FvH4_6g35300

2Fe-2S iron-sulfur cluster binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0009702
Physical Location & Seq
Reverse (-)
27881789 .. 27884353
2565 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g35300.t2

Sequence Viewer

Length: 537 bp
ATGCTTTTTTCTAGATTTTCGGGCCTCGGCAAGCAGATGATCAAGTATCTCACAAAAGGTCCATTGACGTTTTCACCAAAGACAGGGAACGTACATGCAAGTTGCAATTATTTTTCCTGTACTTCGAAGCCTCATTCTTTCTCTACAACAACTGCGCATGAGGAAAGGAACAATCAAGCAGTAGAAGAAATATCTGTAATATTTGTTGATAACGATAGGGAGAAGCATGTTATGGTTCCTGTTGGAATGTCTATACTAGAAGCTGCTCATGAACATGATATTGAACTTGAAGGAGCATGTGAAGGATCTTGTGCTTGTTCTACATGCCATGTGATAGTGAGAGATATGGAGTACTACAACAAACTGGAAGATCCCACTGATGAGGAAAATGACATGCTGGACCTTGCATTCGGCCTCACAGAGACGTCCCGTCTTGGTTGTCAAGTGATAGCCACACCTGAACTCGATGGAATTTGCCTTGAACTGCCCGCTGCCACACGGAACTTTGCCGTTGACAAGCATGCCAGAGCCACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer2

Protein Analysis

179

Amino Acids

19.69

Weight (kDa)

5.0

Isoelectric Point (pI)

