Rh2DG389200

Brca1-associated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0013575
Physical Location & Seq
Reverse (-)
56380245 .. 56384874
4630 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG389200.1

Sequence Viewer

Length: 1149 bp
ATGTCGCCGAGCTCGAGCACAGCACGTGCCGGAGCCTCGCCGGTCGAGGCCTTCAGGTCGCCGCCTTCCATAACTCCCGGCGAGCCTAGCGACTTCTCCTGTTCCTCCTCTTCATCCTCCATCTCAAACATCACGCAGGCCTTTCCCTTCTCTTCTGGAAACCCTAGAATTGAAGAGACCAGAGGCATCATGCTCCTCTACCGCGACGATGACGTCGTCTCGTTCTCTCCGTCTAATCTTCCTGTTGAAAGGAAGCCGCTTGTTTGCGTACTTGGAGTGCCGAATCACATGACATATGCTGACTTCTGTAGATTCTGTGGTTCCTTCATTCAGCATATATTGGAGATGCGGATTGTCAGGACCGATGGAATGGAGGATCGTTATAGCATTTTGATTAGGTTTGACAGTCAGGACTCCACAGATCATTTTTACAAGCACTTCAATCGGACCCGGTTCTCATCACTAGAGGTGGAGGTCTGTCGCATACTTTTCACGGTAGATGTTCAATACACTGGTTCAATAGAACATGCACAGGCATCCCTCTGTAGCTCAACTGAGCAGCCATCATGTCCAGTCTGTCTTGAGAGACTAGACCAAGATATGAGTGGAATTCTGACGACTATCTGCAATCATTCTTTCCATTGCTCTTGCATTTCAAAATGGACCGATTCTTCTTGTCCTGTATGTCGATATTGTCAGCAGCAGCCTGAAAAGTCCAACTGTTTCATTTGTCAAACTTCTGAGAATCTCTGGATGTGTATTATCTGTGGCTTTGTTGGTTGTGGGAGGTATAAAGAAGGGCATGCCATAAGACATTGGAAAGAAACACAACATTGCTATTCCCTTGAAGTGGAAACGCAGCGTGTGTGGGATTATGCAGGAGACAATTATGTTCACCGCCTAATTCAATCTAAAACTGATGGAAAATTGGTTGAATTGAATTCACACTGTCGGAATTCGAATGATTTCAAAGGAAGTTGCGAGTGTGTAGATTCTGGATTCAGTGAGGCTCTTTTGAACAGTAAAGTAGAAGCTATTGTTAATGAGTACAACGAACTGCTTGCTACTCAACTTGAGAACCAAAAAATGGTAATCACTGCAACTGGTTTCTCTTTAAACTTATTTAGTTTCACGCTGCATTTAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
BRAP2 zf-RING_2 zf-UBP

Protein Analysis

382

Amino Acids

43.04

Weight (kDa)

5.54

Isoelectric Point (pI)

