Prupe.3G094400_v2.0.a1

Brca1-associated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0013575
Physical Location & Seq
Forward (+)
7075444 .. 7081293
5850 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G094400.1

Sequence Viewer

Length: 1464 bp
ATGTCACCAAGTTCGAGCACGGCACATGCCGGAGCCTCGCCAGGCGAGGTTTTCAGGTCGCAGCCGGCGATTATCTCCGGCGATCTGAGTGACTCCTCATATTCGTCGTCTTCATCGATCTCCAACATCACGCAGGCCTTTCCTTTTTCTTCTGGAAACCCTAGAATCGAAGAGACCAGAGGCATCATGCTTCTCTACCGCGATGACGCCGACGTCGTTTCCACCTCGTCCTCTCACCTCCCCGTTGGAAGGAAGCCACTTGTTTGTGTGCTTGGAGTGCCGAATCACATGACATATGCAGACTTTTGCCAGTTTTGTGGTTCTTTCATTCAGCACATTTTGGAGATGCGGATTGTCAGGAATGATGGAATGGAAGATCGTTACAGCATTTTGATTAGGTTTGATAGTCAGGACTCCACAGATGATTTTTACAAGCATTTCAATGGAAGCCGATTTTCGTCGCTTGAGGTGGAGGCTTGTCGCATTCTCTTCACGGTAGATGTTCAATACACTGGTTCAATAGAACATGCACAGGCATCATCGGGAAGCTCAACTGAGCAGCCATCATGTCCAGTCTGTCTTGAGAGACTAGACCAAGATATGGGTGGAATTCTCACGACTATCTGCAATCATTCTTTCCATTGTTCTTGCATTTCAAAATGGACGGATTCTTCTTGTCCTGTATGTCGATATTGTCAGCAGCAGCCTGAAAAGTCTAACTGTTTTGTTTGCCAAACTTCTGAGAATTTGTGGATGTGTGTTATCTGTGGCTTTGTTGGCTGTGGGAGGTATAAAGAAGGACATGCCATAAGACATTGGAAAGATACAGAACATTGTTATTCTCTTGAATTGGAAACGCAGCGTGTGTGGGATTATGCTGGAGACAATTATGTTCACCGCCTAATTCAATCTAAAACTGATGGGAAATTGGTTGAATTGAATTCACCGTATAATGAGTGTGGAAGCTGCGAATGTGTAGATTCTGGATTCAGTGAGGCCCTTTTGAACAGTAAAGTAGAAGCGATTGTTAATGAGTACAATGAACTGCTTGCTACTCAACTTGAGAACCAAAAAGTGTATTTTGAATTGTTACTGCAAGAAGTTAAAGAAGGAACTGAAAGAGAAATTTCTAATGCTGTTGAGAAGGCTGTACCACTAAAGAAAATGCAGGCCAAGCTGGACAGATGTGTAAAAGAGAAGAAATTTCTTGATGATCTTAATGAAAACCTTTTAAAGAATCAGGAGATTTGGAAAGCAAAGATACTTGAAGCTGAAGAGAGGGAGAAAAAGACTTTGAAACTAAAGGATGATAAAATTAGCGACTTAGAAGAACAGCTTAGGAATTTGATGCTCTATCTTGAGACTGGGAAGACAATGGAGCAGCCATCGATATCAAATGAAATAAAGGATGGCACTGCCTTGCCAGCATCGATAGAGTTGTCGCAGCCATCAAGTAGCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
BRAP2 zf-RING_2 zf-UBP

Protein Analysis

488

Amino Acids

54.75

Weight (kDa)

5.09

Isoelectric Point (pI)

