pycom17g22370

Brca1-associated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0013575
Physical Location & Seq
Forward (+)
20772325 .. 20777418
5094 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g22370.8

Sequence Viewer

Length: 1548 bp
ATGTCACCGAGTTCGAGCACAGCTCATTCCGGAGCCTCCCCTGGCGAAGTTTTCCGGTCGCCGCCTTCTATCTCCAGCGATTTCAGCGAATCCTCATATTCCTCCATCTCCAACATCACGCAGGCCTTTCCTTTCTCTTCTGGAAACCCTAGAATCGAAGAGACCAGAGGCATTATGCTTCTCTACCGCGACGACGCCGATGCCGACGTCATCCCCACCTCCCCGTCTAATCTCCCCGTTGGAAGGAAGCCACTTGTTTGTGTGCTTGGAGTTCCGAATCACATGACATATGCGGACTTCTGCCAGTTTTGTGGTTCATTTATTCAGCATATTTTGGAGATGCGGATTGTCAGGAATGACGGGATGGAGGATCGTTACAGCATTTTGATAAGGTTTGATAGTCAGGACTCGACAGATAATTTTTACAAGCATTTCAACGGGGGACGATTCTCGTCGCTTGAGGTGGAGGTTTGTCGTATTCTTTTCACGGTAGATGTTCAATACACTGGTTCGATAGAACATGCGCAAGCATCATCAGGAAGCTCAACTGAGCAGCCATCATGTCCAGTTTGTCTTGAGAGACTGGACCAAGATATGGAAGGAATTCTCACGACTATTTGCAATCATTCATTTCATTGTTCCTGCATTTCAAAATGGACAGATTCTTCATGTCCTGTATGTCGATATTGTCAGCAACAGCCTGAAAAGTCCAACTGTTTTGTTTGTCAAACTTCTGAGAATCTCTGGATGTGTGTTATCTGTGGCTTTGTTGGCTGTGGGAGGTATAAAGAAGGACATGCAATAAGGCATTGGAAAGAAACAGAACATTGTTATTCTCTTGAATTGGAAACACAACGTGTGTGGGATTATGCTGGTGACAATTATGTTCACCGCCTAATTCAATCCAAAACTGATGGGAAATTGGTTGAATTGAATTCACCGTTTATTGGATGTCAAAGTTGCGACTGCGTAGATTCTGAATACAGTGAGGCACTTTTGAACAGTAAAGTAGAAGCTATTGTTAATGAATACAATGAACTGCTTGCTACTCAACTTGAGAACCAAAAAGTGTATTTTGAGTCGTTACTTCAAGAAGTTAAAGAAGGAACTGAACGAGAAATTTCTAATGCTGTGGAGAAGGCTGTTCCACTAAAGAAATTGCAGGCCAAGCTGGACAGGTGCGTAAAAGAGAAGAAATTTCTTGACGATCTTAATGAAAACCTTTTAAAAAACCAGGAGATTTGGAAAGCAAAGATACTTGAATCTGAAGAGAGGGGGAGAAAGACTTTGAAACTAAAGGATGAAAAAATTAGCGACTTAGAAGAGCAGCTTGGGAATTTGATGATGTACCTTGAGGCTGGGAAGACGGTGGAGCAGCTATCAATATCAGATGAAATAAAGGATGGGACTGCCTTGCCCGTATCGGTGGAGCCACCACAGCCATCAGTACGGAATGAAATTGGTGACACCACTGTCACTTCTCCAGAAGTTTCCAATGAACGTCTAGAAGAGGGCTCAAAGACTAATAACAGGAGGAGGAGACGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
BRAP2 zf-RING_2 zf-UBP

Protein Analysis

516

Amino Acids

58.15

Weight (kDa)

5.07

Isoelectric Point (pI)

