Rh2AG453300

DnaJ homolog subfamily B member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0010346
Physical Location & Seq
Reverse (-)
66755459 .. 66759635
4177 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG453300.1

Sequence Viewer

Length: 633 bp
ATGGCTAATAGGGAAGAGAAAGGCAATGACTTTTATGCTGTTTTGGGGTTGAAGAAGGAATGCTCGGATTCGGAGCTCAGGAATGCCTATAAGAAACTTGCACTGAGATGGCACCCAGATCGTTGCTCGGCCTCAGGAAATTCTAAGTTCGTGGAAGAAGCCAAGAAGAAGTTTCAGGACATTCAACAAGCCTATTCTGTTCTGTCTGACGCGAACAAGAGATTTATGTACGATGTAGGAGCTTATGAAGGTGATGATGACGAAAATGGAATGGGTGATTTTTTGAACGAGATGGCGGTGATGATGAGCCAGACTAAGCCAAATGAAAATGGAGGGGAGAGCTTTGAACAATTGCAGGAACTCTTTGAAGAAATGTTTCAGGGGGATATTGAGAGTTTCAGCTCCTGCTCTCAGCCTCCTGCTTCCTGTTCTACTTCTTCATCTTCATACGCATCCTACTGTGAAAGTTCTACACCCAATAACAAACGTAATTCCTCTGAAATGAATTATGGCAACGCAACCTTGGACAGTTCTGGTTTTGACACTCATTTTCACAGTTTTTGTGTAGGGGTTAGTGCTCTTGCCTTTATGCTTTTAAATCTAATTTTTGAGCATTTTATGTTTTTTATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0051087
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

210

Amino Acids

23.62

Weight (kDa)

4.64

Isoelectric Point (pI)

