RLG00000019358

Brca1-associated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0013575
Physical Location & Seq
Reverse (-)
50771227 .. 50776024
4798 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019358

Sequence Viewer

Length: 1551 bp
ATGTCGCCGAGCTCGAGCACAGCACGTGCCGGAGCCTCGCCGGTCGAGGCCTTCAGGTCGCCGCCTTCGTTAACTCCCGGCGAGCCTAGCGACTTCTCCTGTTCCTCCTCTTCATCCTCCATCTCAAACATCACGCAGGCCTTTCCTTTCTCTTCTGGAAACCCTAGAATTGAAGAGACCAGAGGCATCATGCTCCTCTACCGCGACGATGACGTCGTCTCGTTCTCTCCGTCTAATCTTCCTGTTGACAGGAAGCCGCTTGTTTGCGTACTTGGAGTGCCGAATCACATGACATATGCTGACTTCTGTAGATTCTGTGGTTCTTTCATTCAGCATATATTGGAGATGCGGATTGTCAGGACCGATGGAATGGAGGATCGTTATAGCATTTTGATTAGGTTTGATAGTCAGGACTCCACAGATCATTTTTACAAGCACGTCAATCGGACCCGGTTCTCATCACTAGAGGTGGAGGTCTGTCGCATACTTTACACAGTAGATGTTCAATACACTGGTTCAATAGAACATGCACAGGCATCCCTCTGTAGCTCAACCGAGCAGCCATCATGTCCAGTCTGTCTTGAGAGACTAGACCAAGATATGAGTGGAATTCTCACAACTATCTGCAATCATTCTTTCCATTGCTCTTGCATTTCAAAATGGACCGATTCTTCTTGTCCTGTATGTCGATATTGTCAGCAGCAGCCTGAAAAGTCCAACTGTTTCATTTGTCAAACTTCTGAGAATCTCTGGATGTGTATTATCTGTGGCTTTGTTGGTTGTGGGAGGTATAAAGAAGGGCATGCCATAAGACATTGGAAAGAAACACAACATTGCTATTCCCTTGAAGTGGAAACACAGCGTGTGTGGGATTATGCAGGAGACAATTATGTTCACCGCCTAATTCAATCTAAAACTGATGGAAAATTGGTTGAATTGAATTCACACTGTCGGAATGCGAATGATTTCAAAGGAAGTTGCGAGTGTGTAGATTCTGGATTCAGTGAGGCTCTTTTGAACAGTAAAGTAGAAGCTATTGTTAATGAGTACAACGAACTGCTTGCTACTCAACTTGAGAACCAAAAAATGTATTTTGAGTCGCTACTTCAAGAAGTTAAAGAAGGAAGTGAGAGAGAAATTTCTAAATCTGTAGAAAAGGCTGTGCCAGTAAAGAAAATGCAGGACAAGCTGGATCGATGTGTAAAGGAGAAGAAATTTCTGGATGAGCTTAATGATAATCTTTTGAAAAATCGAGAAGCTTGGAAAGCAAAGATACTTGAAGTTGAAGAGAGGGAGAGAAAGGCTTCAAAACTGAAGGATGATAAAATAAGCAACTTAGAAGAGCAGCTCAGGAATTTGATGCTGCACCTCGAGACAGGGAATACAGTAGAGTCATCAAATGAAACAAAGAATGGTGGTTTTGGCTCAAGATCGACGAAAATTCTCTGGGATGCATGCGGTGCAGTTATGGTGGGTCTCGGTTTGCAAATCGATGGCGTTGCAGATGTTGAGCGCTGGGTCGTGGCGGTTTCGACTTCGGCGACGGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
BRAP2 zf-RING_2 zf-UBP

Protein Analysis

517

Amino Acids

58.12

Weight (kDa)

5.52

Isoelectric Point (pI)