43.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fer2 PF00111 70 - 159 4.5e-08 2Fe-2S iron-sulfur cluster binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 428
Acc16I TGCGCA 1 cut(s) 156
AciI CCGC 1 cut(s) 489
AclWI GGATC 2 cut(s) 313, 365
AcsI RAATTY 1 cut(s) 471
AcyI GRCGYC 1 cut(s) 425
AfaI GTAC 3 cut(s) 93, 121, 353
AfiI CCNNNNNNNGG 1 cut(s) 83
AgsI TTSAA 3 cut(s) 284, 290, 482
AhdI GACNNNNNGTC 1 cut(s) 429
AluBI AGCT 1 cut(s) 263
AluI AGCT 1 cut(s) 263
Alw26I GTCTC 1 cut(s) 416
AlwI GGATC 2 cut(s) 313, 365
AoxI GGCC 2 cut(s) 22, 412
ApeKI GCWGC 2 cut(s) 263, 491
ApoI RAATTY 1 cut(s) 471
ArsI GACNNNNNNTTYG 2 cut(s) 392, 424
AspLEI GCGC 1 cut(s) 157
AspS9I GGNCC 3 cut(s) 22, 59, 400
AsuHPI GGTGA 1 cut(s) 66
AsuII TTCGAA 1 cut(s) 125
AvaII GGWCC 2 cut(s) 59, 400
BbvI GCAGC 2 cut(s) 250, 478
BccI CCATC 1 cut(s) 461
BceAI ACGGC 1 cut(s) 494
BclI TGATCA 1 cut(s) 39
BcoDI GTCTC 1 cut(s) 416
BfaI CTAG 2 cut(s) 12, 257
BisI GCNGC 2 cut(s) 264, 492
BlsI GCNGC 2 cut(s) 265, 493
BmcAI AGTACT 1 cut(s) 353
Bme18I GGWCC 2 cut(s) 59, 400
BmeRI GACNNNNNGTC 1 cut(s) 429
BmgT120I GGNCC 3 cut(s) 22, 59, 400
BmiI GGNNCC 1 cut(s) 237
Bpu14I TTCGAA 1 cut(s) 125
BsaHI GRCGYC 1 cut(s) 425
BsaJI CCNNGG 1 cut(s) 25
Bsc4I CCNNNNNNNGG 1 cut(s) 83
Bse1I ACTGG 1 cut(s) 369
BseDI CCNNGG 1 cut(s) 25
BseLI CCNNNNNNNGG 1 cut(s) 83
BseNI ACTGG 1 cut(s) 369
BseXI GCAGC 2 cut(s) 250, 478
BshFI GGCC 2 cut(s) 24, 414
BslFI GGGAC 1 cut(s) 412
BslI CCNNNNNNNGG 1 cut(s) 83
BsmAI GTCTC 1 cut(s) 416
BsmBI CGTCTC 1 cut(s) 416
BsmFI GGGAC 1 cut(s) 412
BsmI GAATGC 1 cut(s) 407
BsnI GGCC 2 cut(s) 24, 414
Bsp119I TTCGAA 1 cut(s) 125
Bsp143I GATC 3 cut(s) 39, 305, 370
BspACI CCGC 1 cut(s) 489
BspANI GGCC 2 cut(s) 24, 414
BspHI TCATGA 1 cut(s) 268
BspLI GGNNCC 1 cut(s) 237
BspPI GGATC 2 cut(s) 313, 365
BspT104I TTCGAA 1 cut(s) 125
BsrI ACTGG 1 cut(s) 369
BssECI CCNNGG 1 cut(s) 25
BssMI GATC 3 cut(s) 39, 305, 370
BssNI GRCGYC 1 cut(s) 425
BstACI GRCGYC 1 cut(s) 425
BstBI TTCGAA 1 cut(s) 125
BstC8I GCNNGC 3 cut(s) 32, 489, 522
BstDEI CTNAG 1 cut(s) 534
BstHHI GCGC 1 cut(s) 157
BstKTI GATC 3 cut(s) 42, 308, 373
BstMAI GTCTC 1 cut(s) 416
BstMBI GATC 3 cut(s) 39, 305, 370
BstNSI RCATGY 6 cut(s) 98, 230, 300, 327, 397, 524
BstV1I GCAGC 2 cut(s) 250, 478
BstX2I RGATCY 2 cut(s) 305, 370
BstYI RGATCY 2 cut(s) 305, 370
BsuRI GGCC 2 cut(s) 24, 414
BtsIMutI CAGTG 1 cut(s) 375
Cac8I GCNNGC 3 cut(s) 32, 489, 522
CciI TCATGA 1 cut(s) 268
CfoI GCGC 1 cut(s) 157
Cfr13I GGNCC 3 cut(s) 22, 59, 400
Csp6I GTAC 3 cut(s) 92, 120, 352
CviJI RGCY 6 cut(s) 24, 130, 263, 414, 452, 530
CviKI_1 RGCY 6 cut(s) 24, 130, 263, 414, 452, 530
CviQI GTAC 3 cut(s) 92, 120, 352
DdeI CTNAG 1 cut(s) 534
DpnI GATC 3 cut(s) 41, 307, 372
DpnII GATC 3 cut(s) 39, 305, 370
