MD09G1176800.v1.1

2Fe-2S iron-sulfur cluster binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0009702
Physical Location & Seq
Forward (+)
15114042 .. 15120829
6788 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1176800.v1.1.491

Sequence Viewer

Length: 537 bp
ATGCTTGTTTCTAGACTAGCCGCTGTCAGAACTCAGATGATCAACCATGTAACAAAAGGTCCATTGAAGTCTTCACCAAAGACAGGAAACTTCGGTGGACTTTACAGTTATATTTTCCATACTCCGAAGCATCAAGCTTTCTCTACGACAACCCTGCATGAGGAGGGGGATAAAAAAGTAGAAGAAATTTCTGTCACGTTTGTTGACAAGGATGAGATGGAGAAGCATATTAAGGTTCCTGTTGGAATGTCCATGTTAGAAGCTGCTCATGAAAATGACATAGAACTTGAAGGAGCATGTGAAGGATCGTGTGCTTGTTCGACATGTCATGTGATTGTGAGAGATATGGAATACTACAATAAACTCGAAGATCCAACTGATGAGGAAAATGACATGCTGGATCTTGCTTTCGGGCTCACAGAGACGTCTCGTCTGGGTTGTCAAATGGTTGCAAAACGAGAACTTGACGGAATATGCCTTGAACTGCCGAGTGCAACGCGCAACTTTGCTGTTGATAGGCATGCCAAGGCCACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer2

Protein Analysis

179

Amino Acids

19.8

Weight (kDa)

5.23

Isoelectric Point (pI)

