MD07G1055000.v1.1

IGR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0007253
Physical Location & Seq
Reverse (-)
5082725 .. 5085178
2454 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1055000.v1.1.491

Sequence Viewer

Length: 282 bp
ATGTTTTCGACACCGGGGTTGGCCAGATTCTCCACCAAATCAAACCCATACCTAGTGAAAGTTGGGATACCAGAGTTTTTGAATGGCATTGGCAATGGAGTGGAATCCCATGTAACCAAGCTTGAGTCTGAGATCAGAGACTTCCAAAAGTTGCTTGTCATTCGTACTCTCAGGCTGAAGAAACTTGGCGTCCCTTGCAAGCATAGGAAGCTCATCTTGAAACACACTCACAAGTATAGGCTAGGACTCTGGAGGCCTCGAGCTCAGCCCATCAAACCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IGR

Protein Analysis

94

Amino Acids

10.7

Weight (kDa)

10.87

Isoelectric Point (pI)

13.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAM_Ribosomal_mS41 PF09597 22 - 82 6.3e-18 Small ribosomal subunit protein mS41 SAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 21
AcuI CTGAAG 1 cut(s) 197
AcyI GRCGYC 1 cut(s) 189
AfaI GTAC 1 cut(s) 166
AgsI TTSAA 2 cut(s) 82, 220
AluBI AGCT 3 cut(s) 121, 211, 263
AluI AGCT 3 cut(s) 121, 211, 263
Alw21I GWGCWC 1 cut(s) 265
Alw26I GTCTC 1 cut(s) 132
Ama87I CYCGRG 1 cut(s) 258
AoxI GGCC 2 cut(s) 21, 254
AsuC2I CCSGG 1 cut(s) 15
AvaI CYCGRG 1 cut(s) 258
BaeI ACNNNNGTAYC 2 cut(s) 59, 92
BalI TGGCCA 1 cut(s) 23
BanII GRGCYC 1 cut(s) 265
Bbv12I GWGCWC 1 cut(s) 265
BccI CCATC 1 cut(s) 278
BciVI GTATCC 1 cut(s) 60
BcnI CCSGG 1 cut(s) 15
BcoDI GTCTC 1 cut(s) 132
BfaI CTAG 3 cut(s) 53, 242, 280
BfuI GTATCC 1 cut(s) 60
BlpI GCTNAGC 1 cut(s) 264
Bme1390I CCNGG 1 cut(s) 15
BmeT110I CYCGRG 1 cut(s) 258
BmrFI CCNGG 1 cut(s) 15
BpmI CTGGAG 1 cut(s) 271
Bpu1102I GCTNAGC 1 cut(s) 264
BpuEI CTTGAG 1 cut(s) 143
BpuMI CCSGG 1 cut(s) 15
BsaHI GRCGYC 1 cut(s) 189
BsaJI CCNNGG 1 cut(s) 14
Bse3DI GCAATG 1 cut(s) 100
BseDI CCNNGG 1 cut(s) 14
BseMI GCAATG 1 cut(s) 100
BseMII CTCAG 3 cut(s) 120, 184, 278
BshFI GGCC 2 cut(s) 23, 256
BsiHKAI GWGCWC 1 cut(s) 265
BsiHKCI CYCGRG 1 cut(s) 258
BsiSI CCGG 1 cut(s) 14
BslFI GGGAC 1 cut(s) 176
BsmAI GTCTC 1 cut(s) 132
BsmFI GGGAC 1 cut(s) 176
BsnI GGCC 2 cut(s) 23, 256
BsoBI CYCGRG 1 cut(s) 258
Bsp1286I GDGCHC 1 cut(s) 265
Bsp143I GATC 1 cut(s) 132
Bsp1720I GCTNAGC 1 cut(s) 264
BspANI GGCC 2 cut(s) 23, 256
BspCNI CTCAG 3 cut(s) 121, 183, 277
BsrDI GCAATG 1 cut(s) 100
BssECI CCNNGG 1 cut(s) 14
BssMI GATC 1 cut(s) 132
BssNI GRCGYC 1 cut(s) 189
BstACI GRCGYC 1 cut(s) 189
BstC8I GCNNGC 1 cut(s) 200
BstDEI CTNAG 3 cut(s) 129, 170, 264
BstKTI GATC 1 cut(s) 135
BstMAI GTCTC 1 cut(s) 132
BstMBI GATC 1 cut(s) 132
BstMWI GCNNNNNNNGC 2 cut(s) 195, 208
BstSCI CCNGG 1 cut(s) 13
BsuI GTATCC 1 cut(s) 60
BsuRI GGCC 2 cut(s) 23, 256
