FvH4_6g32060

IGR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0007253
Physical Location & Seq
Reverse (-)
25156290 .. 25161981
5692 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g32060.t1

Sequence Viewer

Length: 327 bp
ATGGCTTGGAGGCAAACACTGTTCCACACCAGAGCAATTTTGACTCCGCATTCAACAACTGGGCTCGCCAGATTCTCCACCAAATCAACTCCTTACTTGGAGAAAGTTGGGATACCGGAGTTTTTAAATGGAATAGGCAAAGGGGTGGAAGCTCATGTGGCCAAAATTGAATCTGAGATTGGTGACATTCAGAAGCTGCTCGTCACCCGTACTCTCAGACTGAAGAAACTTGGCATCCCTGTCAAACAAAGGAAGCTGATCTTGAAATACACCCACAAGTATAGGCTGGGACTTTGGAGGCCTCTAGCTCGCCCAATGAAATCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IGR

Protein Analysis

109

Amino Acids

12.35

Weight (kDa)

10.9

Isoelectric Point (pI)

22.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAM_Ribosomal_mS41 PF09597 31 - 97 9.7e-20 Small ribosomal subunit protein mS41 SAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 47
AcoI YGGCCR 1 cut(s) 159
AcuI CTGAAG 1 cut(s) 242
AfaI GTAC 1 cut(s) 211
AgsI TTSAA 3 cut(s) 54, 170, 265
AjuI GAANNNNNNNTTGG 2 cut(s) 162, 194
AluBI AGCT 4 cut(s) 152, 196, 256, 308
AluI AGCT 4 cut(s) 152, 196, 256, 308
AlwNI CAGNNNCTG 1 cut(s) 196
AoxI GGCC 2 cut(s) 159, 299
ApeKI GCWGC 1 cut(s) 196
AsuHPI GGTGA 2 cut(s) 194, 196
BaeI ACNNNNGTAYC 2 cut(s) 104, 137
BalI TGGCCA 1 cut(s) 161
BanII GRGCYC 1 cut(s) 66
BbvI GCAGC 1 cut(s) 183
BciVI GTATCC 1 cut(s) 105
BfaI CTAG 2 cut(s) 305, 325
BfuI GTATCC 1 cut(s) 105
BisI GCNGC 1 cut(s) 197
BlsI GCNGC 1 cut(s) 198
BmrI ACTGGG 1 cut(s) 69
BmsI GCATC 1 cut(s) 243
BmuI ACTGGG 1 cut(s) 69
BsaWI WCCGGW 1 cut(s) 115
Bse1I ACTGG 1 cut(s) 64
BseGI GGATG 1 cut(s) 234
BseMII CTCAG 2 cut(s) 165, 229
BseNI ACTGG 1 cut(s) 64
BseXI GCAGC 1 cut(s) 183
BseYI CCCAGC 1 cut(s) 286
BshFI GGCC 2 cut(s) 161, 301
BsiSI CCGG 1 cut(s) 116
BslFI GGGAC 1 cut(s) 303
BsmFI GGGAC 1 cut(s) 303
BsmI GAATGC 1 cut(s) 49
BsnI GGCC 2 cut(s) 161, 301
Bsp1286I GDGCHC 1 cut(s) 66
Bsp143I GATC 1 cut(s) 258
BspACI CCGC 1 cut(s) 47
BspANI GGCC 2 cut(s) 161, 301
BspCNI CTCAG 2 cut(s) 166, 228
BsrI ACTGG 1 cut(s) 64
BssMI GATC 1 cut(s) 258
Bst4CI ACNGT 1 cut(s) 21
BstC8I GCNNGC 2 cut(s) 66, 310
BstDEI CTNAG 2 cut(s) 174, 215
BstF5I GGATG 1 cut(s) 234
BstKTI GATC 1 cut(s) 261
BstMBI GATC 1 cut(s) 258
BstMWI GCNNNNNNNGC 1 cut(s) 158
BstV1I GCAGC 1 cut(s) 183
BsuI GTATCC 1 cut(s) 105
BsuRI GGCC 2 cut(s) 161, 301
BtsCI GGATG 1 cut(s) 234
BtsIMutI CAGTG 1 cut(s) 17
Cac8I GCNNGC 2 cut(s) 66, 310
CaiI CAGNNNCTG 1 cut(s) 196
Csp6I GTAC 1 cut(s) 210
CviAII CATG 1 cut(s) 155
CviJI RGCY 9 cut(s) 5, 64, 152, 161, 196, 256, 286, 301, 308
