GRGCYC 1 cut(s) 14
Eco31I GGTCTC 1 cut(s) 170
Eco47I GGWCC 3 cut(s) 360, 447, 663
Eco53kI GAGCTC 1 cut(s) 12
Eco57I CTGAAG 2 cut(s) 37, 1334
Eco72I CACGTG 1 cut(s) 26
Eco88I CYCGRG 2 cut(s) 13, 1370
EcoICRI GAGCTC 1 cut(s) 12
EcoRI GAATTC 3 cut(s) 609, 940, 955
EcoT38I GRGCYC 1 cut(s) 14
Esp3I CGTCTC 1 cut(s) 223
FaeI CATG 5 cut(s) 193, 292, 530, 570, 806
FatI CATG 5 cut(s) 189, 288, 526, 566, 802
FauNDI CATATG 1 cut(s) 295
Fnu4HI GCNGC 7 cut(s) 62, 257, 560, 701, 704, 860, 1346
FokI GGATG 5 cut(s) 100, 523, 766, 1235, 1438
FriOI GRGCYC 1 cut(s) 14
Fsp4HI GCNGC 7 cut(s) 62, 257, 560, 701, 704, 860, 1346
FspBI CTAG 4 cut(s) 87, 165, 464, 590
GluI GCNGC 7 cut(s) 62, 257, 560, 701, 704, 860, 1346
HaeIII GGCC 2 cut(s) 50, 140
HapII CCGG 4 cut(s) 30, 41, 78, 451
Hin1I GRCGYC 1 cut(s) 213
Hin1II CATG 5 cut(s) 193, 292, 530, 570, 806
HindIII AAGCTT 1 cut(s) 1257
HinfI GANTC 8 cut(s) 283, 312, 413, 668, 745, 992, 999, 1391
HpaII CCGG 4 cut(s) 30, 41, 78, 451
HphI GGTGA 1 cut(s) 887
Hpy166II GTNNAC 1 cut(s) 895
Hpy188I TCNGA 4 cut(s) 447, 615, 742, 954
Hpy8I GTNNAC 1 cut(s) 895
Hpy99I CGWCG 2 cut(s) 209, 218
HpyAV CCTTC 7 cut(s) 61, 75, 157, 334, 791, 1115, 1309
HpyCH4III ACNGT 6 cut(s) 407, 496, 722, 950, 1022, 1387
HpyCH4IV ACGT 2 cut(s) 25, 213
HpyCH4V TGCA 5 cut(s) 530, 627, 651, 878, 1180
HpyF10VI GCNNNNNNNGC 3 cut(s) 87, 1186, 1265
HpyF3I CTNAG 5 cut(s) 555, 741, 1336, 1349, 1446
HpySE526I ACGT 2 cut(s) 25, 213
Hsp92I GRCGYC 1 cut(s) 213
Hsp92II CATG 5 cut(s) 193, 292, 530, 570, 806
Kzo9I GATC 3 cut(s) 376, 421, 1434
LguI GCTCTTC 1 cut(s) 1335
LmnI GCTCC 2 cut(s) 32, 198
Lsp1109I GCAGC 5 cut(s) 571, 712, 715, 871, 1357
LweI GCATC 4 cut(s) 195, 336, 545, 1350
MaeI CTAG 4 cut(s) 87, 165, 464, 590
MaeII ACGT 2 cut(s) 25, 213
MalI GATC 3 cut(s) 378, 423, 1436
MboI GATC 3 cut(s) 376, 421, 1434
MhlI GDGCHC 2 cut(s) 14, 20
MlyI GAGTC 2 cut(s) 407, 1400
MmeI TCCRAC 2 cut(s) 741, 932
MroXI GAANNNNTTC 2 cut(s) 965, 1303
MseI TTAA 3 cut(s) 1041, 1116, 1230
MspI CCGG 4 cut(s) 30, 41, 78, 451
MspR9I CCNGG 2 cut(s) 78, 451
MvnI CGCG 1 cut(s) 204
MwoI GCNNNNNNNGC 3 cut(s) 87, 1186, 1265
NciI CCSGG 2 cut(s) 78, 451
NdeI CATATG 1 cut(s) 295
NdeII GATC 3 cut(s) 376, 421, 1434
NlaIII CATG 5 cut(s) 193, 292, 530, 570, 806
NlaIV GGNNCC 3 cut(s) 34, 322, 449
NmeAIII GCCGAG 1 cut(s) 33
NspI RCATGY 2 cut(s) 530, 806
NspV TTCGAA 2 cut(s) 959, 1307
PaeI GCATGC 1 cut(s) 806