48.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BRAP2 PF07576 80 - 177 5.5e-37 BRCA1-associated protein 2
zf-rbx1 PF12678 180 - 230 4.5e-07 RING-H2 zinc finger domain
zf-RING_2 PF13639 189 - 230 2e-08 Ring finger domain
zf-UBP PF02148 241 - 300 1e-22 Zn-finger in ubiquitin-hydrolases and other protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013575)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26000 AT2G26000 AT2G26000
fragaria_vesca FvH4_6g29040
malus_domestica MD17G1218900.v1.1
prunus_persica Prupe.3G094400_v2.0.a1 Prupe.3G094400_v2.0.a1
pyrus_communis pycom17g22370
rosa_chinensis RchiOBHm_Chr2g0133711
rosa_laevigata RLG00000019358
rosa_multiflora Rmu_sc0013154.1_g000008
rosa_roxburghii Rroxscaffold_2G00110880
rosa_rugosa Rorug02G0313200
rosa_samantha Rh2AG366600 Rh2BG372300 Rh2CG349900 Rh2DG389200
rosa_wichuraiana Rw2G029830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 212
AatII GACGTC 1 cut(s) 216
AccII CGCG 1 cut(s) 204
AciI CCGC 7 cut(s) 62, 202, 257, 349, 898, 1458, 1526
AclWI GGATC 2 cut(s) 384, 1200
AcsI RAATTY 6 cut(s) 609, 940, 1137, 1214, 1354, 1440
AcuI CTGAAG 2 cut(s) 37, 1334
AcvI CACGTG 1 cut(s) 26
AcyI GRCGYC 1 cut(s) 213
AdeI CACNNNGTG 1 cut(s) 863
AfaI GTAC 2 cut(s) 270, 1049
AfeI AGCGCT 1 cut(s) 1514
AfiI CCNNNNNNNGG 1 cut(s) 850
AjiI CACGTC 1 cut(s) 439
AluBI AGCT 7 cut(s) 12, 549, 1034, 1189, 1228, 1259, 1348
AluI AGCT 7 cut(s) 12, 549, 1034, 1189, 1228, 1259, 1348
Alw21I GWGCWC 2 cut(s) 14, 20
Alw26I GTCTC 6 cut(s) 170, 223, 580, 876, 1367, 1481
AlwI GGATC 2 cut(s) 384, 1200
Ama87I CYCGRG 2 cut(s) 13, 1370
Aor51HI AGCGCT 1 cut(s) 1514
AoxI GGCC 2 cut(s) 48, 138
ApeKI GCWGC 5 cut(s) 559, 700, 703, 1345, 1363
ApoI RAATTY 6 cut(s) 609, 940, 1137, 1214, 1354, 1440
Asp700I GAANNNNTTC 2 cut(s) 965, 1303
AspLEI GCGC 1 cut(s) 1515
AspS9I GGNCC 3 cut(s) 360, 447, 663
AsuC2I CCSGG 2 cut(s) 78, 451
AsuHPI GGTGA 1 cut(s) 887
AvaI CYCGRG 2 cut(s) 13, 1370
AvaII GGWCC 3 cut(s) 360, 447, 663
BanII GRGCYC 1 cut(s) 14
BbrPI CACGTG 1 cut(s) 26
Bbv12I GWGCWC 2 cut(s) 14, 20
BbvI GCAGC 5 cut(s) 571, 712, 715, 1350, 1357
BccI CCATC 5 cut(s) 128, 359, 571, 914, 1487
BcgI CGANNNNNNTGC 6 cut(s) 425, 459, 1043, 1077, 1481, 1515
BcnI CCSGG 2 cut(s) 78, 451
BcoDI GTCTC 6 cut(s) 170, 223, 580, 876, 1367, 1481
BfaI CTAG 4 cut(s) 87, 165, 464, 590