Cac8I GCNNGC 1 cut(s) 200
CseI GACGC 1 cut(s) 178
Csp6I GTAC 1 cut(s) 165
CviAII CATG 1 cut(s) 110
CviJI RGCY 8 cut(s) 23, 121, 175, 211, 241, 256, 263, 268
CviKI_1 RGCY 8 cut(s) 23, 121, 175, 211, 241, 256, 263, 268
CviQI GTAC 1 cut(s) 165
DdeI CTNAG 3 cut(s) 129, 170, 264
DpnI GATC 1 cut(s) 134
DpnII GATC 1 cut(s) 132
EaeI YGGCCR 1 cut(s) 21
Ecl136II GAGCTC 1 cut(s) 263
Eco147I AGGCCT 1 cut(s) 256
Eco24I GRGCYC 1 cut(s) 265
Eco53kI GAGCTC 1 cut(s) 263
Eco57I CTGAAG 1 cut(s) 197
Eco88I CYCGRG 1 cut(s) 258
EcoICRI GAGCTC 1 cut(s) 263
EcoT38I GRGCYC 1 cut(s) 265
FaeI CATG 1 cut(s) 113
FaiI YATR 4 cut(s) 49, 111, 204, 237
FalI AAGNNNNNCTT 2 cut(s) 200, 232
FaqI GGGAC 1 cut(s) 176
FatI CATG 1 cut(s) 109
FriOI GRGCYC 1 cut(s) 265
FspBI CTAG 3 cut(s) 53, 242, 280
GsuI CTGGAG 1 cut(s) 271
HaeIII GGCC 2 cut(s) 23, 256
HapII CCGG 1 cut(s) 14
HgaI GACGC 1 cut(s) 178
Hin1I GRCGYC 1 cut(s) 189
Hin1II CATG 1 cut(s) 113
HindIII AAGCTT 1 cut(s) 119
HinfI GANTC 4 cut(s) 27, 104, 125, 246
HpaII CCGG 1 cut(s) 14
Hpy188I TCNGA 2 cut(s) 130, 137
Hpy188III TCNNGA 2 cut(s) 217, 250
HpyCH4V TGCA 1 cut(s) 198
HpyF10VI GCNNNNNNNGC 2 cut(s) 195, 208
HpyF3I CTNAG 3 cut(s) 129, 170, 264
Hsp92I GRCGYC 1 cut(s) 189
Hsp92II CATG 1 cut(s) 113
Kzo9I GATC 1 cut(s) 132
LpnPI CCDG 5 cut(s) 27, 37, 84, 157, 235
MaeI CTAG 3 cut(s) 53, 242, 280
MaeIII GTNAC 1 cut(s) 112
MalI GATC 1 cut(s) 134
MboI GATC 1 cut(s) 132
MboII GAAGA 1 cut(s) 190
MhlI GDGCHC 1 cut(s) 265
MlsI TGGCCA 1 cut(s) 23
MluNI TGGCCA 1 cut(s) 23
MlyI GAGTC 2 cut(s) 134, 240
MnlI CCTC 2 cut(s) 246, 267
Mox20I TGGCCA 1 cut(s) 23
MscI TGGCCA 1 cut(s) 23
Msp20I TGGCCA 1 cut(s) 23
MspI CCGG 1 cut(s) 14
MspR9I CCNGG 1 cut(s) 15
MwoI GCNNNNNNNGC 2 cut(s) 195, 208
NciI CCSGG 1 cut(s) 15
NdeII GATC 1 cut(s) 132
NlaIII CATG 1 cut(s) 113
PaeR7I CTCGAG 1 cut(s) 258
PceI AGGCCT 1 cut(s) 256
PfeI GAWTC 2 cut(s) 27, 104
PleI GAGTC 2 cut(s) 133, 240
PpsI GAGTC 2 cut(s) 133, 240
Psp124BI GAGCTC 1 cut(s) 265
PspXI VCTCGAGB 1 cut(s) 258
RsaI GTAC 1 cut(s) 166
RsaNI GTAC 1 cut(s) 165
SacI GAGCTC 1 cut(s) 265
Sau3AI GATC 1 cut(s) 132
SchI GAGTC 2 cut(s) 134, 240
ScrFI CCNGG 1 cut(s) 15
SduI GDGCHC 1 cut(s) 265
SetI ASST 4 cut(s) 54, 123, 213, 265
Sfr274I CTCGAG 1 cut(s) 258
SlaI CTCGAG 1 cut(s) 258
SmlI CTYRAG 2 cut(s) 122, 258
SmoI CTYRAG 2 cut(s) 122, 258
SseBI AGGCCT 1 cut(s) 256
SspMI CTAG 3 cut(s) 53, 242, 280
SstI GAGCTC 1 cut(s) 265
StuI AGGCCT 1 cut(s) 256
StyD4I CCNGG 1 cut(s) 13
TaqI TCGA 2 cut(s) 8, 259
TfiI GAWTC 2 cut(s) 27, 104
XhoI CTCGAG 1 cut(s) 258
XspI CTAG 3 cut(s) 53, 242, 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.