49.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BRAP2 PF07576 80 - 177 2.1e-38 BRCA1-associated protein 2
zf-rbx1 PF12678 180 - 230 3e-07 RING-H2 zinc finger domain
zf-RING_2 PF13639 189 - 230 1.3e-08 Ring finger domain
zf-UBP PF02148 241 - 300 6.4e-23 Zn-finger in ubiquitin-hydrolases and other protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013575)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26000 AT2G26000 AT2G26000
fragaria_vesca FvH4_6g29040
malus_domestica MD17G1218900.v1.1
prunus_persica Prupe.3G094400_v2.0.a1 Prupe.3G094400_v2.0.a1
pyrus_communis pycom17g22370
rosa_chinensis RchiOBHm_Chr2g0133711
rosa_laevigata RLG00000019358
rosa_multiflora Rmu_sc0013154.1_g000008
rosa_roxburghii Rroxscaffold_2G00110880
rosa_rugosa Rorug02G0313200
rosa_samantha Rh2AG366600 Rh2BG372300 Rh2CG349900 Rh2DG389200
rosa_wichuraiana Rw2G029830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 212
AatII GACGTC 1 cut(s) 216
AccB7I CCANNNNNTGG 1 cut(s) 1087
AccII CGCG 1 cut(s) 204
AciI CCGC 5 cut(s) 62, 202, 257, 349, 898
AclWI GGATC 1 cut(s) 384
AcsI RAATTY 3 cut(s) 609, 940, 955
AcuI CTGAAG 1 cut(s) 37
AcvI CACGTG 1 cut(s) 26
AcyI GRCGYC 1 cut(s) 213
AfaI GTAC 2 cut(s) 270, 1049
AfiI CCNNNNNNNGG 2 cut(s) 850, 1087
AluBI AGCT 3 cut(s) 12, 549, 1034
AluI AGCT 3 cut(s) 12, 549, 1034
Alw21I GWGCWC 2 cut(s) 14, 20
Alw26I GTCTC 4 cut(s) 170, 223, 580, 876
AlwI GGATC 1 cut(s) 384
Ama87I CYCGRG 1 cut(s) 13
AoxI GGCC 2 cut(s) 48, 138
ApeKI GCWGC 5 cut(s) 559, 700, 703, 859, 1135
ApoI RAATTY 3 cut(s) 609, 940, 955
Asp700I GAANNNNTTC 1 cut(s) 965
AspS9I GGNCC 3 cut(s) 360, 447, 663
AsuC2I CCSGG 2 cut(s) 78, 451
AsuHPI GGTGA 1 cut(s) 887
AsuII TTCGAA 1 cut(s) 959
AvaI CYCGRG 1 cut(s) 13
AvaII GGWCC 3 cut(s) 360, 447, 663
BanII GRGCYC 1 cut(s) 14
BbrPI CACGTG 1 cut(s) 26
Bbv12I GWGCWC 2 cut(s) 14, 20
BbvI GCAGC 5 cut(s) 571, 712, 715, 871, 1122
BccI CCATC 4 cut(s) 128, 359, 571, 914
BcgI CGANNNNNNTGC 4 cut(s) 425, 459, 1043, 1077
BcnI CCSGG 2 cut(s) 78, 451
BcoDI GTCTC 4 cut(s) 170, 223, 580, 876
BfaI CTAG 4 cut(s) 87, 165, 464, 590
BfmI CTRYAG 2 cut(s) 307, 544
BisI GCNGC 7 cut(s) 62, 257, 560, 701, 704, 860, 1136
BlsI GCNGC 7 cut(s) 63, 258, 561, 702, 705, 861, 1137
Bme1390I CCNGG 2 cut(s) 78, 451
Bme18I GGWCC 3 cut(s) 360, 447, 663
BmeT110I CYCGRG 1 cut(s) 13
BmgT120I GGNCC 3 cut(s) 360, 447, 663
BmiI GGNNCC 3 cut(s) 34, 322, 449
BmrFI CCNGG 2 cut(s) 78, 451
BmsI GCATC 3 cut(s) 195, 336, 545
Bpu14I TTCGAA 1 cut(s) 959
