MD17G1215500.v1.1

VQ motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0013553
Physical Location & Seq
Reverse (-)
26252398 .. 26252997
600 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1215500.v1.1.491

Sequence Viewer

Length: 600 bp
ATGGTCAACTCACCCACTTACGGGTCCGACCCCAACTCCCCCAACACAACTTACGTCCAAGCCGACCCCACAACTTTCCGGGCCGTCGTCCAAAGACTGACCGGAGCACCCGAAGACCCATCTGCCCCAAAGCTACCCCTCACCCTCCCCGCCCGCTACTCCACCCCCAAACCCCCCACCGCCACCGAAATGGGGCCCCGGCGACCGGCTTTCAAGCTCCACGAGCGCAGACACGCCACCAAGAAGCTCGAGCTCAGCCTCAACCTCAACACCGCCGCCGCAGCAACCCCATCATCCTCCTCGTGTGGGCCCTGCGGCAGGCTGCCCAGAGTGGGAGCAGGGTTTATGGGTTTTGGGAGCCATGAGATGGTGATGGCGTCGCCCGTTTCGACCCTGGATTTTCTGGGACGTGGGAGCCCGAGGACGCCGCCGGCGTCTCTGTGTGAAGAGGAAGATCGAGCCATTGCTGGGAAAGGGTTCTATTTGCACCCAAGTCCGCTAAGTGCACCCCGAGGGATGACTGACCACACTCCTGAGCTTTTGCCTCTGTTTCCTCTGCAATCTCCTAGAGATAGTCAAAACTCTTGTTCTTCCACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VQ

Protein Analysis

200

Amino Acids

21.2

Weight (kDa)

9.02

Isoelectric Point (pI)

