MD17G1218900.v1.1

Brca1-associated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0013575
Physical Location & Seq
Forward (+)
26622916 .. 26628617
5702 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1218900.v1.1.491

Sequence Viewer

Length: 1554 bp
ATGTCACCGAGTTCGAGCACAGCTCATTCCGGAGCCTCCCCCGGCGAGGTTTTCCGGTCGCCGCCTCCTATCTCCAGCGATTTCAGCGACTCCTCATATTCCCCCATCTCCAACATCACGCAGGCCTTTCCTTTCTCTTCTGGAAACCCTAGAATCGAAGAGACCAGAGGCATCATGCTTCTCTACCGCGACGACGCCGATGCCGACGTCATCCCCACCTCCCCGTCTAATCTCCCCGTTGGAAGGAAGCCCCTTGTTTGTGTGCTTGGAGTTCCGAATCACATGACATATGCGGACTTCTGCCAGTTTTGTGGTTCATTTATTCAGCATATTTTGGAGATGCGGATTGTCAGGAATGACGGGATGGAGGATCGTTACAGCATTTTGATAAGGTTTGATAGTCAGGACTCGACAGATAATTTTTACAAGCATTTCAACGGGAGACGATTCTCGTCGCTTGAGGTGGAGGTTTGTCGTATTCTTTTCACGGTAGATGTTCAATACACTGGTTCGATAGAACATGCGCAAGCATCATCAGGAAGCTCAACTGAGCAGCCATCATGTCCAGTTTGTCTTGAGAGACTGGACCAAGATATGGAAGGAATCCTCACGACTATTTGCAATCATTCATTTCATTGTTCCTGCATTTCAAAATGGACAGATTCTTCATGTCCTGTATGTCGATATTGTCAGCAACAGCCTGAAAAGTCCAACTGTTTTCTTTGTCAAACTTCTGAGAATCTCTGGATGTGTGTTATCTGTGGCTTTGTTGGTTGTGGGAGGTATAAAGAAGGACATGCAATAAGGCATTGGAAAGAAACAGAACATTGTTATTCTCTTGAATTGGAAACACAACGTGTGTGGGATTATGCTGGAGACAATTATGTTCACCGCCTAATTCAATCCAAAACTGATGGGAAATTGGTTGAATTGAATTCACCTTTTATTGGATGTCAAAGTTGTGACTGCGTAGATTCTGAATACAGTGAGGCACTTTTGAACAGTAAAGTAGAAGCTATTGTTAATGAATACAATGAACTGCTTGCTACTCAACTTGAGAACCAAAAAGTGTATTTTGAGTCGTTACTTCAAGAAGTTAAAGAAGGAACTGAACAAGAAATTTCTAATGCTGTGGAGAAGGCTGTTCCACTAAAGAAATTGCAGGCCAAGCTGGACAGGTGTGTAAAAGAGAAGAAATTTCTTGATGATCTTAATGAAAACCTTTTAAAAAACCAGGAGATTTGGAAAGCAAAGATACTTGAATCTGAAGAGAGGGAGAGAAAGACTTTGAAACTAAGGGACGATAAAATTAGCGACTTAGAAGAGCAGCTTGGGAATTTGATGATGTACCTTGAGGCTGGGAAGACGGTGGAGCAGCTATCAACATCAGATGAAATAAAGGATGGGACTGCCTTGCCAGTATCGGTGGAGCCACCACAGCCATCAGTACGGAATGAAATTGGTGATGCCACTGTCACTTCTCCGGAAGTTTCCAATGAATGTATAGAAGAGGGCTCAAAGACTAATAACAGGAGGAGGAGACGCCAAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
BRAP2 zf-RING_2 zf-UBP

Protein Analysis

518

Amino Acids

58.42

Weight (kDa)

4.99

Isoelectric Point (pI)