BpuEI CTTGAG 2 cut(s) 602, 1094
BpuMI CCSGG 2 cut(s) 78, 451
BsaAI YACGTR 1 cut(s) 26
BsaHI GRCGYC 1 cut(s) 213
BsaI GGTCTC 1 cut(s) 170
Bsc4I CCNNNNNNNGG 2 cut(s) 850, 1087
Bse118I RCCGGY 1 cut(s) 40
Bse1I ACTGG 3 cut(s) 517, 572, 1108
Bse3DI GCAATG 2 cut(s) 640, 832
BseGI GGATG 3 cut(s) 113, 536, 759
BseLI CCNNNNNNNGG 2 cut(s) 850, 1087
BseMI GCAATG 2 cut(s) 640, 832
BseMII CTCAG 2 cut(s) 546, 732
BseNI ACTGG 3 cut(s) 517, 572, 1108
BseRI GAGGAG 2 cut(s) 97, 185
BseXI GCAGC 5 cut(s) 571, 712, 715, 871, 1122
Bsh1236I CGCG 1 cut(s) 204
Bsh1285I CGRYCG 1 cut(s) 45
BshFI GGCC 2 cut(s) 50, 140
BsiEI CGRYCG 1 cut(s) 45
BsiHKAI GWGCWC 2 cut(s) 14, 20
BsiHKCI CYCGRG 1 cut(s) 13
BsiSI CCGG 4 cut(s) 30, 41, 78, 451
BslI CCNNNNNNNGG 2 cut(s) 850, 1087
BsmAI GTCTC 4 cut(s) 170, 223, 580, 876
BsmBI CGTCTC 1 cut(s) 223
BsnI GGCC 2 cut(s) 50, 140
Bso31I GGTCTC 1 cut(s) 170
BsoBI CYCGRG 1 cut(s) 13
Bsp119I TTCGAA 1 cut(s) 959
Bsp1286I GDGCHC 2 cut(s) 14, 20
Bsp143I GATC 2 cut(s) 376, 421
BspACI CCGC 5 cut(s) 62, 202, 257, 349, 898
BspANI GGCC 2 cut(s) 50, 140
BspCNI CTCAG 2 cut(s) 547, 733
BspFNI CGCG 1 cut(s) 204
BspLI GGNNCC 3 cut(s) 34, 322, 449
BspPI GGATC 1 cut(s) 384
BspT104I TTCGAA 1 cut(s) 959
BspTNI GGTCTC 1 cut(s) 170
BsrDI GCAATG 2 cut(s) 640, 832
BsrFI RCCGGY 1 cut(s) 40
BsrI ACTGG 3 cut(s) 517, 572, 1108
BssAI RCCGGY 1 cut(s) 40
BssMI GATC 2 cut(s) 376, 421
BssNI GRCGYC 1 cut(s) 213
Bst4CI ACNGT 5 cut(s) 407, 496, 722, 950, 1022
Bst6I CTCTTC 3 cut(s) 115, 157, 168
BstACI GRCGYC 1 cut(s) 213
BstBAI YACGTR 1 cut(s) 26
BstBI TTCGAA 1 cut(s) 959
BstC8I GCNNGC 4 cut(s) 83, 138, 804, 1062
BstDEI CTNAG 2 cut(s) 555, 741
BstF5I GGATG 3 cut(s) 113, 536, 759
BstFNI CGCG 1 cut(s) 204
BstKTI GATC 2 cut(s) 379, 424
BstMAI GTCTC 4 cut(s) 170, 223, 580, 876
BstMBI GATC 2 cut(s) 376, 421
BstMCI CGRYCG 1 cut(s) 45
BstMWI GCNNNNNNNGC 1 cut(s) 87
BstNSI RCATGY 2 cut(s) 530, 806
BstSCI CCNGG 2 cut(s) 76, 449
BstSFI CTRYAG 2 cut(s) 307, 544
BstUI CGCG 1 cut(s) 204
BstV1I GCAGC 5 cut(s) 571, 712, 715, 871, 1122
BsuRI GGCC 2 cut(s) 50, 140
BtsCI GGATG 3 cut(s) 113, 536, 759
BtsI GCAGTG 1 cut(s) 1095
BtsIMutI CAGTG 4 cut(s) 510, 946, 1009, 1095
Cac8I GCNNGC 4 cut(s) 83, 138, 804, 1062
Cfr10I RCCGGY 1 cut(s) 40
Cfr13I GGNCC 3 cut(s) 360, 447, 663
Csp6I GTAC 2 cut(s) 269, 1048
CviAII CATG 5 cut(s) 190, 289, 527, 567, 803
CviQI GTAC 2 cut(s) 269, 1048
DdeI CTNAG 2 cut(s) 555, 741
DpnI GATC 2 cut(s) 378, 423
DpnII GATC 2 cut(s) 376, 421
DraI TTTAAA 1 cut(s) 1116