59.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VQ PF05678 14 - 40 9.8e-13 VQ motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013553)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G80450
fragaria_vesca FvH4_6g29370
malus_domestica MD08G1052300.v1.1 MD15G1043200.v1.1 MD17G1215500.v1.1
prunus_persica Prupe.1G396600_v2.0.a1 Prupe.3G091700_v2.0.a1
pyrus_communis pycom08g04360 pycom17g21990
rosa_chinensis RchiOBHm_Chr2g0134771
rosa_laevigata RLG00000019420
rosa_multiflora Rmu_sc0000718.1_g000019
rosa_roxburghii Rroxscaffold_2G00109770
rosa_samantha Rh2AG373800 Rh2BG377500 Rh2CG355600 Rh2DG394500
rosa_wichuraiana Rw2G030280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 367
AciI CCGC 9 cut(s) 150, 154, 180, 273, 276, 279, 315, 428, 497
AcyI GRCGYC 3 cut(s) 377, 425, 434
AfiI CCNNNNNNNGG 9 cut(s) 20, 21, 192, 205, 306, 318, 332, 367, 468
AgsI TTSAA 1 cut(s) 214
AjiI CACGTC 1 cut(s) 410
AjnI CCWGG 1 cut(s) 393
AluBI AGCT 5 cut(s) 133, 217, 247, 253, 538
AluI AGCT 5 cut(s) 133, 217, 247, 253, 538
Alw21I GWGCWC 3 cut(s) 109, 255, 508
Alw26I GTCTC 1 cut(s) 441
Alw44I GTGCAC 1 cut(s) 504
Ama87I CYCGRG 3 cut(s) 248, 418, 510
AoxI GGCC 3 cut(s) 81, 194, 308
ApaI GGGCCC 2 cut(s) 198, 312
ApaLI GTGCAC 1 cut(s) 504
ApeKI GCWGC 2 cut(s) 281, 322
Asp700I GAANNNNTTC 1 cut(s) 476
AspLEI GCGC 1 cut(s) 228
AspS9I GGNCC 6 cut(s) 24, 81, 194, 195, 308, 309
AsuC2I CCSGG 2 cut(s) 80, 199
AsuHPI GGTGA 3 cut(s) 3, 133, 382
AvaI CYCGRG 3 cut(s) 248, 418, 510
AvaII GGWCC 1 cut(s) 24
BaeGI GKGCMC 3 cut(s) 198, 312, 508
BanII GRGCYC 4 cut(s) 198, 255, 312, 419
BauI CACGAG 2 cut(s) 221, 301
BbsI GAAGAC 1 cut(s) 120
Bbv12I GWGCWC 3 cut(s) 109, 255, 508
BbvI GCAGC 2 cut(s) 293, 309
BccI CCATC 4 cut(s) 127, 298, 361, 367
BceAI ACGGC 1 cut(s) 68
BciT130I CCWGG 1 cut(s) 395
BcnI CCSGG 2 cut(s) 80, 199
BcoDI GTCTC 1 cut(s) 441
BfaI CTAG 1 cut(s) 567
BisI GCNGC 6 cut(s) 276, 279, 282, 316, 323, 428
BlpI GCTNAGC 1 cut(s) 254
BlsI GCNGC 6 cut(s) 277, 280, 283, 317, 324, 429
Bme1390I CCNGG 3 cut(s) 80, 199, 395
Bme18I GGWCC 1 cut(s) 24
BmeT110I CYCGRG 3 cut(s) 248, 418, 510
BmgBI CACGTC 1 cut(s) 410
BmgT120I GGNCC 6 cut(s) 24, 81, 194, 195, 308, 309
BmiI GGNNCC 7 cut(s) 25, 195, 196, 197, 310, 359, 416
BmrFI CCNGG 3 cut(s) 80, 199, 395
BpiI GAAGAC 1 cut(s) 120
Bpu10I CCTNAGC 1 cut(s) 534
Bpu1102I GCTNAGC 1 cut(s) 254
BpuMI CCSGG 2 cut(s) 80, 199
BsaHI GRCGYC 3 cut(s) 377, 425, 434
BsaJI CCNNGG 4 cut(s) 197, 393, 419, 511
BsaWI WCCGGW 1 cut(s) 101
BsaXI ACNNNNNCTCC 2 cut(s) 20, 50
Bsc4I CCNNNNNNNGG 9 cut(s) 20, 21, 192, 205, 306, 318, 332, 367, 468
Bse118I RCCGGY 2 cut(s) 205, 430
Bse3DI GCAATG 1 cut(s) 462
BseBI CCWGG 1 cut(s) 395
BseDI CCNNGG 4 cut(s) 197, 393, 419, 511
BseGI GGATG 2 cut(s) 293, 522
BseLI CCNNNNNNNGG 9 cut(s) 20, 21, 192, 205, 306, 318, 332, 367, 468
BseMI GCAATG 1 cut(s) 462
BseMII CTCAG 2 cut(s) 268, 525
BseRI GAGGAG 1 cut(s) 289
BseSI GKGCMC 3 cut(s) 198, 312, 508
BseXI GCAGC 2 cut(s) 293, 309
BseYI CCCAGC 1 cut(s) 467