PaeR7I CTCGAG 2 cut(s) 13, 1370
PceI AGGCCT 2 cut(s) 50, 140
PciSI GCTCTTC 1 cut(s) 1335
PcsI WCGNNNNNNNCGW 3 cut(s) 11, 213, 227
PdmI GAANNNNTTC 2 cut(s) 965, 1303
PfeI GAWTC 6 cut(s) 283, 312, 668, 745, 992, 999
PflFI GACNNNGTC 1 cut(s) 215
PkrI GCNGC 7 cut(s) 63, 258, 561, 702, 705, 861, 1347
PleI GAGTC 2 cut(s) 407, 1399
PmaCI CACGTG 1 cut(s) 26
PmlI CACGTG 1 cut(s) 26
PpsI GAGTC 2 cut(s) 407, 1399
Ppu21I YACGTR 1 cut(s) 26
Psp124BI GAGCTC 1 cut(s) 14
PspCI CACGTG 1 cut(s) 26
PspN4I GGNNCC 3 cut(s) 34, 322, 449
PspPI GGNCC 3 cut(s) 360, 447, 663
PspXI VCTCGAGB 1 cut(s) 13
PsyI GACNNNGTC 1 cut(s) 215
RsaI GTAC 3 cut(s) 270, 1049, 1367
RsaNI GTAC 3 cut(s) 269, 1048, 1366
SacI GAGCTC 1 cut(s) 14
SapI GCTCTTC 1 cut(s) 1335
SaqAI TTAA 3 cut(s) 1041, 1116, 1230
SatI GCNGC 7 cut(s) 62, 257, 560, 701, 704, 860, 1346
Sau3AI GATC 3 cut(s) 376, 421, 1434
Sau96I GGNCC 3 cut(s) 360, 447, 663
SchI GAGTC 2 cut(s) 407, 1400
ScrFI CCNGG 2 cut(s) 78, 451
SduI GDGCHC 2 cut(s) 14, 20
SfaNI GCATC 4 cut(s) 195, 336, 545, 1350
SfcI CTRYAG 3 cut(s) 307, 544, 1149
Sfr274I CTCGAG 2 cut(s) 13, 1370
SfuI TTCGAA 2 cut(s) 959, 1307
SinI GGWCC 3 cut(s) 360, 447, 663
SlaI CTCGAG 2 cut(s) 13, 1370
SmlI CTYRAG 4 cut(s) 13, 581, 1073, 1370
SmoI CTYRAG 4 cut(s) 13, 581, 1073, 1370
SphI GCATGC 1 cut(s) 806
SseBI AGGCCT 2 cut(s) 50, 140
SsiI CCGC 5 cut(s) 62, 202, 257, 349, 898
SspMI CTAG 4 cut(s) 87, 165, 464, 590
SstI GAGCTC 1 cut(s) 14
StuI AGGCCT 2 cut(s) 50, 140
StyD4I CCNGG 2 cut(s) 76, 449
TaaI ACNGT 6 cut(s) 407, 496, 722, 950, 1022, 1387
TaiI ACGT 2 cut(s) 28, 216
TaqI TCGA 8 cut(s) 14, 45, 688, 959, 1252, 1307, 1371, 1433
TaqII GACCGA 2 cut(s) 377, 680
TatI WGTACW 1 cut(s) 1047
TauI GCSGC 2 cut(s) 64, 259
TfiI GAWTC 6 cut(s) 283, 312, 668, 745, 992, 999
Tru1I TTAA 3 cut(s) 1041, 1116, 1230
Tru9I TTAA 3 cut(s) 1041, 1116, 1230
TscAI CASTG 3 cut(s) 517, 953, 1009
TseI GCWGC 5 cut(s) 559, 700, 703, 859, 1345
TspDTI ATGAA 4 cut(s) 102, 316, 715, 1416
TspGWI ACGGA 1 cut(s) 219
TspRI CASTG 3 cut(s) 517, 953, 1009
Tth111I GACNNNGTC 1 cut(s) 215
VpaK11BI GGWCC 3 cut(s) 360, 447, 663
XapI RAATTY 5 cut(s) 609, 940, 955, 1214, 1354
XceI RCATGY 2 cut(s) 530, 806
XcmI CCANNNNNNNNNTGG 1 cut(s) 602
XhoI CTCGAG 2 cut(s) 13, 1370
XmnI GAANNNNTTC 2 cut(s) 965, 1303
XspI CTAG 4 cut(s) 87, 165, 464, 590
ZraI GACGTC 1 cut(s) 214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.