DrdI GACNNNNNNGTC 1 cut(s) 212
DseDI GACNNNNNNGTC 1 cut(s) 212
Eam1104I CTCTTC 3 cut(s) 115, 157, 168
EarI CTCTTC 3 cut(s) 115, 157, 168
Ecl136II GAGCTC 1 cut(s) 12
Eco147I AGGCCT 2 cut(s) 50, 140
Eco24I GRGCYC 1 cut(s) 14
Eco31I GGTCTC 1 cut(s) 170
Eco47I GGWCC 3 cut(s) 360, 447, 663
Eco53kI GAGCTC 1 cut(s) 12
Eco57I CTGAAG 1 cut(s) 37
Eco72I CACGTG 1 cut(s) 26
Eco88I CYCGRG 1 cut(s) 13
EcoICRI GAGCTC 1 cut(s) 12
EcoRI GAATTC 3 cut(s) 609, 940, 955
EcoT38I GRGCYC 1 cut(s) 14
Esp3I CGTCTC 1 cut(s) 223
FaeI CATG 5 cut(s) 193, 292, 530, 570, 806
FatI CATG 5 cut(s) 189, 288, 526, 566, 802
FauNDI CATATG 1 cut(s) 295
Fnu4HI GCNGC 7 cut(s) 62, 257, 560, 701, 704, 860, 1136
FokI GGATG 3 cut(s) 100, 523, 766
FriOI GRGCYC 1 cut(s) 14
Fsp4HI GCNGC 7 cut(s) 62, 257, 560, 701, 704, 860, 1136
FspBI CTAG 4 cut(s) 87, 165, 464, 590
GluI GCNGC 7 cut(s) 62, 257, 560, 701, 704, 860, 1136
HaeIII GGCC 2 cut(s) 50, 140
HapII CCGG 4 cut(s) 30, 41, 78, 451
Hin1I GRCGYC 1 cut(s) 213
Hin1II CATG 5 cut(s) 193, 292, 530, 570, 806
HinfI GANTC 7 cut(s) 283, 312, 413, 668, 745, 992, 999
HpaII CCGG 4 cut(s) 30, 41, 78, 451
HphI GGTGA 1 cut(s) 887
Hpy166II GTNNAC 1 cut(s) 895
Hpy188I TCNGA 4 cut(s) 447, 615, 742, 954
Hpy188III TCNNGA 6 cut(s) 156, 358, 410, 581, 751, 996
Hpy8I GTNNAC 1 cut(s) 895
Hpy99I CGWCG 2 cut(s) 209, 218
HpyAV CCTTC 5 cut(s) 61, 75, 157, 334, 791
HpyCH4III ACNGT 5 cut(s) 407, 496, 722, 950, 1022
HpyCH4IV ACGT 2 cut(s) 25, 213
HpyCH4V TGCA 6 cut(s) 530, 627, 651, 878, 1100, 1138
HpyF10VI GCNNNNNNNGC 1 cut(s) 87
HpyF3I CTNAG 2 cut(s) 555, 741
HpySE526I ACGT 2 cut(s) 25, 213
Hsp92I GRCGYC 1 cut(s) 213
Hsp92II CATG 5 cut(s) 193, 292, 530, 570, 806
Kzo9I GATC 2 cut(s) 376, 421
LmnI GCTCC 2 cut(s) 32, 198
Lsp1109I GCAGC 5 cut(s) 571, 712, 715, 871, 1122
LweI GCATC 3 cut(s) 195, 336, 545
MaeI CTAG 4 cut(s) 87, 165, 464, 590
MaeII ACGT 2 cut(s) 25, 213
MalI GATC 2 cut(s) 378, 423
MboI GATC 2 cut(s) 376, 421
MboII GAAGA 5 cut(s) 102, 144, 185, 230, 663
MhlI GDGCHC 2 cut(s) 14, 20
MluCI AATT 9 cut(s) 168, 609, 886, 903, 926, 935, 940, 955, 1143
MlyI GAGTC 1 cut(s) 407
MmeI TCCRAC 2 cut(s) 741, 932
MroXI GAANNNNTTC 1 cut(s) 965
MseI TTAA 3 cut(s) 1041, 1115, 1142
MspI CCGG 4 cut(s) 30, 41, 78, 451
MspR9I CCNGG 2 cut(s) 78, 451
MvnI CGCG 1 cut(s) 204
MwoI GCNNNNNNNGC 1 cut(s) 87
NciI CCSGG 2 cut(s) 78, 451
NdeI CATATG 1 cut(s) 295
NdeII GATC 2 cut(s) 376, 421
NlaIII CATG 5 cut(s) 193, 292, 530, 570, 806
NlaIV GGNNCC 3 cut(s) 34, 322, 449
NmeAIII GCCGAG 1 cut(s) 33
