Rorug02G0359600

IGR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0007253
Physical Location & Seq
Forward (+)
45513451 .. 45527231
13781 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0359600.1

Sequence Viewer

Length: 2958 bp
ATGGCTGAAGCCCTTATTTCCGTTGTCCTAGAACAACTGGCTTCAATCACCCGTCAACAGGTACAGCAACAAGTGAAATTGGTAGTTGATGTTAAGAAAGAAGTTGCAAATCTCAGAGACAATTTCCGAGCTATAAAAGCCGTGCTCAAAGATGCAGAGGAGAGGCAAGTGAAGGAGGATTCCGTCAAACTATGGCTGGATAATATAAGGGACGTGTCCAACGAGATGGAGGACGTGTTGGATGATTGGAACACTGAAATTCTGAAGCTACAAATTGAGAAACAAGAGAAAGAAGATGGAAATCCTCTTGATACGACTAAGAAGAAGGTATGTCTATGCATTCCTACTGCTTTCTTTTGTTCTGGCCAAGTTACTCGATTTATTTCTCGTCGCGACATTGCTCAAAAAATTAAAGATCTCAATGAAAGGTTAGCAAAAATTGCTAATATGAGGCAAAATTTTAACTTTCAGTATCCAAAAAGTGGTACTGAACTTATTGAGCGACAAAAGACTACTTCTTTTGTCGATCAAACATTTGGTCGGGTAGATGAAAAAGAATTATTAGTGGACATGTTGTTGAGTGAAAGTAATCCTGAAGGGCAAAGCCCCATTGTCATCCCTATTGTAGGGATGGGTGGAATCGGTAAAACAACCCTTTCTCAACTAGCATATAATGATAAAAGAGTCCAGGAAAATTTCAAAGATAGAATATGGGTTTGTGTCTCAGATCCATTTGATGAGCTCAACATAGCCAAAACCATCCTTGCACGCCTTGGTGGAAATGTCAATTCCATTTCGAGTGACCTAGATGCTTTCTTCGAATGTCTGCAGAAGTCTATTGAGGGGAAAAAGTTTCTCCTCGTCTTAGATGATGTGTGGAACCAAGACTATATGAAGTGGGCAAACTTGAAGCTACATTTACAGAAAGGTGTTGTAGGTAGTAAAATAATTGTCACTACACGAAAAGAAGAAGTAGCCACGATGATGGGAGCACCTGATAACACGATCAATTTGAAGGTGTTGAGTGACGAAAATTGTTGGTCATTGTTCGCTGGAATTGCCTTTACCAATAGAAACCAAGATGAGTGTCAAATTTTGGAACCTATTGGTAGGGAAATTGTAAAGAAGTGCAAAGGGTTGCCTCTTGTTGCCAAGACTTTAGGTGGTCTCATGCGTTGTAAGAAAGCAAAGAAAGAATGGCAAGATGTTTTGAGTAGTGAGATATGGGAGTTAGACACAATGGAGCAACAAGTTTTCCAACCGCTATTACTAAGTTATTATGATTTAACTCCAATGATCCAACGTTGTCTATTATATTGTGTCACTTTCCCAAAAGACCTTTTAATTAAGAAAAAAGTTTTGATTGAGTTGTGGATGTCGCAAGGTTTTCTTGGTTCAGTTGGGAACAAAAAAAAGACAATAATAGACGTTGGAGAATCATATTTTGATAACTTGGTAATGCGATCTTTCTTCCAGAATTTCAAACAAGATAAATTTGGAGATATTAAACAGTGCCAAATGCATAATATTGTGCACGACTTTCTCCAATATTTAACCAAGAATGAATGCTTAATCGTTGAGGTCAAAGGTGGTGAGAAGAGAATAGAATTACCAGCAGACAATAAGCTTGGTCATTTGACCATAACGTTTGCACCCAAGGGACCTTTTCCCGTTTCTTTAGACCATTGCCAACGTCTGCGGACCATTACAACATTTGATTGTAAAATTACAAGTCTCAGCCGAGAGTTGATTTTACAATTAAAATGCGTGAGGACGTTGAATTTGAGTGACAATTTGATCGAAGAAGTTCCGGACGAGGTTGGCGTGTTGGTGCACTTGAGGTATTTGGATTTATCGGATAATTGGAGGCTGAAGAAATTACCTGATAGTTTGTGTAATTTAGTCAATTTGCAAACCTTGCGAGTTGATGAGTGCCATGGCCTTAAAAAGTTACCCGAGGCCATGGGAAAGTTGACCAGCTTGCAGCATCTTCATGCTAAGATTGGTAGGTGGCCACCGCTGAAGTTGCCCAAATCAATTGCGAGGTTGAAAAGTCTACGGACGCTAGATCTGGATGTTATTATTGGAGAAGGTGAAGGTGAAGGTGAAGGTGAAGGTAGTAGAGATGAAGAAGGATTCAGATTAAGTGATTTGAGAGACATGGACGAGCTTCAGGGGAAGCTTAGGATTGAATTGGTGGGCAAATTGAAAGATAGTGCTGCGAGTGATGCGGAGAAAGCCAAATTGGTGAACAAGAAACACCTTCTTCATTTGGGACTAGGGTTTCCCTGCGAGAGCGAGAGTGAGGGCAACCAGAGTACCATCGAGGAAGCAGTATTGAATGCCTTACAGCCAAATTCAAATCTTGAATCCTTATGGATCGAAGGTTACGATGGCAGCACCCTGTACCCCAATTGGACCTCCTCTTTAATAAACTTGAAAAGCCTGACCTTCTCTCGGTGCAATGTTTGTAACCATGTGCCTCTTTCGGTTTTGGGCTACTTGGGGTCCCTTGAGACACTCACATTTCAACACATGGAAAAAGTGAAAAAGGTTGGATTTGAGTACTCACTTGATCATCAAGTTATATTGTTCCCCAATCTGAAACAACTCGAGTTCTCTTTCATGGGTGAGTGGGAAGAATGCGATGATGATGAGAGTGATGCTACTTCATCGTCACCAACTTGTTTCATGCCACGCCTTTCTACTTTATCCATCGAGTACTGCTTTAAGCTGAAGACACTGCCAGACTTCCTTCCAAGGAAGACACCGCTGCTTCAGAATTTGATCATCAACGGTTGTTTCATTCTCTCCGAAAGTTGCAAAGACAGGCAAGGCCCGGAATGGTCCAAGATTTCTCACATCCCAAACATCGCAATTGATGGCAAAATTGTACAAAAAGATGGAGTTTGGATCATACAAGAGGAAGAAAGTGATGAAGAAATTGATTCAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IGR

Protein Analysis

985

Amino Acids

111.98

Weight (kDa)

5.54

Isoelectric Point (pI)

42.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 6 - 95 8e-22 Rx N-terminal domain
NB-ARC PF00931 186 - 358 2e-37 NB-ARC domain
WHD_DRP PF23559 443 - 514 8.4e-23 Disease resistance protein Winged helix domain
LRR_14 PF23598 566 - 907 2.3e-28 Leucine-rich repeat region
LRR_8 PF13855 590 - 645 9.3e-08 Leucine rich repeat
LRR_R13L1-DRL21 PF25019 715 - 846 6.4e-28 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 482
AccI GTMKAC 1 cut(s) 2056
AccII CGCG 1 cut(s) 393
AccIII TCCGGA 1 cut(s) 1810
AciI CCGC 5 cut(s) 1262, 1699, 2018, 2231, 2771
AclI AACGTT 2 cut(s) 1303, 1646
AclWI GGATC 4 cut(s) 722, 1291, 2387, 2921
AcoI YGGCCR 2 cut(s) 364, 2012
AcsI RAATTY 9 cut(s) 258, 457, 694, 1092, 1477, 1493, 1780, 2356, 2782