55.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BRAP2 PF07576 78 - 175 3.2e-40 BRCA1-associated protein 2
zf-RING_2 PF13639 187 - 228 1.9e-08 Ring finger domain
zf-rbx1 PF12678 187 - 228 1.5e-06 RING-H2 zinc finger domain
zf-UBP PF02148 239 - 298 4e-23 Zn-finger in ubiquitin-hydrolases and other protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013575)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26000 AT2G26000 AT2G26000
fragaria_vesca FvH4_6g29040
malus_domestica MD17G1218900.v1.1
prunus_persica Prupe.3G094400_v2.0.a1 Prupe.3G094400_v2.0.a1
pyrus_communis pycom17g22370
rosa_chinensis RchiOBHm_Chr2g0133711
rosa_laevigata RLG00000019358
rosa_multiflora Rmu_sc0013154.1_g000008
rosa_roxburghii Rroxscaffold_2G00110880
rosa_rugosa Rorug02G0313200
rosa_samantha Rh2AG366600 Rh2BG372300 Rh2CG349900 Rh2DG389200
rosa_wichuraiana Rw2G029830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 210
Acc16I TGCGCA 1 cut(s) 525
AccB7I CCANNNNNTGG 1 cut(s) 595
AccII CGCG 1 cut(s) 189
AccIII TCCGGA 2 cut(s) 29, 1483
AciI CCGC 5 cut(s) 62, 187, 293, 343, 892
AclWI GGATC 1 cut(s) 378
AcsI RAATTY 4 cut(s) 934, 1119, 1196, 1336
AcuI CTGAAG 1 cut(s) 1287
AcyI GRCGYC 3 cut(s) 195, 207, 1543
AdeI CACNNNGTG 1 cut(s) 857
AfaI GTAC 2 cut(s) 1349, 1449
AfiI CCNNNNNNNGG 4 cut(s) 46, 243, 595, 947
AflIII ACRYGT 1 cut(s) 856
AjnI CCWGG 1 cut(s) 1233
AjuI GAANNNNNNNTTGG 2 cut(s) 1314, 1346
AluBI AGCT 6 cut(s) 23, 543, 1016, 1171, 1330, 1378
AluI AGCT 6 cut(s) 23, 543, 1016, 1171, 1330, 1378
Alw21I GWGCWC 1 cut(s) 20
Alw26I GTCTC 5 cut(s) 155, 436, 574, 870, 1534
AlwI GGATC 1 cut(s) 378
Aor13HI TCCGGA 2 cut(s) 29, 1483
AoxI GGCC 2 cut(s) 123, 1164
ApeKI GCWGC 3 cut(s) 553, 1327, 1375
ApoI RAATTY 4 cut(s) 934, 1119, 1196, 1336
AspLEI GCGC 1 cut(s) 526
AspS9I GGNCC 1 cut(s) 586
AsuC2I CCSGG 1 cut(s) 42
AsuHPI GGTGA 3 cut(s) 881, 930, 1475
AvaII GGWCC 1 cut(s) 586
BanII GRGCYC 1 cut(s) 1517
BbsI GAAGAC 1 cut(s) 1370
Bbv12I GWGCWC 1 cut(s) 20
BbvI GCAGC 3 cut(s) 565, 1339, 1387
BccI CCATC 6 cut(s) 113, 358, 565, 908, 1397, 1450
BcgI CGANNNNNNTGC 2 cut(s) 182, 216
BciT130I CCWGG 1 cut(s) 1235
BcnI CCSGG 1 cut(s) 42
BcoDI GTCTC 5 cut(s) 155, 436, 574, 870, 1534
BfaI CTAG 1 cut(s) 150
BisI GCNGC 4 cut(s) 62, 554, 1328, 1376
BlsI GCNGC 4 cut(s) 63, 555, 1329, 1377
Bme1390I CCNGG 2 cut(s) 42, 1235
Bme18I GGWCC 1 cut(s) 586
BmgT120I GGNCC 1 cut(s) 586
BmiI GGNNCC 2 cut(s) 34, 1431
BmrFI CCNGG 2 cut(s) 42, 1235
BmsI GCATC 5 cut(s) 180, 190, 330, 539, 1456
BpiI GAAGAC 1 cut(s) 1370