48.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013575)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26000 AT2G26000 AT2G26000
fragaria_vesca FvH4_6g29040
malus_domestica MD17G1218900.v1.1
prunus_persica Prupe.3G094400_v2.0.a1 Prupe.3G094400_v2.0.a1
pyrus_communis pycom17g22370
rosa_chinensis RchiOBHm_Chr2g0133711
rosa_laevigata RLG00000019358
rosa_multiflora Rmu_sc0013154.1_g000008
rosa_roxburghii Rroxscaffold_2G00110880
rosa_rugosa Rorug02G0313200
rosa_samantha Rh2AG366600 Rh2BG372300 Rh2CG349900 Rh2DG389200
rosa_wichuraiana Rw2G029830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 212
AatII GACGTC 1 cut(s) 216
AccB7I CCANNNNNTGG 1 cut(s) 601
AccII CGCG 1 cut(s) 201
AciI CCGC 3 cut(s) 199, 349, 898
AcsI RAATTY 6 cut(s) 609, 745, 940, 1125, 1202, 1342
AcuI CTGAAG 1 cut(s) 1293
AcyI GRCGYC 2 cut(s) 207, 213
AfaI GTAC 2 cut(s) 1037, 1152
AfiI CCNNNNNNNGG 2 cut(s) 249, 601
AjnI CCWGG 1 cut(s) 40
AluBI AGCT 5 cut(s) 549, 966, 1177, 1271, 1336
AluI AGCT 5 cut(s) 549, 966, 1177, 1271, 1336
Alw21I GWGCWC 1 cut(s) 20
Alw26I GTCTC 4 cut(s) 167, 580, 876, 1355
AoxI GGCC 3 cut(s) 135, 996, 1170
ApeKI GCWGC 8 cut(s) 61, 559, 700, 703, 859, 966, 1381, 1444
ApoI RAATTY 6 cut(s) 609, 745, 940, 1125, 1202, 1342
AspS9I GGNCC 1 cut(s) 997
AsuHPI GGTGA 3 cut(s) 227, 887, 936
BbsI GAAGAC 2 cut(s) 102, 1376
Bbv12I GWGCWC 1 cut(s) 20
BbvI GCAGC 8 cut(s) 73, 571, 712, 715, 871, 953, 1393, 1456
BccI CCATC 6 cut(s) 359, 571, 914, 1393, 1403, 1456
BceAI ACGGC 1 cut(s) 36
BciT130I CCWGG 1 cut(s) 42
BcoDI GTCTC 4 cut(s) 167, 580, 876, 1355
BfaI CTAG 2 cut(s) 162, 590
BisI GCNGC 8 cut(s) 62, 560, 701, 704, 860, 967, 1382, 1445
BlsI GCNGC 8 cut(s) 63, 561, 702, 705, 861, 968, 1383, 1446
Bme1390I CCNGG 1 cut(s) 42
BmgT120I GGNCC 1 cut(s) 997
BmiI GGNNCC 1 cut(s) 34
BmrFI CCNGG 1 cut(s) 42
BmrI ACTGGG 1 cut(s) 1374
BmsI GCATC 5 cut(s) 192, 336, 545, 1338, 1436
BmuI ACTGGG 1 cut(s) 1374
BpiI GAAGAC 2 cut(s) 102, 1376
BpmI CTGGAG 1 cut(s) 900
Bpu10I CCTNAGC 1 cut(s) 1337
BpuEI CTTGAG 4 cut(s) 485, 602, 1082, 1379
Bsa29I ATCGAT 3 cut(s) 116, 1388, 1430
BsaHI GRCGYC 2 cut(s) 207, 213
BsaI GGTCTC 1 cut(s) 167
Bsc4I CCNNNNNNNGG 2 cut(s) 249, 601
Bse118I RCCGGY 1 cut(s) 64
Bse1I ACTGG 4 cut(s) 310, 517, 572, 1369
BseBI CCWGG 1 cut(s) 42
