RLG00000020218

2Fe-2S iron-sulfur cluster binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0009702
Physical Location & Seq
Reverse (-)
62550100 .. 62552157
2058 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020218

Sequence Viewer

Length: 447 bp
ATGATCAAGTATCTCAGAAAAGTCTCATTTTCTTCTCAGACGCCTCGTACTTTTTCTAGAACAACTGCTTACGAGGAAGGGAACAAACAAATAGAAGAAATATCTGTGATATTTGTTGATAACGATGGGGAGAAGCATGTTAAAGTTCCTGTTGGAAAGTCTATACTAGAAGCTGCTCATGAAAATGATATTGAACTTGAAGGAGCATGTGAAGGATCCTGTGCTTGTTCTACATGCCATGTGATTGTGAGAGAATTGGAGTACTACAATAAACTGGAAGATCCCACTGATGAGGAAAATGACATGCTGGACCTTGCATTCGGGCTCACAGAGACGTCTCGCCTGGGTTGTCAAGTGATTGCAACACCTGAACTCGATGGAATATGCCTTGAGCTGCCCGCCGCAACTCGGAACTTTGCTGTCAACAAGTATGCCAAAGCCACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer2

Protein Analysis

149

Amino Acids

16.44

Weight (kDa)

4.69

Isoelectric Point (pI)