Bsh1285I CGRYCG 1 cut(s) 206
BshFI GGCC 3 cut(s) 83, 196, 310
BsiEI CGRYCG 1 cut(s) 206
BsiHKAI GWGCWC 3 cut(s) 109, 255, 508
BsiHKCI CYCGRG 3 cut(s) 248, 418, 510
BsiSI CCGG 5 cut(s) 79, 102, 199, 206, 431
BslFI GGGAC 1 cut(s) 420
BslI CCNNNNNNNGG 9 cut(s) 20, 21, 192, 205, 306, 318, 332, 367, 468
BsmAI GTCTC 1 cut(s) 441
BsmBI CGTCTC 1 cut(s) 441
BsmFI GGGAC 1 cut(s) 420
BsnI GGCC 3 cut(s) 83, 196, 310
BsoBI CYCGRG 3 cut(s) 248, 418, 510
Bsp120I GGGCCC 2 cut(s) 194, 308
Bsp1286I GDGCHC 6 cut(s) 109, 198, 255, 312, 419, 508
Bsp143I GATC 1 cut(s) 454
Bsp1720I GCTNAGC 1 cut(s) 254
BspACI CCGC 9 cut(s) 150, 154, 180, 273, 276, 279, 315, 428, 497
BspANI GGCC 3 cut(s) 83, 196, 310
BspCNI CTCAG 2 cut(s) 267, 526
BspLI GGNNCC 7 cut(s) 25, 195, 196, 197, 310, 359, 416
BsrDI GCAATG 1 cut(s) 462
BsrFI RCCGGY 2 cut(s) 205, 430
BssAI RCCGGY 2 cut(s) 205, 430
BssECI CCNNGG 4 cut(s) 197, 393, 419, 511
BssMI GATC 1 cut(s) 454
BssNI GRCGYC 3 cut(s) 377, 425, 434
BssSI CACGAG 2 cut(s) 221, 301
Bst2BI CACGAG 2 cut(s) 221, 301
Bst2UI CCWGG 1 cut(s) 395
Bst6I CTCTTC 1 cut(s) 441
BstACI GRCGYC 3 cut(s) 377, 425, 434
BstC8I GCNNGC 3 cut(s) 154, 320, 432
BstDEI CTNAG 3 cut(s) 254, 500, 534
BstENI CCTNNNNNAGG 1 cut(s) 316
BstF5I GGATG 2 cut(s) 293, 522
BstHHI GCGC 1 cut(s) 228
BstKTI GATC 1 cut(s) 457
BstMAI GTCTC 1 cut(s) 441
BstMBI GATC 1 cut(s) 454
BstMCI CGRYCG 1 cut(s) 206
BstMWI GCNNNNNNNGC 2 cut(s) 223, 281
BstNI CCWGG 1 cut(s) 395
BstSCI CCNGG 3 cut(s) 78, 197, 393
BstSLI GKGCMC 3 cut(s) 198, 312, 508
BstV1I GCAGC 2 cut(s) 293, 309
BstV2I GAAGAC 1 cut(s) 120
BstXI CCANNNNNNTGG 1 cut(s) 190
BsuRI GGCC 3 cut(s) 83, 196, 310
BtrI CACGTC 1 cut(s) 410
BtsCI GGATG 2 cut(s) 293, 522
Cac8I GCNNGC 3 cut(s) 154, 320, 432
CfoI GCGC 1 cut(s) 228
Cfr10I RCCGGY 2 cut(s) 205, 430
Cfr13I GGNCC 6 cut(s) 24, 81, 194, 195, 308, 309
CseI GACGC 3 cut(s) 366, 423, 433
CviAII CATG 1 cut(s) 362
DdeI CTNAG 3 cut(s) 254, 500, 534
DpnI GATC 1 cut(s) 456
DpnII GATC 1 cut(s) 454
Eam1104I CTCTTC 1 cut(s) 441
EarI CTCTTC 1 cut(s) 441
Ecl136II GAGCTC 1 cut(s) 253
Eco24I GRGCYC 4 cut(s) 198, 255, 312, 419
Eco47I GGWCC 1 cut(s) 24
Eco53kI GAGCTC 1 cut(s) 253
Eco88I CYCGRG 3 cut(s) 248, 418, 510
EcoICRI GAGCTC 1 cut(s) 253
EcoNI CCTNNNNNAGG 1 cut(s) 316
EcoO109I RGGNCCY 3 cut(s) 194, 195, 309
EcoRII CCWGG 1 cut(s) 393
EcoT38I GRGCYC 4 cut(s) 198, 255, 312, 419
Esp3I CGTCTC 1 cut(s) 441
FaeI CATG 1 cut(s) 365
FaiI YATR 2 cut(s) 347, 363
FaqI GGGAC 1 cut(s) 420
FatI CATG 1 cut(s) 361
FauI CCCGC 2 cut(s) 157, 161
Fnu4HI GCNGC 6 cut(s) 276, 279, 282, 316, 323, 428
FokI GGATG 2 cut(s) 280, 529
FriOI GRGCYC 4 cut(s) 198, 255, 312, 419
Fsp4HI GCNGC 6 cut(s) 276, 279, 282, 316, 323, 428
FspBI CTAG 1 cut(s) 567
GlaI GCGC 1 cut(s) 227
GluI GCNGC 6 cut(s) 276, 279, 282, 316, 323, 428
GsaI CCCAGC 1 cut(s) 471
HaeIII GGCC 3 cut(s) 83, 196, 310
HapII CCGG 5 cut(s) 79, 102, 199, 206, 431
HgaI GACGC 3 cut(s) 366, 423, 433
HhaI GCGC 1 cut(s) 228
Hin1I GRCGYC 3 cut(s) 377, 425, 434