AcuI CTGAAG 8 cut(s) 27, 284, 615, 1892, 2042, 2156, 2756, 2762
AfaI GTAC 7 cut(s) 63, 487, 2320, 2408, 2567, 2723, 2895
AfiI CCNNNNNNNGG 4 cut(s) 58, 482, 626, 2457
AflIII ACRYGT 3 cut(s) 213, 234, 570
AjiI CACGTC 2 cut(s) 214, 235
AjnI CCWGG 1 cut(s) 687
AjuI GAANNNNNNNTTGG 2 cut(s) 2228, 2260
AluBI AGCT 9 cut(s) 131, 268, 742, 913, 1627, 1980, 2170, 2182, 2734
AluI AGCT 9 cut(s) 131, 268, 742, 913, 1627, 1980, 2170, 2182, 2734
Alw21I GWGCWC 5 cut(s) 147, 744, 994, 1536, 1836
Alw26I GTCTC 6 cut(s) 111, 727, 1172, 1739, 2151, 2510
Alw44I GTGCAC 2 cut(s) 1532, 1832
AlwI GGATC 4 cut(s) 722, 1291, 2387, 2921
Ama87I CYCGRG 2 cut(s) 1955, 2612
Aor13HI TCCGGA 1 cut(s) 1810
AoxI GGCC 5 cut(s) 364, 1939, 1959, 2012, 2836
ApaLI GTGCAC 2 cut(s) 1532, 1832
ApeKI GCWGC 4 cut(s) 1984, 2219, 2397, 2773
ApoI RAATTY 9 cut(s) 258, 457, 694, 1092, 1477, 1493, 1780, 2356, 2782
ArsI GACNNNNNNTTYG 4 cut(s) 523, 555, 1765, 1797
Asp700I GAANNNNTTC 1 cut(s) 1806
AspS9I GGNCC 6 cut(s) 1661, 1701, 2418, 2508, 2837, 2847
AsuC2I CCSGG 1 cut(s) 2840
AsuHPI GGTGA 9 cut(s) 40, 1604, 2105, 2111, 2117, 2123, 2260, 2642, 2670
AsuII TTCGAA 1 cut(s) 819
AvaI CYCGRG 2 cut(s) 1955, 2612
AvaII GGWCC 5 cut(s) 1661, 1701, 2418, 2508, 2847
BaeGI GKGCMC 2 cut(s) 1536, 1836
BaeI ACNNNNGTAYC 2 cut(s) 2302, 2335
BalI TGGCCA 2 cut(s) 366, 2014
BanII GRGCYC 1 cut(s) 744
BbsI GAAGAC 2 cut(s) 2744, 2771
Bbv12I GWGCWC 5 cut(s) 147, 744, 994, 1536, 1836
BbvI GCAGC 4 cut(s) 1996, 2206, 2409, 2760
BceAI ACGGC 1 cut(s) 125
BciT130I CCWGG 1 cut(s) 689
BciVI GTATCC 1 cut(s) 483
BclI TGATCA 2 cut(s) 2575, 2787
BcnI CCSGG 1 cut(s) 2840
BcoDI GTCTC 6 cut(s) 111, 727, 1172, 1739, 2151, 2510
BfaI CTAG 5 cut(s) 29, 665, 806, 2066, 2279
BfmI CTRYAG 1 cut(s) 827
BfuI GTATCC 1 cut(s) 483
BglII AGATCT 2 cut(s) 415, 2068
BisI GCNGC 4 cut(s) 1985, 2220, 2398, 2774
BlsI GCNGC 4 cut(s) 1986, 2221, 2399, 2775
BmcAI AGTACT 2 cut(s) 2567, 2723
Bme1390I CCNGG 2 cut(s) 689, 2840
Bme18I GGWCC 5 cut(s) 1661, 1701, 2418, 2508, 2847
BmeT110I CYCGRG 2 cut(s) 1955, 2612