51.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013575)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26000 AT2G26000 AT2G26000
fragaria_vesca FvH4_6g29040
malus_domestica MD17G1218900.v1.1
prunus_persica Prupe.3G094400_v2.0.a1 Prupe.3G094400_v2.0.a1
pyrus_communis pycom17g22370
rosa_chinensis RchiOBHm_Chr2g0133711
rosa_laevigata RLG00000019358
rosa_multiflora Rmu_sc0013154.1_g000008
rosa_roxburghii Rroxscaffold_2G00110880
rosa_rugosa Rorug02G0313200
rosa_samantha Rh2AG366600 Rh2BG372300 Rh2CG349900 Rh2DG389200
rosa_wichuraiana Rw2G029830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1472
AatII GACGTC 1 cut(s) 210
Acc16I TGCGCA 1 cut(s) 525
AccB7I CCANNNNNTGG 1 cut(s) 595
AccII CGCG 1 cut(s) 189
AccIII TCCGGA 1 cut(s) 29
AciI CCGC 5 cut(s) 62, 187, 293, 343, 892
AclWI GGATC 1 cut(s) 378
AcsI RAATTY 5 cut(s) 603, 934, 1119, 1196, 1336
AcuI CTGAAG 1 cut(s) 1287
AcyI GRCGYC 2 cut(s) 195, 207
AdeI CACNNNGTG 1 cut(s) 857
AfaI GTAC 2 cut(s) 1349, 1449
AfiI CCNNNNNNNGG 3 cut(s) 243, 595, 947
AflIII ACRYGT 1 cut(s) 856
AjnI CCWGG 2 cut(s) 40, 1233
AjuI GAANNNNNNNTTGG 2 cut(s) 1314, 1346
AluBI AGCT 6 cut(s) 23, 543, 1016, 1171, 1330, 1378
AluI AGCT 6 cut(s) 23, 543, 1016, 1171, 1330, 1378
Alw21I GWGCWC 1 cut(s) 20
Alw26I GTCTC 3 cut(s) 155, 574, 1534
AlwI GGATC 1 cut(s) 378
Aor13HI TCCGGA 1 cut(s) 29
AoxI GGCC 2 cut(s) 123, 1164
ApeKI GCWGC 3 cut(s) 553, 1327, 1375
ApoI RAATTY 5 cut(s) 603, 934, 1119, 1196, 1336
Asp700I GAANNNNTTC 1 cut(s) 603
AspLEI GCGC 1 cut(s) 526
AspS9I GGNCC 1 cut(s) 586
AsuHPI GGTGA 4 cut(s) 881, 887, 930, 1475
AvaII GGWCC 1 cut(s) 586
BanII GRGCYC 1 cut(s) 1517
BbsI GAAGAC 1 cut(s) 1370
Bbv12I GWGCWC 1 cut(s) 20
BbvI GCAGC 3 cut(s) 565, 1339, 1387
BccI CCATC 6 cut(s) 113, 358, 565, 908, 1397, 1450
BcgI CGANNNNNNTGC 2 cut(s) 182, 216
BciT130I CCWGG 2 cut(s) 42, 1235
BcoDI GTCTC 3 cut(s) 155, 574, 1534
BfaI CTAG 2 cut(s) 150, 1505
BisI GCNGC 4 cut(s) 62, 554, 1328, 1376
BlsI GCNGC 4 cut(s) 63, 555, 1329, 1377
Bme1390I CCNGG 2 cut(s) 42, 1235
Bme18I GGWCC 1 cut(s) 586
BmgT120I GGNCC 1 cut(s) 586
BmiI GGNNCC 2 cut(s) 34, 1431
BmrFI CCNGG 2 cut(s) 42, 1235
BmsI GCATC 3 cut(s) 190, 330, 539
BpiI GAAGAC 1 cut(s) 1370
BplI GAGNNNNNCTC 2 cut(s) 7, 39
BpmI CTGGAG 2 cut(s) 58, 1467
BpuEI CTTGAG 4 cut(s) 479, 596, 1076, 1373
BsaHI GRCGYC 2 cut(s) 195, 207
BsaI GGTCTC 1 cut(s) 155
BsaJI CCNNGG 1 cut(s) 40
BsaWI WCCGGW 2 cut(s) 29, 54
