pycom10g07350

IGR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0007253
Physical Location & Seq
Reverse (-)
8681272 .. 8683050
1779 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g07350.1

Sequence Viewer

Length: 264 bp
ATGGAGGCAAATGTTATTCCCCACCAGATCAATGTTGGTACCATTTTCCACAGCCGGGTTCGCCAGTCAAACCCTTATCTAGTGAAAGTTGGGATACCAGAGTTTTTGAATGGGATTGGCAATGGAGTGGAATCCCATGTGGCCAAGCTTGATTCTGAGATTGGCGATTTCCAAAAGCTGCTTGTCACTCGTACTCTCAGACTGAAGAAACTTGGCGTCCCTTGCAAACATGAAGCTGATCTTGAAATACACTCACAAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IGR

Protein Analysis

88

Amino Acids

9.68

Weight (kDa)

7.97

Isoelectric Point (pI)

20.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAM_Ribosomal_mS41 PF09597 31 - 77 7.8e-10 Small ribosomal subunit protein mS41 SAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 38
AccB1I GGYRCC 1 cut(s) 38
AcoI YGGCCR 1 cut(s) 141
AcuI CTGAAG 1 cut(s) 224
AcyI GRCGYC 1 cut(s) 216
AfaI GTAC 2 cut(s) 40, 193
AfiI CCNNNNNNNGG 1 cut(s) 55
AgsI TTSAA 2 cut(s) 109, 245
AluBI AGCT 3 cut(s) 148, 178, 236
AluI AGCT 3 cut(s) 148, 178, 236
AoxI GGCC 1 cut(s) 141
ApeKI GCWGC 1 cut(s) 178
Asp718I GGTACC 1 cut(s) 38
AsuC2I CCSGG 1 cut(s) 56
BaeI ACNNNNGTAYC 2 cut(s) 86, 119
BalI TGGCCA 1 cut(s) 143
BanI GGYRCC 1 cut(s) 38
BbvI GCAGC 1 cut(s) 165
BciVI GTATCC 1 cut(s) 87
BcnI CCSGG 1 cut(s) 56
BfaI CTAG 1 cut(s) 80
BfuI GTATCC 1 cut(s) 87
BisI GCNGC 1 cut(s) 179
BlsI GCNGC 1 cut(s) 180
Bme1390I CCNGG 1 cut(s) 56
BmiI GGNNCC 1 cut(s) 40
BmrFI CCNGG 1 cut(s) 56
BpuMI CCSGG 1 cut(s) 56
BsaHI GRCGYC 1 cut(s) 216
Bsc4I CCNNNNNNNGG 1 cut(s) 55
Bse1I ACTGG 1 cut(s) 64
Bse3DI GCAATG 1 cut(s) 127
BseLI CCNNNNNNNGG 1 cut(s) 55
BseMI GCAATG 1 cut(s) 127
BseMII CTCAG 2 cut(s) 147, 211
BseNI ACTGG 1 cut(s) 64
BseXI GCAGC 1 cut(s) 165
BshFI GGCC 1 cut(s) 143
BshNI GGYRCC 1 cut(s) 38
BsiSI CCGG 1 cut(s) 55
BslFI GGGAC 1 cut(s) 203
BslI CCNNNNNNNGG 1 cut(s) 55
BsmFI GGGAC 1 cut(s) 203
BsnI GGCC 1 cut(s) 143
Bsp143I GATC 2 cut(s) 27, 238
BspANI GGCC 1 cut(s) 143
BspCNI CTCAG 2 cut(s) 148, 210
BspLI GGNNCC 1 cut(s) 40
BspT107I GGYRCC 1 cut(s) 38
BsrDI GCAATG 1 cut(s) 127
BsrI ACTGG 1 cut(s) 64
BssMI GATC 2 cut(s) 27, 238
BssNI GRCGYC 1 cut(s) 216
BstACI GRCGYC 1 cut(s) 216
BstDEI CTNAG 2 cut(s) 156, 197
BstKTI GATC 2 cut(s) 30, 241