BmgBI CACGTC 2 cut(s) 214, 235
BmgT120I GGNCC 6 cut(s) 1661, 1701, 2418, 2508, 2837, 2847
BmiI GGNNCC 5 cut(s) 881, 1101, 1662, 2509, 2510
BmrFI CCNGG 2 cut(s) 689, 2840
BmsI GCATC 5 cut(s) 142, 799, 1996, 2218, 2653
BpiI GAAGAC 2 cut(s) 2744, 2771
Bpu10I CCTNAGC 1 cut(s) 2183
Bpu14I TTCGAA 1 cut(s) 819
BpuEI CTTGAG 2 cut(s) 1858, 2534
BpuMI CCSGG 1 cut(s) 2840
BsaBI GATNNNNATC 1 cut(s) 300
BsaI GGTCTC 1 cut(s) 1172
BsaJI CCNNGG 6 cut(s) 772, 1656, 1936, 1956, 1962, 2759
BsaWI WCCGGW 1 cut(s) 1810
BsaXI ACNNNNNCTCC 2 cut(s) 221, 251
Bsc4I CCNNNNNNNGG 4 cut(s) 58, 482, 626, 2457
Bse1I ACTGG 1 cut(s) 42
Bse3DI GCAATG 3 cut(s) 396, 1684, 2470
Bse8I GATNNNNATC 1 cut(s) 300
BseAI TCCGGA 1 cut(s) 1810
BseBI CCWGG 1 cut(s) 689
BseDI CCNNGG 6 cut(s) 772, 1656, 1936, 1956, 1962, 2759
BseGI GGATG 7 cut(s) 247, 615, 636, 759, 1380, 2080, 2862
BseJI GATNNNNATC 1 cut(s) 300
BseLI CCNNNNNNNGG 4 cut(s) 58, 482, 626, 2457
BseMI GCAATG 3 cut(s) 396, 1684, 2470
BseMII CTCAG 3 cut(s) 127, 738, 1750
BseNI ACTGG 1 cut(s) 42
BseRI GAGGAG 3 cut(s) 173, 848, 2413
BseSI GKGCMC 2 cut(s) 1536, 1836
BseXI GCAGC 4 cut(s) 1996, 2206, 2409, 2760
Bsh1236I CGCG 1 cut(s) 393
BshFI GGCC 5 cut(s) 366, 1941, 1961, 2014, 2838
BsiHKAI GWGCWC 5 cut(s) 147, 744, 994, 1536, 1836
BsiHKCI CYCGRG 2 cut(s) 1955, 2612
BsiSI CCGG 2 cut(s) 1811, 2840
BslFI GGGAC 4 cut(s) 224, 1674, 2289, 2494
BslI CCNNNNNNNGG 4 cut(s) 58, 482, 626, 2457
BsmAI GTCTC 6 cut(s) 111, 727, 1172, 1739, 2151, 2510
BsmFI GGGAC 4 cut(s) 224, 1674, 2289, 2494
BsmI GAATGC 4 cut(s) 339, 1571, 2347, 2648
BsnI GGCC 5 cut(s) 366, 1941, 1961, 2014, 2838
Bso31I GGTCTC 1 cut(s) 1172
BsoBI CYCGRG 2 cut(s) 1955, 2612
Bsp119I TTCGAA 1 cut(s) 819
Bsp1286I GDGCHC 5 cut(s) 147, 744, 994, 1536, 1836
Bsp13I TCCGGA 1 cut(s) 1810
Bsp1407I TGTACA 1 cut(s) 2893
Bsp19I CCATGG 2 cut(s) 1936, 1962
Bsp68I TCGCGA 1 cut(s) 393
BspACI CCGC 5 cut(s) 1262, 1699, 2018, 2231, 2771
BspANI GGCC 5 cut(s) 366, 1941, 1961, 2014, 2838
BspCNI CTCAG 3 cut(s) 126, 737, 1749
BspEI TCCGGA 1 cut(s) 1810
BspFNI CGCG 1 cut(s) 393