BfmI CTRYAG 3 cut(s) 307, 544, 1149
BfoI RGCGCY 1 cut(s) 1516
BisI GCNGC 7 cut(s) 62, 257, 560, 701, 704, 1346, 1364
BlsI GCNGC 7 cut(s) 63, 258, 561, 702, 705, 1347, 1365
Bme1390I CCNGG 2 cut(s) 78, 451
Bme18I GGWCC 3 cut(s) 360, 447, 663
BmeT110I CYCGRG 2 cut(s) 13, 1370
BmgBI CACGTC 1 cut(s) 439
BmgT120I GGNCC 3 cut(s) 360, 447, 663
BmiI GGNNCC 2 cut(s) 34, 449
BmrFI CCNGG 2 cut(s) 78, 451
BmsI GCATC 5 cut(s) 195, 336, 545, 1350, 1441
Bpu10I CCTNAGC 1 cut(s) 1349
BpuEI CTTGAG 3 cut(s) 602, 1094, 1411
BpuMI CCSGG 2 cut(s) 78, 451
Bsa29I ATCGAT 2 cut(s) 1195, 1491
BsaAI YACGTR 1 cut(s) 26
BsaHI GRCGYC 1 cut(s) 213
BsaI GGTCTC 2 cut(s) 170, 1481
Bsc4I CCNNNNNNNGG 1 cut(s) 850
Bse118I RCCGGY 1 cut(s) 40
Bse1I ACTGG 3 cut(s) 517, 572, 1166
Bse3DI GCAATG 2 cut(s) 640, 832
BseCI ATCGAT 2 cut(s) 1195, 1491
BseGI GGATG 6 cut(s) 113, 536, 759, 1228, 1324, 1456
BseLI CCNNNNNNNGG 1 cut(s) 850
BseMI GCAATG 2 cut(s) 640, 832
BseMII CTCAG 2 cut(s) 732, 1363
BseNI ACTGG 3 cut(s) 517, 572, 1166
BseRI GAGGAG 2 cut(s) 97, 185
BseXI GCAGC 5 cut(s) 571, 712, 715, 1350, 1357
BseYI CCCAGC 1 cut(s) 1515
BsgI GTGCAG 2 cut(s) 1349, 1482
Bsh1236I CGCG 1 cut(s) 204
Bsh1285I CGRYCG 1 cut(s) 45
BshFI GGCC 2 cut(s) 50, 140
BshVI ATCGAT 2 cut(s) 1195, 1491
BsiEI CGRYCG 1 cut(s) 45
BsiHKAI GWGCWC 2 cut(s) 14, 20
BsiHKCI CYCGRG 2 cut(s) 13, 1370
BsiSI CCGG 4 cut(s) 30, 41, 78, 451
BslI CCNNNNNNNGG 1 cut(s) 850
BsmAI GTCTC 6 cut(s) 170, 223, 580, 876, 1367, 1481
BsmBI CGTCTC 1 cut(s) 223
BsmI GAATGC 1 cut(s) 961
BsnI GGCC 2 cut(s) 50, 140
Bso31I GGTCTC 2 cut(s) 170, 1481
BsoBI CYCGRG 2 cut(s) 13, 1370
Bsp1286I GDGCHC 2 cut(s) 14, 20
Bsp143I GATC 4 cut(s) 376, 421, 1192, 1430
BspACI CCGC 7 cut(s) 62, 202, 257, 349, 898, 1458, 1526
BspANI GGCC 2 cut(s) 50, 140
BspCNI CTCAG 2 cut(s) 733, 1362
BspDI ATCGAT 2 cut(s) 1195, 1491
BspFNI CGCG 1 cut(s) 204
BspLI GGNNCC 2 cut(s) 34, 449
BspPI GGATC 2 cut(s) 384, 1200
BspQI GCTCTTC 1 cut(s) 1335
BspTNI GGTCTC 2 cut(s) 170, 1481
BsrDI GCAATG 2 cut(s) 640, 832
BsrFI RCCGGY 1 cut(s) 40
BsrI ACTGG 3 cut(s) 517, 572, 1166
BssAI RCCGGY 1 cut(s) 40
BssMI GATC 4 cut(s) 376, 421, 1192, 1430
BssNI GRCGYC 1 cut(s) 213
Bst4CI ACNGT 5 cut(s) 496, 722, 950, 1022, 1387
Bst6I CTCTTC 5 cut(s) 115, 157, 168, 1281, 1335
BstACI GRCGYC 1 cut(s) 213
BstAPI GCANNNNNTGC 1 cut(s) 1460
