Rh2DG474500

2Fe-2S iron-sulfur cluster binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0009702
Physical Location & Seq
Reverse (-)
68662341 .. 68664498
2158 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG474500.1

Sequence Viewer

Length: 531 bp
ATGGTTGTTTCTAGATTTTCGGGCCTCGGCAGGCAGATGATCAAGTATCTCAGAAAAGGTCCATTGACATTTTCACCAAAGGCAGGAAACTTATGTGCAAGTTACAATTATTTTTCCCATACTTTGACGCCTCGTACTTTTTCTACAACTGCTCATGAGGAAGGGAACAAACAAATAGAAGAAATATCTGTGATATTTGTTGATAACGATGGGGAGAAGCATGTTAAAGTTCCTGTTGGAAAGTCTATACTAGAAGCTGCTCATGAAAATGATATTGAACTTGAAGGAGCATGTGAAGGATCCTGTGCTTGTTCTACATGCCATGTGATTGTGAGAGATTTGGAGTACTACAATAAACTGGAAGATCCCACTGATGAGGAAAATGACATGCTGGACCTTGCATTCGGGCTCACAGAGACGTCTCGTCTGGGTTGTCAAGTGATTGCAACACCTGAACTCGATGGAATATGCCTTGAACTGCCCGCAGCCACGCGGAACTTTGCTGTCGACAAGTATGCCAAAGCCACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer2

Protein Analysis

176

Amino Acids

19.4

Weight (kDa)

4.93

Isoelectric Point (pI)