DriI GACNNNNNGTC 1 cut(s) 429
Eam1105I GACNNNNNGTC 1 cut(s) 429
Eco47I GGWCC 2 cut(s) 59, 400
Esp3I CGTCTC 1 cut(s) 416
FaqI GGGAC 1 cut(s) 412
FauI CCCGC 1 cut(s) 496
FbaI TGATCA 1 cut(s) 39
Fnu4HI GCNGC 2 cut(s) 264, 492
Fsp4HI GCNGC 2 cut(s) 264, 492
FspBI CTAG 2 cut(s) 12, 257
FspI TGCGCA 1 cut(s) 156
GlaI GCGC 1 cut(s) 156
GluI GCNGC 2 cut(s) 264, 492
HaeIII GGCC 2 cut(s) 24, 414
HhaI GCGC 1 cut(s) 157
Hin1I GRCGYC 1 cut(s) 425
Hin6I GCGC 1 cut(s) 155
HinP1I GCGC 1 cut(s) 155
HincII GTYRAC 1 cut(s) 514
HindII GTYRAC 1 cut(s) 514
HphI GGTGA 1 cut(s) 66
Hpy166II GTNNAC 1 cut(s) 514
Hpy188III TCNNGA 2 cut(s) 12, 269
Hpy8I GTNNAC 1 cut(s) 514
HpyAV CCTTC 2 cut(s) 284, 296
HpyCH4IV ACGT 3 cut(s) 68, 90, 425
HpyCH4V TGCA 3 cut(s) 98, 105, 407
HpyF3I CTNAG 1 cut(s) 534
HpySE526I ACGT 3 cut(s) 68, 90, 425
Hsp92I GRCGYC 1 cut(s) 425
HspAI GCGC 1 cut(s) 155
Ksp22I TGATCA 1 cut(s) 39
Kzo9I GATC 3 cut(s) 39, 305, 370
LmnI GCTCC 1 cut(s) 293
LpnPI CCDG 6 cut(s) 69, 130, 252, 350, 383, 471
Lsp1109I GCAGC 2 cut(s) 250, 478
MaeI CTAG 2 cut(s) 12, 257
MaeII ACGT 3 cut(s) 68, 90, 425
MalI GATC 3 cut(s) 41, 307, 372
MboI GATC 3 cut(s) 39, 305, 370
MboII GAAGA 2 cut(s) 197, 380
MflI RGATCY 2 cut(s) 305, 370
MluCI AATT 2 cut(s) 106, 471
MmeI TCCRAC 1 cut(s) 223
MnlI CCTC 5 cut(s) 35, 141, 154, 376, 425
MslI CAYNNNNRTG 1 cut(s) 273
MspA1I CMGCKG 1 cut(s) 491
Mva1269I GAATGC 1 cut(s) 407
NdeII GATC 3 cut(s) 39, 305, 370
NlaIV GGNNCC 1 cut(s) 237
NmeAIII GCCGAG 1 cut(s) 6
NsbI TGCGCA 1 cut(s) 156
NspI RCATGY 6 cut(s) 98, 230, 300, 327, 397, 524
NspV TTCGAA 1 cut(s) 125
PaeI GCATGC 1 cut(s) 524
PagI TCATGA 1 cut(s) 268
PctI GAATGC 1 cut(s) 407
PkrI GCNGC 2 cut(s) 265, 493
PspN4I GGNNCC 1 cut(s) 237
PspPI GGNCC 3 cut(s) 22, 59, 400
PsuI RGATCY 2 cut(s) 305, 370
RsaI GTAC 3 cut(s) 93, 121, 353
RsaNI GTAC 3 cut(s) 92, 120, 352
RseI CAYNNNNRTG 1 cut(s) 273
SatI GCNGC 2 cut(s) 264, 492
Sau3AI GATC 3 cut(s) 39, 305, 370
Sau96I GGNCC 3 cut(s) 22, 59, 400
ScaI AGTACT 1 cut(s) 353
SetI ASST 7 cut(s) 61, 71, 93, 265, 405, 428, 460
SfuI TTCGAA 1 cut(s) 125
SinI GGWCC 2 cut(s) 59, 400
SmiMI CAYNNNNRTG 1 cut(s) 273
SphI GCATGC 1 cut(s) 524
Sse9I AATT 2 cut(s) 106, 471
SsiI CCGC 1 cut(s) 489
SspI AATATT 1 cut(s) 201
SspMI CTAG 2 cut(s) 12, 257
TaiI ACGT 3 cut(s) 71, 93, 428
TaqI TCGA 2 cut(s) 125, 465
TasI AATT 2 cut(s) 106, 471
TatI WGTACW 2 cut(s) 119, 351
TscAI CASTG 1 cut(s) 382
TseI GCWGC 2 cut(s) 263, 491
TspDTI ATGAA 1 cut(s) 285
TspGWI ACGGA 1 cut(s) 514
TspRI CASTG 1 cut(s) 382
VpaK11BI GGWCC 2 cut(s) 59, 400
XapI RAATTY 1 cut(s) 471
XbaI TCTAGA 1 cut(s) 11
XceI RCATGY 6 cut(s) 98, 230, 300, 327, 397, 524
XspI CTAG 2 cut(s) 12, 257
ZraI GACGTC 1 cut(s) 426
ZrmI AGTACT 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.