Bsc4I CCNNNNNNNGG 3 cut(s) 243, 595, 947
Bse1I ACTGG 4 cut(s) 304, 511, 566, 588
BseAI TCCGGA 1 cut(s) 29
BseBI CCWGG 2 cut(s) 42, 1235
BseDI CCNNGG 1 cut(s) 40
BseGI GGATG 6 cut(s) 210, 369, 753, 956, 1306, 1408
BseLI CCNNNNNNNGG 3 cut(s) 243, 595, 947
BseMII CTCAG 2 cut(s) 540, 726
BseNI ACTGG 4 cut(s) 304, 511, 566, 588
BseXI GCAGC 3 cut(s) 565, 1339, 1387
BseYI CCCAGC 1 cut(s) 1358
Bsh1236I CGCG 1 cut(s) 189
Bsh1285I CGRYCG 1 cut(s) 59
BshFI GGCC 2 cut(s) 125, 1166
BsiEI CGRYCG 1 cut(s) 59
BsiHKAI GWGCWC 1 cut(s) 20
BsiSI CCGG 2 cut(s) 30, 55
BslFI GGGAC 2 cut(s) 456, 1420
BslI CCNNNNNNNGG 3 cut(s) 243, 595, 947
BsmAI GTCTC 3 cut(s) 155, 574, 1534
BsmBI CGTCTC 1 cut(s) 1534
BsmFI GGGAC 2 cut(s) 456, 1420
BsnI GGCC 2 cut(s) 125, 1166
Bso31I GGTCTC 1 cut(s) 155
Bsp1286I GDGCHC 2 cut(s) 20, 1517
Bsp13I TCCGGA 1 cut(s) 29
Bsp143I GATC 2 cut(s) 370, 1207
BspACI CCGC 5 cut(s) 62, 187, 293, 343, 892
BspANI GGCC 2 cut(s) 125, 1166
BspCNI CTCAG 2 cut(s) 541, 727
BspEI TCCGGA 1 cut(s) 29
BspFNI CGCG 1 cut(s) 189
BspLI GGNNCC 2 cut(s) 34, 1431
BspPI GGATC 1 cut(s) 378
BspQI GCTCTTC 1 cut(s) 1317
BspTNI GGTCTC 1 cut(s) 155
BsrI ACTGG 4 cut(s) 304, 511, 566, 588
BssECI CCNNGG 1 cut(s) 40
BssMI GATC 2 cut(s) 370, 1207
BssNI GRCGYC 2 cut(s) 195, 207
Bst2UI CCWGG 2 cut(s) 42, 1235
Bst4CI ACNGT 7 cut(s) 490, 716, 942, 986, 1004, 1369, 1474
Bst6I CTCTTC 5 cut(s) 142, 153, 1263, 1317, 1503
BstACI GRCGYC 2 cut(s) 195, 207
BstC8I GCNNGC 4 cut(s) 123, 528, 1044, 1164
BstDEI CTNAG 3 cut(s) 549, 735, 1318
BstF5I GGATG 6 cut(s) 210, 369, 753, 956, 1306, 1408
BstFNI CGCG 1 cut(s) 189
BstHHI GCGC 1 cut(s) 526
BstKTI GATC 2 cut(s) 373, 1210
BstMAI GTCTC 3 cut(s) 155, 574, 1534
BstMBI GATC 2 cut(s) 370, 1207
BstMCI CGRYCG 1 cut(s) 59
BstMWI GCNNNNNNNGC 4 cut(s) 84, 771, 1168, 1438
BstNI CCWGG 2 cut(s) 42, 1235
BstNSI RCATGY 2 cut(s) 524, 800
BstSCI CCNGG 2 cut(s) 40, 1233
BstUI CGCG 1 cut(s) 189
BstV1I GCAGC 3 cut(s) 565, 1339, 1387
BstV2I GAAGAC 1 cut(s) 1370
BstXI CCANNNNNNTGG 1 cut(s) 311
BsuRI GGCC 2 cut(s) 125, 1166
BtsCI GGATG 6 cut(s) 210, 369, 753, 956, 1306, 1408
BtsIMutI CAGTG 3 cut(s) 504, 991, 1470
Cac8I GCNNGC 4 cut(s) 123, 528, 1044, 1164
CfoI GCGC 1 cut(s) 526
Cfr13I GGNCC 1 cut(s) 586
CseI GACGC 1 cut(s) 203
Csp6I GTAC 2 cut(s) 1348, 1448
CspCI CAANNNNNGTGG 2 cut(s) 842, 877
CviAII CATG 5 cut(s) 283, 521, 561, 669, 797
CviQI GTAC 2 cut(s) 1348, 1448
DdeI CTNAG 3 cut(s) 549, 735, 1318
