FvH4_6g29370

VQ motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0013553
Physical Location & Seq
Forward (+)
22652259 .. 22653837
1579 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g29370.t1

Sequence Viewer

Length: 588 bp
ATGAACACTCACCAACCTACTTACGGATCCGACCCGGCTTCCCCCAACACCACCTACGTCCAAGCCGACCCATCAAGCTTCCGGGCCGTCGTCCAGAAGCTCACCGGAGCAACGGAGGACCCCTCAGCCCAGAAGCTCCCTCTGACCCTCCCCGCACGCTACACCAACCCCAAACCCGCCGCCGAAGTGGGGCCCCGACGGCCCGCCTTCAAGCTCCACGAGAGGAGACAAAGCACCAAGAAGCTCGAGCTCCACCTCACTACCTCCACAACAGTACTACACCCTTCGTCATCATCGTCGTGTGGACCCCAGCAGCTGGCTCGGCCGGTGATGATGTCACCAGTTTCTCCGTTTGAGTTTCTGGCACGTGGGAGCCCCAGGACGCCGGTGTCGCCGTGCGAGGTGGTGGACGAGGAAGAGGCGGAGCGAGCCATTATCGCAGAGAAAGGGTTCTATATGCACCAACCAAGTCCGCTAAGTACACCGCGAGGGCCTGATCATCACCCACCTGAGCTTTTGCCTTTGTTTCCTTTGCATTCTCCTCGAGACATCACTCAGACTCATCAACCCTCCACCTCTATTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VQ

Protein Analysis

196

Amino Acids

21.35

Weight (kDa)

7.89

Isoelectric Point (pI)

