MD10G1090000.v1.1

IGR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0007253
Physical Location & Seq
Reverse (-)
13533082 .. 13535150
2069 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1090000.v1.1.491

Sequence Viewer

Length: 312 bp
ATGTTATTCCCCACCAGATCAATGTTGGTACCATTTTCCACAGGCGGGTTCGCCAGATTCTCCACCAAATCAAACCCTTATCTAGTGAAAGTTGGGATACCAGAGTTTTTGAATGGGATTGGCAATGGAGTGGAATCTCATGTGGCCAAGCTTGATTCTGAGATTGGCGATTTCCAAAAGCTGCTTGTCACTCGTACTCTCAGACTGAAGAAACTTGGCGTCCCTTGCAAACATAGGAAGCTGATCTTGAAATACACTCACAAGTATAGGCTGGGACTTTGGAGGCCTCGAGCTCAGCCCGTCAAAGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IGR

Protein Analysis

104

Amino Acids

11.69

Weight (kDa)

10.69

Isoelectric Point (pI)

25.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAM_Ribosomal_mS41 PF09597 32 - 92 7.6e-18 Small ribosomal subunit protein mS41 SAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 28
AccB1I GGYRCC 1 cut(s) 28
AciI CCGC 1 cut(s) 45
AcoI YGGCCR 1 cut(s) 144
AcuI CTGAAG 1 cut(s) 227
AcyI GRCGYC 1 cut(s) 219
AfaI GTAC 2 cut(s) 30, 196
AfiI CCNNNNNNNGG 1 cut(s) 45
AgsI TTSAA 2 cut(s) 112, 250
AluBI AGCT 4 cut(s) 151, 181, 241, 293
AluI AGCT 4 cut(s) 151, 181, 241, 293
Alw21I GWGCWC 1 cut(s) 295
Ama87I CYCGRG 1 cut(s) 288
AoxI GGCC 2 cut(s) 144, 284
ApeKI GCWGC 1 cut(s) 181
Asp718I GGTACC 1 cut(s) 28
AvaI CYCGRG 1 cut(s) 288
BaeI ACNNNNGTAYC 2 cut(s) 89, 122
BalI TGGCCA 1 cut(s) 146
BanI GGYRCC 1 cut(s) 28
BanII GRGCYC 1 cut(s) 295
Bbv12I GWGCWC 1 cut(s) 295
BbvI GCAGC 1 cut(s) 168
BciVI GTATCC 1 cut(s) 90
BfaI CTAG 2 cut(s) 83, 310
BfuI GTATCC 1 cut(s) 90
BisI GCNGC 1 cut(s) 182
BlpI GCTNAGC 1 cut(s) 294
BlsI GCNGC 1 cut(s) 183
BmeT110I CYCGRG 1 cut(s) 288
BmiI GGNNCC 1 cut(s) 30
Bpu1102I GCTNAGC 1 cut(s) 294
BsaHI GRCGYC 1 cut(s) 219
Bsc4I CCNNNNNNNGG 1 cut(s) 45
Bse3DI GCAATG 1 cut(s) 130
BseLI CCNNNNNNNGG 1 cut(s) 45
BseMI GCAATG 1 cut(s) 130
BseMII CTCAG 3 cut(s) 150, 214, 308
BseXI GCAGC 1 cut(s) 168
BseYI CCCAGC 1 cut(s) 271
BshFI GGCC 2 cut(s) 146, 286
BshNI GGYRCC 1 cut(s) 28
BsiHKAI GWGCWC 1 cut(s) 295
BsiHKCI CYCGRG 1 cut(s) 288
BslFI GGGAC 2 cut(s) 206, 288
BslI CCNNNNNNNGG 1 cut(s) 45
BsmFI GGGAC 2 cut(s) 206, 288
BsnI GGCC 2 cut(s) 146, 286
BsoBI CYCGRG 1 cut(s) 288
Bsp1286I GDGCHC 1 cut(s) 295
Bsp143I GATC 2 cut(s) 17, 243
Bsp1720I GCTNAGC 1 cut(s) 294
BspACI CCGC 1 cut(s) 45
BspANI GGCC 2 cut(s) 146, 286
BspCNI CTCAG 3 cut(s) 151, 213, 307
BspLI GGNNCC 1 cut(s) 30
BspT107I GGYRCC 1 cut(s) 28
BsrDI GCAATG 1 cut(s) 130
BssMI GATC 2 cut(s) 17, 243
BssNI GRCGYC 1 cut(s) 219
BstACI GRCGYC 1 cut(s) 219
BstDEI CTNAG 3 cut(s) 159, 200, 294
BstKTI GATC 2 cut(s) 20, 246
BstMBI GATC 2 cut(s) 17, 243
BstMWI GCNNNNNNNGC 1 cut(s) 225
BstV1I GCAGC 1 cut(s) 168
BsuI GTATCC 1 cut(s) 90
BsuRI GGCC 2 cut(s) 146, 286