DpnI GATC 2 cut(s) 372, 1209
DpnII GATC 2 cut(s) 370, 1207
DraI TTTAAA 1 cut(s) 1227
DraIII CACNNNGTG 1 cut(s) 857
DrdI GACNNNNNNGTC 1 cut(s) 1472
DseDI GACNNNNNNGTC 1 cut(s) 1472
Eam1104I CTCTTC 5 cut(s) 142, 153, 1263, 1317, 1503
EarI CTCTTC 5 cut(s) 142, 153, 1263, 1317, 1503
Eco147I AGGCCT 1 cut(s) 125
Eco24I GRGCYC 1 cut(s) 1517
Eco31I GGTCTC 1 cut(s) 155
Eco47I GGWCC 1 cut(s) 586
Eco57I CTGAAG 1 cut(s) 1287
EcoRI GAATTC 2 cut(s) 603, 934
EcoRII CCWGG 2 cut(s) 40, 1233
EcoT38I GRGCYC 1 cut(s) 1517
Esp3I CGTCTC 1 cut(s) 1534
FaeI CATG 5 cut(s) 286, 524, 564, 672, 800
FalI AAGNNNNNCTT 2 cut(s) 1314, 1346
FaqI GGGAC 2 cut(s) 456, 1420
FatI CATG 5 cut(s) 282, 520, 560, 668, 796
FauNDI CATATG 1 cut(s) 289
Fnu4HI GCNGC 4 cut(s) 62, 554, 1328, 1376
FokI GGATG 6 cut(s) 197, 376, 760, 963, 1313, 1415
FriOI GRGCYC 1 cut(s) 1517
Fsp4HI GCNGC 4 cut(s) 62, 554, 1328, 1376
FspBI CTAG 2 cut(s) 150, 1505
FspI TGCGCA 1 cut(s) 525
GlaI GCGC 1 cut(s) 525
GluI GCNGC 4 cut(s) 62, 554, 1328, 1376
GsaI CCCAGC 1 cut(s) 1362
GsuI CTGGAG 2 cut(s) 58, 1467
HaeIII GGCC 2 cut(s) 125, 1166
HapII CCGG 2 cut(s) 30, 55
HgaI GACGC 1 cut(s) 203
HhaI GCGC 1 cut(s) 526
Hin1I GRCGYC 2 cut(s) 195, 207
Hin1II CATG 5 cut(s) 286, 524, 564, 672, 800
Hin6I GCGC 1 cut(s) 524
HinP1I GCGC 1 cut(s) 524
HpaII CCGG 2 cut(s) 30, 55
HphI GGTGA 4 cut(s) 881, 887, 930, 1475
Hpy166II GTNNAC 1 cut(s) 889
Hpy188I TCNGA 5 cut(s) 276, 736, 979, 1267, 1390
Hpy8I GTNNAC 1 cut(s) 889
Hpy99I CGWCG 4 cut(s) 194, 197, 209, 457
HpyAV CCTTC 6 cut(s) 75, 237, 593, 785, 1097, 1132
HpyCH4III ACNGT 7 cut(s) 490, 716, 942, 986, 1004, 1369, 1474
HpyCH4IV ACGT 3 cut(s) 207, 856, 1501
HpyCH4V TGCA 4 cut(s) 621, 645, 800, 1162
HpyF10VI GCNNNNNNNGC 4 cut(s) 84, 771, 1168, 1438
HpyF3I CTNAG 3 cut(s) 549, 735, 1318
HpySE526I ACGT 3 cut(s) 207, 856, 1501
Hsp92I GRCGYC 2 cut(s) 195, 207
Hsp92II CATG 5 cut(s) 286, 524, 564, 672, 800
HspAI GCGC 1 cut(s) 524
Kpn2I TCCGGA 1 cut(s) 29
Kzo9I GATC 2 cut(s) 370, 1207
LguI GCTCTTC 1 cut(s) 1317
LmnI GCTCC 3 cut(s) 32, 1372, 1429
Lsp1109I GCAGC 3 cut(s) 565, 1339, 1387
LweI GCATC 3 cut(s) 190, 330, 539
MaeI CTAG 2 cut(s) 150, 1505
MaeII ACGT 3 cut(s) 207, 856, 1501
MaeIII GTNAC 6 cut(s) 3, 374, 875, 1083, 1463, 1474
MalI GATC 2 cut(s) 372, 1209
MboI GATC 2 cut(s) 370, 1207
MboII GAAGA 8 cut(s) 129, 170, 657, 1204, 1280, 1334, 1375, 1520
MhlI GDGCHC 2 cut(s) 20, 1517
MlyI GAGTC 2 cut(s) 401, 1088
