RchiOBHm_Chr2g0146741

2Fe-2S iron-sulfur cluster binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0009702
Physical Location & Seq
Reverse (-)
64355362 .. 64357604
2243 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51645

Sequence Viewer

Length: 480 bp
ATGGGTCCATTGACATTTTCACCAAAGGCAGGAAACTTATGTGCAAGTTACAATTATTTTTCCCATACTTTGACGCCTCGTACTTTTTCTACAACAACCGCTCATGAGGAAGGGAACAAACAAATAGAAGAAATATCTGTGATATTTGTTGATAACGATGGGGAGAAGCATGTTAAAGTTCCTGTTGGAAAGTCTATACTAGAAGCTGCTCATGAAAATGATATTGAACTTGAAGGAGCATGTGAAGGATCCTGTGCTTGTTCTACATGCCATGTGATTGTGAGAGATTTGGAGTACTACAATAAACTGGAAGATCCCACTGATGAGGAAAATGACATGCTGGACCTTGCATTCGGGCTCACAGAGACGTCTCGTCTGGGTTGTCAAGTGATTGCAACACCTGAACTCGATGGAATATGCCTTGAACTGCCCGCAGCCACGCGGAACTTTGCTGTCGACAAGTATGCCAAAGCCACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer2

Protein Analysis

159

Amino Acids

17.38

Weight (kDa)

4.54

Isoelectric Point (pI)