BspLI GGNNCC 5 cut(s) 881, 1101, 1662, 2509, 2510
BspMAI CTGCAG 1 cut(s) 831
BspPI GGATC 4 cut(s) 722, 1291, 2387, 2921
BspT104I TTCGAA 1 cut(s) 819
BspTNI GGTCTC 1 cut(s) 1172
BsrDI GCAATG 3 cut(s) 396, 1684, 2470
BsrGI TGTACA 1 cut(s) 2893
BsrI ACTGG 1 cut(s) 42
BssECI CCNNGG 6 cut(s) 772, 1656, 1936, 1956, 1962, 2759
BssT1I CCWWGG 5 cut(s) 772, 1656, 1936, 1962, 2759
Bst2UI CCWGG 1 cut(s) 689
Bst4CI ACNGT 2 cut(s) 1512, 2798
Bst6I CTCTTC 1 cut(s) 1592
BstAPI GCANNNNNTGC 2 cut(s) 440, 1918
BstAUI TGTACA 1 cut(s) 2893
BstBI TTCGAA 1 cut(s) 819
BstC8I GCNNGC 2 cut(s) 769, 1982
BstDEI CTNAG 8 cut(s) 113, 318, 724, 865, 1271, 1736, 1998, 2183
BstDSI CCRYGG 2 cut(s) 1936, 1962
BstENI CCTNNNNNAGG 1 cut(s) 624
BstF5I GGATG 7 cut(s) 247, 615, 636, 759, 1380, 2080, 2862
BstFNI CGCG 1 cut(s) 393
BstMAI GTCTC 6 cut(s) 111, 727, 1172, 1739, 2151, 2510
BstMWI GCNNNNNNNGC 7 cut(s) 137, 440, 1058, 1918, 2026, 2228, 2237
BstNI CCWGG 1 cut(s) 689
BstNSI RCATGY 1 cut(s) 574
BstSCI CCNGG 2 cut(s) 687, 2838
BstSFI CTRYAG 1 cut(s) 827
BstSLI GKGCMC 2 cut(s) 1536, 1836
BstUI CGCG 1 cut(s) 393
BstV1I GCAGC 4 cut(s) 1996, 2206, 2409, 2760
BstV2I GAAGAC 2 cut(s) 2744, 2771
BstX2I RGATCY 3 cut(s) 415, 727, 2068
BstXI CCANNNNNNTGG 2 cut(s) 226, 985
BstYI RGATCY 3 cut(s) 415, 727, 2068
BsuI GTATCC 1 cut(s) 483
BsuRI GGCC 5 cut(s) 366, 1941, 1961, 2014, 2838
BtgI CCRYGG 2 cut(s) 1936, 1962
BtgZI GCGATG 2 cut(s) 2661, 2857
BtrI CACGTC 2 cut(s) 214, 235
BtsCI GGATG 7 cut(s) 247, 615, 636, 759, 1380, 2080, 2862
BtsI GCAGTG 1 cut(s) 2741
BtsIMutI CAGTG 3 cut(s) 252, 1517, 2741
BtuMI TCGCGA 1 cut(s) 393
Cac8I GCNNGC 2 cut(s) 769, 1982
Cfr13I GGNCC 6 cut(s) 1661, 1701, 2418, 2508, 2837, 2847
CseI GACGC 1 cut(s) 2071
Csp6I GTAC 7 cut(s) 62, 486, 2319, 2407, 2566, 2722, 2894
CviQI GTAC 7 cut(s) 62, 486, 2319, 2407, 2566, 2722, 2894
DdeI CTNAG 8 cut(s) 113, 318, 724, 865, 1271, 1736, 1998, 2183
EaeI YGGCCR 2 cut(s) 364, 2012
Eam1104I CTCTTC 1 cut(s) 1592
EarI CTCTTC 1 cut(s) 1592
Ecl136II GAGCTC 1 cut(s) 742
