MD17G1150000.v1.1

2Fe-2S iron-sulfur cluster binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0009702
Physical Location & Seq
Reverse (-)
13734331 .. 13735342
1012 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1150000.v1.1.491

Sequence Viewer

Length: 336 bp
ATGTGCAGAATTTCTGTCACATTTGTGGACAAGGATGAGATGGCGAAGCATATTAAGATTCCCTTTGAAATGTCCATACTAGAAGCTGCTCATGAAAATGACATAGAACTTGAAGGAGGATGTGAAGGATCGTGCGCTTGCTCAACATGTCATGTGATTGTGAGAATCCAAACTGATGAGGAAAACGACATGCTGGATCTTGCTTTCGGGCTCACAGAGACCAGGTCCCGTCTGGGTTGTCAAATGGTTGCAAAACCCGAACTTGACGGAATATGCCTTGAACTGCCGAGTGCAACGCGCAACTTTGCTGTTGATAGGTATGCCAAGGCCACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer2

Protein Analysis

112

Amino Acids

12.3

Weight (kDa)

4.67

Isoelectric Point (pI)

46.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fer2 PF00111 22 - 85 1.7e-09 2Fe-2S iron-sulfur cluster binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 298
AclWI GGATC 2 cut(s) 136, 204
AcsI RAATTY 1 cut(s) 9
AflIII ACRYGT 1 cut(s) 146
AgsI TTSAA 3 cut(s) 68, 113, 281
AjnI CCWGG 1 cut(s) 221
AleI CACNNNNGTG 1 cut(s) 23
AluBI AGCT 1 cut(s) 86
AluI AGCT 1 cut(s) 86
Alw26I GTCTC 1 cut(s) 212
AlwI GGATC 2 cut(s) 136, 204
AoxI GGCC 1 cut(s) 327
ApeKI GCWGC 1 cut(s) 86
ApoI RAATTY 1 cut(s) 9
AspLEI GCGC 2 cut(s) 137, 300
AspS9I GGNCC 1 cut(s) 225
AvaII GGWCC 1 cut(s) 225
BanII GRGCYC 1 cut(s) 213
BbvI GCAGC 1 cut(s) 73
BccI CCATC 1 cut(s) 34
BciT130I CCWGG 1 cut(s) 223
BcoDI GTCTC 1 cut(s) 212
BfaI CTAG 1 cut(s) 80
BisI GCNGC 1 cut(s) 87
BlsI GCNGC 1 cut(s) 88
Bme1390I CCNGG 1 cut(s) 223
Bme18I GGWCC 1 cut(s) 225
BmgT120I GGNCC 1 cut(s) 225
BmiI GGNNCC 1 cut(s) 227
BmrFI CCNGG 1 cut(s) 223
BsaI GGTCTC 1 cut(s) 212
BsaJI CCNNGG 1 cut(s) 324
BseBI CCWGG 1 cut(s) 223
BseDI CCNNGG 1 cut(s) 324
BseGI GGATG 2 cut(s) 40, 125
BseXI GCAGC 1 cut(s) 73
BsgI GTGCAG 1 cut(s) 25
Bsh1236I CGCG 1 cut(s) 298
BshFI GGCC 1 cut(s) 329
BslFI GGGAC 1 cut(s) 211
BsmAI GTCTC 1 cut(s) 212
BsmFI GGGAC 1 cut(s) 211
BsnI GGCC 1 cut(s) 329
Bso31I GGTCTC 1 cut(s) 212
Bsp1286I GDGCHC 1 cut(s) 213
Bsp143I GATC 2 cut(s) 128, 196
BspANI GGCC 1 cut(s) 329
BspFNI CGCG 1 cut(s) 298
BspHI TCATGA 1 cut(s) 91
BspLI GGNNCC 1 cut(s) 227
BspPI GGATC 2 cut(s) 136, 204
BspTNI GGTCTC 1 cut(s) 212
BssECI CCNNGG 1 cut(s) 324
BssMI GATC 2 cut(s) 128, 196
BssT1I CCWWGG 1 cut(s) 324
Bst2UI CCWGG 1 cut(s) 223
BstC8I GCNNGC 1 cut(s) 139
BstF5I GGATG 2 cut(s) 40, 125
BstFNI CGCG 1 cut(s) 298
BstHHI GCGC 2 cut(s) 137, 300
BstKTI GATC 2 cut(s) 131, 199
BstMAI GTCTC 1 cut(s) 212
BstMBI GATC 2 cut(s) 128, 196
BstNI CCWGG 1 cut(s) 223
BstNSI RCATGY 2 cut(s) 150, 193
BstSCI CCNGG 1 cut(s) 221
BstUI CGCG 1 cut(s) 298
BstV1I GCAGC 1 cut(s) 73
BstX2I RGATCY 1 cut(s) 196
BstYI RGATCY 1 cut(s) 196
BsuRI GGCC 1 cut(s) 329
BtsCI GGATG 2 cut(s) 40, 125
Cac8I GCNNGC 1 cut(s) 139
CciI TCATGA 1 cut(s) 91
CfoI GCGC 2 cut(s) 137, 300
Cfr13I GGNCC 1 cut(s) 225
CsiI ACCWGGT 1 cut(s) 221
CviAII CATG 4 cut(s) 92, 147, 152, 190