BstBAI YACGTR 1 cut(s) 26
BstC8I GCNNGC 5 cut(s) 83, 138, 804, 1062, 1456
BstDEI CTNAG 3 cut(s) 741, 1336, 1349
BstF5I GGATG 6 cut(s) 113, 536, 759, 1228, 1324, 1456
BstFNI CGCG 1 cut(s) 204
BstH2I RGCGCY 1 cut(s) 1516
BstHHI GCGC 1 cut(s) 1515
BstKTI GATC 4 cut(s) 379, 424, 1195, 1433
BstMAI GTCTC 6 cut(s) 170, 223, 580, 876, 1367, 1481
BstMBI GATC 4 cut(s) 376, 421, 1192, 1430
BstMCI CGRYCG 1 cut(s) 45
BstMWI GCNNNNNNNGC 4 cut(s) 87, 1186, 1265, 1460
BstNSI RCATGY 3 cut(s) 530, 806, 1458
BstSCI CCNGG 2 cut(s) 76, 449
BstSFI CTRYAG 3 cut(s) 307, 544, 1149
BstUI CGCG 1 cut(s) 204
BstV1I GCAGC 5 cut(s) 571, 712, 715, 1350, 1357
Bsu15I ATCGAT 2 cut(s) 1195, 1491
BsuRI GGCC 2 cut(s) 50, 140
BsuTUI ATCGAT 2 cut(s) 1195, 1491
BtrI CACGTC 1 cut(s) 439
BtsCI GGATG 6 cut(s) 113, 536, 759, 1228, 1324, 1456
BtsIMutI CAGTG 3 cut(s) 510, 946, 1009
Cac8I GCNNGC 5 cut(s) 83, 138, 804, 1062, 1456
CfoI GCGC 1 cut(s) 1515
Cfr10I RCCGGY 1 cut(s) 40
Cfr13I GGNCC 3 cut(s) 360, 447, 663
ClaI ATCGAT 2 cut(s) 1195, 1491
Csp6I GTAC 2 cut(s) 269, 1048
CviAII CATG 6 cut(s) 190, 289, 527, 567, 803, 1455
CviQI GTAC 2 cut(s) 269, 1048
DdeI CTNAG 3 cut(s) 741, 1336, 1349
DpnI GATC 4 cut(s) 378, 423, 1194, 1432
DpnII GATC 4 cut(s) 376, 421, 1192, 1430
DraIII CACNNNGTG 1 cut(s) 863
DrdI GACNNNNNNGTC 1 cut(s) 212
DseDI GACNNNNNNGTC 1 cut(s) 212
Eam1104I CTCTTC 5 cut(s) 115, 157, 168, 1281, 1335
EarI CTCTTC 5 cut(s) 115, 157, 168, 1281, 1335
Ecl136II GAGCTC 1 cut(s) 12
Eco147I AGGCCT 2 cut(s) 50, 140
Eco24I GRGCYC 1 cut(s) 14
Eco31I GGTCTC 2 cut(s) 170, 1481
Eco47I GGWCC 3 cut(s) 360, 447, 663
Eco47III AGCGCT 1 cut(s) 1514
Eco53kI GAGCTC 1 cut(s) 12
Eco57I CTGAAG 2 cut(s) 37, 1334
Eco72I CACGTG 1 cut(s) 26
Eco88I CYCGRG 2 cut(s) 13, 1370
EcoICRI GAGCTC 1 cut(s) 12
EcoRI GAATTC 2 cut(s) 609, 940
EcoT22I ATGCAT 1 cut(s) 1456
EcoT38I GRGCYC 1 cut(s) 14
Esp3I CGTCTC 1 cut(s) 223
FaeI CATG 6 cut(s) 193, 292, 530, 570, 806, 1458
FatI CATG 6 cut(s) 189, 288, 526, 566, 802, 1454
FauNDI CATATG 1 cut(s) 295
Fnu4HI GCNGC 7 cut(s) 62, 257, 560, 701, 704, 1346, 1364
FokI GGATG 6 cut(s) 100, 523, 766, 1235, 1331, 1463
FriOI GRGCYC 1 cut(s) 14
Fsp4HI GCNGC 7 cut(s) 62, 257, 560, 701, 704, 1346, 1364
FspBI CTAG 4 cut(s) 87, 165, 464, 590
GlaI GCGC 1 cut(s) 1514
GluI GCNGC 7 cut(s) 62, 257, 560, 701, 704, 1346, 1364