BseCI ATCGAT 3 cut(s) 116, 1388, 1430
BseGI GGATG 3 cut(s) 759, 1312, 1414
BseLI CCNNNNNNNGG 2 cut(s) 249, 601
BseMII CTCAG 3 cut(s) 77, 546, 732
BseNI ACTGG 4 cut(s) 310, 517, 572, 1369
BseRI GAGGAG 1 cut(s) 85
BseXI GCAGC 8 cut(s) 73, 571, 712, 715, 871, 953, 1393, 1456
Bsh1236I CGCG 1 cut(s) 201
BshFI GGCC 3 cut(s) 137, 998, 1172
BshVI ATCGAT 3 cut(s) 116, 1388, 1430
BsiHKAI GWGCWC 1 cut(s) 20
BsiSI CCGG 3 cut(s) 30, 65, 78
BslI CCNNNNNNNGG 2 cut(s) 249, 601
BsmAI GTCTC 4 cut(s) 167, 580, 876, 1355
BsmI GAATGC 1 cut(s) 483
BsnI GGCC 3 cut(s) 137, 998, 1172
Bso31I GGTCTC 1 cut(s) 167
Bsp1286I GDGCHC 1 cut(s) 20
Bsp143I GATC 4 cut(s) 82, 117, 376, 1213
BspACI CCGC 3 cut(s) 199, 349, 898
BspANI GGCC 3 cut(s) 137, 998, 1172
BspCNI CTCAG 3 cut(s) 78, 547, 733
BspDI ATCGAT 3 cut(s) 116, 1388, 1430
BspFNI CGCG 1 cut(s) 201
BspLI GGNNCC 1 cut(s) 34
BspTNI GGTCTC 1 cut(s) 167
BsrFI RCCGGY 1 cut(s) 64
BsrI ACTGG 4 cut(s) 310, 517, 572, 1369
BssAI RCCGGY 1 cut(s) 64
BssMI GATC 4 cut(s) 82, 117, 376, 1213
BssNI GRCGYC 2 cut(s) 207, 213
Bst2UI CCWGG 1 cut(s) 42
Bst4CI ACNGT 4 cut(s) 496, 722, 948, 1010
Bst6I CTCTTC 3 cut(s) 165, 494, 1269
BstACI GRCGYC 2 cut(s) 207, 213
BstC8I GCNNGC 5 cut(s) 66, 135, 1050, 1170, 1425
BstDEI CTNAG 5 cut(s) 86, 555, 741, 1324, 1337
BstF5I GGATG 3 cut(s) 759, 1312, 1414
BstFNI CGCG 1 cut(s) 201
BstKTI GATC 4 cut(s) 85, 120, 379, 1216
BstMAI GTCTC 4 cut(s) 167, 580, 876, 1355
BstMBI GATC 4 cut(s) 82, 117, 376, 1213
BstMWI GCNNNNNNNGC 4 cut(s) 277, 777, 1174, 1424
BstNI CCWGG 1 cut(s) 42
BstNSI RCATGY 3 cut(s) 29, 530, 806
BstSCI CCNGG 1 cut(s) 40
BstUI CGCG 1 cut(s) 201
BstV1I GCAGC 8 cut(s) 73, 571, 712, 715, 871, 953, 1393, 1456
BstV2I GAAGAC 2 cut(s) 102, 1376
BstXI CCANNNNNNTGG 1 cut(s) 317
Bsu15I ATCGAT 3 cut(s) 116, 1388, 1430
BsuRI GGCC 3 cut(s) 137, 998, 1172
BsuTUI ATCGAT 3 cut(s) 116, 1388, 1430
BtgZI GCGATG 1 cut(s) 216
BtsCI GGATG 3 cut(s) 759, 1312, 1414
BtsI GCAGTG 1 cut(s) 1413
BtsIMutI CAGTG 3 cut(s) 510, 997, 1413
Cac8I GCNNGC 5 cut(s) 66, 135, 1050, 1170, 1425
Cfr10I RCCGGY 1 cut(s) 64
Cfr13I GGNCC 1 cut(s) 997
ClaI ATCGAT 3 cut(s) 116, 1388, 1430
CseI GACGC 1 cut(s) 215
Csp6I GTAC 2 cut(s) 1036, 1151
CviAII CATG 6 cut(s) 26, 187, 289, 527, 567, 803
CviQI GTAC 2 cut(s) 1036, 1151
DdeI CTNAG 5 cut(s) 86, 555, 741, 1324, 1337
