Rroxscaffold_2G00099950

2Fe-2S iron-sulfur cluster binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0009702
Physical Location & Seq
Forward (+)
21716156 .. 21718855
2700 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00099950.1

Sequence Viewer

Length: 555 bp
ATGGTTGTTTCTAGATTTTCGGGCCTAGGCAGGCAGATGATCAAGTATCTCAGAAAAGGTCCATTGACATTTTCACCAAAAGCAGGAAACTTATGTGCAAGTTACAATTATTTTTCCCATACTTTGACGCCTCGTACTTTTTCTACAACAACTGCTCATGAGGAAGGGAACAGACAAATAGAAGAAATATCTGTGATATTTGTTGATAATGATGGGGAGAAGCATGTTAAAGTTCCTGTTGGAAAGTCTATACTAGAAGCTGCTCATGAAAATGATATTGAACTTGAAGGAGCATGTGAAGGATCCTGTGCTTGTTCTACATGCCATGTGATTGTGAGAGATTTGGATTACTACAATAAACTGGAAGATCCCACTGATGAGGAAAATGACATGCTGGACCTTGCATTCGGGCTCACAGAGACGTCTCGTCTGGGTTGTCAAGTGATTGCAACACCTGAACTCGATGGAATATGCCTTGAACTGCCCGCGGCCACTGGAACTTTGCTGTCGACAAGTATGCCAAAGCCACCTAGAATTCTTCAGATGGGGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer2

Protein Analysis

184

Amino Acids

20.18

Weight (kDa)

4.91

Isoelectric Point (pI)