Hin1II CATG 1 cut(s) 365
Hin6I GCGC 1 cut(s) 226
HinP1I GCGC 1 cut(s) 226
HincII GTYRAC 1 cut(s) 7
HindII GTYRAC 1 cut(s) 7
HpaII CCGG 5 cut(s) 79, 102, 199, 206, 431
HphI GGTGA 3 cut(s) 3, 133, 382
Hpy166II GTNNAC 2 cut(s) 7, 506
Hpy188I TCNGA 1 cut(s) 28
Hpy188III TCNNGA 1 cut(s) 533
Hpy8I GTNNAC 2 cut(s) 7, 506
Hpy99I CGWCG 2 cut(s) 89, 382
HpyCH4IV ACGT 2 cut(s) 54, 409
HpyCH4V TGCA 3 cut(s) 487, 506, 559
HpyF10VI GCNNNNNNNGC 2 cut(s) 223, 281
HpyF3I CTNAG 3 cut(s) 254, 500, 534
HpySE526I ACGT 2 cut(s) 54, 409
Hsp92I GRCGYC 3 cut(s) 377, 425, 434
Hsp92II CATG 1 cut(s) 365
HspAI GCGC 1 cut(s) 226
KroI GCCGGC 1 cut(s) 430
KroNI GCCGGC 1 cut(s) 432
Kzo9I GATC 1 cut(s) 454
LmnI GCTCC 5 cut(s) 104, 222, 335, 357, 414
Lsp1109I GCAGC 2 cut(s) 293, 309
MaeI CTAG 1 cut(s) 567
MaeII ACGT 2 cut(s) 54, 409
MalI GATC 1 cut(s) 456
MboI GATC 1 cut(s) 454
MboII GAAGA 4 cut(s) 125, 458, 464, 582
MhlI GDGCHC 6 cut(s) 109, 198, 255, 312, 419, 508
MmeI TCCRAC 1 cut(s) 51
MreI CGCCGGCG 1 cut(s) 430
MroNI GCCGGC 1 cut(s) 430
MroXI GAANNNNTTC 1 cut(s) 476
MseI TTAA 1 cut(s) 598
MslI CAYNNNNRTG 1 cut(s) 188
MspI CCGG 5 cut(s) 79, 102, 199, 206, 431
MspR9I CCNGG 3 cut(s) 80, 199, 395
MvaI CCWGG 1 cut(s) 395
MwoI GCNNNNNNNGC 2 cut(s) 223, 281
NaeI GCCGGC 1 cut(s) 432
NciI CCSGG 2 cut(s) 80, 199
NdeII GATC 1 cut(s) 454
NgoMIV GCCGGC 1 cut(s) 430
NlaIII CATG 1 cut(s) 365
NlaIV GGNNCC 7 cut(s) 25, 195, 196, 197, 310, 359, 416
PaeR7I CTCGAG 1 cut(s) 248
PcsI WCGNNNNNNNCGW 3 cut(s) 60, 386, 431
PdiI GCCGGC 1 cut(s) 432
PdmI GAANNNNTTC 1 cut(s) 476
PflMI CCANNNNNTGG 1 cut(s) 367
PkrI GCNGC 6 cut(s) 277, 280, 283, 317, 324, 429
Psp124BI GAGCTC 1 cut(s) 255
Psp6I CCWGG 1 cut(s) 393
PspFI CCCAGC 1 cut(s) 467
PspGI CCWGG 1 cut(s) 393
PspN4I GGNNCC 7 cut(s) 25, 195, 196, 197, 310, 359, 416
PspOMI GGGCCC 2 cut(s) 194, 308
PspPI GGNCC 6 cut(s) 24, 81, 194, 195, 308, 309
PspXI VCTCGAGB 1 cut(s) 248
RseI CAYNNNNRTG 1 cut(s) 188
SacI GAGCTC 1 cut(s) 255
SaqAI TTAA 1 cut(s) 598
SatI GCNGC 6 cut(s) 276, 279, 282, 316, 323, 428
Sau3AI GATC 1 cut(s) 454
Sau96I GGNCC 6 cut(s) 24, 81, 194, 195, 308, 309
ScrFI CCNGG 3 cut(s) 80, 199, 395
SduI GDGCHC 6 cut(s) 109, 198, 255, 312, 419, 508
SetI ASST 8 cut(s) 57, 135, 219, 249, 255, 267, 412, 540
Sfr274I CTCGAG 1 cut(s) 248
SgrAI CRCCGGYG 1 cut(s) 430
SinI GGWCC 1 cut(s) 24
SlaI CTCGAG 1 cut(s) 248
SmiMI CAYNNNNRTG 1 cut(s) 188
SmlI CTYRAG 1 cut(s) 248
SmoI CTYRAG 1 cut(s) 248
SsiI CCGC 9 cut(s) 150, 154, 180, 273, 276, 279, 315, 428, 497
SspMI CTAG 1 cut(s) 567
SstI GAGCTC 1 cut(s) 255
StyD4I CCNGG 3 cut(s) 78, 197, 393
TaiI ACGT 2 cut(s) 57, 412
TaqI TCGA 3 cut(s) 249, 389, 457
TauI GCSGC 4 cut(s) 278, 281, 318, 430
Tru1I TTAA 1 cut(s) 598
Tru9I TTAA 1 cut(s) 598
TseI GCWGC 2 cut(s) 281, 322
Van91I CCANNNNNTGG 1 cut(s) 367
VneI GTGCAC 1 cut(s) 504
VpaK11BI GGWCC 1 cut(s) 24
XagI CCTNNNNNAGG 1 cut(s) 316
XhoI CTCGAG 1 cut(s) 248
XmnI GAANNNNTTC 1 cut(s) 476
XspI CTAG 1 cut(s) 567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.