GsaI CCCAGC 1 cut(s) 1519
HaeII RGCGCY 1 cut(s) 1516
HaeIII GGCC 2 cut(s) 50, 140
HapII CCGG 4 cut(s) 30, 41, 78, 451
HhaI GCGC 1 cut(s) 1515
Hin1I GRCGYC 1 cut(s) 213
Hin1II CATG 6 cut(s) 193, 292, 530, 570, 806, 1458
Hin6I GCGC 1 cut(s) 1513
HinP1I GCGC 1 cut(s) 1513
HincII GTYRAC 2 cut(s) 72, 247
HindII GTYRAC 2 cut(s) 72, 247
HindIII AAGCTT 1 cut(s) 1257
HinfI GANTC 9 cut(s) 283, 312, 413, 668, 745, 992, 999, 1097, 1391
HpaI GTTAAC 1 cut(s) 72
HpaII CCGG 4 cut(s) 30, 41, 78, 451
HphI GGTGA 1 cut(s) 887
Hpy166II GTNNAC 3 cut(s) 72, 247, 895
Hpy188I TCNGA 3 cut(s) 447, 742, 954
Hpy8I GTNNAC 3 cut(s) 72, 247, 895
Hpy99I CGWCG 4 cut(s) 209, 218, 1438, 1546
HpyAV CCTTC 5 cut(s) 61, 75, 791, 1115, 1309
HpyCH4III ACNGT 5 cut(s) 496, 722, 950, 1022, 1387
HpyCH4IV ACGT 3 cut(s) 25, 213, 438
HpyF10VI GCNNNNNNNGC 4 cut(s) 87, 1186, 1265, 1460
HpyF3I CTNAG 3 cut(s) 741, 1336, 1349
HpySE526I ACGT 3 cut(s) 25, 213, 438
Hsp92I GRCGYC 1 cut(s) 213
Hsp92II CATG 6 cut(s) 193, 292, 530, 570, 806, 1458
HspAI GCGC 1 cut(s) 1513
KspAI GTTAAC 1 cut(s) 72
Kzo9I GATC 4 cut(s) 376, 421, 1192, 1430
LguI GCTCTTC 1 cut(s) 1335
LmnI GCTCC 2 cut(s) 32, 198
Lsp1109I GCAGC 5 cut(s) 571, 712, 715, 1350, 1357
LweI GCATC 5 cut(s) 195, 336, 545, 1350, 1441
MaeI CTAG 4 cut(s) 87, 165, 464, 590
MaeII ACGT 3 cut(s) 25, 213, 438
MalI GATC 4 cut(s) 378, 423, 1194, 1432
MboI GATC 4 cut(s) 376, 421, 1192, 1430
MboII GAAGA 8 cut(s) 102, 144, 185, 230, 663, 1222, 1298, 1352
MhlI GDGCHC 2 cut(s) 14, 20
MlyI GAGTC 3 cut(s) 407, 1106, 1400
MmeI TCCRAC 2 cut(s) 741, 932
Mph1103I ATGCAT 1 cut(s) 1456
MroXI GAANNNNTTC 2 cut(s) 965, 1303
MseI TTAA 4 cut(s) 71, 1041, 1116, 1230
MspI CCGG 4 cut(s) 30, 41, 78, 451
MspR9I CCNGG 2 cut(s) 78, 451
Mva1269I GAATGC 1 cut(s) 961
MvnI CGCG 1 cut(s) 204
MwoI GCNNNNNNNGC 4 cut(s) 87, 1186, 1265, 1460
NciI CCSGG 2 cut(s) 78, 451
NdeI CATATG 1 cut(s) 295
NdeII GATC 4 cut(s) 376, 421, 1192, 1430
NlaIII CATG 6 cut(s) 193, 292, 530, 570, 806, 1458
NlaIV GGNNCC 2 cut(s) 34, 449
NmeAIII GCCGAG 1 cut(s) 33
NsiI ATGCAT 1 cut(s) 1456
NspI RCATGY 3 cut(s) 530, 806, 1458
PaeI GCATGC 2 cut(s) 806, 1458
PaeR7I CTCGAG 2 cut(s) 13, 1370
PceI AGGCCT 2 cut(s) 50, 140
PciSI GCTCTTC 1 cut(s) 1335
PcsI WCGNNNNNNNCGW 6 cut(s) 11, 65, 213, 227, 1538, 1544
PctI GAATGC 1 cut(s) 961
PdmI GAANNNNTTC 2 cut(s) 965, 1303