Eco130I CCWWGG 5 cut(s) 772, 1656, 1936, 1962, 2759
Eco24I GRGCYC 1 cut(s) 744
Eco31I GGTCTC 1 cut(s) 1172
Eco47I GGWCC 5 cut(s) 1661, 1701, 2418, 2508, 2847
Eco53kI GAGCTC 1 cut(s) 742
Eco57I CTGAAG 8 cut(s) 27, 284, 615, 1892, 2042, 2156, 2756, 2762
Eco88I CYCGRG 2 cut(s) 1955, 2612
EcoICRI GAGCTC 1 cut(s) 742
EcoNI CCTNNNNNAGG 1 cut(s) 624
EcoO109I RGGNCCY 2 cut(s) 1661, 2508
EcoRII CCWGG 1 cut(s) 687
EcoT14I CCWWGG 5 cut(s) 772, 1656, 1936, 1962, 2759
EcoT22I ATGCAT 2 cut(s) 341, 1524
EcoT38I GRGCYC 1 cut(s) 744
ErhI CCWWGG 5 cut(s) 772, 1656, 1936, 1962, 2759
FalI AAGNNNNNCTT 2 cut(s) 1374, 1406
FaqI GGGAC 4 cut(s) 224, 1674, 2289, 2494
FbaI TGATCA 2 cut(s) 2575, 2787
FblI GTMKAC 1 cut(s) 2056
Fnu4HI GCNGC 4 cut(s) 1985, 2220, 2398, 2774
FokI GGATG 7 cut(s) 254, 602, 643, 746, 1387, 2087, 2849
FriOI GRGCYC 1 cut(s) 744
Fsp4HI GCNGC 4 cut(s) 1985, 2220, 2398, 2774
FspBI CTAG 5 cut(s) 29, 665, 806, 2066, 2279
GluI GCNGC 4 cut(s) 1985, 2220, 2398, 2774
HaeIII GGCC 5 cut(s) 366, 1941, 1961, 2014, 2838
HapII CCGG 2 cut(s) 1811, 2840
HgaI GACGC 1 cut(s) 2071
HincII GTYRAC 2 cut(s) 56, 1974
HindII GTYRAC 2 cut(s) 56, 1974
HindIII AAGCTT 2 cut(s) 1625, 2180
HinfI GANTC 7 cut(s) 179, 639, 684, 1436, 2136, 2369, 2948
HpaII CCGG 2 cut(s) 1811, 2840
HphI GGTGA 9 cut(s) 40, 1604, 2105, 2111, 2117, 2123, 2260, 2642, 2670
Hpy166II GTNNAC 7 cut(s) 56, 568, 1534, 1834, 1974, 2057, 2251
Hpy188I TCNGA 9 cut(s) 116, 128, 264, 727, 1858, 2141, 2604, 2781, 2815
Hpy188III TCNNGA 7 cut(s) 308, 392, 593, 1474, 1811, 2072, 2366
Hpy8I GTNNAC 7 cut(s) 56, 568, 1534, 1834, 1974, 2057, 2251
Hpy99I CGWCG 1 cut(s) 393
HpyCH4III ACNGT 2 cut(s) 1512, 2798
HpyCH4IV ACGT 7 cut(s) 213, 234, 1303, 1428, 1646, 1693, 1775
HpyF10VI GCNNNNNNNGC 7 cut(s) 137, 440, 1058, 1918, 2026, 2228, 2237
HpyF3I CTNAG 8 cut(s) 113, 318, 724, 865, 1271, 1736, 1998, 2183
HpySE526I ACGT 7 cut(s) 213, 234, 1303, 1428, 1646, 1693, 1775
KflI GGGWCCC 1 cut(s) 2508
Kpn2I TCCGGA 1 cut(s) 1810
Ksp22I TGATCA 2 cut(s) 2575, 2787
LmnI GCTCC 2 cut(s) 989, 1243