MmeI TCCRAC 3 cut(s) 135, 220, 735
MroI TCCGGA 1 cut(s) 29
MroXI GAANNNNTTC 1 cut(s) 603
MseI TTAA 4 cut(s) 1023, 1098, 1212, 1226
MspI CCGG 2 cut(s) 30, 55
MspR9I CCNGG 2 cut(s) 42, 1235
MvaI CCWGG 2 cut(s) 42, 1235
MvnI CGCG 1 cut(s) 189
MwoI GCNNNNNNNGC 4 cut(s) 84, 771, 1168, 1438
NdeI CATATG 1 cut(s) 289
NdeII GATC 2 cut(s) 370, 1207
NlaIII CATG 5 cut(s) 286, 524, 564, 672, 800
NlaIV GGNNCC 2 cut(s) 34, 1431
NmuCI GTSAC 4 cut(s) 3, 875, 1463, 1474
NsbI TGCGCA 1 cut(s) 525
NspI RCATGY 2 cut(s) 524, 800
PceI AGGCCT 1 cut(s) 125
PciSI GCTCTTC 1 cut(s) 1317
PcsI WCGNNNNNNNCGW 1 cut(s) 201
PdmI GAANNNNTTC 1 cut(s) 603
PfeI GAWTC 8 cut(s) 89, 153, 277, 447, 662, 739, 974, 1262
PflMI CCANNNNNTGG 1 cut(s) 595
PkrI GCNGC 4 cut(s) 63, 555, 1329, 1377
PleI GAGTC 2 cut(s) 401, 1087
PpsI GAGTC 2 cut(s) 401, 1087
Psp6I CCWGG 2 cut(s) 40, 1233
PspFI CCCAGC 1 cut(s) 1358
PspGI CCWGG 2 cut(s) 40, 1233
PspN4I GGNNCC 2 cut(s) 34, 1431
PspPI GGNCC 1 cut(s) 586
RsaI GTAC 2 cut(s) 1349, 1449
RsaNI GTAC 2 cut(s) 1348, 1448
SapI GCTCTTC 1 cut(s) 1317
SaqAI TTAA 4 cut(s) 1023, 1098, 1212, 1226
SatI GCNGC 4 cut(s) 62, 554, 1328, 1376
Sau3AI GATC 2 cut(s) 370, 1207
Sau96I GGNCC 1 cut(s) 586
SchI GAGTC 2 cut(s) 401, 1088
ScrFI CCNGG 2 cut(s) 42, 1235
SduI GDGCHC 2 cut(s) 20, 1517
SfaNI GCATC 3 cut(s) 190, 330, 539
SinI GGWCC 1 cut(s) 586
SmlI CTYRAG 4 cut(s) 458, 575, 1055, 1352
SmoI CTYRAG 4 cut(s) 458, 575, 1055, 1352
SseBI AGGCCT 1 cut(s) 125
SsiI CCGC 5 cut(s) 62, 187, 293, 343, 892
SspMI CTAG 2 cut(s) 150, 1505
StuI AGGCCT 1 cut(s) 125
StyD4I CCNGG 2 cut(s) 40, 1233
TaaI ACNGT 7 cut(s) 490, 716, 942, 986, 1004, 1369, 1474
TaiI ACGT 3 cut(s) 210, 859, 1504
TaqI TCGA 5 cut(s) 14, 156, 410, 512, 682
TauI GCSGC 1 cut(s) 64
TfiI GAWTC 8 cut(s) 89, 153, 277, 447, 662, 739, 974, 1262
Tru1I TTAA 4 cut(s) 1023, 1098, 1212, 1226
Tru9I TTAA 4 cut(s) 1023, 1098, 1212, 1226
TscAI CASTG 3 cut(s) 511, 991, 1477
TseFI GTSAC 4 cut(s) 3, 875, 1463, 1474
TseI GCWGC 3 cut(s) 553, 1327, 1375
Tsp45I GTSAC 4 cut(s) 3, 875, 1463, 1474
TspGWI ACGGA 1 cut(s) 1465
TspRI CASTG 3 cut(s) 511, 991, 1477
Van91I CCANNNNNTGG 1 cut(s) 595
VpaK11BI GGWCC 1 cut(s) 586
XapI RAATTY 5 cut(s) 603, 934, 1119, 1196, 1336
XbaI TCTAGA 1 cut(s) 1504
XceI RCATGY 2 cut(s) 524, 800
XmnI GAANNNNTTC 1 cut(s) 603
XspI CTAG 2 cut(s) 150, 1505
ZraI GACGTC 1 cut(s) 208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.