56.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 10 - 78 2.6e-21 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 111
AccII CGCG 1 cut(s) 212
AciI CCGC 1 cut(s) 296
AcsI RAATTY 1 cut(s) 139
AfaI GTAC 1 cut(s) 230
AgsI TTSAA 5 cut(s) 52, 185, 286, 347, 368
AluBI AGCT 4 cut(s) 76, 242, 342, 402
AluI AGCT 4 cut(s) 76, 242, 342, 402
Alw21I GWGCWC 2 cut(s) 78, 580
AlwNI CAGNNNCTG 1 cut(s) 405
AoxI GGCC 1 cut(s) 129
ApoI RAATTY 1 cut(s) 139
Asp700I GAANNNNTTC 1 cut(s) 375
AsuHPI GGTGA 3 cut(s) 263, 287, 310
AxyI CCTNAGG 1 cut(s) 133
BanI GGYRCC 1 cut(s) 111
BanII GRGCYC 1 cut(s) 78
Bbv12I GWGCWC 2 cut(s) 78, 580
BccI CCATC 2 cut(s) 102, 286
BcgI CGANNNNNNTGC 2 cut(s) 101, 135
BmiI GGNNCC 1 cut(s) 113
BmsI GCATC 1 cut(s) 461
Bpu10I CCTNAGC 1 cut(s) 77
BsaJI CCNNGG 1 cut(s) 522
Bse21I CCTNAGG 1 cut(s) 133
Bse3DI GCAATG 1 cut(s) 31
BseDI CCNNGG 1 cut(s) 522
BseGI GGATG 1 cut(s) 452
BseMI GCAATG 1 cut(s) 31
BseMII CTCAG 4 cut(s) 91, 95, 147, 425
Bsh1236I CGCG 1 cut(s) 212
BshFI GGCC 1 cut(s) 131
BshNI GGYRCC 1 cut(s) 111
BsiHKAI GWGCWC 2 cut(s) 78, 580
BsmI GAATGC 2 cut(s) 65, 88
BsnI GGCC 1 cut(s) 131
Bsp1286I GDGCHC 2 cut(s) 78, 580
Bsp143I GATC 1 cut(s) 118
BspACI CCGC 1 cut(s) 296
BspANI GGCC 1 cut(s) 131
BspCNI CTCAG 4 cut(s) 90, 96, 146, 424
BspFNI CGCG 1 cut(s) 212
BspLI GGNNCC 1 cut(s) 113
BspT107I GGYRCC 1 cut(s) 111
BsrDI GCAATG 1 cut(s) 31
BssECI CCNNGG 1 cut(s) 522
BssMI GATC 1 cut(s) 118
BssT1I CCWWGG 1 cut(s) 522
Bst4CI ACNGT 3 cut(s) 461, 530, 557
Bst6I CTCTTC 1 cut(s) 9
BstDEI CTNAG 6 cut(s) 77, 104, 133, 144, 315, 411
BstF5I GGATG 1 cut(s) 452
BstFNI CGCG 1 cut(s) 212
BstKTI GATC 1 cut(s) 121
BstMBI GATC 1 cut(s) 118
BstUI CGCG 1 cut(s) 212
Bsu36I CCTNAGG 1 cut(s) 133
BsuRI GGCC 1 cut(s) 131
BtsCI GGATG 1 cut(s) 452
BtsIMutI CAGTG 1 cut(s) 101
CaiI CAGNNNCTG 1 cut(s) 405
CseI GACGC 1 cut(s) 218
Csp6I GTAC 1 cut(s) 229
CviQI GTAC 1 cut(s) 229
DdeI CTNAG 6 cut(s) 77, 104, 133, 144, 315, 411
DpnI GATC 1 cut(s) 120
DpnII GATC 1 cut(s) 118
DraI TTTAAA 1 cut(s) 597
Eam1104I CTCTTC 1 cut(s) 9
EarI CTCTTC 1 cut(s) 9
Ecl136II GAGCTC 1 cut(s) 76
Eco130I CCWWGG 1 cut(s) 522
Eco24I GRGCYC 1 cut(s) 78
Eco53kI GAGCTC 1 cut(s) 76
Eco81I CCTNAGG 1 cut(s) 133
EcoICRI GAGCTC 1 cut(s) 76
EcoT14I CCWWGG 1 cut(s) 522
EcoT38I GRGCYC 1 cut(s) 78
ErhI CCWWGG 1 cut(s) 522
FokI GGATG 1 cut(s) 439
FriOI GRGCYC 1 cut(s) 78
HaeIII GGCC 1 cut(s) 131
HgaI GACGC 1 cut(s) 218
HinfI GANTC 1 cut(s) 68
HphI GGTGA 3 cut(s) 263, 287, 310
Hpy188I TCNGA 4 cut(s) 67, 73, 208, 499
Hpy188III TCNNGA 3 cut(s) 79, 135, 176
HpyAV CCTTC 2 cut(s) 49, 242
HpyCH4III ACNGT 3 cut(s) 461, 530, 557
HpyCH4IV ACGT 1 cut(s) 487
HpyCH4V TGCA 2 cut(s) 101, 355
HpyF3I CTNAG 6 cut(s) 77, 104, 133, 144, 315, 411
HpySE526I ACGT 1 cut(s) 487
Kzo9I GATC 1 cut(s) 118
LmnI GCTCC 3 cut(s) 73, 239, 407
LweI GCATC 1 cut(s) 461
MaeII ACGT 1 cut(s) 487
MalI GATC 1 cut(s) 120
MboI GATC 1 cut(s) 118
MboII GAAGA 7 cut(s) 26, 64, 167, 178, 380, 429, 435
MfeI CAATTG 1 cut(s) 350
MhlI GDGCHC 2 cut(s) 78, 580
MluCI AATT 5 cut(s) 139, 350, 490, 505, 603
MnlI CCTC 4 cut(s) 142, 326, 426, 505
MroXI GAANNNNTTC 1 cut(s) 375
MseI TTAA 1 cut(s) 596
MslI CAYNNNNRTG 1 cut(s) 106
MunI CAATTG 1 cut(s) 350
Mva1269I GAATGC 2 cut(s) 65, 88
MvnI CGCG 1 cut(s) 212
NdeII GATC 1 cut(s) 118
NlaIV GGNNCC 1 cut(s) 113
NmeAIII GCCGAG 1 cut(s) 107
PctI GAATGC 2 cut(s) 65, 88
PdmI GAANNNNTTC 1 cut(s) 375
PfeI GAWTC 1 cut(s) 68
Psp124BI GAGCTC 1 cut(s) 78
PspN4I GGNNCC 1 cut(s) 113
PstNI CAGNNNCTG 1 cut(s) 405
RsaI GTAC 1 cut(s) 230
RsaNI GTAC 1 cut(s) 229
RseI CAYNNNNRTG 1 cut(s) 106
SacI GAGCTC 1 cut(s) 78
SaqAI TTAA 1 cut(s) 596
Sau3AI GATC 1 cut(s) 118
SduI GDGCHC 2 cut(s) 78, 580
SetI ASST 7 cut(s) 78, 244, 253, 344, 404, 490, 524
SfaNI GCATC 1 cut(s) 461
SmiMI CAYNNNNRTG 1 cut(s) 106
Sse9I AATT 5 cut(s) 139, 350, 490, 505, 603
SsiI CCGC 1 cut(s) 296
SstI GAGCTC 1 cut(s) 78
StyI CCWWGG 1 cut(s) 522
TaaI ACNGT 3 cut(s) 461, 530, 557
TaiI ACGT 1 cut(s) 490
TasI AATT 5 cut(s) 139, 350, 490, 505, 603
TfiI GAWTC 1 cut(s) 68
Tru1I TTAA 1 cut(s) 596
Tru9I TTAA 1 cut(s) 596
TscAI CASTG 1 cut(s) 108
TspDTI ATGAA 5 cut(s) 261, 339, 429, 435, 518
TspRI CASTG 1 cut(s) 108
XapI RAATTY 1 cut(s) 139
XmnI GAANNNNTTC 1 cut(s) 375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.