FvH4_6g35350

DnaJ homolog subfamily B member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0010346
Physical Location & Seq
Reverse (-)
27909817 .. 27912856
3040 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g35350.t1

Sequence Viewer

Length: 714 bp
ATGGAAGAGAAAGGCAATGACTTTTATGCTGTTATGGGGTTGAAGAAGGAATGCTCTGACTCGGAGCTCAGGAATGCTTATAAGAAACTTGCACTGATATGGCACCCAGATCGTTGCTCTGCCTCAGGAAATTCAAAGTTCGTGGAAGAAGCCAAGAAGAAGTTTCAGGACATTCAACAAGCCTATTCTGTTCTGTCCGACGCCAACAAGAGGTTTCTGTACGATGTAGGAGCTTATGAAAGTGATGATGACGAAAATGGAATGGGTGATTTTTTAAACGAGATGGCGGTGATGATGAGCCAGACTAAGCCGAATGAAAATGGAGGAGAGAGCTTCGAACAATTGCAGGAGCTCTTTGAAGAAATGTTTCAGGGGGATATTGAGGGCTTTAGCTCCTGCTCTCAGCCTCCTACTTCCTGTTCTACTTCCTCATCTTCATACGCATTGTACTGTGAAAATTCTACTCCCAGTAACAAACGTAATTCCTCCGCAATGAATTATGGCAACGCAACCCTGGACAGTTCTGGTTTTGATGCTCATTTTCACAATTTCTGTGTAGGGGCGGGAAGCCAGCAAAGGATCGGGAAGGGGATGCCAGGAAGAGAAAGGATTCCAGGAGGAGTAACCGGTAGGCGAAGGGAGGGCAGGAAGCAAAAGGTTACATCCGGGCAAGATGTCTCCTCTAATGATCATTCGGGCATTTCAGCTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0051087
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

238

Amino Acids

26.13

Weight (kDa)

5.23

Isoelectric Point (pI)