39.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fer2 PF00111 71 - 152 4e-12 2Fe-2S iron-sulfur cluster binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 428
AccII CGCG 1 cut(s) 499
AciI CCGC 1 cut(s) 21
AclWI GGATC 3 cut(s) 313, 365, 408
AcsI RAATTY 1 cut(s) 186
AcyI GRCGYC 1 cut(s) 425
AfiI CCNNNNNNNGG 2 cut(s) 83, 160
AflIII ACRYGT 1 cut(s) 323
AgsI TTSAA 3 cut(s) 67, 290, 482
AhdI GACNNNNNGTC 1 cut(s) 429
AluBI AGCT 2 cut(s) 137, 263
AluI AGCT 2 cut(s) 137, 263
Alw26I GTCTC 2 cut(s) 416, 432
AlwI GGATC 3 cut(s) 313, 365, 408
AoxI GGCC 1 cut(s) 528
ApeKI GCWGC 1 cut(s) 263
ApoI RAATTY 1 cut(s) 186
AspLEI GCGC 1 cut(s) 501
AspS9I GGNCC 1 cut(s) 59
AsuHPI GGTGA 1 cut(s) 66
AvaII GGWCC 1 cut(s) 59
BanII GRGCYC 1 cut(s) 417
BbsI GAAGAC 1 cut(s) 63
BbvI GCAGC 1 cut(s) 250
BccI CCATC 1 cut(s) 211
BcgI CGANNNNNNTGC 2 cut(s) 136, 170
BclI TGATCA 1 cut(s) 39
BcoDI GTCTC 2 cut(s) 416, 432
BfaI CTAG 2 cut(s) 12, 17
BisI GCNGC 2 cut(s) 21, 264
BlsI GCNGC 2 cut(s) 22, 265
Bme18I GGWCC 1 cut(s) 59
BmeRI GACNNNNNGTC 1 cut(s) 429
BmgT120I GGNCC 1 cut(s) 59
BmiI GGNNCC 1 cut(s) 237
BmsI GCATC 1 cut(s) 139
BpiI GAAGAC 1 cut(s) 63
BsaHI GRCGYC 1 cut(s) 425
BsaJI CCNNGG 1 cut(s) 525
Bsc4I CCNNNNNNNGG 2 cut(s) 83, 160
BseDI CCNNGG 1 cut(s) 525
BseGI GGATG 1 cut(s) 217
BseLI CCNNNNNNNGG 2 cut(s) 83, 160
BseMII CTCAG 1 cut(s) 47
BseRI GAGGAG 1 cut(s) 176
BseXI GCAGC 1 cut(s) 250
Bsh1236I CGCG 1 cut(s) 499
BshFI GGCC 1 cut(s) 530
BslI CCNNNNNNNGG 2 cut(s) 83, 160
BsmAI GTCTC 2 cut(s) 416, 432
BsmBI CGTCTC 2 cut(s) 416, 432
BsnI GGCC 1 cut(s) 530
Bsp1286I GDGCHC 1 cut(s) 417
Bsp143I GATC 4 cut(s) 39, 305, 370, 400
BspACI CCGC 1 cut(s) 21
BspANI GGCC 1 cut(s) 530
BspCNI CTCAG 1 cut(s) 46
BspFNI CGCG 1 cut(s) 499
BspHI TCATGA 1 cut(s) 268
BspLI GGNNCC 1 cut(s) 237
BspPI GGATC 3 cut(s) 313, 365, 408
BssECI CCNNGG 1 cut(s) 525
BssMI GATC 4 cut(s) 39, 305, 370, 400
BssNI GRCGYC 1 cut(s) 425
BssT1I CCWWGG 1 cut(s) 525
Bst4CI ACNGT 1 cut(s) 107
BstACI GRCGYC 1 cut(s) 425
BstC8I GCNNGC 1 cut(s) 522
BstDEI CTNAG 1 cut(s) 33
BstENI CCTNNNNNAGG 1 cut(s) 158
BstF5I GGATG 1 cut(s) 217
BstFNI CGCG 1 cut(s) 499
BstHHI GCGC 1 cut(s) 501
BstKTI GATC 4 cut(s) 42, 308, 373, 403
BstMAI GTCTC 2 cut(s) 416, 432
BstMBI GATC 4 cut(s) 39, 305, 370, 400
BstNSI RCATGY 4 cut(s) 300, 327, 397, 524
BstUI CGCG 1 cut(s) 499
BstV1I GCAGC 1 cut(s) 250
BstV2I GAAGAC 1 cut(s) 63
BstX2I RGATCY 2 cut(s) 370, 400
BstYI RGATCY 2 cut(s) 370, 400
BsuRI GGCC 1 cut(s) 530
BtsCI GGATG 1 cut(s) 217
Cac8I GCNNGC 1 cut(s) 522
CciI TCATGA 1 cut(s) 268
CfoI GCGC 1 cut(s) 501
Cfr13I GGNCC 1 cut(s) 59
CviAII CATG 9 cut(s) 47, 158, 253, 269, 297, 324, 329, 394, 521
CviJI RGCY 5 cut(s) 20, 137, 263, 415, 530
CviKI_1 RGCY 5 cut(s) 20, 137, 263, 415, 530
DdeI CTNAG 1 cut(s) 33
DpnI GATC 4 cut(s) 41, 307, 372, 402
DpnII GATC 4 cut(s) 39, 305, 370, 400
DriI GACNNNNNGTC 1 cut(s) 429
Eam1105I GACNNNNNGTC 1 cut(s) 429
Eco130I CCWWGG 1 cut(s) 525
Eco24I GRGCYC 1 cut(s) 417
Eco47I GGWCC 1 cut(s) 59
EcoNI CCTNNNNNAGG 1 cut(s) 158
EcoT14I CCWWGG 1 cut(s) 525
EcoT38I GRGCYC 1 cut(s) 417
ErhI CCWWGG 1 cut(s) 525