BplI GAGNNNNNCTC 2 cut(s) 7, 39
BpmI CTGGAG 2 cut(s) 58, 894
BpuEI CTTGAG 4 cut(s) 479, 596, 1076, 1373
BpuMI CCSGG 1 cut(s) 42
BsaHI GRCGYC 3 cut(s) 195, 207, 1543
BsaI GGTCTC 1 cut(s) 155
BsaJI CCNNGG 2 cut(s) 40, 1546
BsaWI WCCGGW 3 cut(s) 29, 54, 1483
Bsc4I CCNNNNNNNGG 4 cut(s) 46, 243, 595, 947
Bse1I ACTGG 5 cut(s) 304, 511, 566, 588, 1418
BseAI TCCGGA 2 cut(s) 29, 1483
BseBI CCWGG 1 cut(s) 1235
BseDI CCNNGG 2 cut(s) 40, 1546
BseGI GGATG 5 cut(s) 210, 369, 753, 956, 1408
BseLI CCNNNNNNNGG 4 cut(s) 46, 243, 595, 947
BseMII CTCAG 2 cut(s) 540, 726
BseNI ACTGG 5 cut(s) 304, 511, 566, 588, 1418
BseRI GAGGAG 3 cut(s) 82, 1549, 1552
BseXI GCAGC 3 cut(s) 565, 1339, 1387
BseYI CCCAGC 1 cut(s) 1358
Bsh1236I CGCG 1 cut(s) 189
Bsh1285I CGRYCG 1 cut(s) 59
BshFI GGCC 2 cut(s) 125, 1166
BsiEI CGRYCG 1 cut(s) 59
BsiHKAI GWGCWC 1 cut(s) 20
BsiSI CCGG 4 cut(s) 30, 42, 55, 1484
BslFI GGGAC 2 cut(s) 1313, 1420
BslI CCNNNNNNNGG 4 cut(s) 46, 243, 595, 947
BsmAI GTCTC 5 cut(s) 155, 436, 574, 870, 1534
BsmBI CGTCTC 2 cut(s) 436, 1534
BsmFI GGGAC 2 cut(s) 1313, 1420
BsnI GGCC 2 cut(s) 125, 1166
Bso31I GGTCTC 1 cut(s) 155
Bsp1286I GDGCHC 2 cut(s) 20, 1517
Bsp13I TCCGGA 2 cut(s) 29, 1483
Bsp143I GATC 2 cut(s) 370, 1207
BspACI CCGC 5 cut(s) 62, 187, 293, 343, 892
BspANI GGCC 2 cut(s) 125, 1166
BspCNI CTCAG 2 cut(s) 541, 727
BspEI TCCGGA 2 cut(s) 29, 1483
BspFNI CGCG 1 cut(s) 189
BspLI GGNNCC 2 cut(s) 34, 1431
BspPI GGATC 1 cut(s) 378
BspQI GCTCTTC 1 cut(s) 1317
BspTNI GGTCTC 1 cut(s) 155
BsrI ACTGG 5 cut(s) 304, 511, 566, 588, 1418
BssECI CCNNGG 2 cut(s) 40, 1546
BssMI GATC 2 cut(s) 370, 1207
BssNI GRCGYC 3 cut(s) 195, 207, 1543
BssT1I CCWWGG 1 cut(s) 1546
Bst2UI CCWGG 1 cut(s) 1235
Bst4CI ACNGT 6 cut(s) 490, 716, 986, 1004, 1369, 1474
Bst6I CTCTTC 5 cut(s) 142, 153, 1263, 1317, 1503
BstACI GRCGYC 3 cut(s) 195, 207, 1543
BstC8I GCNNGC 4 cut(s) 123, 528, 1044, 1164
BstDEI CTNAG 4 cut(s) 549, 735, 1295, 1318
BstF5I GGATG 5 cut(s) 210, 369, 753, 956, 1408
BstFNI CGCG 1 cut(s) 189
BstHHI GCGC 1 cut(s) 526
BstKTI GATC 2 cut(s) 373, 1210
BstMAI GTCTC 5 cut(s) 155, 436, 574, 870, 1534
BstMBI GATC 2 cut(s) 370, 1207
BstMCI CGRYCG 1 cut(s) 59
BstMWI GCNNNNNNNGC 3 cut(s) 84, 1168, 1438
BstNI CCWGG 1 cut(s) 1235
BstNSI RCATGY 2 cut(s) 524, 800
BstSCI CCNGG 2 cut(s) 40, 1233
BstUI CGCG 1 cut(s) 189
BstV1I GCAGC 3 cut(s) 565, 1339, 1387
BstV2I GAAGAC 1 cut(s) 1370
BstXI CCANNNNNNTGG 1 cut(s) 311
BsuRI GGCC 2 cut(s) 125, 1166
BtsCI GGATG 5 cut(s) 210, 369, 753, 956, 1408
BtsIMutI CAGTG 3 cut(s) 504, 991, 1470