53.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 7 - 75 8e-20 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 81
AccB1I GGYRCC 1 cut(s) 102
AciI CCGC 3 cut(s) 287, 489, 563
AclWI GGATC 1 cut(s) 587
AcsI RAATTY 2 cut(s) 130, 457
AcyI GRCGYC 1 cut(s) 201
AfaI GTAC 2 cut(s) 221, 449
AfiI CCNNNNNNNGG 1 cut(s) 210
AgeI ACCGGT 1 cut(s) 626
AgsI TTSAA 4 cut(s) 43, 135, 176, 359
AjnI CCWGG 3 cut(s) 513, 595, 613
AluBI AGCT 6 cut(s) 67, 233, 333, 352, 393, 707
AluI AGCT 6 cut(s) 67, 233, 333, 352, 393, 707
Alw21I GWGCWC 2 cut(s) 69, 354
Alw26I GTCTC 1 cut(s) 682
AlwI GGATC 1 cut(s) 587
ApoI RAATTY 2 cut(s) 130, 457
AsiGI ACCGGT 1 cut(s) 626
Asp700I GAANNNNTTC 2 cut(s) 366, 609
AsuC2I CCSGG 1 cut(s) 667
AsuHPI GGTGA 2 cut(s) 278, 301
AsuII TTCGAA 1 cut(s) 336
AxyI CCTNAGG 1 cut(s) 124
BanI GGYRCC 1 cut(s) 102
BanII GRGCYC 2 cut(s) 69, 354
Bbv12I GWGCWC 2 cut(s) 69, 354
BccI CCATC 1 cut(s) 277
BcgI CGANNNNNNTGC 2 cut(s) 92, 126
BciT130I CCWGG 3 cut(s) 515, 597, 615
BclI TGATCA 1 cut(s) 688
BcnI CCSGG 1 cut(s) 667
BcoDI GTCTC 1 cut(s) 682
BfaI CTAG 1 cut(s) 708
Bme1390I CCNGG 4 cut(s) 515, 597, 615, 667
BmiI GGNNCC 1 cut(s) 104
BmrFI CCNGG 4 cut(s) 515, 597, 615, 667
BmrI ACTGGG 1 cut(s) 462
BmsI GCATC 2 cut(s) 523, 582
BmuI ACTGGG 1 cut(s) 462
Bpu10I CCTNAGC 1 cut(s) 68
Bpu14I TTCGAA 1 cut(s) 336
BpuMI CCSGG 1 cut(s) 667
BsaHI GRCGYC 1 cut(s) 201
BsaJI CCNNGG 1 cut(s) 513
BsaWI WCCGGW 1 cut(s) 626
Bsc4I CCNNNNNNNGG 1 cut(s) 210
Bse118I RCCGGY 1 cut(s) 626
Bse1I ACTGG 1 cut(s) 468
Bse21I CCTNAGG 1 cut(s) 124
Bse3DI GCAATG 2 cut(s) 22, 498
BseBI CCWGG 3 cut(s) 515, 597, 615
BseDI CCNNGG 1 cut(s) 513
BseGI GGATG 2 cut(s) 597, 662
BseLI CCNNNNNNNGG 1 cut(s) 210
BseMI GCAATG 2 cut(s) 22, 498
BseMII CTCAG 3 cut(s) 82, 138, 416
BseNI ACTGG 1 cut(s) 468
BseRI GAGGAG 3 cut(s) 339, 633, 670
BshNI GGYRCC 1 cut(s) 102
BshTI ACCGGT 1 cut(s) 626
BsiHKAI GWGCWC 2 cut(s) 69, 354
BsiSI CCGG 2 cut(s) 627, 666
BslI CCNNNNNNNGG 1 cut(s) 210
BsmAI GTCTC 1 cut(s) 682
BsmI GAATGC 2 cut(s) 56, 79
Bsp119I TTCGAA 1 cut(s) 336
Bsp1286I GDGCHC 2 cut(s) 69, 354
Bsp143I GATC 3 cut(s) 109, 579, 688
BspACI CCGC 3 cut(s) 287, 489, 563
BspCNI CTCAG 3 cut(s) 81, 137, 415
BspLI GGNNCC 1 cut(s) 104
BspPI GGATC 1 cut(s) 587
BspT104I TTCGAA 1 cut(s) 336
BspT107I GGYRCC 1 cut(s) 102
BsrDI GCAATG 2 cut(s) 22, 498
BsrFI RCCGGY 1 cut(s) 626
BsrI ACTGG 1 cut(s) 468
BssAI RCCGGY 1 cut(s) 626
BssECI CCNNGG 1 cut(s) 513
BssMI GATC 3 cut(s) 109, 579, 688
BssNI GRCGYC 1 cut(s) 201
Bst2UI CCWGG 3 cut(s) 515, 597, 615
Bst4CI ACNGT 2 cut(s) 452, 521
Bst6I CTCTTC 1 cut(s) 595
BstACI GRCGYC 1 cut(s) 201
BstBI TTCGAA 1 cut(s) 336
BstC8I GCNNGC 1 cut(s) 572
BstDEI CTNAG 4 cut(s) 68, 124, 306, 402
BstF5I GGATG 2 cut(s) 597, 662
BstKTI GATC 3 cut(s) 112, 582, 691
BstMAI GTCTC 1 cut(s) 682
BstMBI GATC 3 cut(s) 109, 579, 688
BstNI CCWGG 3 cut(s) 515, 597, 615
BstSCI CCNGG 4 cut(s) 513, 595, 613, 665
Bsu36I CCTNAGG 1 cut(s) 124
BtsCI GGATG 2 cut(s) 597, 662
BtsIMutI CAGTG 1 cut(s) 92
Cac8I GCNNGC 1 cut(s) 572
Cfr10I RCCGGY 1 cut(s) 626
CseI GACGC 1 cut(s) 209
Csp6I GTAC 2 cut(s) 220, 448
CspAI ACCGGT 1 cut(s) 626
CspCI CAANNNNNGTGG 2 cut(s) 123, 158
CviQI GTAC 2 cut(s) 220, 448
DdeI CTNAG 4 cut(s) 68, 124, 306, 402
DpnI GATC 3 cut(s) 111, 581, 690
