Prupe.3G035900_v2.0.a1

2Fe-2S iron-sulfur cluster binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0009702
Physical Location & Seq
Forward (+)
2597337 .. 2600635
3299 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G035900.1

Sequence Viewer

Length: 537 bp
ATGTTTATTTCTAGAGTATCCCGGTTCGGAACTCAGATGGTCAAGCGTCTCACAAAAGGTACATTGACGTCTGCACCAAAGACAGGAAACTTACCTAAAGTTTACAATTATTTTTTCCATTCTCTGAAGCATCAAACTTTCTCTACAACAACTGCTCATGAGGAAGGGGATAAAGAAGTTGAAGAAATTTCTGTGACGTTTGTTGACAAGGATGAAATGGAGACGCATATTAAGGTTCCCATTGGCATGTCCATGCTAGAAGCTGCTCATGAACATGACATAGAACTTGAAGGAGCATGTGAAGGATCCTGCGCTTGTTCAACATGTCATGTGATTGTGAGAGATATGGAGTACTACAATAAACTCGAAGATCCAACTGATGAGGAAAATGACATGCTGGACCTCGCTTTCGGGCTCACAGAGACGTCTCGACTGGGTTGTCAAATGACTGCAAAACCTGAACTTGATGGAATATGCCTTGAACTGCCCAGTGCAACACGAAACTTTGCTGTTGATAGGTATGCCAAGGCCACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer2

Protein Analysis

179

Amino Acids

19.97

Weight (kDa)

4.97

Isoelectric Point (pI)