43.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fer2 PF00111 68 - 150 9.2e-13 2Fe-2S iron-sulfur cluster binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 422
AccI GTMKAC 1 cut(s) 507
AccII CGCG 1 cut(s) 493
AciI CCGC 2 cut(s) 483, 493
AclWI GGATC 3 cut(s) 294, 307, 359
AcyI GRCGYC 2 cut(s) 128, 419
AfaI GTAC 2 cut(s) 136, 347
AfiI CCNNNNNNNGG 1 cut(s) 83
AgsI TTSAA 3 cut(s) 278, 284, 476
AhdI GACNNNNNGTC 1 cut(s) 423
AluBI AGCT 1 cut(s) 257
AluI AGCT 1 cut(s) 257
Alw26I GTCTC 2 cut(s) 410, 426
AlwI GGATC 3 cut(s) 294, 307, 359
AoxI GGCC 1 cut(s) 22
ApeKI GCWGC 2 cut(s) 257, 485
ArsI GACNNNNNNTTYG 2 cut(s) 386, 418
AspS9I GGNCC 3 cut(s) 22, 59, 394
AsuHPI GGTGA 1 cut(s) 66
AvaII GGWCC 2 cut(s) 59, 394
BamHI GGATCC 1 cut(s) 299
BanII GRGCYC 1 cut(s) 411
BbvI GCAGC 2 cut(s) 244, 497
BccI CCATC 2 cut(s) 203, 455
BcgI CGANNNNNNTGC 2 cut(s) 497, 531
BclI TGATCA 1 cut(s) 39
BcoDI GTCTC 2 cut(s) 410, 426
BfaI CTAG 3 cut(s) 12, 251, 529
BisI GCNGC 2 cut(s) 258, 486
BlsI GCNGC 2 cut(s) 259, 487
BmcAI AGTACT 1 cut(s) 347
Bme18I GGWCC 2 cut(s) 59, 394
BmeRI GACNNNNNGTC 1 cut(s) 423
BmgT120I GGNCC 3 cut(s) 22, 59, 394
BmiI GGNNCC 1 cut(s) 301
BsaHI GRCGYC 2 cut(s) 128, 419
BsaJI CCNNGG 1 cut(s) 25
Bsc4I CCNNNNNNNGG 1 cut(s) 83
Bse1I ACTGG 1 cut(s) 363
BseDI CCNNGG 1 cut(s) 25
BseLI CCNNNNNNNGG 1 cut(s) 83
BseMII CTCAG 1 cut(s) 64
BseNI ACTGG 1 cut(s) 363
BseXI GCAGC 2 cut(s) 244, 497
Bsh1236I CGCG 1 cut(s) 493
BshFI GGCC 1 cut(s) 24
BslI CCNNNNNNNGG 1 cut(s) 83
BsmAI GTCTC 2 cut(s) 410, 426
BsmBI CGTCTC 2 cut(s) 410, 426
BsmI GAATGC 1 cut(s) 401
BsnI GGCC 1 cut(s) 24
Bsp1286I GDGCHC 1 cut(s) 411
Bsp143I GATC 3 cut(s) 39, 299, 364
BspACI CCGC 2 cut(s) 483, 493
BspANI GGCC 1 cut(s) 24
BspCNI CTCAG 1 cut(s) 63
BspFNI CGCG 1 cut(s) 493
BspHI TCATGA 2 cut(s) 154, 262
BspLI GGNNCC 1 cut(s) 301
BspPI GGATC 3 cut(s) 294, 307, 359
BsrI ACTGG 1 cut(s) 363
BssECI CCNNGG 1 cut(s) 25
BssMI GATC 3 cut(s) 39, 299, 364
BssNI GRCGYC 2 cut(s) 128, 419
BstACI GRCGYC 2 cut(s) 128, 419
BstC8I GCNNGC 2 cut(s) 32, 483
BstDEI CTNAG 1 cut(s) 50
BstFNI CGCG 1 cut(s) 493
BstKTI GATC 3 cut(s) 42, 302, 367
BstMAI GTCTC 2 cut(s) 410, 426
BstMBI GATC 3 cut(s) 39, 299, 364
BstNSI RCATGY 4 cut(s) 224, 294, 321, 391
BstUI CGCG 1 cut(s) 493
BstV1I GCAGC 2 cut(s) 244, 497
BstX2I RGATCY 2 cut(s) 299, 364
BstYI RGATCY 2 cut(s) 299, 364
BsuRI GGCC 1 cut(s) 24
BtsIMutI CAGTG 1 cut(s) 369
Cac8I GCNNGC 2 cut(s) 32, 483
CciI TCATGA 2 cut(s) 154, 262
Cfr13I GGNCC 3 cut(s) 22, 59, 394
CseI GACGC 1 cut(s) 136
Csp6I GTAC 2 cut(s) 135, 346
CviAII CATG 7 cut(s) 155, 221, 263, 291, 318, 323, 388
CviJI RGCY 5 cut(s) 24, 257, 409, 488, 524
CviKI_1 RGCY 5 cut(s) 24, 257, 409, 488, 524
CviQI GTAC 2 cut(s) 135, 346
DdeI CTNAG 1 cut(s) 50
DpnI GATC 3 cut(s) 41, 301, 366
DpnII GATC 3 cut(s) 39, 299, 364
DriI GACNNNNNGTC 1 cut(s) 423
Eam1105I GACNNNNNGTC 1 cut(s) 423
Eco24I GRGCYC 1 cut(s) 411