PfeI GAWTC 6 cut(s) 283, 312, 668, 745, 992, 999
PflFI GACNNNGTC 1 cut(s) 215
PkrI GCNGC 7 cut(s) 63, 258, 561, 702, 705, 1347, 1365
PleI GAGTC 3 cut(s) 407, 1105, 1399
PmaCI CACGTG 1 cut(s) 26
PmlI CACGTG 1 cut(s) 26
PpsI GAGTC 3 cut(s) 407, 1105, 1399
Ppu21I YACGTR 1 cut(s) 26
Psp124BI GAGCTC 1 cut(s) 14
PspCI CACGTG 1 cut(s) 26
PspFI CCCAGC 1 cut(s) 1515
PspN4I GGNNCC 2 cut(s) 34, 449
PspPI GGNCC 3 cut(s) 360, 447, 663
PspXI VCTCGAGB 1 cut(s) 13
PsyI GACNNNGTC 1 cut(s) 215
RsaI GTAC 2 cut(s) 270, 1049
RsaNI GTAC 2 cut(s) 269, 1048
SacI GAGCTC 1 cut(s) 14
SapI GCTCTTC 1 cut(s) 1335
SaqAI TTAA 4 cut(s) 71, 1041, 1116, 1230
SatI GCNGC 7 cut(s) 62, 257, 560, 701, 704, 1346, 1364
Sau3AI GATC 4 cut(s) 376, 421, 1192, 1430
Sau96I GGNCC 3 cut(s) 360, 447, 663
SchI GAGTC 3 cut(s) 407, 1106, 1400
ScrFI CCNGG 2 cut(s) 78, 451
SduI GDGCHC 2 cut(s) 14, 20
SfaNI GCATC 5 cut(s) 195, 336, 545, 1350, 1441
SfcI CTRYAG 3 cut(s) 307, 544, 1149
Sfr274I CTCGAG 2 cut(s) 13, 1370
SinI GGWCC 3 cut(s) 360, 447, 663
SlaI CTCGAG 2 cut(s) 13, 1370
SmlI CTYRAG 5 cut(s) 13, 581, 1073, 1370, 1426
SmoI CTYRAG 5 cut(s) 13, 581, 1073, 1370, 1426
SphI GCATGC 2 cut(s) 806, 1458
SseBI AGGCCT 2 cut(s) 50, 140
SsiI CCGC 7 cut(s) 62, 202, 257, 349, 898, 1458, 1526
SspMI CTAG 4 cut(s) 87, 165, 464, 590
SstI GAGCTC 1 cut(s) 14
StuI AGGCCT 2 cut(s) 50, 140
StyD4I CCNGG 2 cut(s) 76, 449
TaaI ACNGT 5 cut(s) 496, 722, 950, 1022, 1387
TaiI ACGT 3 cut(s) 28, 216, 441
TaqI TCGA 9 cut(s) 14, 45, 688, 1195, 1252, 1371, 1433, 1491, 1532
TaqII GACCGA 2 cut(s) 377, 680
TatI WGTACW 1 cut(s) 1047
TauI GCSGC 2 cut(s) 64, 259
TfiI GAWTC 6 cut(s) 283, 312, 668, 745, 992, 999
Tru1I TTAA 4 cut(s) 71, 1041, 1116, 1230
Tru9I TTAA 4 cut(s) 71, 1041, 1116, 1230
TscAI CASTG 3 cut(s) 517, 953, 1009
TseI GCWGC 5 cut(s) 559, 700, 703, 1345, 1363
TspDTI ATGAA 4 cut(s) 102, 316, 715, 1416
TspGWI ACGGA 1 cut(s) 219
TspRI CASTG 3 cut(s) 517, 953, 1009
Tth111I GACNNNGTC 1 cut(s) 215
VpaK11BI GGWCC 3 cut(s) 360, 447, 663
XapI RAATTY 6 cut(s) 609, 940, 1137, 1214, 1354, 1440
XceI RCATGY 3 cut(s) 530, 806, 1458
XcmI CCANNNNNNNNNTGG 1 cut(s) 602
XhoI CTCGAG 2 cut(s) 13, 1370
XmnI GAANNNNTTC 2 cut(s) 965, 1303
XspI CTAG 4 cut(s) 87, 165, 464, 590
ZraI GACGTC 1 cut(s) 214
Zsp2I ATGCAT 1 cut(s) 1456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.