68.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VQ PF05678 15 - 41 1.9e-12 VQ motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013553)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G80450
fragaria_vesca FvH4_6g29370
malus_domestica MD08G1052300.v1.1 MD15G1043200.v1.1 MD17G1215500.v1.1
prunus_persica Prupe.1G396600_v2.0.a1 Prupe.3G091700_v2.0.a1
pyrus_communis pycom08g04360 pycom17g21990
rosa_chinensis RchiOBHm_Chr2g0134771
rosa_laevigata RLG00000019420
rosa_multiflora Rmu_sc0000718.1_g000019
rosa_roxburghii Rroxscaffold_2G00109770
rosa_samantha Rh2AG373800 Rh2BG377500 Rh2CG355600 Rh2DG394500
rosa_wichuraiana Rw2G030280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 388
AccB7I CCANNNNNTGG 1 cut(s) 316
AccII CGCG 1 cut(s) 487
AciI CCGC 7 cut(s) 153, 177, 180, 204, 422, 473, 485
AclWI GGATC 2 cut(s) 21, 34
AcoI YGGCCR 1 cut(s) 323
AcvI CACGTG 1 cut(s) 368
AcyI GRCGYC 1 cut(s) 383
AfaI GTAC 2 cut(s) 276, 481
AfiI CCNNNNNNNGG 3 cut(s) 23, 189, 316
AgsI TTSAA 1 cut(s) 211
AjnI CCWGG 1 cut(s) 377
AluBI AGCT 8 cut(s) 78, 100, 136, 214, 244, 250, 316, 514
AluI AGCT 8 cut(s) 78, 100, 136, 214, 244, 250, 316, 514
Alw21I GWGCWC 1 cut(s) 252
Alw26I GTCTC 2 cut(s) 220, 540
AlwI GGATC 2 cut(s) 21, 34
AlwNI CAGNNNCTG 1 cut(s) 316
Ama87I CYCGRG 2 cut(s) 245, 543
AoxI GGCC 5 cut(s) 84, 191, 200, 323, 491
ApaI GGGCCC 1 cut(s) 195
ApeKI GCWGC 1 cut(s) 313
Asp700I GAANNNNTTC 1 cut(s) 449
AspS9I GGNCC 7 cut(s) 84, 118, 191, 192, 201, 305, 491
AsuC2I CCSGG 2 cut(s) 35, 83
AsuHPI GGTGA 4 cut(s) 94, 330, 340, 494
AvaI CYCGRG 2 cut(s) 245, 543
AvaII GGWCC 2 cut(s) 118, 305
BaeGI GKGCMC 1 cut(s) 195
BamHI GGATCC 1 cut(s) 26
BanII GRGCYC 3 cut(s) 195, 252, 377
BauI CACGAG 1 cut(s) 218
BbrPI CACGTG 1 cut(s) 368
Bbv12I GWGCWC 1 cut(s) 252
BbvCI CCTCAGC 1 cut(s) 124
BbvI GCAGC 1 cut(s) 325
BccI CCATC 1 cut(s) 79
BceAI ACGGC 3 cut(s) 71, 215, 379
BcgI CGANNNNNNTGC 4 cut(s) 302, 336, 524, 558
BciT130I CCWGG 1 cut(s) 379
BclI TGATCA 1 cut(s) 496
BcnI CCSGG 2 cut(s) 35, 83
BcoDI GTCTC 2 cut(s) 220, 540
BglI GCCNNNNNGGC 1 cut(s) 199
BisI GCNGC 2 cut(s) 180, 314
BlsI GCNGC 2 cut(s) 181, 315
BmcAI AGTACT 1 cut(s) 276
Bme1390I CCNGG 3 cut(s) 35, 83, 379
Bme18I GGWCC 2 cut(s) 118, 305
BmeT110I CYCGRG 2 cut(s) 245, 543
BmgT120I GGNCC 7 cut(s) 84, 118, 191, 192, 201, 305, 491
BmiI GGNNCC 7 cut(s) 28, 120, 192, 193, 194, 307, 374
BmrFI CCNGG 3 cut(s) 35, 83, 379
BplI GAGNNNNNCTC 2 cut(s) 107, 139
Bpu10I CCTNAGC 2 cut(s) 124, 510
BpuMI CCSGG 2 cut(s) 35, 83
BsaAI YACGTR 1 cut(s) 368
BsaHI GRCGYC 1 cut(s) 383
BsaJI CCNNGG 1 cut(s) 377
BsaWI WCCGGW 1 cut(s) 104
Bsc4I CCNNNNNNNGG 3 cut(s) 23, 189, 316
Bse118I RCCGGY 2 cut(s) 325, 385
Bse1I ACTGG 1 cut(s) 341
BseBI CCWGG 1 cut(s) 379
BseDI CCNNGG 1 cut(s) 377
BseLI CCNNNNNNNGG 3 cut(s) 23, 189, 316
BseMII CTCAG 3 cut(s) 138, 501, 569
BseNI ACTGG 1 cut(s) 341
BseRI GAGGAG 2 cut(s) 238, 531
BseSI GKGCMC 1 cut(s) 195
BseX3I CGGCCG 1 cut(s) 323
BseXI GCAGC 1 cut(s) 325
BseYI CCCAGC 1 cut(s) 309
Bsh1236I CGCG 1 cut(s) 487
Bsh1285I CGRYCG 1 cut(s) 326
BshFI GGCC 5 cut(s) 86, 193, 202, 325, 493
BsiEI CGRYCG 1 cut(s) 326