38.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 438
AatII GACGTC 2 cut(s) 71, 428
AclWI GGATC 3 cut(s) 300, 313, 365
AcsI RAATTY 1 cut(s) 186
AcuI CTGAAG 1 cut(s) 146
AcyI GRCGYC 2 cut(s) 68, 425
AfaI GTAC 2 cut(s) 61, 353
AfiI CCNNNNNNNGG 1 cut(s) 83
AflIII ACRYGT 1 cut(s) 323
AgsI TTSAA 4 cut(s) 182, 290, 321, 482
AluBI AGCT 1 cut(s) 263
AluI AGCT 1 cut(s) 263
Alw26I GTCTC 4 cut(s) 53, 215, 416, 432
AlwI GGATC 3 cut(s) 300, 313, 365
AoxI GGCC 1 cut(s) 528
ApeKI GCWGC 1 cut(s) 263
ApoI RAATTY 1 cut(s) 186
ArsI GACNNNNNNTTYG 2 cut(s) 392, 424
AspLEI GCGC 1 cut(s) 314
AspS9I GGNCC 1 cut(s) 400
AsuC2I CCSGG 1 cut(s) 22
AvaII GGWCC 1 cut(s) 400
BamHI GGATCC 1 cut(s) 305
BanII GRGCYC 1 cut(s) 417
BbvI GCAGC 1 cut(s) 250
BccI CCATC 2 cut(s) 31, 461
BciVI GTATCC 1 cut(s) 28
BcnI CCSGG 1 cut(s) 22
BcoDI GTCTC 4 cut(s) 53, 215, 416, 432
BfaI CTAG 2 cut(s) 12, 257
BfuI GTATCC 1 cut(s) 28
BisI GCNGC 1 cut(s) 264
BlsI GCNGC 1 cut(s) 265
BmcAI AGTACT 1 cut(s) 353
Bme1390I CCNGG 1 cut(s) 22
Bme18I GGWCC 1 cut(s) 400
BmgT120I GGNCC 1 cut(s) 400
BmiI GGNNCC 2 cut(s) 237, 307
BmrFI CCNGG 1 cut(s) 22
BmrI ACTGGG 2 cut(s) 443, 483
BmsI GCATC 1 cut(s) 139
BmuI ACTGGG 2 cut(s) 443, 483
BpuMI CCSGG 1 cut(s) 22
BsaHI GRCGYC 2 cut(s) 68, 425
BsaJI CCNNGG 1 cut(s) 525
Bsc4I CCNNNNNNNGG 1 cut(s) 83
Bse1I ACTGG 2 cut(s) 438, 489
BseDI CCNNGG 1 cut(s) 525
BseGI GGATG 1 cut(s) 217
BseLI CCNNNNNNNGG 1 cut(s) 83
BseMII CTCAG 1 cut(s) 47
BseNI ACTGG 2 cut(s) 438, 489
BseXI GCAGC 1 cut(s) 250
BsgI GTGCAG 1 cut(s) 57
BshFI GGCC 1 cut(s) 530
BsiSI CCGG 1 cut(s) 22
BslI CCNNNNNNNGG 1 cut(s) 83
BsmAI GTCTC 4 cut(s) 53, 215, 416, 432
BsmBI CGTCTC 4 cut(s) 53, 215, 416, 432
BsnI GGCC 1 cut(s) 530
Bsp1286I GDGCHC 1 cut(s) 417
Bsp143I GATC 2 cut(s) 305, 370
BspANI GGCC 1 cut(s) 530
BspCNI CTCAG 1 cut(s) 46
BspHI TCATGA 2 cut(s) 157, 268
BspLI GGNNCC 2 cut(s) 237, 307
BspPI GGATC 3 cut(s) 300, 313, 365
BsrI ACTGG 2 cut(s) 438, 489
BssECI CCNNGG 1 cut(s) 525
BssMI GATC 2 cut(s) 305, 370
BssNI GRCGYC 2 cut(s) 68, 425
BssT1I CCWWGG 1 cut(s) 525
BstACI GRCGYC 2 cut(s) 68, 425
BstDEI CTNAG 1 cut(s) 33
BstF5I GGATG 1 cut(s) 217
BstHHI GCGC 1 cut(s) 314
BstKTI GATC 2 cut(s) 308, 373
BstMAI GTCTC 4 cut(s) 53, 215, 416, 432
BstMBI GATC 2 cut(s) 305, 370
BstNSI RCATGY 4 cut(s) 250, 300, 327, 397
BstSCI CCNGG 1 cut(s) 20
BstV1I GCAGC 1 cut(s) 250
BstX2I RGATCY 2 cut(s) 305, 370
BstYI RGATCY 2 cut(s) 305, 370
BsuI GTATCC 1 cut(s) 28
BsuRI GGCC 1 cut(s) 530
BtsCI GGATG 1 cut(s) 217
BtsIMutI CAGTG 1 cut(s) 496
CciI TCATGA 2 cut(s) 157, 268
CfoI GCGC 1 cut(s) 314
Cfr13I GGNCC 1 cut(s) 400
CseI GACGC 2 cut(s) 35, 232
Csp6I GTAC 2 cut(s) 60, 352
CviAII CATG 9 cut(s) 158, 247, 253, 269, 275, 297, 324, 329, 394
CviJI RGCY 3 cut(s) 263, 415, 530
CviKI_1 RGCY 3 cut(s) 263, 415, 530
CviQI GTAC 2 cut(s) 60, 352
DdeI CTNAG 1 cut(s) 33
DpnI GATC 2 cut(s) 307, 372
DpnII GATC 2 cut(s) 305, 370
DrdI GACNNNNNNGTC 1 cut(s) 438
DseDI GACNNNNNNGTC 1 cut(s) 438
Eco130I CCWWGG 1 cut(s) 525
Eco24I GRGCYC 1 cut(s) 417
Eco47I GGWCC 1 cut(s) 400
Eco57I CTGAAG 1 cut(s) 146
EcoT14I CCWWGG 1 cut(s) 525
EcoT38I GRGCYC 1 cut(s) 417