Cac8I GCNNGC 4 cut(s) 123, 528, 1044, 1164
CfoI GCGC 1 cut(s) 526
Cfr13I GGNCC 1 cut(s) 586
CseI GACGC 1 cut(s) 203
Csp6I GTAC 2 cut(s) 1348, 1448
CspCI CAANNNNNGTGG 2 cut(s) 842, 877
CviAII CATG 6 cut(s) 175, 283, 521, 561, 669, 797
CviQI GTAC 2 cut(s) 1348, 1448
DdeI CTNAG 4 cut(s) 549, 735, 1295, 1318
DpnI GATC 2 cut(s) 372, 1209
DpnII GATC 2 cut(s) 370, 1207
DraI TTTAAA 1 cut(s) 1227
DraIII CACNNNGTG 1 cut(s) 857
Eam1104I CTCTTC 5 cut(s) 142, 153, 1263, 1317, 1503
EarI CTCTTC 5 cut(s) 142, 153, 1263, 1317, 1503
Eco130I CCWWGG 1 cut(s) 1546
Eco147I AGGCCT 1 cut(s) 125
Eco24I GRGCYC 1 cut(s) 1517
Eco31I GGTCTC 1 cut(s) 155
Eco47I GGWCC 1 cut(s) 586
Eco57I CTGAAG 1 cut(s) 1287
EcoRI GAATTC 1 cut(s) 934
EcoRII CCWGG 1 cut(s) 1233
EcoT14I CCWWGG 1 cut(s) 1546
EcoT38I GRGCYC 1 cut(s) 1517
ErhI CCWWGG 1 cut(s) 1546
Esp3I CGTCTC 2 cut(s) 436, 1534
FaeI CATG 6 cut(s) 178, 286, 524, 564, 672, 800
FalI AAGNNNNNCTT 2 cut(s) 1314, 1346
FaqI GGGAC 2 cut(s) 1313, 1420
FatI CATG 6 cut(s) 174, 282, 520, 560, 668, 796
FauNDI CATATG 1 cut(s) 289
Fnu4HI GCNGC 4 cut(s) 62, 554, 1328, 1376
FokI GGATG 5 cut(s) 197, 376, 760, 963, 1415
FriOI GRGCYC 1 cut(s) 1517
Fsp4HI GCNGC 4 cut(s) 62, 554, 1328, 1376
FspBI CTAG 1 cut(s) 150
FspI TGCGCA 1 cut(s) 525
GlaI GCGC 1 cut(s) 525
GluI GCNGC 4 cut(s) 62, 554, 1328, 1376
GsaI CCCAGC 1 cut(s) 1362
GsuI CTGGAG 2 cut(s) 58, 894
HaeIII GGCC 2 cut(s) 125, 1166
HapII CCGG 4 cut(s) 30, 42, 55, 1484
HgaI GACGC 1 cut(s) 203
HhaI GCGC 1 cut(s) 526
Hin1I GRCGYC 3 cut(s) 195, 207, 1543
Hin1II CATG 6 cut(s) 178, 286, 524, 564, 672, 800
Hin6I GCGC 1 cut(s) 524
HinP1I GCGC 1 cut(s) 524
HpaII CCGG 4 cut(s) 30, 42, 55, 1484
HphI GGTGA 3 cut(s) 881, 930, 1475
Hpy166II GTNNAC 1 cut(s) 889
Hpy188I TCNGA 5 cut(s) 276, 736, 979, 1267, 1390
Hpy8I GTNNAC 1 cut(s) 889
Hpy99I CGWCG 4 cut(s) 194, 197, 209, 457
HpyAV CCTTC 5 cut(s) 237, 593, 785, 1097, 1132
HpyCH4III ACNGT 6 cut(s) 490, 716, 986, 1004, 1369, 1474
HpyCH4IV ACGT 2 cut(s) 207, 856
HpyCH4V TGCA 4 cut(s) 621, 645, 800, 1162
HpyF10VI GCNNNNNNNGC 3 cut(s) 84, 1168, 1438
HpyF3I CTNAG 4 cut(s) 549, 735, 1295, 1318
HpySE526I ACGT 2 cut(s) 207, 856
Hsp92I GRCGYC 3 cut(s) 195, 207, 1543
Hsp92II CATG 6 cut(s) 178, 286, 524, 564, 672, 800
HspAI GCGC 1 cut(s) 524
Kpn2I TCCGGA 2 cut(s) 29, 1483
Kzo9I GATC 2 cut(s) 370, 1207
LguI GCTCTTC 1 cut(s) 1317
LmnI GCTCC 3 cut(s) 32, 1372, 1429
Lsp1109I GCAGC 3 cut(s) 565, 1339, 1387
LweI GCATC 5 cut(s) 180, 190, 330, 539, 1456
MaeI CTAG 1 cut(s) 150
MaeII ACGT 2 cut(s) 207, 856
MaeIII GTNAC 5 cut(s) 3, 374, 962, 1083, 1474