44.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fer2 PF00111 51 - 133 7.1e-13 2Fe-2S iron-sulfur cluster binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 371
AccBSI CCGCTC 1 cut(s) 101
AccI GTMKAC 1 cut(s) 456
AccII CGCG 1 cut(s) 442
AciI CCGC 3 cut(s) 99, 432, 442
AclWI GGATC 3 cut(s) 243, 256, 308
AcyI GRCGYC 2 cut(s) 74, 368
AfaI GTAC 2 cut(s) 82, 296
AfiI CCNNNNNNNGG 1 cut(s) 29
AgsI TTSAA 3 cut(s) 227, 233, 425
AhdI GACNNNNNGTC 1 cut(s) 372
AluBI AGCT 1 cut(s) 206
AluI AGCT 1 cut(s) 206
Alw26I GTCTC 2 cut(s) 359, 375
AlwI GGATC 3 cut(s) 243, 256, 308
ApeKI GCWGC 2 cut(s) 206, 434
ArsI GACNNNNNNTTYG 2 cut(s) 335, 367
AspS9I GGNCC 2 cut(s) 5, 343
AsuHPI GGTGA 1 cut(s) 12
AvaII GGWCC 2 cut(s) 5, 343
BamHI GGATCC 1 cut(s) 248
BanII GRGCYC 1 cut(s) 360
BbvI GCAGC 2 cut(s) 193, 446
BccI CCATC 2 cut(s) 152, 404
BcgI CGANNNNNNTGC 2 cut(s) 446, 480
BcoDI GTCTC 2 cut(s) 359, 375
BfaI CTAG 2 cut(s) 200, 478
BisI GCNGC 2 cut(s) 207, 435
BlsI GCNGC 2 cut(s) 208, 436
BmcAI AGTACT 1 cut(s) 296
Bme18I GGWCC 2 cut(s) 5, 343
BmeRI GACNNNNNGTC 1 cut(s) 372
BmgT120I GGNCC 2 cut(s) 5, 343
BmiI GGNNCC 2 cut(s) 6, 250
BsaHI GRCGYC 2 cut(s) 74, 368
Bsc4I CCNNNNNNNGG 1 cut(s) 29
Bse1I ACTGG 1 cut(s) 312
BseLI CCNNNNNNNGG 1 cut(s) 29
BseNI ACTGG 1 cut(s) 312
BseXI GCAGC 2 cut(s) 193, 446
Bsh1236I CGCG 1 cut(s) 442
BslI CCNNNNNNNGG 1 cut(s) 29
BsmAI GTCTC 2 cut(s) 359, 375
BsmBI CGTCTC 2 cut(s) 359, 375
BsmI GAATGC 1 cut(s) 350
Bsp1286I GDGCHC 1 cut(s) 360
Bsp143I GATC 2 cut(s) 248, 313
BspACI CCGC 3 cut(s) 99, 432, 442
BspFNI CGCG 1 cut(s) 442
BspHI TCATGA 2 cut(s) 103, 211
BspLI GGNNCC 2 cut(s) 6, 250
BspPI GGATC 3 cut(s) 243, 256, 308
BsrBI CCGCTC 1 cut(s) 101
BsrI ACTGG 1 cut(s) 312
BssMI GATC 2 cut(s) 248, 313
BssNI GRCGYC 2 cut(s) 74, 368
BstACI GRCGYC 2 cut(s) 74, 368
BstC8I GCNNGC 1 cut(s) 432
BstFNI CGCG 1 cut(s) 442
BstKTI GATC 2 cut(s) 251, 316
BstMAI GTCTC 2 cut(s) 359, 375
BstMBI GATC 2 cut(s) 248, 313
BstNSI RCATGY 4 cut(s) 173, 243, 270, 340
BstUI CGCG 1 cut(s) 442
BstV1I GCAGC 2 cut(s) 193, 446
BstX2I RGATCY 2 cut(s) 248, 313
BstYI RGATCY 2 cut(s) 248, 313
BtsIMutI CAGTG 1 cut(s) 318
Cac8I GCNNGC 1 cut(s) 432
CciI TCATGA 2 cut(s) 103, 211
Cfr13I GGNCC 2 cut(s) 5, 343
CseI GACGC 1 cut(s) 82
Csp6I GTAC 2 cut(s) 81, 295
CviAII CATG 7 cut(s) 104, 170, 212, 240, 267, 272, 337
CviJI RGCY 4 cut(s) 206, 358, 437, 473
CviKI_1 RGCY 4 cut(s) 206, 358, 437, 473
CviQI GTAC 2 cut(s) 81, 295
DpnI GATC 2 cut(s) 250, 315
DpnII GATC 2 cut(s) 248, 313
DriI GACNNNNNGTC 1 cut(s) 372
Eam1105I GACNNNNNGTC 1 cut(s) 372
Eco24I GRGCYC 1 cut(s) 360
Eco47I GGWCC 2 cut(s) 5, 343
EcoT38I GRGCYC 1 cut(s) 360
Esp3I CGTCTC 2 cut(s) 359, 375
FaeI CATG 7 cut(s) 107, 173, 215, 243, 270, 275, 340
FatI CATG 7 cut(s) 103, 169, 211, 239, 266, 271, 336
FauI CCCGC 1 cut(s) 439
FblI GTMKAC 1 cut(s) 456
Fnu4HI GCNGC 2 cut(s) 207, 435
FriOI GRGCYC 1 cut(s) 360
Fsp4HI GCNGC 2 cut(s) 207, 435
FspBI CTAG 2 cut(s) 200, 478
GluI GCNGC 2 cut(s) 207, 435
HgaI GACGC 1 cut(s) 82
Hin1I GRCGYC 2 cut(s) 74, 368
Hin1II CATG 7 cut(s) 107, 173, 215, 243, 270, 275, 340
HincII GTYRAC 1 cut(s) 457
HindII GTYRAC 1 cut(s) 457
HphI GGTGA 1 cut(s) 12
Hpy166II GTNNAC 1 cut(s) 457
Hpy188III TCNNGA 2 cut(s) 104, 212
Hpy8I GTNNAC 1 cut(s) 457
HpyAV CCTTC 3 cut(s) 104, 227, 239
HpyCH4IV ACGT 1 cut(s) 368
HpyCH4V TGCA 3 cut(s) 44, 350, 395
HpySE526I ACGT 1 cut(s) 368
Hsp92I GRCGYC 2 cut(s) 74, 368
Hsp92II CATG 7 cut(s) 107, 173, 215, 243, 270, 275, 340
Kzo9I GATC 2 cut(s) 248, 313
LmnI GCTCC 1 cut(s) 236
LpnPI CCDG 7 cut(s) 15, 195, 265, 293, 326, 362, 414
Lsp1109I GCAGC 2 cut(s) 193, 446
MaeI CTAG 2 cut(s) 200, 478
MaeII ACGT 1 cut(s) 368
MaeIII GTNAC 1 cut(s) 47
MalI GATC 2 cut(s) 250, 315
MbiI CCGCTC 1 cut(s) 101
MboI GATC 2 cut(s) 248, 313
MboII GAAGA 2 cut(s) 140, 323
MflI RGATCY 2 cut(s) 248, 313
MhlI GDGCHC 1 cut(s) 360
MluCI AATT 1 cut(s) 52
MmeI TCCRAC 1 cut(s) 166
MnlI CCTC 3 cut(s) 87, 100, 319
MseI TTAA 1 cut(s) 174
MslI CAYNNNNRTG 1 cut(s) 216
Mva1269I GAATGC 1 cut(s) 350
MvnI CGCG 1 cut(s) 442
NdeII GATC 2 cut(s) 248, 313
NlaIII CATG 7 cut(s) 107, 173, 215, 243, 270, 275, 340
NlaIV GGNNCC 2 cut(s) 6, 250
NspI RCATGY 4 cut(s) 173, 243, 270, 340
PagI TCATGA 2 cut(s) 103, 211
PctI GAATGC 1 cut(s) 350
PkrI GCNGC 2 cut(s) 208, 436
PspN4I GGNNCC 2 cut(s) 6, 250
PspPI GGNCC 2 cut(s) 5, 343
PsuI RGATCY 2 cut(s) 248, 313
RsaI GTAC 2 cut(s) 82, 296
RsaNI GTAC 2 cut(s) 81, 295
RseI CAYNNNNRTG 1 cut(s) 216
SalI GTCGAC 1 cut(s) 455
SaqAI TTAA 1 cut(s) 174
SatI GCNGC 2 cut(s) 207, 435
Sau3AI GATC 2 cut(s) 248, 313
Sau96I GGNCC 2 cut(s) 5, 343
ScaI AGTACT 1 cut(s) 296
SduI GDGCHC 1 cut(s) 360
SetI ASST 5 cut(s) 208, 348, 371, 403, 479
SinI GGWCC 2 cut(s) 5, 343
SmiMI CAYNNNNRTG 1 cut(s) 216
Sse9I AATT 1 cut(s) 52
SsiI CCGC 3 cut(s) 99, 432, 442
SspMI CTAG 2 cut(s) 200, 478
TaiI ACGT 1 cut(s) 371
TaqI TCGA 2 cut(s) 408, 456
TasI AATT 1 cut(s) 52
TatI WGTACW 1 cut(s) 294
Tru1I TTAA 1 cut(s) 174
Tru9I TTAA 1 cut(s) 174
TscAI CASTG 1 cut(s) 325
TseI GCWGC 2 cut(s) 206, 434
TspDTI ATGAA 1 cut(s) 228
TspRI CASTG 1 cut(s) 325
VpaK11BI GGWCC 2 cut(s) 5, 343
XceI RCATGY 4 cut(s) 173, 243, 270, 340
XmiI GTMKAC 1 cut(s) 456
XspI CTAG 2 cut(s) 200, 478
ZraI GACGTC 1 cut(s) 369
ZrmI AGTACT 1 cut(s) 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.