51.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fer2 PF00111 40 - 122 3.7e-13 2Fe-2S iron-sulfur cluster binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 338
AciI CCGC 2 cut(s) 399, 402
AclWI GGATC 3 cut(s) 210, 223, 275
AcyI GRCGYC 2 cut(s) 41, 335
AfaI GTAC 2 cut(s) 49, 263
AfiI CCNNNNNNNGG 1 cut(s) 408
AgsI TTSAA 2 cut(s) 194, 200
AjnI CCWGG 1 cut(s) 342
AluBI AGCT 2 cut(s) 173, 394
AluI AGCT 2 cut(s) 173, 394
Alw26I GTCTC 3 cut(s) 28, 326, 342
AlwI GGATC 3 cut(s) 210, 223, 275
ApeKI GCWGC 2 cut(s) 173, 394
ArsI GACNNNNNNTTYG 2 cut(s) 302, 334
AspS9I GGNCC 1 cut(s) 310
AvaII GGWCC 1 cut(s) 310
BamHI GGATCC 1 cut(s) 215
BanII GRGCYC 1 cut(s) 327
BbvI GCAGC 2 cut(s) 160, 381
BccI CCATC 2 cut(s) 119, 371
BciT130I CCWGG 1 cut(s) 344
BclI TGATCA 1 cut(s) 3
BcoDI GTCTC 3 cut(s) 28, 326, 342
BfaI CTAG 3 cut(s) 57, 167, 445
BisI GCNGC 3 cut(s) 174, 395, 402
BlsI GCNGC 3 cut(s) 175, 396, 403
BmcAI AGTACT 1 cut(s) 263
Bme1390I CCNGG 1 cut(s) 344
Bme18I GGWCC 1 cut(s) 310
BmgT120I GGNCC 1 cut(s) 310
BmiI GGNNCC 1 cut(s) 217
BmrFI CCNGG 1 cut(s) 344
BpuEI CTTGAG 1 cut(s) 410
BsaHI GRCGYC 2 cut(s) 41, 335
BsaJI CCNNGG 1 cut(s) 343
Bsc4I CCNNNNNNNGG 1 cut(s) 408
Bse1I ACTGG 1 cut(s) 279
BseBI CCWGG 1 cut(s) 344
BseDI CCNNGG 1 cut(s) 343
BseLI CCNNNNNNNGG 1 cut(s) 408
BseMII CTCAG 2 cut(s) 28, 50
BseNI ACTGG 1 cut(s) 279
BseXI GCAGC 2 cut(s) 160, 381
BslI CCNNNNNNNGG 1 cut(s) 408
BsmAI GTCTC 3 cut(s) 28, 326, 342
BsmBI CGTCTC 2 cut(s) 326, 342
BsmI GAATGC 1 cut(s) 317
Bsp1286I GDGCHC 1 cut(s) 327
Bsp143I GATC 3 cut(s) 3, 215, 280
BspACI CCGC 2 cut(s) 399, 402
BspCNI CTCAG 2 cut(s) 27, 49
BspHI TCATGA 1 cut(s) 178
BspLI GGNNCC 1 cut(s) 217
BspPI GGATC 3 cut(s) 210, 223, 275
BsrI ACTGG 1 cut(s) 279
BssECI CCNNGG 1 cut(s) 343
BssMI GATC 3 cut(s) 3, 215, 280
BssNI GRCGYC 2 cut(s) 41, 335
Bst2UI CCWGG 1 cut(s) 344
BstACI GRCGYC 2 cut(s) 41, 335
BstC8I GCNNGC 1 cut(s) 399
BstDEI CTNAG 2 cut(s) 14, 36
BstKTI GATC 3 cut(s) 6, 218, 283
BstMAI GTCTC 3 cut(s) 28, 326, 342
BstMBI GATC 3 cut(s) 3, 215, 280
BstNI CCWGG 1 cut(s) 344
BstNSI RCATGY 4 cut(s) 140, 210, 237, 307
BstSCI CCNGG 1 cut(s) 342
BstV1I GCAGC 2 cut(s) 160, 381
BstX2I RGATCY 2 cut(s) 215, 280
BstYI RGATCY 2 cut(s) 215, 280
BtsIMutI CAGTG 1 cut(s) 285
Cac8I GCNNGC 1 cut(s) 399
CciI TCATGA 1 cut(s) 178
Cfr13I GGNCC 1 cut(s) 310
CseI GACGC 1 cut(s) 49
Csp6I GTAC 2 cut(s) 48, 262
CviAII CATG 6 cut(s) 137, 179, 207, 234, 239, 304
CviJI RGCY 4 cut(s) 173, 325, 394, 440
CviKI_1 RGCY 4 cut(s) 173, 325, 394, 440
CviQI GTAC 2 cut(s) 48, 262
DdeI CTNAG 2 cut(s) 14, 36
DpnI GATC 3 cut(s) 5, 217, 282
DpnII GATC 3 cut(s) 3, 215, 280
Eco24I GRGCYC 1 cut(s) 327
Eco47I GGWCC 1 cut(s) 310
EcoRII CCWGG 1 cut(s) 342
EcoT38I GRGCYC 1 cut(s) 327
Esp3I CGTCTC 2 cut(s) 326, 342
FaeI CATG 6 cut(s) 140, 182, 210, 237, 242, 307