Lsp1109I GCAGC 4 cut(s) 1996, 2206, 2409, 2760
LweI GCATC 5 cut(s) 142, 799, 1996, 2218, 2653
MaeI CTAG 5 cut(s) 29, 665, 806, 2066, 2279
MaeII ACGT 7 cut(s) 213, 234, 1303, 1428, 1646, 1693, 1775
MfeI CAATTG 3 cut(s) 2037, 2413, 2877
MflI RGATCY 3 cut(s) 415, 727, 2068
MhlI GDGCHC 5 cut(s) 147, 744, 994, 1536, 1836
MlsI TGGCCA 2 cut(s) 366, 2014
MluNI TGGCCA 2 cut(s) 366, 2014
MlyI GAGTC 1 cut(s) 693
MmeI TCCRAC 6 cut(s) 219, 243, 1282, 1324, 1411, 2536
Mox20I TGGCCA 2 cut(s) 366, 2014
Mph1103I ATGCAT 2 cut(s) 341, 1524
MroI TCCGGA 1 cut(s) 1810
MroXI GAANNNNTTC 1 cut(s) 1806
MscI TGGCCA 2 cut(s) 366, 2014
MslI CAYNNNNRTG 2 cut(s) 983, 1992
Msp20I TGGCCA 2 cut(s) 366, 2014
MspA1I CMGCKG 2 cut(s) 2020, 2773
MspI CCGG 2 cut(s) 1811, 2840
MspR9I CCNGG 2 cut(s) 689, 2840
MunI CAATTG 3 cut(s) 2037, 2413, 2877
Mva1269I GAATGC 4 cut(s) 339, 1571, 2347, 2648
MvaI CCWGG 1 cut(s) 689
MvnI CGCG 1 cut(s) 393
MwoI GCNNNNNNNGC 7 cut(s) 137, 440, 1058, 1918, 2026, 2228, 2237
NciI CCSGG 1 cut(s) 2840
NcoI CCATGG 2 cut(s) 1936, 1962
NlaIV GGNNCC 5 cut(s) 881, 1101, 1662, 2509, 2510
NmeAIII GCCGAG 1 cut(s) 1766
NmuCI GTSAC 6 cut(s) 800, 952, 1025, 1321, 1787, 2676
NruI TCGCGA 1 cut(s) 393
NsiI ATGCAT 2 cut(s) 341, 1524
NspI RCATGY 1 cut(s) 574
NspV TTCGAA 1 cut(s) 819
PacI TTAATTAA 1 cut(s) 1347
PaeR7I CTCGAG 1 cut(s) 2612
PciI ACATGT 1 cut(s) 570
PcsI WCGNNNNNNNCGW 3 cut(s) 219, 1821, 2388
PctI GAATGC 4 cut(s) 339, 1571, 2347, 2648
PdmI GAANNNNTTC 1 cut(s) 1806
PfeI GAWTC 6 cut(s) 179, 639, 1436, 2136, 2369, 2948
PflMI CCANNNNNTGG 1 cut(s) 482
PfoI TCCNGGA 1 cut(s) 687
PkrI GCNGC 4 cut(s) 1986, 2221, 2399, 2775
PleI GAGTC 1 cut(s) 692
PpsI GAGTC 1 cut(s) 692
PpuMI RGGWCCY 2 cut(s) 1661, 2508
PscI ACATGT 1 cut(s) 570
Psp124BI GAGCTC 1 cut(s) 744
Psp1406I AACGTT 2 cut(s) 1303, 1646
Psp5II RGGWCCY 2 cut(s) 1661, 2508
Psp6I CCWGG 1 cut(s) 687
PspGI CCWGG 1 cut(s) 687
PspN4I GGNNCC 5 cut(s) 881, 1101, 1662, 2509, 2510
PspPI GGNCC 6 cut(s) 1661, 1701, 2418, 2508, 2837, 2847
PspPPI RGGWCCY 2 cut(s) 1661, 2508
PspXI VCTCGAGB 1 cut(s) 2612