45.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fer2 PF00111 69 - 151 1.3e-12 2Fe-2S iron-sulfur cluster binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 425
AccI GTMKAC 1 cut(s) 509
AccII CGCG 1 cut(s) 488
AciI CCGC 2 cut(s) 486, 488
AclWI GGATC 3 cut(s) 297, 310, 362
AcoI YGGCCR 1 cut(s) 489
AcsI RAATTY 1 cut(s) 534
AcuI CTGAAG 1 cut(s) 524
AcyI GRCGYC 2 cut(s) 128, 422
AfaI GTAC 1 cut(s) 136
AfiI CCNNNNNNNGG 1 cut(s) 83
AgsI TTSAA 3 cut(s) 281, 287, 479
AhdI GACNNNNNGTC 1 cut(s) 426
AluBI AGCT 1 cut(s) 260
AluI AGCT 1 cut(s) 260
Alw26I GTCTC 2 cut(s) 413, 429
AlwI GGATC 3 cut(s) 297, 310, 362
AoxI GGCC 2 cut(s) 22, 489
ApeKI GCWGC 1 cut(s) 260
ApoI RAATTY 1 cut(s) 534
ArsI GACNNNNNNTTYG 2 cut(s) 389, 421
AspA2I CCTAGG 1 cut(s) 25
AspS9I GGNCC 3 cut(s) 22, 59, 397
AsuHPI GGTGA 1 cut(s) 66
AvaII GGWCC 2 cut(s) 59, 397
AvrII CCTAGG 1 cut(s) 25
BamHI GGATCC 1 cut(s) 302
BanII GRGCYC 1 cut(s) 414
BbvI GCAGC 1 cut(s) 247
BccI CCATC 3 cut(s) 206, 458, 538
BcgI CGANNNNNNTGC 2 cut(s) 499, 533
BclI TGATCA 1 cut(s) 39
BcoDI GTCTC 2 cut(s) 413, 429
BfaI CTAG 4 cut(s) 12, 26, 254, 531
BisI GCNGC 2 cut(s) 261, 489
BlnI CCTAGG 1 cut(s) 25
BlsI GCNGC 2 cut(s) 262, 490
Bme18I GGWCC 2 cut(s) 59, 397
BmeRI GACNNNNNGTC 1 cut(s) 426
BmgT120I GGNCC 3 cut(s) 22, 59, 397
BmiI GGNNCC 1 cut(s) 304
BsaHI GRCGYC 2 cut(s) 128, 422
BsaJI CCNNGG 2 cut(s) 25, 486
Bsc4I CCNNNNNNNGG 1 cut(s) 83
Bse1I ACTGG 2 cut(s) 366, 499
BseDI CCNNGG 2 cut(s) 25, 486
BseLI CCNNNNNNNGG 1 cut(s) 83
BseMII CTCAG 1 cut(s) 64
BseNI ACTGG 2 cut(s) 366, 499
BseXI GCAGC 1 cut(s) 247
Bsh1236I CGCG 1 cut(s) 488
BshFI GGCC 2 cut(s) 24, 491
BslI CCNNNNNNNGG 1 cut(s) 83
BsmAI GTCTC 2 cut(s) 413, 429
BsmBI CGTCTC 2 cut(s) 413, 429
BsmI GAATGC 1 cut(s) 404
BsnI GGCC 2 cut(s) 24, 491
Bsp1286I GDGCHC 1 cut(s) 414
Bsp143I GATC 3 cut(s) 39, 302, 367
BspACI CCGC 2 cut(s) 486, 488
BspANI GGCC 2 cut(s) 24, 491
BspCNI CTCAG 1 cut(s) 63
BspFNI CGCG 1 cut(s) 488
BspHI TCATGA 2 cut(s) 157, 265
BspLI GGNNCC 1 cut(s) 304
BspPI GGATC 3 cut(s) 297, 310, 362
BsrI ACTGG 2 cut(s) 366, 499
BssECI CCNNGG 2 cut(s) 25, 486
BssMI GATC 3 cut(s) 39, 302, 367
BssNI GRCGYC 2 cut(s) 128, 422
BssT1I CCWWGG 1 cut(s) 25
BstACI GRCGYC 2 cut(s) 128, 422
BstC8I GCNNGC 2 cut(s) 32, 486
BstDEI CTNAG 1 cut(s) 50
BstDSI CCRYGG 1 cut(s) 486
BstFNI CGCG 1 cut(s) 488
BstKTI GATC 3 cut(s) 42, 305, 370
BstMAI GTCTC 2 cut(s) 413, 429
BstMBI GATC 3 cut(s) 39, 302, 367
BstNSI RCATGY 4 cut(s) 227, 297, 324, 394
BstUI CGCG 1 cut(s) 488
BstV1I GCAGC 1 cut(s) 247
BstX2I RGATCY 2 cut(s) 302, 367
BstYI RGATCY 2 cut(s) 302, 367
BsuRI GGCC 2 cut(s) 24, 491
BtgI CCRYGG 1 cut(s) 486
BtsIMutI CAGTG 2 cut(s) 372, 492
Cac8I GCNNGC 2 cut(s) 32, 486
CciI TCATGA 2 cut(s) 157, 265
Cfr13I GGNCC 3 cut(s) 22, 59, 397
Cfr42I CCGCGG 1 cut(s) 489
CseI GACGC 1 cut(s) 136
Csp6I GTAC 1 cut(s) 135
CviAII CATG 7 cut(s) 158, 224, 266, 294, 321, 326, 391
CviJI RGCY 5 cut(s) 24, 260, 412, 491, 526
CviKI_1 RGCY 5 cut(s) 24, 260, 412, 491, 526
CviQI GTAC 1 cut(s) 135
DdeI CTNAG 1 cut(s) 50
DpnI GATC 3 cut(s) 41, 304, 369
DpnII GATC 3 cut(s) 39, 302, 367
DriI GACNNNNNGTC 1 cut(s) 426
EaeI YGGCCR 1 cut(s) 489
Eam1105I GACNNNNNGTC 1 cut(s) 426
Eco130I CCWWGG 1 cut(s) 25