DpnI GATC 4 cut(s) 84, 119, 378, 1215
DpnII GATC 4 cut(s) 82, 117, 376, 1213
DraI TTTAAA 1 cut(s) 1233
DrdI GACNNNNNNGTC 1 cut(s) 212
DseDI GACNNNNNNGTC 1 cut(s) 212
Eam1104I CTCTTC 3 cut(s) 165, 494, 1269
EarI CTCTTC 3 cut(s) 165, 494, 1269
Eco147I AGGCCT 1 cut(s) 137
Eco31I GGTCTC 1 cut(s) 167
Eco32I GATATC 1 cut(s) 1392
Eco57I CTGAAG 1 cut(s) 1293
EcoO109I RGGNCCY 1 cut(s) 997
EcoRI GAATTC 2 cut(s) 609, 940
EcoRII CCWGG 1 cut(s) 40
EcoRV GATATC 1 cut(s) 1392
FaeI CATG 6 cut(s) 29, 190, 292, 530, 570, 806
FalI AAGNNNNNCTT 2 cut(s) 1320, 1352
FatI CATG 6 cut(s) 25, 186, 288, 526, 566, 802
FauNDI CATATG 1 cut(s) 295
Fnu4HI GCNGC 8 cut(s) 62, 560, 701, 704, 860, 967, 1382, 1445
FokI GGATG 3 cut(s) 766, 1319, 1421
Fsp4HI GCNGC 8 cut(s) 62, 560, 701, 704, 860, 967, 1382, 1445
FspBI CTAG 2 cut(s) 162, 590
GluI GCNGC 8 cut(s) 62, 560, 701, 704, 860, 967, 1382, 1445
GsuI CTGGAG 1 cut(s) 900
HaeIII GGCC 3 cut(s) 137, 998, 1172
HapII CCGG 3 cut(s) 30, 65, 78
HgaI GACGC 1 cut(s) 215
Hin1I GRCGYC 2 cut(s) 207, 213
Hin1II CATG 6 cut(s) 29, 190, 292, 530, 570, 806
HinfI GANTC 8 cut(s) 92, 165, 283, 413, 668, 980, 987, 1237
HpaII CCGG 3 cut(s) 30, 65, 78
HphI GGTGA 3 cut(s) 227, 887, 936
Hpy166II GTNNAC 1 cut(s) 895
Hpy188I TCNGA 2 cut(s) 87, 742
Hpy8I GTNNAC 1 cut(s) 895
Hpy99I CGWCG 4 cut(s) 109, 215, 218, 463
HpyAV CCTTC 4 cut(s) 243, 791, 1103, 1138
HpyCH4III ACNGT 4 cut(s) 496, 722, 948, 1010
HpyCH4IV ACGT 1 cut(s) 213
HpyCH4V TGCA 6 cut(s) 299, 530, 627, 651, 1096, 1168
HpyF10VI GCNNNNNNNGC 4 cut(s) 277, 777, 1174, 1424
HpyF3I CTNAG 5 cut(s) 86, 555, 741, 1324, 1337
HpySE526I ACGT 1 cut(s) 213
Hsp92I GRCGYC 2 cut(s) 207, 213
Hsp92II CATG 6 cut(s) 29, 190, 292, 530, 570, 806
KroI GCCGGC 1 cut(s) 64
KroNI GCCGGC 1 cut(s) 66
Kzo9I GATC 4 cut(s) 82, 117, 376, 1213
LmnI GCTCC 2 cut(s) 32, 1378
Lsp1109I GCAGC 8 cut(s) 73, 571, 712, 715, 871, 953, 1393, 1456
LweI GCATC 5 cut(s) 192, 336, 545, 1338, 1436
MaeI CTAG 2 cut(s) 162, 590
MaeII ACGT 1 cut(s) 213
MaeIII GTNAC 4 cut(s) 3, 89, 380, 1089
MalI GATC 4 cut(s) 84, 119, 378, 1215
MboI GATC 4 cut(s) 82, 117, 376, 1213
MhlI GDGCHC 1 cut(s) 20
MlyI GAGTC 2 cut(s) 86, 407
MmeI TCCRAC 2 cut(s) 147, 226
MroNI GCCGGC 1 cut(s) 64
MseI TTAA 4 cut(s) 1029, 1104, 1218, 1232
MslI CAYNNNNRTG 1 cut(s) 441