PstI CTGCAG 1 cut(s) 831
PsuI RGATCY 3 cut(s) 415, 727, 2068
RruI TCGCGA 1 cut(s) 393
RsaI GTAC 7 cut(s) 63, 487, 2320, 2408, 2567, 2723, 2895
RsaNI GTAC 7 cut(s) 62, 486, 2319, 2407, 2566, 2722, 2894
RseI CAYNNNNRTG 2 cut(s) 983, 1992
SacI GAGCTC 1 cut(s) 744
SatI GCNGC 4 cut(s) 1985, 2220, 2398, 2774
Sau96I GGNCC 6 cut(s) 1661, 1701, 2418, 2508, 2837, 2847
ScaI AGTACT 2 cut(s) 2567, 2723
SchI GAGTC 1 cut(s) 693
ScrFI CCNGG 2 cut(s) 689, 2840
SduI GDGCHC 5 cut(s) 147, 744, 994, 1536, 1836
SfaNI GCATC 5 cut(s) 142, 799, 1996, 2218, 2653
SfcI CTRYAG 1 cut(s) 827
Sfr274I CTCGAG 1 cut(s) 2612
SfuI TTCGAA 1 cut(s) 819
SinI GGWCC 5 cut(s) 1661, 1701, 2418, 2508, 2847
SlaI CTCGAG 1 cut(s) 2612
SmiMI CAYNNNNRTG 2 cut(s) 983, 1992
SmlI CTYRAG 3 cut(s) 1837, 2513, 2612
SmoI CTYRAG 3 cut(s) 1837, 2513, 2612
SsiI CCGC 5 cut(s) 1262, 1699, 2018, 2231, 2771
SspI AATATT 2 cut(s) 1528, 1550
SspMI CTAG 5 cut(s) 29, 665, 806, 2066, 2279
SstI GAGCTC 1 cut(s) 744
StyD4I CCNGG 2 cut(s) 687, 2838
StyI CCWWGG 5 cut(s) 772, 1656, 1936, 1962, 2759
TaaI ACNGT 2 cut(s) 1512, 2798
TaiI ACGT 7 cut(s) 216, 237, 1306, 1431, 1649, 1696, 1778
TaqI TCGA 9 cut(s) 376, 525, 797, 819, 1800, 2325, 2382, 2613, 2718
TatI WGTACW 3 cut(s) 2565, 2721, 2893
TfiI GAWTC 6 cut(s) 179, 639, 1436, 2136, 2369, 2948
TscAI CASTG 3 cut(s) 259, 1517, 2748
TseFI GTSAC 6 cut(s) 800, 952, 1025, 1321, 1787, 2676
TseI GCWGC 4 cut(s) 1984, 2219, 2397, 2773
Tsp45I GTSAC 6 cut(s) 800, 952, 1025, 1321, 1787, 2676
TspGWI ACGGA 3 cut(s) 10, 172, 2074
TspRI CASTG 3 cut(s) 259, 1517, 2748
Van91I CCANNNNNTGG 1 cut(s) 482
VneI GTGCAC 2 cut(s) 1532, 1832
VpaK11BI GGWCC 5 cut(s) 1661, 1701, 2418, 2508, 2847
XagI CCTNNNNNAGG 1 cut(s) 624
XapI RAATTY 9 cut(s) 258, 457, 694, 1092, 1477, 1493, 1780, 2356, 2782
XceI RCATGY 1 cut(s) 574
XhoI CTCGAG 1 cut(s) 2612
XmiI GTMKAC 1 cut(s) 2056
XmnI GAANNNNTTC 1 cut(s) 1806
XspI CTAG 5 cut(s) 29, 665, 806, 2066, 2279
ZrmI AGTACT 2 cut(s) 2567, 2723
Zsp2I ATGCAT 2 cut(s) 341, 1524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.