BsiHKAI GWGCWC 1 cut(s) 252
BsiHKCI CYCGRG 2 cut(s) 245, 543
BsiSI CCGG 5 cut(s) 35, 82, 105, 326, 386
BslI CCNNNNNNNGG 3 cut(s) 23, 189, 316
BsmAI GTCTC 2 cut(s) 220, 540
BsmI GAATGC 1 cut(s) 535
BsnI GGCC 5 cut(s) 86, 193, 202, 325, 493
BsoBI CYCGRG 2 cut(s) 245, 543
Bsp120I GGGCCC 1 cut(s) 191
Bsp1286I GDGCHC 3 cut(s) 195, 252, 377
Bsp143I GATC 2 cut(s) 26, 496
BspACI CCGC 7 cut(s) 153, 177, 180, 204, 422, 473, 485
BspANI GGCC 5 cut(s) 86, 193, 202, 325, 493
BspCNI CTCAG 3 cut(s) 137, 502, 568
BspFNI CGCG 1 cut(s) 487
BspLI GGNNCC 7 cut(s) 28, 120, 192, 193, 194, 307, 374
BspPI GGATC 2 cut(s) 21, 34
BsrFI RCCGGY 2 cut(s) 325, 385
BsrI ACTGG 1 cut(s) 341
BssAI RCCGGY 2 cut(s) 325, 385
BssECI CCNNGG 1 cut(s) 377
BssMI GATC 2 cut(s) 26, 496
BssNI GRCGYC 1 cut(s) 383
BssSI CACGAG 1 cut(s) 218
Bst2BI CACGAG 1 cut(s) 218
Bst2UI CCWGG 1 cut(s) 379
Bst4CI ACNGT 1 cut(s) 274
Bst6I CTCTTC 1 cut(s) 411
BstACI GRCGYC 1 cut(s) 383
BstBAI YACGTR 1 cut(s) 368
BstC8I GCNNGC 4 cut(s) 157, 204, 318, 429
BstDEI CTNAG 4 cut(s) 124, 476, 510, 555
BstFNI CGCG 1 cut(s) 487
BstKTI GATC 2 cut(s) 29, 499
BstMAI GTCTC 2 cut(s) 220, 540
BstMBI GATC 2 cut(s) 26, 496
BstMCI CGRYCG 1 cut(s) 326
BstMWI GCNNNNNNNGC 5 cut(s) 199, 322, 391, 428, 437
BstNI CCWGG 1 cut(s) 379
BstSCI CCNGG 3 cut(s) 33, 81, 377
BstSLI GKGCMC 1 cut(s) 195
BstUI CGCG 1 cut(s) 487
BstV1I GCAGC 1 cut(s) 325
BstX2I RGATCY 1 cut(s) 26
BstYI RGATCY 1 cut(s) 26
BstZI CGGCCG 1 cut(s) 323
BsuRI GGCC 5 cut(s) 86, 193, 202, 325, 493
Cac8I GCNNGC 4 cut(s) 157, 204, 318, 429
CaiI CAGNNNCTG 1 cut(s) 316
Cfr10I RCCGGY 2 cut(s) 325, 385
Cfr13I GGNCC 7 cut(s) 84, 118, 191, 192, 201, 305, 491
CseI GACGC 1 cut(s) 391
Csp6I GTAC 2 cut(s) 275, 480
CviQI GTAC 2 cut(s) 275, 480
DdeI CTNAG 4 cut(s) 124, 476, 510, 555
DpnI GATC 2 cut(s) 28, 498
DpnII GATC 2 cut(s) 26, 496
DrdI GACNNNNNNGTC 1 cut(s) 388
DseDI GACNNNNNNGTC 1 cut(s) 388
EaeI YGGCCR 1 cut(s) 323
EagI CGGCCG 1 cut(s) 323
Eam1104I CTCTTC 1 cut(s) 411
EarI CTCTTC 1 cut(s) 411
EciI GGCGGA 1 cut(s) 437
Ecl136II GAGCTC 1 cut(s) 250
EclXI CGGCCG 1 cut(s) 323
Eco24I GRGCYC 3 cut(s) 195, 252, 377
Eco47I GGWCC 2 cut(s) 118, 305
Eco52I CGGCCG 1 cut(s) 323
Eco53kI GAGCTC 1 cut(s) 250
Eco72I CACGTG 1 cut(s) 368
Eco88I CYCGRG 2 cut(s) 245, 543
EcoICRI GAGCTC 1 cut(s) 250
EcoO109I RGGNCCY 4 cut(s) 118, 191, 192, 491
EcoRII CCWGG 1 cut(s) 377
EcoT38I GRGCYC 3 cut(s) 195, 252, 377
FaiI YATR 2 cut(s) 456, 458
FauI CCCGC 3 cut(s) 160, 184, 211
FbaI TGATCA 1 cut(s) 496
Fnu4HI GCNGC 2 cut(s) 180, 314
FriOI GRGCYC 3 cut(s) 195, 252, 377
Fsp4HI GCNGC 2 cut(s) 180, 314
GluI GCNGC 2 cut(s) 180, 314
GsaI CCCAGC 1 cut(s) 313
HaeIII GGCC 5 cut(s) 86, 193, 202, 325, 493
HapII CCGG 5 cut(s) 35, 82, 105, 326, 386
HgaI GACGC 1 cut(s) 391
Hin1I GRCGYC 1 cut(s) 383
HindIII AAGCTT 1 cut(s) 76
HinfI GANTC 1 cut(s) 559
HpaII CCGG 5 cut(s) 35, 82, 105, 326, 386
HphI GGTGA 4 cut(s) 94, 330, 340, 494
Hpy166II GTNNAC 3 cut(s) 305, 409, 482
Hpy188I TCNGA 3 cut(s) 31, 144, 558
Hpy188III TCNNGA 2 cut(s) 94, 545
Hpy8I GTNNAC 3 cut(s) 305, 409, 482
Hpy99I CGWCG 3 cut(s) 92, 201, 301
HpyAV CCTTC 2 cut(s) 217, 294