MspI CCGG 3 cut(s) 30, 65, 78
MspR9I CCNGG 1 cut(s) 42
Mva1269I GAATGC 1 cut(s) 483
MvaI CCWGG 1 cut(s) 42
MvnI CGCG 1 cut(s) 201
MwoI GCNNNNNNNGC 4 cut(s) 277, 777, 1174, 1424
NaeI GCCGGC 1 cut(s) 66
NdeI CATATG 1 cut(s) 295
NdeII GATC 4 cut(s) 82, 117, 376, 1213
NgoMIV GCCGGC 1 cut(s) 64
NlaIII CATG 6 cut(s) 29, 190, 292, 530, 570, 806
NlaIV GGNNCC 1 cut(s) 34
NmuCI GTSAC 2 cut(s) 3, 89
NspI RCATGY 3 cut(s) 29, 530, 806
PceI AGGCCT 1 cut(s) 137
PcsI WCGNNNNNNNCGW 3 cut(s) 65, 113, 213
PctI GAATGC 1 cut(s) 483
PdiI GCCGGC 1 cut(s) 66
PfeI GAWTC 6 cut(s) 165, 283, 668, 980, 987, 1237
PflMI CCANNNNNTGG 1 cut(s) 601
PkrI GCNGC 8 cut(s) 63, 561, 702, 705, 861, 968, 1383, 1446
PleI GAGTC 2 cut(s) 86, 407
PpsI GAGTC 2 cut(s) 86, 407
Psp6I CCWGG 1 cut(s) 40
PspGI CCWGG 1 cut(s) 40
PspN4I GGNNCC 1 cut(s) 34
PspPI GGNCC 1 cut(s) 997
RsaI GTAC 2 cut(s) 1037, 1152
RsaNI GTAC 2 cut(s) 1036, 1151
RseI CAYNNNNRTG 1 cut(s) 441
SaqAI TTAA 4 cut(s) 1029, 1104, 1218, 1232
SatI GCNGC 8 cut(s) 62, 560, 701, 704, 860, 967, 1382, 1445
Sau3AI GATC 4 cut(s) 82, 117, 376, 1213
Sau96I GGNCC 1 cut(s) 997
SchI GAGTC 2 cut(s) 86, 407
ScrFI CCNGG 1 cut(s) 42
SduI GDGCHC 1 cut(s) 20
SfaNI GCATC 5 cut(s) 192, 336, 545, 1338, 1436
SmiMI CAYNNNNRTG 1 cut(s) 441
SmlI CTYRAG 4 cut(s) 464, 581, 1061, 1358
SmoI CTYRAG 4 cut(s) 464, 581, 1061, 1358
SseBI AGGCCT 1 cut(s) 137
SsiI CCGC 3 cut(s) 199, 349, 898
SspMI CTAG 2 cut(s) 162, 590
StuI AGGCCT 1 cut(s) 137
StyD4I CCNGG 1 cut(s) 40
TaaI ACNGT 4 cut(s) 496, 722, 948, 1010
TaiI ACGT 1 cut(s) 216
TaqI TCGA 6 cut(s) 14, 116, 168, 688, 1388, 1430
TatI WGTACW 1 cut(s) 1035
TfiI GAWTC 6 cut(s) 165, 283, 668, 980, 987, 1237
Tru1I TTAA 4 cut(s) 1029, 1104, 1218, 1232
Tru9I TTAA 4 cut(s) 1029, 1104, 1218, 1232
TscAI CASTG 3 cut(s) 517, 997, 1420
TseFI GTSAC 2 cut(s) 3, 89
TseI GCWGC 8 cut(s) 61, 559, 700, 703, 859, 966, 1381, 1444
Tsp45I GTSAC 2 cut(s) 3, 89
TspDTI ATGAA 5 cut(s) 102, 316, 1056, 1236, 1413
TspGWI ACGGA 1 cut(s) 680
TspRI CASTG 3 cut(s) 517, 997, 1420
Van91I CCANNNNNTGG 1 cut(s) 601
XapI RAATTY 6 cut(s) 609, 745, 940, 1125, 1202, 1342
XceI RCATGY 3 cut(s) 29, 530, 806
XcmI CCANNNNNNNNNTGG 1 cut(s) 602
XspI CTAG 2 cut(s) 162, 590
ZraI GACGTC 1 cut(s) 214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.