CviJI RGCY 3 cut(s) 86, 211, 329
CviKI_1 RGCY 3 cut(s) 86, 211, 329
DpnI GATC 2 cut(s) 130, 198
DpnII GATC 2 cut(s) 128, 196
Eco130I CCWWGG 1 cut(s) 324
Eco24I GRGCYC 1 cut(s) 213
Eco31I GGTCTC 1 cut(s) 212
Eco47I GGWCC 1 cut(s) 225
EcoO109I RGGNCCY 1 cut(s) 225
EcoRII CCWGG 1 cut(s) 221
EcoT14I CCWWGG 1 cut(s) 324
EcoT38I GRGCYC 1 cut(s) 213
ErhI CCWWGG 1 cut(s) 324
FaeI CATG 4 cut(s) 95, 150, 155, 193
FalI AAGNNNNNCTT 2 cut(s) 47, 79
FaqI GGGAC 1 cut(s) 211
FatI CATG 4 cut(s) 91, 146, 151, 189
Fnu4HI GCNGC 1 cut(s) 87
FokI GGATG 2 cut(s) 47, 132
FriOI GRGCYC 1 cut(s) 213
Fsp4HI GCNGC 1 cut(s) 87
FspBI CTAG 1 cut(s) 80
GlaI GCGC 2 cut(s) 136, 299
GluI GCNGC 1 cut(s) 87
HaeIII GGCC 1 cut(s) 329
HhaI GCGC 2 cut(s) 137, 300
Hin1II CATG 4 cut(s) 95, 150, 155, 193
Hin6I GCGC 2 cut(s) 135, 298
HinP1I GCGC 2 cut(s) 135, 298
HinfI GANTC 2 cut(s) 58, 165
Hpy166II GTNNAC 1 cut(s) 28
Hpy188III TCNNGA 1 cut(s) 92
Hpy8I GTNNAC 1 cut(s) 28
HpyAV CCTTC 2 cut(s) 107, 119
HpyCH4V TGCA 3 cut(s) 6, 251, 293
Hsp92II CATG 4 cut(s) 95, 150, 155, 193
HspAI GCGC 2 cut(s) 135, 298
Kzo9I GATC 2 cut(s) 128, 196
LpnPI CCDG 4 cut(s) 179, 208, 218, 235
Lsp1109I GCAGC 1 cut(s) 73
MabI ACCWGGT 1 cut(s) 221
MaeI CTAG 1 cut(s) 80
MaeIII GTNAC 1 cut(s) 16
MalI GATC 2 cut(s) 130, 198
MboI GATC 2 cut(s) 128, 196
MflI RGATCY 1 cut(s) 196
MhlI GDGCHC 1 cut(s) 213
MluCI AATT 1 cut(s) 9
MnlI CCTC 2 cut(s) 110, 172
MseI TTAA 1 cut(s) 54
MslI CAYNNNNRTG 2 cut(s) 23, 96
MspR9I CCNGG 1 cut(s) 223
MvaI CCWGG 1 cut(s) 223
MvnI CGCG 1 cut(s) 298
NdeII GATC 2 cut(s) 128, 196
NlaIII CATG 4 cut(s) 95, 150, 155, 193
NlaIV GGNNCC 1 cut(s) 227
NmeAIII GCCGAG 1 cut(s) 312
NmuCI GTSAC 1 cut(s) 16
NspI RCATGY 2 cut(s) 150, 193
OliI CACNNNNGTG 1 cut(s) 23
PagI TCATGA 1 cut(s) 91
PciI ACATGT 1 cut(s) 146
PfeI GAWTC 2 cut(s) 58, 165
PflFI GACNNNGTC 1 cut(s) 223
PkrI GCNGC 1 cut(s) 88
PpuMI RGGWCCY 1 cut(s) 225
PscI ACATGT 1 cut(s) 146
Psp5II RGGWCCY 1 cut(s) 225
Psp6I CCWGG 1 cut(s) 221
PspGI CCWGG 1 cut(s) 221
PspN4I GGNNCC 1 cut(s) 227
PspPI GGNCC 1 cut(s) 225
PspPPI RGGWCCY 1 cut(s) 225
PsuI RGATCY 1 cut(s) 196
PsyI GACNNNGTC 1 cut(s) 223
RseI CAYNNNNRTG 2 cut(s) 23, 96
SaqAI TTAA 1 cut(s) 54
SatI GCNGC 1 cut(s) 87
Sau3AI GATC 2 cut(s) 128, 196
Sau96I GGNCC 1 cut(s) 225
ScrFI CCNGG 1 cut(s) 223
SduI GDGCHC 1 cut(s) 213
SetI ASST 3 cut(s) 88, 227, 320
SexAI ACCWGGT 1 cut(s) 221
SinI GGWCC 1 cut(s) 225
SmiMI CAYNNNNRTG 2 cut(s) 23, 96
Sse9I AATT 1 cut(s) 9
SspMI CTAG 1 cut(s) 80
StyD4I CCNGG 1 cut(s) 221
StyI CCWWGG 1 cut(s) 324
TasI AATT 1 cut(s) 9
TfiI GAWTC 2 cut(s) 58, 165
Tru1I TTAA 1 cut(s) 54
Tru9I TTAA 1 cut(s) 54
TseFI GTSAC 1 cut(s) 16
TseI GCWGC 1 cut(s) 86
Tsp45I GTSAC 1 cut(s) 16
TspDTI ATGAA 1 cut(s) 108
TspGWI ACGGA 1 cut(s) 282
Tth111I GACNNNGTC 1 cut(s) 223
VpaK11BI GGWCC 1 cut(s) 225
XapI RAATTY 1 cut(s) 9
XceI RCATGY 2 cut(s) 150, 193
XcmI CCANNNNNNNNNTGG 1 cut(s) 229
XspI CTAG 1 cut(s) 80
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.