CviKI_1 RGCY 9 cut(s) 5, 64, 152, 161, 196, 256, 286, 301, 308
CviQI GTAC 1 cut(s) 210
DdeI CTNAG 2 cut(s) 174, 215
DpnI GATC 1 cut(s) 260
DpnII GATC 1 cut(s) 258
DraI TTTAAA 1 cut(s) 126
EaeI YGGCCR 1 cut(s) 159
Eco147I AGGCCT 1 cut(s) 301
Eco24I GRGCYC 1 cut(s) 66
Eco57I CTGAAG 1 cut(s) 242
EcoT38I GRGCYC 1 cut(s) 66
FaeI CATG 1 cut(s) 158
FaiI YATR 2 cut(s) 156, 282
FalI AAGNNNNNCTT 2 cut(s) 245, 277
FaqI GGGAC 1 cut(s) 303
FatI CATG 1 cut(s) 154
Fnu4HI GCNGC 1 cut(s) 197
FokI GGATG 1 cut(s) 221
FriOI GRGCYC 1 cut(s) 66
Fsp4HI GCNGC 1 cut(s) 197
FspBI CTAG 2 cut(s) 305, 325
GluI GCNGC 1 cut(s) 197
GsaI CCCAGC 1 cut(s) 290
HaeIII GGCC 2 cut(s) 161, 301
HapII CCGG 1 cut(s) 116
Hin1II CATG 1 cut(s) 158
HinfI GANTC 3 cut(s) 43, 72, 170
HpaII CCGG 1 cut(s) 116
HphI GGTGA 2 cut(s) 194, 196
Hpy188I TCNGA 3 cut(s) 175, 192, 218
Hpy188III TCNNGA 1 cut(s) 262
HpyCH4III ACNGT 1 cut(s) 21
HpyF10VI GCNNNNNNNGC 1 cut(s) 158
HpyF3I CTNAG 2 cut(s) 174, 215
Hsp92II CATG 1 cut(s) 158
Kzo9I GATC 1 cut(s) 258
LpnPI CCDG 6 cut(s) 43, 45, 82, 129, 252, 272
Lsp1109I GCAGC 1 cut(s) 183
LweI GCATC 1 cut(s) 243
MaeI CTAG 2 cut(s) 305, 325
MaeIII GTNAC 2 cut(s) 182, 202
MalI GATC 1 cut(s) 260
MboI GATC 1 cut(s) 258
MboII GAAGA 1 cut(s) 235
MhlI GDGCHC 1 cut(s) 66
MlsI TGGCCA 1 cut(s) 161
MluCI AATT 2 cut(s) 36, 165
MluNI TGGCCA 1 cut(s) 161
MlyI GAGTC 1 cut(s) 37
MnlI CCTC 3 cut(s) 3, 291, 312
Mox20I TGGCCA 1 cut(s) 161
MscI TGGCCA 1 cut(s) 161
MseI TTAA 1 cut(s) 125
Msp20I TGGCCA 1 cut(s) 161
MspI CCGG 1 cut(s) 116
Mva1269I GAATGC 1 cut(s) 49
MwoI GCNNNNNNNGC 1 cut(s) 158
NdeII GATC 1 cut(s) 258
NlaIII CATG 1 cut(s) 158
NmuCI GTSAC 2 cut(s) 182, 202
PceI AGGCCT 1 cut(s) 301
PctI GAATGC 1 cut(s) 49
PfeI GAWTC 2 cut(s) 72, 170
PkrI GCNGC 1 cut(s) 198
PleI GAGTC 1 cut(s) 37
PpsI GAGTC 1 cut(s) 37
PspFI CCCAGC 1 cut(s) 286
PstNI CAGNNNCTG 1 cut(s) 196
RsaI GTAC 1 cut(s) 211
RsaNI GTAC 1 cut(s) 210
SaqAI TTAA 1 cut(s) 125
SatI GCNGC 1 cut(s) 197
Sau3AI GATC 1 cut(s) 258
SchI GAGTC 1 cut(s) 37
SduI GDGCHC 1 cut(s) 66
SetI ASST 4 cut(s) 154, 198, 258, 310
SfaNI GCATC 1 cut(s) 243
Sse9I AATT 2 cut(s) 36, 165
SseBI AGGCCT 1 cut(s) 301
SsiI CCGC 1 cut(s) 47
SspMI CTAG 2 cut(s) 305, 325
StuI AGGCCT 1 cut(s) 301
TaaI ACNGT 1 cut(s) 21
TasI AATT 2 cut(s) 36, 165
TfiI GAWTC 2 cut(s) 72, 170
Tru1I TTAA 1 cut(s) 125
Tru9I TTAA 1 cut(s) 125
TscAI CASTG 1 cut(s) 24
TseFI GTSAC 2 cut(s) 182, 202
TseI GCWGC 1 cut(s) 196
Tsp45I GTSAC 2 cut(s) 182, 202
TspRI CASTG 1 cut(s) 24
XspI CTAG 2 cut(s) 305, 325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.