BstMBI GATC 2 cut(s) 27, 238
BstMWI GCNNNNNNNGC 2 cut(s) 60, 222
BstSCI CCNGG 1 cut(s) 54
BstV1I GCAGC 1 cut(s) 165
BsuI GTATCC 1 cut(s) 87
BsuRI GGCC 1 cut(s) 143
CseI GACGC 1 cut(s) 205
Csp6I GTAC 2 cut(s) 39, 192
CviAII CATG 2 cut(s) 137, 230
CviJI RGCY 5 cut(s) 54, 143, 148, 178, 236
CviKI_1 RGCY 5 cut(s) 54, 143, 148, 178, 236
CviQI GTAC 2 cut(s) 39, 192
DdeI CTNAG 2 cut(s) 156, 197
DpnI GATC 2 cut(s) 29, 240
DpnII GATC 2 cut(s) 27, 238
EaeI YGGCCR 1 cut(s) 141
Eco57I CTGAAG 1 cut(s) 224
FaeI CATG 2 cut(s) 140, 233
FaiI YATR 3 cut(s) 138, 231, 262
FalI AAGNNNNNCTT 2 cut(s) 225, 257
FaqI GGGAC 1 cut(s) 203
FatI CATG 2 cut(s) 136, 229
Fnu4HI GCNGC 1 cut(s) 179
Fsp4HI GCNGC 1 cut(s) 179
FspBI CTAG 1 cut(s) 80
GluI GCNGC 1 cut(s) 179
HaeIII GGCC 1 cut(s) 143
HapII CCGG 1 cut(s) 55
HgaI GACGC 1 cut(s) 205
Hin1I GRCGYC 1 cut(s) 216
Hin1II CATG 2 cut(s) 140, 233
HindIII AAGCTT 1 cut(s) 146
HinfI GANTC 2 cut(s) 131, 152
HpaII CCGG 1 cut(s) 55
Hpy188I TCNGA 2 cut(s) 157, 200
Hpy188III TCNNGA 1 cut(s) 242
HpyCH4V TGCA 1 cut(s) 225
HpyF10VI GCNNNNNNNGC 2 cut(s) 60, 222
HpyF3I CTNAG 2 cut(s) 156, 197
Hsp92I GRCGYC 1 cut(s) 216
Hsp92II CATG 2 cut(s) 140, 233
KpnI GGTACC 1 cut(s) 42
Kzo9I GATC 2 cut(s) 27, 238
LpnPI CCDG 4 cut(s) 38, 68, 77, 111
Lsp1109I GCAGC 1 cut(s) 165
MaeI CTAG 1 cut(s) 80
MaeIII GTNAC 1 cut(s) 184
MalI GATC 2 cut(s) 29, 240
MboI GATC 2 cut(s) 27, 238
MboII GAAGA 1 cut(s) 217
MlsI TGGCCA 1 cut(s) 143
MluNI TGGCCA 1 cut(s) 143
Mox20I TGGCCA 1 cut(s) 143
MscI TGGCCA 1 cut(s) 143
Msp20I TGGCCA 1 cut(s) 143
MspI CCGG 1 cut(s) 55
MspR9I CCNGG 1 cut(s) 56
MwoI GCNNNNNNNGC 2 cut(s) 60, 222
NciI CCSGG 1 cut(s) 56
NdeII GATC 2 cut(s) 27, 238
NlaIII CATG 2 cut(s) 140, 233
NlaIV GGNNCC 1 cut(s) 40
NmuCI GTSAC 1 cut(s) 184
PfeI GAWTC 2 cut(s) 131, 152
PkrI GCNGC 1 cut(s) 180
PspN4I GGNNCC 1 cut(s) 40
RsaI GTAC 2 cut(s) 40, 193
RsaNI GTAC 2 cut(s) 39, 192
SatI GCNGC 1 cut(s) 179
Sau3AI GATC 2 cut(s) 27, 238
ScrFI CCNGG 1 cut(s) 56
SetI ASST 3 cut(s) 150, 180, 238
SspMI CTAG 1 cut(s) 80
StyD4I CCNGG 1 cut(s) 54
TfiI GAWTC 2 cut(s) 131, 152
TseFI GTSAC 1 cut(s) 184
TseI GCWGC 1 cut(s) 178
Tsp45I GTSAC 1 cut(s) 184
TspDTI ATGAA 1 cut(s) 246
XcmI CCANNNNNNNNNTGG 1 cut(s) 32
XspI CTAG 1 cut(s) 80
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.