ErhI CCWWGG 1 cut(s) 525
Esp3I CGTCTC 4 cut(s) 53, 215, 416, 432
FaeI CATG 9 cut(s) 161, 250, 256, 272, 278, 300, 327, 332, 397
FatI CATG 9 cut(s) 157, 246, 252, 268, 274, 296, 323, 328, 393
Fnu4HI GCNGC 1 cut(s) 264
FokI GGATG 1 cut(s) 224
FriOI GRGCYC 1 cut(s) 417
Fsp4HI GCNGC 1 cut(s) 264
FspBI CTAG 2 cut(s) 12, 257
GlaI GCGC 1 cut(s) 313
GluI GCNGC 1 cut(s) 264
HaeIII GGCC 1 cut(s) 530
HapII CCGG 1 cut(s) 22
HgaI GACGC 2 cut(s) 35, 232
HhaI GCGC 1 cut(s) 314
Hin1I GRCGYC 2 cut(s) 68, 425
Hin1II CATG 9 cut(s) 161, 250, 256, 272, 278, 300, 327, 332, 397
Hin6I GCGC 1 cut(s) 312
HinP1I GCGC 1 cut(s) 312
HincII GTYRAC 1 cut(s) 205
HindII GTYRAC 1 cut(s) 205
HpaII CCGG 1 cut(s) 22
Hpy166II GTNNAC 2 cut(s) 103, 205
Hpy188I TCNGA 3 cut(s) 29, 36, 126
Hpy188III TCNNGA 4 cut(s) 12, 158, 269, 429
Hpy8I GTNNAC 2 cut(s) 103, 205
HpyAV CCTTC 3 cut(s) 158, 284, 296
HpyCH4IV ACGT 3 cut(s) 68, 197, 425
HpyCH4V TGCA 3 cut(s) 74, 452, 494
HpyF3I CTNAG 1 cut(s) 33
HpySE526I ACGT 3 cut(s) 68, 197, 425
Hsp92I GRCGYC 2 cut(s) 68, 425
Hsp92II CATG 9 cut(s) 161, 250, 256, 272, 278, 300, 327, 332, 397
HspAI GCGC 1 cut(s) 312
Kzo9I GATC 2 cut(s) 305, 370
LmnI GCTCC 1 cut(s) 293
LpnPI CCDG 7 cut(s) 35, 69, 322, 383, 419, 471, 502
Lsp1109I GCAGC 1 cut(s) 250
LweI GCATC 1 cut(s) 139
MaeI CTAG 2 cut(s) 12, 257
MaeII ACGT 3 cut(s) 68, 197, 425
MaeIII GTNAC 1 cut(s) 193
MalI GATC 2 cut(s) 307, 372
MboI GATC 2 cut(s) 305, 370
MboII GAAGA 2 cut(s) 194, 380
MflI RGATCY 2 cut(s) 305, 370
MhlI GDGCHC 1 cut(s) 417
MluCI AATT 2 cut(s) 106, 186
MmeI TCCRAC 1 cut(s) 398
MnlI CCTC 3 cut(s) 154, 376, 413
MseI TTAA 1 cut(s) 231
MslI CAYNNNNRTG 3 cut(s) 245, 251, 273
MspI CCGG 1 cut(s) 22
MspR9I CCNGG 1 cut(s) 22
NciI CCSGG 1 cut(s) 22
NdeII GATC 2 cut(s) 305, 370
NlaIII CATG 9 cut(s) 161, 250, 256, 272, 278, 300, 327, 332, 397
NlaIV GGNNCC 2 cut(s) 237, 307
NmuCI GTSAC 1 cut(s) 193
NspI RCATGY 4 cut(s) 250, 300, 327, 397
PagI TCATGA 2 cut(s) 157, 268
PciI ACATGT 1 cut(s) 323
PkrI GCNGC 1 cut(s) 265
PscI ACATGT 1 cut(s) 323
PspN4I GGNNCC 2 cut(s) 237, 307
PspPI GGNCC 1 cut(s) 400
PsuI RGATCY 2 cut(s) 305, 370
RsaI GTAC 2 cut(s) 61, 353
RsaNI GTAC 2 cut(s) 60, 352
RseI CAYNNNNRTG 3 cut(s) 245, 251, 273
SaqAI TTAA 1 cut(s) 231
SatI GCNGC 1 cut(s) 264
Sau3AI GATC 2 cut(s) 305, 370
Sau96I GGNCC 1 cut(s) 400
ScaI AGTACT 1 cut(s) 353
ScrFI CCNGG 1 cut(s) 22
SduI GDGCHC 1 cut(s) 417
SfaNI GCATC 1 cut(s) 139
SinI GGWCC 1 cut(s) 400
SmiMI CAYNNNNRTG 3 cut(s) 245, 251, 273
Sse9I AATT 2 cut(s) 106, 186
SspMI CTAG 2 cut(s) 12, 257
StyD4I CCNGG 1 cut(s) 20
StyI CCWWGG 1 cut(s) 525
TaiI ACGT 3 cut(s) 71, 200, 428
TaqI TCGA 2 cut(s) 366, 430
TasI AATT 2 cut(s) 106, 186
TatI WGTACW 1 cut(s) 351
Tru1I TTAA 1 cut(s) 231
Tru9I TTAA 1 cut(s) 231
TscAI CASTG 1 cut(s) 496
TseFI GTSAC 1 cut(s) 193
TseI GCWGC 1 cut(s) 263
Tsp45I GTSAC 1 cut(s) 193
TspDTI ATGAA 2 cut(s) 228, 285
TspRI CASTG 1 cut(s) 496
VpaK11BI GGWCC 1 cut(s) 400
XapI RAATTY 1 cut(s) 186
XbaI TCTAGA 1 cut(s) 11
XceI RCATGY 4 cut(s) 250, 300, 327, 397
XspI CTAG 2 cut(s) 12, 257
ZraI GACGTC 2 cut(s) 69, 426
ZrmI AGTACT 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.