NspI RCATGY 2 cut(s) 530, 806
NspV TTCGAA 1 cut(s) 959
PaeI GCATGC 1 cut(s) 806
PaeR7I CTCGAG 1 cut(s) 13
PceI AGGCCT 2 cut(s) 50, 140
PcsI WCGNNNNNNNCGW 3 cut(s) 11, 213, 227
PdmI GAANNNNTTC 1 cut(s) 965
PfeI GAWTC 6 cut(s) 283, 312, 668, 745, 992, 999
PflFI GACNNNGTC 1 cut(s) 215
PflMI CCANNNNNTGG 1 cut(s) 1087
PkrI GCNGC 7 cut(s) 63, 258, 561, 702, 705, 861, 1137
PleI GAGTC 1 cut(s) 407
PmaCI CACGTG 1 cut(s) 26
PmlI CACGTG 1 cut(s) 26
PpsI GAGTC 1 cut(s) 407
Ppu21I YACGTR 1 cut(s) 26
Psp124BI GAGCTC 1 cut(s) 14
PspCI CACGTG 1 cut(s) 26
PspN4I GGNNCC 3 cut(s) 34, 322, 449
PspPI GGNCC 3 cut(s) 360, 447, 663
PspXI VCTCGAGB 1 cut(s) 13
PsyI GACNNNGTC 1 cut(s) 215
RsaI GTAC 2 cut(s) 270, 1049
RsaNI GTAC 2 cut(s) 269, 1048
SacI GAGCTC 1 cut(s) 14
SaqAI TTAA 3 cut(s) 1041, 1115, 1142
SatI GCNGC 7 cut(s) 62, 257, 560, 701, 704, 860, 1136
Sau3AI GATC 2 cut(s) 376, 421
Sau96I GGNCC 3 cut(s) 360, 447, 663
SchI GAGTC 1 cut(s) 407
ScrFI CCNGG 2 cut(s) 78, 451
SduI GDGCHC 2 cut(s) 14, 20
SfaNI GCATC 3 cut(s) 195, 336, 545
SfcI CTRYAG 2 cut(s) 307, 544
Sfr274I CTCGAG 1 cut(s) 13
SfuI TTCGAA 1 cut(s) 959
SinI GGWCC 3 cut(s) 360, 447, 663
SlaI CTCGAG 1 cut(s) 13
SmlI CTYRAG 3 cut(s) 13, 581, 1073
SmoI CTYRAG 3 cut(s) 13, 581, 1073
SphI GCATGC 1 cut(s) 806
Sse9I AATT 9 cut(s) 168, 609, 886, 903, 926, 935, 940, 955, 1143
SseBI AGGCCT 2 cut(s) 50, 140
SsiI CCGC 5 cut(s) 62, 202, 257, 349, 898
SspMI CTAG 4 cut(s) 87, 165, 464, 590
SstI GAGCTC 1 cut(s) 14
StuI AGGCCT 2 cut(s) 50, 140
StyD4I CCNGG 2 cut(s) 76, 449
TaaI ACNGT 5 cut(s) 407, 496, 722, 950, 1022
TaiI ACGT 2 cut(s) 28, 216
TaqI TCGA 4 cut(s) 14, 45, 688, 959
TaqII GACCGA 2 cut(s) 377, 680
TasI AATT 9 cut(s) 168, 609, 886, 903, 926, 935, 940, 955, 1143
TatI WGTACW 1 cut(s) 1047
TauI GCSGC 2 cut(s) 64, 259
TfiI GAWTC 6 cut(s) 283, 312, 668, 745, 992, 999
Tru1I TTAA 3 cut(s) 1041, 1115, 1142
Tru9I TTAA 3 cut(s) 1041, 1115, 1142
TscAI CASTG 4 cut(s) 517, 953, 1009, 1102
TseI GCWGC 5 cut(s) 559, 700, 703, 859, 1135
TspDTI ATGAA 3 cut(s) 102, 316, 715
TspGWI ACGGA 1 cut(s) 219
TspRI CASTG 4 cut(s) 517, 953, 1009, 1102
Tth111I GACNNNGTC 1 cut(s) 215
Van91I CCANNNNNTGG 1 cut(s) 1087
VpaK11BI GGWCC 3 cut(s) 360, 447, 663
XapI RAATTY 3 cut(s) 609, 940, 955
XceI RCATGY 2 cut(s) 530, 806
XcmI CCANNNNNNNNNTGG 1 cut(s) 602
XhoI CTCGAG 1 cut(s) 13
XmnI GAANNNNTTC 1 cut(s) 965
XspI CTAG 4 cut(s) 87, 165, 464, 590
ZraI GACGTC 1 cut(s) 214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.