FaiI YATR 9 cut(s) 138, 164, 180, 208, 235, 240, 305, 385, 432
FatI CATG 6 cut(s) 136, 178, 206, 233, 238, 303
FauI CCCGC 1 cut(s) 406
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 3 cut(s) 174, 395, 402
FriOI GRGCYC 1 cut(s) 327
Fsp4HI GCNGC 3 cut(s) 174, 395, 402
FspBI CTAG 3 cut(s) 57, 167, 445
GluI GCNGC 3 cut(s) 174, 395, 402
HgaI GACGC 1 cut(s) 49
Hin1I GRCGYC 2 cut(s) 41, 335
Hin1II CATG 6 cut(s) 140, 182, 210, 237, 242, 307
HincII GTYRAC 1 cut(s) 424
HindII GTYRAC 1 cut(s) 424
Hpy166II GTNNAC 1 cut(s) 424
Hpy188I TCNGA 3 cut(s) 17, 39, 411
Hpy188III TCNNGA 2 cut(s) 57, 179
Hpy8I GTNNAC 1 cut(s) 424
HpyAV CCTTC 3 cut(s) 71, 194, 206
HpyCH4IV ACGT 1 cut(s) 335
HpyCH4V TGCA 2 cut(s) 317, 362
HpyF3I CTNAG 2 cut(s) 14, 36
HpySE526I ACGT 1 cut(s) 335
Hsp92I GRCGYC 2 cut(s) 41, 335
Hsp92II CATG 6 cut(s) 140, 182, 210, 237, 242, 307
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 3 cut(s) 3, 215, 280
LmnI GCTCC 1 cut(s) 203
LpnPI CCDG 7 cut(s) 162, 232, 260, 293, 329, 356, 381
Lsp1109I GCAGC 2 cut(s) 160, 381
MaeI CTAG 3 cut(s) 57, 167, 445
MaeII ACGT 1 cut(s) 335
MalI GATC 3 cut(s) 5, 217, 282
MboI GATC 3 cut(s) 3, 215, 280
MboII GAAGA 3 cut(s) 24, 107, 290
MflI RGATCY 2 cut(s) 215, 280
MhlI GDGCHC 1 cut(s) 327
MluCI AATT 1 cut(s) 254
MmeI TCCRAC 1 cut(s) 133
MnlI CCTC 3 cut(s) 54, 67, 286
MseI TTAA 1 cut(s) 141
MslI CAYNNNNRTG 1 cut(s) 183
MspR9I CCNGG 1 cut(s) 344
Mva1269I GAATGC 1 cut(s) 317
MvaI CCWGG 1 cut(s) 344
NdeII GATC 3 cut(s) 3, 215, 280
NlaIII CATG 6 cut(s) 140, 182, 210, 237, 242, 307
NlaIV GGNNCC 1 cut(s) 217
NspI RCATGY 4 cut(s) 140, 210, 237, 307
PagI TCATGA 1 cut(s) 178
PctI GAATGC 1 cut(s) 317
PkrI GCNGC 3 cut(s) 175, 396, 403
Psp6I CCWGG 1 cut(s) 342
PspGI CCWGG 1 cut(s) 342
PspN4I GGNNCC 1 cut(s) 217
PspPI GGNCC 1 cut(s) 310
PsuI RGATCY 2 cut(s) 215, 280
RsaI GTAC 2 cut(s) 49, 263
RsaNI GTAC 2 cut(s) 48, 262
RseI CAYNNNNRTG 1 cut(s) 183
SaqAI TTAA 1 cut(s) 141
SatI GCNGC 3 cut(s) 174, 395, 402
Sau3AI GATC 3 cut(s) 3, 215, 280
Sau96I GGNCC 1 cut(s) 310
ScaI AGTACT 1 cut(s) 263
ScrFI CCNGG 1 cut(s) 344
SduI GDGCHC 1 cut(s) 327
SetI ASST 6 cut(s) 175, 315, 338, 370, 396, 446
SinI GGWCC 1 cut(s) 310
SmiMI CAYNNNNRTG 1 cut(s) 183
SmlI CTYRAG 1 cut(s) 389
SmoI CTYRAG 1 cut(s) 389
Sse9I AATT 1 cut(s) 254
SsiI CCGC 2 cut(s) 399, 402
SspMI CTAG 3 cut(s) 57, 167, 445
StyD4I CCNGG 1 cut(s) 342
TaiI ACGT 1 cut(s) 338
TaqI TCGA 1 cut(s) 375
TasI AATT 1 cut(s) 254
TatI WGTACW 1 cut(s) 261
TauI GCSGC 1 cut(s) 404
Tru1I TTAA 1 cut(s) 141
Tru9I TTAA 1 cut(s) 141
TscAI CASTG 1 cut(s) 292
TseI GCWGC 2 cut(s) 173, 394
TspDTI ATGAA 1 cut(s) 195
TspRI CASTG 1 cut(s) 292
VpaK11BI GGWCC 1 cut(s) 310
XbaI TCTAGA 1 cut(s) 56
XceI RCATGY 4 cut(s) 140, 210, 237, 307
XspI CTAG 3 cut(s) 57, 167, 445
ZraI GACGTC 1 cut(s) 336
ZrmI AGTACT 1 cut(s) 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.