HpyCH4III ACNGT 1 cut(s) 274
HpyCH4IV ACGT 2 cut(s) 57, 367
HpyCH4V TGCA 2 cut(s) 460, 535
HpyF10VI GCNNNNNNNGC 5 cut(s) 199, 322, 391, 428, 437
HpyF3I CTNAG 4 cut(s) 124, 476, 510, 555
HpySE526I ACGT 2 cut(s) 57, 367
Hsp92I GRCGYC 1 cut(s) 383
Ksp22I TGATCA 1 cut(s) 496
Kzo9I GATC 2 cut(s) 26, 496
LmnI GCTCC 6 cut(s) 107, 141, 219, 255, 372, 424
Lsp1109I GCAGC 1 cut(s) 325
MaeII ACGT 2 cut(s) 57, 367
MaeIII GTNAC 1 cut(s) 336
MalI GATC 2 cut(s) 28, 498
MboI GATC 2 cut(s) 26, 496
MboII GAAGA 1 cut(s) 428
MflI RGATCY 1 cut(s) 26
MhlI GDGCHC 3 cut(s) 195, 252, 377
MlyI GAGTC 1 cut(s) 553
MmeI TCCRAC 1 cut(s) 54
MroXI GAANNNNTTC 1 cut(s) 449
MseI TTAA 1 cut(s) 586
MslI CAYNNNNRTG 1 cut(s) 298
MspA1I CMGCKG 1 cut(s) 316
MspI CCGG 5 cut(s) 35, 82, 105, 326, 386
MspR9I CCNGG 3 cut(s) 35, 83, 379
Mva1269I GAATGC 1 cut(s) 535
MvaI CCWGG 1 cut(s) 379
MvnI CGCG 1 cut(s) 487
MwoI GCNNNNNNNGC 5 cut(s) 199, 322, 391, 428, 437
NciI CCSGG 2 cut(s) 35, 83
NdeII GATC 2 cut(s) 26, 496
NlaIV GGNNCC 7 cut(s) 28, 120, 192, 193, 194, 307, 374
NmeAIII GCCGAG 1 cut(s) 301
NmuCI GTSAC 1 cut(s) 336
PaeR7I CTCGAG 2 cut(s) 245, 543
PcsI WCGNNNNNNNCGW 2 cut(s) 63, 293
PctI GAATGC 1 cut(s) 535
PdmI GAANNNNTTC 1 cut(s) 449
PflMI CCANNNNNTGG 1 cut(s) 316
PkrI GCNGC 2 cut(s) 181, 315
PleI GAGTC 1 cut(s) 553
PmaCI CACGTG 1 cut(s) 368
PmlI CACGTG 1 cut(s) 368
PpsI GAGTC 1 cut(s) 553
Ppu21I YACGTR 1 cut(s) 368
PpuMI RGGWCCY 1 cut(s) 118
Psp124BI GAGCTC 1 cut(s) 252
Psp5II RGGWCCY 1 cut(s) 118
Psp6I CCWGG 1 cut(s) 377
PspCI CACGTG 1 cut(s) 368
PspFI CCCAGC 1 cut(s) 309
PspGI CCWGG 1 cut(s) 377
PspN4I GGNNCC 7 cut(s) 28, 120, 192, 193, 194, 307, 374
PspOMI GGGCCC 1 cut(s) 191
PspPI GGNCC 7 cut(s) 84, 118, 191, 192, 201, 305, 491
PspPPI RGGWCCY 1 cut(s) 118
PspXI VCTCGAGB 1 cut(s) 245
PstNI CAGNNNCTG 1 cut(s) 316
PsuI RGATCY 1 cut(s) 26
PvuII CAGCTG 1 cut(s) 316
RsaI GTAC 2 cut(s) 276, 481
RsaNI GTAC 2 cut(s) 275, 480
RseI CAYNNNNRTG 1 cut(s) 298
SacI GAGCTC 1 cut(s) 252
SaqAI TTAA 1 cut(s) 586
SatI GCNGC 2 cut(s) 180, 314
Sau3AI GATC 2 cut(s) 26, 496
Sau96I GGNCC 7 cut(s) 84, 118, 191, 192, 201, 305, 491
ScaI AGTACT 1 cut(s) 276
SchI GAGTC 1 cut(s) 553
ScrFI CCNGG 3 cut(s) 35, 83, 379
SduI GDGCHC 3 cut(s) 195, 252, 377
SfiI GGCCNNNNNGGCC 1 cut(s) 199
Sfr274I CTCGAG 2 cut(s) 245, 543
SgrAI CRCCGGYG 1 cut(s) 385
SinI GGWCC 2 cut(s) 118, 305
SlaI CTCGAG 2 cut(s) 245, 543
SmiMI CAYNNNNRTG 1 cut(s) 298
SmlI CTYRAG 2 cut(s) 245, 543
SmoI CTYRAG 2 cut(s) 245, 543
SsiI CCGC 7 cut(s) 153, 177, 180, 204, 422, 473, 485
SstI GAGCTC 1 cut(s) 252
StyD4I CCNGG 3 cut(s) 33, 81, 377
TaaI ACNGT 1 cut(s) 274
TaiI ACGT 2 cut(s) 60, 370
TaqI TCGA 2 cut(s) 246, 544
TatI WGTACW 2 cut(s) 274, 479
TauI GCSGC 1 cut(s) 182
Tru1I TTAA 1 cut(s) 586
Tru9I TTAA 1 cut(s) 586
TseFI GTSAC 1 cut(s) 336
TseI GCWGC 1 cut(s) 313
Tsp45I GTSAC 1 cut(s) 336
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 3 cut(s) 39, 128, 339
Van91I CCANNNNNTGG 1 cut(s) 316
VpaK11BI GGWCC 2 cut(s) 118, 305
XhoI CTCGAG 2 cut(s) 245, 543
XmnI GAANNNNTTC 1 cut(s) 449
ZrmI AGTACT 1 cut(s) 276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.