Eco24I GRGCYC 1 cut(s) 414
Eco47I GGWCC 2 cut(s) 59, 397
Eco57I CTGAAG 1 cut(s) 524
EcoRI GAATTC 1 cut(s) 534
EcoT14I CCWWGG 1 cut(s) 25
EcoT38I GRGCYC 1 cut(s) 414
ErhI CCWWGG 1 cut(s) 25
Esp3I CGTCTC 2 cut(s) 413, 429
FaeI CATG 7 cut(s) 161, 227, 269, 297, 324, 329, 394
FatI CATG 7 cut(s) 157, 223, 265, 293, 320, 325, 390
FauI CCCGC 1 cut(s) 493
FbaI TGATCA 1 cut(s) 39
FblI GTMKAC 1 cut(s) 509
Fnu4HI GCNGC 2 cut(s) 261, 489
FriOI GRGCYC 1 cut(s) 414
Fsp4HI GCNGC 2 cut(s) 261, 489
FspBI CTAG 4 cut(s) 12, 26, 254, 531
GluI GCNGC 2 cut(s) 261, 489
HaeIII GGCC 2 cut(s) 24, 491
HgaI GACGC 1 cut(s) 136
Hin1I GRCGYC 2 cut(s) 128, 422
Hin1II CATG 7 cut(s) 161, 227, 269, 297, 324, 329, 394
HincII GTYRAC 1 cut(s) 510
HindII GTYRAC 1 cut(s) 510
HphI GGTGA 1 cut(s) 66
Hpy166II GTNNAC 1 cut(s) 510
Hpy188I TCNGA 2 cut(s) 53, 543
Hpy188III TCNNGA 3 cut(s) 12, 158, 266
Hpy8I GTNNAC 1 cut(s) 510
HpyAV CCTTC 3 cut(s) 158, 281, 293
HpyCH4IV ACGT 1 cut(s) 422
HpyCH4V TGCA 3 cut(s) 98, 404, 449
HpyF3I CTNAG 1 cut(s) 50
HpySE526I ACGT 1 cut(s) 422
Hsp92I GRCGYC 2 cut(s) 128, 422
Hsp92II CATG 7 cut(s) 161, 227, 269, 297, 324, 329, 394
Ksp22I TGATCA 1 cut(s) 39
KspI CCGCGG 1 cut(s) 489
Kzo9I GATC 3 cut(s) 39, 302, 367
LmnI GCTCC 1 cut(s) 290
LpnPI CCDG 9 cut(s) 16, 69, 249, 319, 347, 380, 416, 468, 480
Lsp1109I GCAGC 1 cut(s) 247
MaeI CTAG 4 cut(s) 12, 26, 254, 531
MaeII ACGT 1 cut(s) 422
MaeIII GTNAC 1 cut(s) 101
MalI GATC 3 cut(s) 41, 304, 369
MboI GATC 3 cut(s) 39, 302, 367
MboII GAAGA 3 cut(s) 194, 377, 530
MflI RGATCY 2 cut(s) 302, 367
MhlI GDGCHC 1 cut(s) 414
MluCI AATT 2 cut(s) 106, 534
MmeI TCCRAC 1 cut(s) 220
MnlI CCTC 3 cut(s) 141, 154, 373
MseI TTAA 1 cut(s) 228
MslI CAYNNNNRTG 1 cut(s) 270
MspA1I CMGCKG 1 cut(s) 488
Mva1269I GAATGC 1 cut(s) 404
MvnI CGCG 1 cut(s) 488
NdeII GATC 3 cut(s) 39, 302, 367
NlaIII CATG 7 cut(s) 161, 227, 269, 297, 324, 329, 394
NlaIV GGNNCC 1 cut(s) 304
NspI RCATGY 4 cut(s) 227, 297, 324, 394
PagI TCATGA 2 cut(s) 157, 265
PctI GAATGC 1 cut(s) 404
PkrI GCNGC 2 cut(s) 262, 490
PspN4I GGNNCC 1 cut(s) 304
PspPI GGNCC 3 cut(s) 22, 59, 397
PsuI RGATCY 2 cut(s) 302, 367
RsaI GTAC 1 cut(s) 136
RsaNI GTAC 1 cut(s) 135
RseI CAYNNNNRTG 1 cut(s) 270
SacII CCGCGG 1 cut(s) 489
SalI GTCGAC 1 cut(s) 508
SaqAI TTAA 1 cut(s) 228
SatI GCNGC 2 cut(s) 261, 489
Sau3AI GATC 3 cut(s) 39, 302, 367
Sau96I GGNCC 3 cut(s) 22, 59, 397
SduI GDGCHC 1 cut(s) 414
SetI ASST 6 cut(s) 61, 262, 402, 425, 457, 532
Sfr303I CCGCGG 1 cut(s) 489
SgrBI CCGCGG 1 cut(s) 489
SinI GGWCC 2 cut(s) 59, 397
SmiMI CAYNNNNRTG 1 cut(s) 270
Sse9I AATT 2 cut(s) 106, 534
SsiI CCGC 2 cut(s) 486, 488
SspMI CTAG 4 cut(s) 12, 26, 254, 531
StyI CCWWGG 1 cut(s) 25
TaiI ACGT 1 cut(s) 425
TaqI TCGA 2 cut(s) 462, 509
TasI AATT 2 cut(s) 106, 534
TauI GCSGC 1 cut(s) 491
Tru1I TTAA 1 cut(s) 228
Tru9I TTAA 1 cut(s) 228
TscAI CASTG 2 cut(s) 379, 499
TseI GCWGC 1 cut(s) 260
TspDTI ATGAA 1 cut(s) 282
TspRI CASTG 2 cut(s) 379, 499
VpaK11BI GGWCC 2 cut(s) 59, 397
XapI RAATTY 1 cut(s) 534
XbaI TCTAGA 1 cut(s) 11
XceI RCATGY 4 cut(s) 227, 297, 324, 394
XmaJI CCTAGG 1 cut(s) 25
XmiI GTMKAC 1 cut(s) 509
XspI CTAG 4 cut(s) 12, 26, 254, 531
ZraI GACGTC 1 cut(s) 423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.