Eco47I GGWCC 2 cut(s) 59, 394
EcoT38I GRGCYC 1 cut(s) 411
Esp3I CGTCTC 2 cut(s) 410, 426
FaeI CATG 7 cut(s) 158, 224, 266, 294, 321, 326, 391
FatI CATG 7 cut(s) 154, 220, 262, 290, 317, 322, 387
FauI CCCGC 1 cut(s) 490
FbaI TGATCA 1 cut(s) 39
FblI GTMKAC 1 cut(s) 507
Fnu4HI GCNGC 2 cut(s) 258, 486
FriOI GRGCYC 1 cut(s) 411
Fsp4HI GCNGC 2 cut(s) 258, 486
FspBI CTAG 3 cut(s) 12, 251, 529
GluI GCNGC 2 cut(s) 258, 486
HaeIII GGCC 1 cut(s) 24
HgaI GACGC 1 cut(s) 136
Hin1I GRCGYC 2 cut(s) 128, 419
Hin1II CATG 7 cut(s) 158, 224, 266, 294, 321, 326, 391
HincII GTYRAC 1 cut(s) 508
HindII GTYRAC 1 cut(s) 508
HphI GGTGA 1 cut(s) 66
Hpy166II GTNNAC 1 cut(s) 508
Hpy188I TCNGA 1 cut(s) 53
Hpy188III TCNNGA 3 cut(s) 12, 155, 263
Hpy8I GTNNAC 1 cut(s) 508
HpyAV CCTTC 3 cut(s) 155, 278, 290
HpyCH4IV ACGT 1 cut(s) 419
HpyCH4V TGCA 3 cut(s) 98, 401, 446
HpyF3I CTNAG 1 cut(s) 50
HpySE526I ACGT 1 cut(s) 419
Hsp92I GRCGYC 2 cut(s) 128, 419
Hsp92II CATG 7 cut(s) 158, 224, 266, 294, 321, 326, 391
Ksp22I TGATCA 1 cut(s) 39
Kzo9I GATC 3 cut(s) 39, 299, 364
LmnI GCTCC 1 cut(s) 287
LpnPI CCDG 8 cut(s) 16, 69, 246, 316, 344, 377, 413, 465
Lsp1109I GCAGC 2 cut(s) 244, 497
MaeI CTAG 3 cut(s) 12, 251, 529
MaeII ACGT 1 cut(s) 419
MaeIII GTNAC 1 cut(s) 101
MalI GATC 3 cut(s) 41, 301, 366
MboI GATC 3 cut(s) 39, 299, 364
MboII GAAGA 2 cut(s) 191, 374
MflI RGATCY 2 cut(s) 299, 364
MhlI GDGCHC 1 cut(s) 411
MluCI AATT 1 cut(s) 106
MmeI TCCRAC 1 cut(s) 217
MnlI CCTC 4 cut(s) 35, 141, 151, 370
MseI TTAA 1 cut(s) 225
MslI CAYNNNNRTG 1 cut(s) 267
Mva1269I GAATGC 1 cut(s) 401
MvnI CGCG 1 cut(s) 493
NdeII GATC 3 cut(s) 39, 299, 364
NlaIII CATG 7 cut(s) 158, 224, 266, 294, 321, 326, 391
NlaIV GGNNCC 1 cut(s) 301
NmeAIII GCCGAG 1 cut(s) 6
NspI RCATGY 4 cut(s) 224, 294, 321, 391
PagI TCATGA 2 cut(s) 154, 262
PctI GAATGC 1 cut(s) 401
PkrI GCNGC 2 cut(s) 259, 487
PspN4I GGNNCC 1 cut(s) 301
PspPI GGNCC 3 cut(s) 22, 59, 394
PsuI RGATCY 2 cut(s) 299, 364
RsaI GTAC 2 cut(s) 136, 347
RsaNI GTAC 2 cut(s) 135, 346
RseI CAYNNNNRTG 1 cut(s) 267
SalI GTCGAC 1 cut(s) 506
SaqAI TTAA 1 cut(s) 225
SatI GCNGC 2 cut(s) 258, 486
Sau3AI GATC 3 cut(s) 39, 299, 364
Sau96I GGNCC 3 cut(s) 22, 59, 394
ScaI AGTACT 1 cut(s) 347
SduI GDGCHC 1 cut(s) 411
SetI ASST 6 cut(s) 61, 259, 399, 422, 454, 530
SinI GGWCC 2 cut(s) 59, 394
SmiMI CAYNNNNRTG 1 cut(s) 267
Sse9I AATT 1 cut(s) 106
SsiI CCGC 2 cut(s) 483, 493
SspMI CTAG 3 cut(s) 12, 251, 529
TaiI ACGT 1 cut(s) 422
TaqI TCGA 2 cut(s) 459, 507
TasI AATT 1 cut(s) 106
TatI WGTACW 1 cut(s) 345
Tru1I TTAA 1 cut(s) 225
Tru9I TTAA 1 cut(s) 225
TscAI CASTG 1 cut(s) 376
TseI GCWGC 2 cut(s) 257, 485
TspDTI ATGAA 1 cut(s) 279
TspRI CASTG 1 cut(s) 376
VpaK11BI GGWCC 2 cut(s) 59, 394
XbaI TCTAGA 1 cut(s) 11
XceI RCATGY 4 cut(s) 224, 294, 321, 391
XmiI GTMKAC 1 cut(s) 507
XspI CTAG 3 cut(s) 12, 251, 529
ZraI GACGTC 1 cut(s) 420
ZrmI AGTACT 1 cut(s) 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.