MalI GATC 2 cut(s) 372, 1209
MboI GATC 2 cut(s) 370, 1207
MboII GAAGA 8 cut(s) 129, 170, 657, 1204, 1280, 1334, 1375, 1520
MhlI GDGCHC 2 cut(s) 20, 1517
MlyI GAGTC 3 cut(s) 83, 401, 1088
MmeI TCCRAC 3 cut(s) 135, 220, 735
MroI TCCGGA 2 cut(s) 29, 1483
MseI TTAA 4 cut(s) 1023, 1098, 1212, 1226
MspI CCGG 4 cut(s) 30, 42, 55, 1484
MspR9I CCNGG 2 cut(s) 42, 1235
MvaI CCWGG 1 cut(s) 1235
MvnI CGCG 1 cut(s) 189
MwoI GCNNNNNNNGC 3 cut(s) 84, 1168, 1438
NciI CCSGG 1 cut(s) 42
NdeI CATATG 1 cut(s) 289
NdeII GATC 2 cut(s) 370, 1207
NlaIII CATG 6 cut(s) 178, 286, 524, 564, 672, 800
NlaIV GGNNCC 2 cut(s) 34, 1431
NmuCI GTSAC 3 cut(s) 3, 962, 1474
NsbI TGCGCA 1 cut(s) 525
NspI RCATGY 2 cut(s) 524, 800
PceI AGGCCT 1 cut(s) 125
PciSI GCTCTTC 1 cut(s) 1317
PcsI WCGNNNNNNNCGW 1 cut(s) 201
PfeI GAWTC 8 cut(s) 153, 277, 447, 603, 662, 739, 974, 1262
PflMI CCANNNNNTGG 1 cut(s) 595
PkrI GCNGC 4 cut(s) 63, 555, 1329, 1377
PleI GAGTC 3 cut(s) 83, 401, 1087
PpsI GAGTC 3 cut(s) 83, 401, 1087
Psp6I CCWGG 1 cut(s) 1233
PspFI CCCAGC 1 cut(s) 1358
PspGI CCWGG 1 cut(s) 1233
PspN4I GGNNCC 2 cut(s) 34, 1431
PspPI GGNCC 1 cut(s) 586
RsaI GTAC 2 cut(s) 1349, 1449
RsaNI GTAC 2 cut(s) 1348, 1448
SapI GCTCTTC 1 cut(s) 1317
SaqAI TTAA 4 cut(s) 1023, 1098, 1212, 1226
SatI GCNGC 4 cut(s) 62, 554, 1328, 1376
Sau3AI GATC 2 cut(s) 370, 1207
Sau96I GGNCC 1 cut(s) 586
SchI GAGTC 3 cut(s) 83, 401, 1088
ScrFI CCNGG 2 cut(s) 42, 1235
SduI GDGCHC 2 cut(s) 20, 1517
SfaNI GCATC 5 cut(s) 180, 190, 330, 539, 1456
SinI GGWCC 1 cut(s) 586
SmlI CTYRAG 4 cut(s) 458, 575, 1055, 1352
SmoI CTYRAG 4 cut(s) 458, 575, 1055, 1352
SseBI AGGCCT 1 cut(s) 125
SsiI CCGC 5 cut(s) 62, 187, 293, 343, 892
SspMI CTAG 1 cut(s) 150
StuI AGGCCT 1 cut(s) 125
StyD4I CCNGG 2 cut(s) 40, 1233
StyI CCWWGG 1 cut(s) 1546
TaaI ACNGT 6 cut(s) 490, 716, 986, 1004, 1369, 1474
TaiI ACGT 2 cut(s) 210, 859
TaqI TCGA 5 cut(s) 14, 156, 410, 512, 682
TauI GCSGC 1 cut(s) 64
TfiI GAWTC 8 cut(s) 153, 277, 447, 603, 662, 739, 974, 1262
Tru1I TTAA 4 cut(s) 1023, 1098, 1212, 1226
Tru9I TTAA 4 cut(s) 1023, 1098, 1212, 1226
TscAI CASTG 3 cut(s) 511, 991, 1477
TseFI GTSAC 3 cut(s) 3, 962, 1474
TseI GCWGC 3 cut(s) 553, 1327, 1375
Tsp45I GTSAC 3 cut(s) 3, 962, 1474
TspGWI ACGGA 1 cut(s) 1465
TspRI CASTG 3 cut(s) 511, 991, 1477
Van91I CCANNNNNTGG 1 cut(s) 595
VpaK11BI GGWCC 1 cut(s) 586
XapI RAATTY 4 cut(s) 934, 1119, 1196, 1336
XceI RCATGY 2 cut(s) 524, 800
XspI CTAG 1 cut(s) 150
ZraI GACGTC 1 cut(s) 208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.