MD17G1150300.v1.1

DnaJ homolog subfamily B member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0010346
Physical Location & Seq
Reverse (-)
13829145 .. 13835292
6148 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1150300.v1.1.491

Sequence Viewer

Length: 723 bp
ATGGCTGATGGGGAAGAGAAAGGCAGTGACTTCTATGCAGTTTTAGGGTTGGACAAGGAATGCACAGACTCGGAGCTCAGAAATTCCTATAAGAAACTTGCACTGAGATGGCACCCGGATCGCTGTTCTGCCTCCGGAAATTCTAAGTTCGTGGAAGAAGCCAAGATGAAATTCCAAGACATTCAACAAGCCTATTCTGTTTTATCTGACGCGAATAAGAGGTTTCTGTACGATGTAGGAGCCTATGGCAGTGATGATGACGAAAACAATGGGATGGGTGAGTTTTTGAACGAAATGGCAATGATGATGAGCCAAACAAAGCCTAATGAAAATGGGAAGGAGACGTTTGAAGAATTGCAGGATATTTTTTATGAAATGTTTCAAGGGGATATTGGGAGTTATGGGTCTGCTTCTCGGACCCCTAGTTCCTGTTCTACTTCTTCGTATGCGTCCTATTGCGAAAGCTCAAGTTCCAATAACAAGCGCAATTGCTCTGAAATGAACTATGAGAAGGTGACCTTGGACGATCCTTCTGGCTTCGACACTCATTTTCCAAGTTTTTGTGTAGGGGTGGGAAGCCGTCAAGACAACGGGAAGGCAACGGCAGCAGGAGAAGGGATTCGCGCAAAAACCACCGGTAATCGAAGGCAGGGCAGGAAACAAAAGGTCACATCTGGGCATGATGTTTCTTCAAATGACTACTCTGGTATATCAGCTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0051087
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

241

Amino Acids

26.43

Weight (kDa)

4.89

Isoelectric Point (pI)