Esp3I CGTCTC 2 cut(s) 416, 432
FaeI CATG 9 cut(s) 50, 161, 256, 272, 300, 327, 332, 397, 524
FatI CATG 9 cut(s) 46, 157, 252, 268, 296, 323, 328, 393, 520
FbaI TGATCA 1 cut(s) 39
Fnu4HI GCNGC 2 cut(s) 21, 264
FokI GGATG 1 cut(s) 224
FriOI GRGCYC 1 cut(s) 417
Fsp4HI GCNGC 2 cut(s) 21, 264
FspBI CTAG 2 cut(s) 12, 17
GlaI GCGC 1 cut(s) 500
GluI GCNGC 2 cut(s) 21, 264
HaeIII GGCC 1 cut(s) 530
HhaI GCGC 1 cut(s) 501
Hin1I GRCGYC 1 cut(s) 425
Hin1II CATG 9 cut(s) 50, 161, 256, 272, 300, 327, 332, 397, 524
Hin6I GCGC 1 cut(s) 499
HinP1I GCGC 1 cut(s) 499
HincII GTYRAC 1 cut(s) 205
HindII GTYRAC 1 cut(s) 205
HindIII AAGCTT 1 cut(s) 135
HphI GGTGA 1 cut(s) 66
Hpy166II GTNNAC 2 cut(s) 98, 205
Hpy188I TCNGA 3 cut(s) 29, 36, 126
Hpy188III TCNNGA 2 cut(s) 12, 269
Hpy8I GTNNAC 2 cut(s) 98, 205
HpyAV CCTTC 2 cut(s) 284, 296
HpyCH4III ACNGT 1 cut(s) 107
HpyCH4IV ACGT 2 cut(s) 197, 425
HpyCH4V TGCA 3 cut(s) 157, 452, 494
HpyF3I CTNAG 1 cut(s) 33
HpySE526I ACGT 2 cut(s) 197, 425
Hsp92I GRCGYC 1 cut(s) 425
Hsp92II CATG 9 cut(s) 50, 161, 256, 272, 300, 327, 332, 397, 524
HspAI GCGC 1 cut(s) 499
Ksp22I TGATCA 1 cut(s) 39
Kzo9I GATC 4 cut(s) 39, 305, 370, 400
LmnI GCTCC 1 cut(s) 293
LpnPI CCDG 5 cut(s) 69, 167, 252, 383, 419
Lsp1109I GCAGC 1 cut(s) 250
LweI GCATC 1 cut(s) 139
MaeI CTAG 2 cut(s) 12, 17
MaeII ACGT 2 cut(s) 197, 425
MaeIII GTNAC 2 cut(s) 49, 193
MalI GATC 4 cut(s) 41, 307, 372, 402
MboI GATC 4 cut(s) 39, 305, 370, 400
MboII GAAGA 3 cut(s) 63, 194, 380
MflI RGATCY 2 cut(s) 370, 400
MhlI GDGCHC 1 cut(s) 417
MluCI AATT 1 cut(s) 186
MmeI TCCRAC 2 cut(s) 223, 398
MnlI CCTC 3 cut(s) 154, 157, 376
MseI TTAA 1 cut(s) 231
MslI CAYNNNNRTG 1 cut(s) 273
MspA1I CMGCKG 1 cut(s) 23
MvnI CGCG 1 cut(s) 499
NdeII GATC 4 cut(s) 39, 305, 370, 400
NlaIII CATG 9 cut(s) 50, 161, 256, 272, 300, 327, 332, 397, 524
NlaIV GGNNCC 1 cut(s) 237
NmeAIII GCCGAG 1 cut(s) 513
NmuCI GTSAC 1 cut(s) 193
NspI RCATGY 4 cut(s) 300, 327, 397, 524
PaeI GCATGC 1 cut(s) 524
PagI TCATGA 1 cut(s) 268
PciI ACATGT 1 cut(s) 323
PkrI GCNGC 2 cut(s) 22, 265
PscI ACATGT 1 cut(s) 323
PspN4I GGNNCC 1 cut(s) 237
PspPI GGNCC 1 cut(s) 59
PsuI RGATCY 2 cut(s) 370, 400
RseI CAYNNNNRTG 1 cut(s) 273
SaqAI TTAA 1 cut(s) 231
SatI GCNGC 2 cut(s) 21, 264
Sau3AI GATC 4 cut(s) 39, 305, 370, 400
Sau96I GGNCC 1 cut(s) 59
SduI GDGCHC 1 cut(s) 417
SetI ASST 6 cut(s) 61, 139, 200, 237, 265, 428
SfaNI GCATC 1 cut(s) 139
SinI GGWCC 1 cut(s) 59
SmiMI CAYNNNNRTG 1 cut(s) 273
SphI GCATGC 1 cut(s) 524
Sse9I AATT 1 cut(s) 186
SsiI CCGC 1 cut(s) 21
SspMI CTAG 2 cut(s) 12, 17
StyI CCWWGG 1 cut(s) 525
TaaI ACNGT 1 cut(s) 107
TaiI ACGT 2 cut(s) 200, 428
TaqI TCGA 2 cut(s) 320, 366
TasI AATT 1 cut(s) 186
TauI GCSGC 1 cut(s) 23
Tru1I TTAA 1 cut(s) 231
Tru9I TTAA 1 cut(s) 231
TseFI GTSAC 1 cut(s) 193
TseI GCWGC 1 cut(s) 263
Tsp45I GTSAC 1 cut(s) 193
TspDTI ATGAA 1 cut(s) 285
TspGWI ACGGA 1 cut(s) 483
VpaK11BI GGWCC 1 cut(s) 59
XagI CCTNNNNNAGG 1 cut(s) 158
XapI RAATTY 1 cut(s) 186
XbaI TCTAGA 1 cut(s) 11
XceI RCATGY 4 cut(s) 300, 327, 397, 524
XspI CTAG 2 cut(s) 12, 17
ZraI GACGTC 1 cut(s) 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.