DpnII GATC 3 cut(s) 109, 579, 688
DraI TTTAAA 1 cut(s) 276
Eam1104I CTCTTC 1 cut(s) 595
EarI CTCTTC 1 cut(s) 595
Ecl136II GAGCTC 2 cut(s) 67, 352
Eco24I GRGCYC 2 cut(s) 69, 354
Eco53kI GAGCTC 2 cut(s) 67, 352
Eco81I CCTNAGG 1 cut(s) 124
EcoICRI GAGCTC 2 cut(s) 67, 352
EcoRII CCWGG 3 cut(s) 513, 595, 613
EcoT38I GRGCYC 2 cut(s) 69, 354
FaiI YATR 7 cut(s) 27, 35, 81, 100, 237, 439, 501
FauI CCCGC 1 cut(s) 556
FbaI TGATCA 1 cut(s) 688
FokI GGATG 2 cut(s) 604, 649
FriOI GRGCYC 2 cut(s) 69, 354
FspBI CTAG 1 cut(s) 708
HapII CCGG 2 cut(s) 627, 666
HgaI GACGC 1 cut(s) 209
Hin1I GRCGYC 1 cut(s) 201
HinfI GANTC 2 cut(s) 59, 610
HpaII CCGG 2 cut(s) 627, 666
HphI GGTGA 2 cut(s) 278, 301
Hpy188I TCNGA 3 cut(s) 58, 64, 199
Hpy188III TCNNGA 4 cut(s) 70, 126, 167, 583
Hpy99I CGWCG 1 cut(s) 203
HpyAV CCTTC 3 cut(s) 40, 580, 630
HpyCH4III ACNGT 2 cut(s) 452, 521
HpyCH4IV ACGT 1 cut(s) 478
HpyCH4V TGCA 2 cut(s) 92, 346
HpyF3I CTNAG 4 cut(s) 68, 124, 306, 402
HpySE526I ACGT 1 cut(s) 478
Hsp92I GRCGYC 1 cut(s) 201
Ksp22I TGATCA 1 cut(s) 688
Kzo9I GATC 3 cut(s) 109, 579, 688
LmnI GCTCC 4 cut(s) 64, 230, 349, 398
LweI GCATC 2 cut(s) 523, 582
MaeI CTAG 1 cut(s) 708
MaeII ACGT 1 cut(s) 478
MaeIII GTNAC 3 cut(s) 470, 622, 658
MalI GATC 3 cut(s) 111, 581, 690
MboI GATC 3 cut(s) 109, 579, 688
MboII GAAGA 7 cut(s) 17, 55, 158, 169, 371, 426, 612
MfeI CAATTG 1 cut(s) 341
MhlI GDGCHC 2 cut(s) 69, 354
MluCI AATT 6 cut(s) 130, 341, 457, 481, 496, 547
MlyI GAGTC 1 cut(s) 53
MmeI TCCRAC 1 cut(s) 222
MroXI GAANNNNTTC 2 cut(s) 366, 609
MseI TTAA 1 cut(s) 275
MslI CAYNNNNRTG 1 cut(s) 97
MspI CCGG 2 cut(s) 627, 666
MspR9I CCNGG 4 cut(s) 515, 597, 615, 667
MunI CAATTG 1 cut(s) 341
Mva1269I GAATGC 2 cut(s) 56, 79
MvaI CCWGG 3 cut(s) 515, 597, 615
NciI CCSGG 1 cut(s) 667
NdeII GATC 3 cut(s) 109, 579, 688
NlaIV GGNNCC 1 cut(s) 104
NspV TTCGAA 1 cut(s) 336
PctI GAATGC 2 cut(s) 56, 79
PdmI GAANNNNTTC 2 cut(s) 366, 609
PfeI GAWTC 1 cut(s) 610
PfoI TCCNGGA 1 cut(s) 613
PinAI ACCGGT 1 cut(s) 626
PleI GAGTC 1 cut(s) 53
PpsI GAGTC 1 cut(s) 53
PsiI TTATAA 1 cut(s) 81
Psp124BI GAGCTC 2 cut(s) 69, 354
Psp6I CCWGG 3 cut(s) 513, 595, 613
PspGI CCWGG 3 cut(s) 513, 595, 613
PspN4I GGNNCC 1 cut(s) 104
RsaI GTAC 2 cut(s) 221, 449
RsaNI GTAC 2 cut(s) 220, 448
RseI CAYNNNNRTG 1 cut(s) 97
SacI GAGCTC 2 cut(s) 69, 354
SaqAI TTAA 1 cut(s) 275
Sau3AI GATC 3 cut(s) 109, 579, 688
SchI GAGTC 1 cut(s) 53
ScrFI CCNGG 4 cut(s) 515, 597, 615, 667
SduI GDGCHC 2 cut(s) 69, 354
SetI ASST 9 cut(s) 69, 215, 235, 335, 354, 395, 481, 660, 709
SfaNI GCATC 2 cut(s) 523, 582
SfuI TTCGAA 1 cut(s) 336
SmiMI CAYNNNNRTG 1 cut(s) 97
Sse9I AATT 6 cut(s) 130, 341, 457, 481, 496, 547
SsiI CCGC 3 cut(s) 287, 489, 563
SspMI CTAG 1 cut(s) 708
SstI GAGCTC 2 cut(s) 69, 354
StyD4I CCNGG 4 cut(s) 513, 595, 613, 665
TaaI ACNGT 2 cut(s) 452, 521
TaiI ACGT 1 cut(s) 481
TaqI TCGA 1 cut(s) 336
TasI AATT 6 cut(s) 130, 341, 457, 481, 496, 547
TatI WGTACW 1 cut(s) 447
TfiI GAWTC 1 cut(s) 610
Tru1I TTAA 1 cut(s) 275
Tru9I TTAA 1 cut(s) 275
TscAI CASTG 1 cut(s) 99
TspDTI ATGAA 4 cut(s) 252, 330, 426, 509
TspRI CASTG 1 cut(s) 99
XapI RAATTY 2 cut(s) 130, 457
XmnI GAANNNNTTC 2 cut(s) 366, 609
XspI CTAG 1 cut(s) 708
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.