49.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 10 - 78 9.5e-20 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 111
AccII CGCG 2 cut(s) 212, 624
AccIII TCCGGA 1 cut(s) 134
AclWI GGATC 2 cut(s) 126, 521
AcsI RAATTY 3 cut(s) 82, 139, 170
AfaI GTAC 1 cut(s) 230
AgeI ACCGGT 1 cut(s) 635
AgsI TTSAA 5 cut(s) 185, 289, 350, 383, 693
AjuI GAANNNNNNNTTGG 4 cut(s) 155, 187, 503, 535
AloI GAACNNNNNNTCC 2 cut(s) 409, 441
AluBI AGCT 3 cut(s) 76, 465, 716
AluI AGCT 3 cut(s) 76, 465, 716
Alw21I GWGCWC 1 cut(s) 78
Alw26I GTCTC 1 cut(s) 335
AlwI GGATC 2 cut(s) 126, 521
Aor13HI TCCGGA 1 cut(s) 134
ApeKI GCWGC 1 cut(s) 605
ApoI RAATTY 3 cut(s) 82, 139, 170
AsiGI ACCGGT 1 cut(s) 635
Asp700I GAANNNNTTC 2 cut(s) 378, 618
AspLEI GCGC 2 cut(s) 486, 626
AspS9I GGNCC 1 cut(s) 417
AsuC2I CCSGG 1 cut(s) 116
AsuHPI GGTGA 2 cut(s) 290, 526
AvaII GGWCC 1 cut(s) 417
BanI GGYRCC 1 cut(s) 111
BanII GRGCYC 1 cut(s) 78
Bbv12I GWGCWC 1 cut(s) 78
BbvI GCAGC 1 cut(s) 617
BccI CCATC 3 cut(s) 2, 102, 268
BceAI ACGGC 2 cut(s) 564, 618
BcgI CGANNNNNNTGC 2 cut(s) 101, 135
BcnI CCSGG 1 cut(s) 116
BcoDI GTCTC 1 cut(s) 335
BfaI CTAG 2 cut(s) 423, 717
BisI GCNGC 1 cut(s) 606
BlsI GCNGC 1 cut(s) 607
Bme1390I CCNGG 1 cut(s) 116
Bme18I GGWCC 1 cut(s) 417
BmgT120I GGNCC 1 cut(s) 417
BmiI GGNNCC 3 cut(s) 113, 241, 419
BmrFI CCNGG 1 cut(s) 116
BpuEI CTTGAG 1 cut(s) 451
BpuMI CCSGG 1 cut(s) 116
BsaJI CCNNGG 1 cut(s) 519
BsaWI WCCGGW 2 cut(s) 134, 635
Bse118I RCCGGY 1 cut(s) 635
Bse3DI GCAATG 1 cut(s) 306
BseAI TCCGGA 1 cut(s) 134
BseDI CCNNGG 1 cut(s) 519
BseGI GGATG 1 cut(s) 279
BseMI GCAATG 1 cut(s) 306
BseMII CTCAG 2 cut(s) 91, 95
BseXI GCAGC 1 cut(s) 617
Bsh1236I CGCG 2 cut(s) 212, 624
BshNI GGYRCC 1 cut(s) 111
BshTI ACCGGT 1 cut(s) 635
BsiHKAI GWGCWC 1 cut(s) 78
BsiSI CCGG 3 cut(s) 116, 135, 636
BsmAI GTCTC 1 cut(s) 335
BsmBI CGTCTC 1 cut(s) 335
BsmI GAATGC 1 cut(s) 65
Bsp1286I GDGCHC 1 cut(s) 78
Bsp13I TCCGGA 1 cut(s) 134
Bsp143I GATC 2 cut(s) 118, 526
BspCNI CTCAG 2 cut(s) 90, 96
BspEI TCCGGA 1 cut(s) 134
BspFNI CGCG 2 cut(s) 212, 624
BspLI GGNNCC 3 cut(s) 113, 241, 419
BspPI GGATC 2 cut(s) 126, 521
BspT107I GGYRCC 1 cut(s) 111
BsrDI GCAATG 1 cut(s) 306
BsrFI RCCGGY 1 cut(s) 635
BssAI RCCGGY 1 cut(s) 635
BssECI CCNNGG 1 cut(s) 519
BssMI GATC 2 cut(s) 118, 526
BssT1I CCWWGG 1 cut(s) 519
Bst6I CTCTTC 1 cut(s) 9
BstDEI CTNAG 3 cut(s) 77, 104, 144
BstEII GGTNACC 1 cut(s) 514
BstF5I GGATG 1 cut(s) 279
BstFNI CGCG 2 cut(s) 212, 624
BstHHI GCGC 2 cut(s) 486, 626
BstKTI GATC 2 cut(s) 121, 529
BstMAI GTCTC 1 cut(s) 335
BstMBI GATC 2 cut(s) 118, 526
BstMWI GCNNNNNNNGC 1 cut(s) 605
BstPI GGTNACC 1 cut(s) 514
BstSCI CCNGG 1 cut(s) 114
BstUI CGCG 2 cut(s) 212, 624
BstV1I GCAGC 1 cut(s) 617
BtsCI GGATG 1 cut(s) 279
BtsI GCAGTG 2 cut(s) 31, 256
BtsIMutI CAGTG 3 cut(s) 31, 101, 256
CfoI GCGC 2 cut(s) 486, 626
Cfr10I RCCGGY 1 cut(s) 635
Cfr13I GGNCC 1 cut(s) 417
CseI GACGC 2 cut(s) 218, 438
Csp6I GTAC 1 cut(s) 229
CspAI ACCGGT 1 cut(s) 635
CviAII CATG 1 cut(s) 680
CviQI GTAC 1 cut(s) 229
DdeI CTNAG 3 cut(s) 77, 104, 144
DpnI GATC 2 cut(s) 120, 528
DpnII GATC 2 cut(s) 118, 526
Eam1104I CTCTTC 1 cut(s) 9
EarI CTCTTC 1 cut(s) 9
Ecl136II GAGCTC 1 cut(s) 76
Eco130I CCWWGG 1 cut(s) 519
Eco24I GRGCYC 1 cut(s) 78
Eco47I GGWCC 1 cut(s) 417
Eco53kI GAGCTC 1 cut(s) 76
Eco91I GGTNACC 1 cut(s) 514
EcoICRI GAGCTC 1 cut(s) 76