CseI GACGC 1 cut(s) 208
Csp6I GTAC 2 cut(s) 29, 195
CviAII CATG 1 cut(s) 140
CviJI RGCY 8 cut(s) 146, 151, 181, 241, 271, 286, 293, 298
CviKI_1 RGCY 8 cut(s) 146, 151, 181, 241, 271, 286, 293, 298
CviQI GTAC 2 cut(s) 29, 195
DdeI CTNAG 3 cut(s) 159, 200, 294
DpnI GATC 2 cut(s) 19, 245
DpnII GATC 2 cut(s) 17, 243
EaeI YGGCCR 1 cut(s) 144
Ecl136II GAGCTC 1 cut(s) 293
Eco147I AGGCCT 1 cut(s) 286
Eco24I GRGCYC 1 cut(s) 295
Eco53kI GAGCTC 1 cut(s) 293
Eco57I CTGAAG 1 cut(s) 227
Eco88I CYCGRG 1 cut(s) 288
EcoICRI GAGCTC 1 cut(s) 293
EcoT38I GRGCYC 1 cut(s) 295
FaeI CATG 1 cut(s) 143
FaiI YATR 3 cut(s) 141, 234, 267
FalI AAGNNNNNCTT 2 cut(s) 230, 262
FaqI GGGAC 2 cut(s) 206, 288
FatI CATG 1 cut(s) 139
FauI CCCGC 1 cut(s) 38
Fnu4HI GCNGC 1 cut(s) 182
FriOI GRGCYC 1 cut(s) 295
Fsp4HI GCNGC 1 cut(s) 182
FspBI CTAG 2 cut(s) 83, 310
GluI GCNGC 1 cut(s) 182
GsaI CCCAGC 1 cut(s) 275
HaeIII GGCC 2 cut(s) 146, 286
HgaI GACGC 1 cut(s) 208
Hin1I GRCGYC 1 cut(s) 219
Hin1II CATG 1 cut(s) 143
HindIII AAGCTT 1 cut(s) 149
HinfI GANTC 3 cut(s) 57, 134, 155
Hpy188I TCNGA 2 cut(s) 160, 203
Hpy188III TCNNGA 1 cut(s) 247
HpyCH4V TGCA 1 cut(s) 228
HpyF10VI GCNNNNNNNGC 1 cut(s) 225
HpyF3I CTNAG 3 cut(s) 159, 200, 294
Hsp92I GRCGYC 1 cut(s) 219
Hsp92II CATG 1 cut(s) 143
KpnI GGTACC 1 cut(s) 32
Kzo9I GATC 2 cut(s) 17, 243
LpnPI CCDG 5 cut(s) 27, 28, 67, 114, 257
Lsp1109I GCAGC 1 cut(s) 168
MaeI CTAG 2 cut(s) 83, 310
MaeIII GTNAC 1 cut(s) 187
MalI GATC 2 cut(s) 19, 245
MboI GATC 2 cut(s) 17, 243
MboII GAAGA 1 cut(s) 220
MhlI GDGCHC 1 cut(s) 295
MlsI TGGCCA 1 cut(s) 146
MluNI TGGCCA 1 cut(s) 146
MnlI CCTC 2 cut(s) 276, 297
Mox20I TGGCCA 1 cut(s) 146
MscI TGGCCA 1 cut(s) 146
Msp20I TGGCCA 1 cut(s) 146
MwoI GCNNNNNNNGC 1 cut(s) 225
NdeII GATC 2 cut(s) 17, 243
NlaIII CATG 1 cut(s) 143
NlaIV GGNNCC 1 cut(s) 30
NmuCI GTSAC 1 cut(s) 187
PaeR7I CTCGAG 1 cut(s) 288
PceI AGGCCT 1 cut(s) 286
PfeI GAWTC 3 cut(s) 57, 134, 155
PkrI GCNGC 1 cut(s) 183
Psp124BI GAGCTC 1 cut(s) 295
PspFI CCCAGC 1 cut(s) 271
PspN4I GGNNCC 1 cut(s) 30
PspXI VCTCGAGB 1 cut(s) 288
RsaI GTAC 2 cut(s) 30, 196
RsaNI GTAC 2 cut(s) 29, 195
SacI GAGCTC 1 cut(s) 295
SatI GCNGC 1 cut(s) 182
Sau3AI GATC 2 cut(s) 17, 243
SduI GDGCHC 1 cut(s) 295
SetI ASST 4 cut(s) 153, 183, 243, 295
Sfr274I CTCGAG 1 cut(s) 288
SlaI CTCGAG 1 cut(s) 288
SmlI CTYRAG 1 cut(s) 288
SmoI CTYRAG 1 cut(s) 288
SseBI AGGCCT 1 cut(s) 286
SsiI CCGC 1 cut(s) 45
SspMI CTAG 2 cut(s) 83, 310
SstI GAGCTC 1 cut(s) 295
StuI AGGCCT 1 cut(s) 286
TaqI TCGA 1 cut(s) 289
TfiI GAWTC 3 cut(s) 57, 134, 155
TseFI GTSAC 1 cut(s) 187
TseI GCWGC 1 cut(s) 181
Tsp45I GTSAC 1 cut(s) 187
XcmI CCANNNNNNNNNTGG 1 cut(s) 22
XhoI CTCGAG 1 cut(s) 288
XspI CTAG 2 cut(s) 83, 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.