EcoO65I GGTNACC 1 cut(s) 514
EcoT14I CCWWGG 1 cut(s) 519
EcoT38I GRGCYC 1 cut(s) 78
ErhI CCWWGG 1 cut(s) 519
Esp3I CGTCTC 1 cut(s) 335
FaeI CATG 1 cut(s) 683
FaiI YATR 9 cut(s) 36, 90, 246, 372, 402, 447, 507, 681, 710
FalI AAGNNNNNCTT 2 cut(s) 503, 535
FatI CATG 1 cut(s) 679
Fnu4HI GCNGC 1 cut(s) 606
FokI GGATG 1 cut(s) 286
FriOI GRGCYC 1 cut(s) 78
Fsp4HI GCNGC 1 cut(s) 606
FspBI CTAG 2 cut(s) 423, 717
GlaI GCGC 2 cut(s) 485, 625
GluI GCNGC 1 cut(s) 606
HapII CCGG 3 cut(s) 116, 135, 636
HgaI GACGC 2 cut(s) 218, 438
HhaI GCGC 2 cut(s) 486, 626
Hin1II CATG 1 cut(s) 683
Hin6I GCGC 2 cut(s) 484, 624
HinP1I GCGC 2 cut(s) 484, 624
HinfI GANTC 2 cut(s) 68, 619
HpaII CCGG 3 cut(s) 116, 135, 636
HphI GGTGA 2 cut(s) 290, 526
Hpy188I TCNGA 5 cut(s) 73, 80, 208, 417, 496
Hpy188III TCNNGA 2 cut(s) 135, 584
HpyAV CCTTC 6 cut(s) 331, 505, 540, 589, 608, 639
HpyCH4IV ACGT 1 cut(s) 344
HpyCH4V TGCA 4 cut(s) 38, 63, 101, 358
HpyF10VI GCNNNNNNNGC 1 cut(s) 605
HpyF3I CTNAG 3 cut(s) 77, 104, 144
HpySE526I ACGT 1 cut(s) 344
Hsp92II CATG 1 cut(s) 683
HspAI GCGC 2 cut(s) 484, 624
Kpn2I TCCGGA 1 cut(s) 134
Kzo9I GATC 2 cut(s) 118, 526
LmnI GCTCC 2 cut(s) 73, 239
Lsp1109I GCAGC 1 cut(s) 617
MaeI CTAG 2 cut(s) 423, 717
MaeII ACGT 1 cut(s) 344
MaeIII GTNAC 3 cut(s) 26, 514, 667
MalI GATC 2 cut(s) 120, 528
MboI GATC 2 cut(s) 118, 526
MboII GAAGA 5 cut(s) 26, 167, 362, 432, 681
MfeI CAATTG 1 cut(s) 487
MhlI GDGCHC 1 cut(s) 78
MluCI AATT 5 cut(s) 82, 139, 170, 353, 487
MlyI GAGTC 1 cut(s) 62
MmeI TCCRAC 1 cut(s) 30
MnlI CCTC 2 cut(s) 142, 213
MroI TCCGGA 1 cut(s) 134
MroXI GAANNNNTTC 2 cut(s) 378, 618
MslI CAYNNNNRTG 1 cut(s) 106
MspI CCGG 3 cut(s) 116, 135, 636
MspR9I CCNGG 1 cut(s) 116
MunI CAATTG 1 cut(s) 487
Mva1269I GAATGC 1 cut(s) 65
MvnI CGCG 2 cut(s) 212, 624
MwoI GCNNNNNNNGC 1 cut(s) 605
NciI CCSGG 1 cut(s) 116
NdeII GATC 2 cut(s) 118, 526
NlaIII CATG 1 cut(s) 683
NlaIV GGNNCC 3 cut(s) 113, 241, 419
NmuCI GTSAC 3 cut(s) 26, 514, 667
PctI GAATGC 1 cut(s) 65
PdmI GAANNNNTTC 2 cut(s) 378, 618
PfeI GAWTC 1 cut(s) 619
PinAI ACCGGT 1 cut(s) 635
PkrI GCNGC 1 cut(s) 607
PleI GAGTC 1 cut(s) 62
PpsI GAGTC 1 cut(s) 62
Psp124BI GAGCTC 1 cut(s) 78
PspEI GGTNACC 1 cut(s) 514
PspN4I GGNNCC 3 cut(s) 113, 241, 419
PspPI GGNCC 1 cut(s) 417
RsaI GTAC 1 cut(s) 230
RsaNI GTAC 1 cut(s) 229
RseI CAYNNNNRTG 1 cut(s) 106
SacI GAGCTC 1 cut(s) 78
SatI GCNGC 1 cut(s) 606
Sau3AI GATC 2 cut(s) 118, 526
Sau96I GGNCC 1 cut(s) 417
SchI GAGTC 1 cut(s) 62
ScrFI CCNGG 1 cut(s) 116
SduI GDGCHC 1 cut(s) 78
SetI ASST 8 cut(s) 78, 224, 347, 467, 516, 521, 669, 718
SinI GGWCC 1 cut(s) 417
SmiMI CAYNNNNRTG 1 cut(s) 106
SmlI CTYRAG 1 cut(s) 466
SmoI CTYRAG 1 cut(s) 466
Sse9I AATT 5 cut(s) 82, 139, 170, 353, 487
SspMI CTAG 2 cut(s) 423, 717
SstI GAGCTC 1 cut(s) 78
StyD4I CCNGG 1 cut(s) 114
StyI CCWWGG 1 cut(s) 519
TaiI ACGT 1 cut(s) 347
TaqI TCGA 2 cut(s) 540, 643
TasI AATT 5 cut(s) 82, 139, 170, 353, 487
TfiI GAWTC 1 cut(s) 619
TscAI CASTG 3 cut(s) 31, 108, 256
TseFI GTSAC 3 cut(s) 26, 514, 667
TseI GCWGC 1 cut(s) 605
Tsp45I GTSAC 3 cut(s) 26, 514, 667
TspDTI ATGAA 4 cut(s) 182, 342, 387, 515
TspRI CASTG 3 cut(s) 31, 108, 256
VpaK11BI GGWCC 1 cut(s) 417
XapI RAATTY 3 cut(s) 82, 139, 170
XmnI GAANNNNTTC 2 cut(s) 378, 618
XspI CTAG 2 cut(s) 423, 717
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.