RLG00000020222

DnaJ homolog subfamily B member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0010346
Physical Location & Seq
Reverse (-)
62619334 .. 62622310
2977 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020222

Sequence Viewer

Length: 645 bp
ATGGCTAATAGGGAAGAGAAAGGCAATGACTTTTATGCTGTTTTGGGGTTGAAGAAGGAATGCTCGGACTCGGAGCTCAGGAATGCCTATAAGAAACTTGCACTGAGATGGCACCCAGATCGTTGCTCGGCCTCAGGAAATTCAAAGTTCGTGGAAGAAGCCAAGAAGAAGTTTCAGGACATTCAACAAGCCTATTCTGTTTTGTCTGACGCGAACAAGAGATTTATGTACGATGTAGGAGCTTATGAAAGTGATGATGACGAAAATGGAATGGGTGATTTTTTGAACGAGATGGCGGTGATGATGAGCCAGACCAAGCCGAATGAAAATGGAGGGGAGAGCTTTGAACAATTGCAGGAACTCTTTGAAGAAATGTTTCAGGGGGATATTGAGAGTTTTAGCTCCTGCTCCCAGCCTCCTGCTTCCTGTTCTACTTCTTCATCTTCATACGCATCCTACTGTGAAAGTTCTACACCCAATAACAAACGTAATTCCTCTGAAATGAATTATGGCAACGCAACCTTGGACAGTTCTGGTTTTGACACTCATTTTCACAGTTTTTGTGTAGGGACAGGCGGAAAGCCAGCAAGGAATCAGGAAAGGGATGCCGGGAAGAGAAGGGATTCCAGGAGGAGTAACCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0051087
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

215

Amino Acids

24.01

Weight (kDa)

5.14

Isoelectric Point (pI)

68.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 10 - 78 2.7e-21 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 111
AccII CGCG 1 cut(s) 212
AciI CCGC 2 cut(s) 296, 576
AcsI RAATTY 1 cut(s) 139
AfaI GTAC 1 cut(s) 230
AgeI ACCGGT 1 cut(s) 639
AgsI TTSAA 6 cut(s) 52, 144, 185, 286, 347, 368
AjnI CCWGG 1 cut(s) 626
AluBI AGCT 4 cut(s) 76, 242, 342, 402
AluI AGCT 4 cut(s) 76, 242, 342, 402
Alw21I GWGCWC 1 cut(s) 78
AoxI GGCC 1 cut(s) 129
ApoI RAATTY 1 cut(s) 139
AsiGI ACCGGT 1 cut(s) 639
Asp700I GAANNNNTTC 2 cut(s) 375, 622
AsuC2I CCSGG 1 cut(s) 610
AsuHPI GGTGA 2 cut(s) 287, 310
AxyI CCTNAGG 1 cut(s) 133
BanI GGYRCC 1 cut(s) 111
BanII GRGCYC 1 cut(s) 78
Bbv12I GWGCWC 1 cut(s) 78
BccI CCATC 2 cut(s) 102, 286
BcgI CGANNNNNNTGC 2 cut(s) 101, 135
BciT130I CCWGG 1 cut(s) 628
BcnI CCSGG 1 cut(s) 610
Bme1390I CCNGG 2 cut(s) 610, 628
BmiI GGNNCC 1 cut(s) 113
BmrFI CCNGG 2 cut(s) 610, 628
BmsI GCATC 2 cut(s) 461, 595
Bpu10I CCTNAGC 1 cut(s) 77
BpuMI CCSGG 1 cut(s) 610
BsaJI CCNNGG 1 cut(s) 522
BsaWI WCCGGW 1 cut(s) 639
Bse118I RCCGGY 1 cut(s) 639
Bse21I CCTNAGG 1 cut(s) 133
Bse3DI GCAATG 1 cut(s) 31
BseBI CCWGG 1 cut(s) 628
BseDI CCNNGG 1 cut(s) 522
BseGI GGATG 2 cut(s) 452, 610
BseMI GCAATG 1 cut(s) 31
BseMII CTCAG 3 cut(s) 91, 95, 147
BseYI CCCAGC 1 cut(s) 411
Bsh1236I CGCG 1 cut(s) 212
BshFI GGCC 1 cut(s) 131
BshNI GGYRCC 1 cut(s) 111
BshTI ACCGGT 1 cut(s) 639
BsiHKAI GWGCWC 1 cut(s) 78
BsiSI CCGG 2 cut(s) 609, 640
BslFI GGGAC 1 cut(s) 583
BsmFI GGGAC 1 cut(s) 583
BsmI GAATGC 2 cut(s) 65, 88
BsnI GGCC 1 cut(s) 131
Bsp1286I GDGCHC 1 cut(s) 78
Bsp143I GATC 1 cut(s) 118
BspACI CCGC 2 cut(s) 296, 576
BspANI GGCC 1 cut(s) 131
BspCNI CTCAG 3 cut(s) 90, 96, 146
BspFNI CGCG 1 cut(s) 212
BspLI GGNNCC 1 cut(s) 113
BspT107I GGYRCC 1 cut(s) 111
BsrDI GCAATG 1 cut(s) 31
BsrFI RCCGGY 1 cut(s) 639
BssAI RCCGGY 1 cut(s) 639
BssECI CCNNGG 1 cut(s) 522
BssMI GATC 1 cut(s) 118
BssT1I CCWWGG 1 cut(s) 522
Bst2UI CCWGG 1 cut(s) 628
Bst4CI ACNGT 3 cut(s) 461, 530, 557
Bst6I CTCTTC 2 cut(s) 9, 608
BstC8I GCNNGC 1 cut(s) 585
BstDEI CTNAG 3 cut(s) 77, 104, 133
BstF5I GGATG 2 cut(s) 452, 610
BstFNI CGCG 1 cut(s) 212
BstKTI GATC 1 cut(s) 121
BstMBI GATC 1 cut(s) 118
BstNI CCWGG 1 cut(s) 628
BstSCI CCNGG 2 cut(s) 608, 626
BstUI CGCG 1 cut(s) 212
Bsu36I CCTNAGG 1 cut(s) 133
BsuRI GGCC 1 cut(s) 131
BtsCI GGATG 2 cut(s) 452, 610
BtsIMutI CAGTG 1 cut(s) 101
Cac8I GCNNGC 1 cut(s) 585
Cfr10I RCCGGY 1 cut(s) 639
CseI GACGC 1 cut(s) 218
Csp6I GTAC 1 cut(s) 229
CspAI ACCGGT 1 cut(s) 639
CspCI CAANNNNNGTGG 2 cut(s) 132, 167
CviQI GTAC 1 cut(s) 229
DdeI CTNAG 3 cut(s) 77, 104, 133
DpnI GATC 1 cut(s) 120
DpnII GATC 1 cut(s) 118
Eam1104I CTCTTC 2 cut(s) 9, 608
EarI CTCTTC 2 cut(s) 9, 608
EciI GGCGGA 1 cut(s) 591
Ecl136II GAGCTC 1 cut(s) 76
Eco130I CCWWGG 1 cut(s) 522
Eco24I GRGCYC 1 cut(s) 78
Eco53kI GAGCTC 1 cut(s) 76
Eco81I CCTNAGG 1 cut(s) 133
EcoICRI GAGCTC 1 cut(s) 76
EcoRII CCWGG 1 cut(s) 626
EcoT14I CCWWGG 1 cut(s) 522
EcoT38I GRGCYC 1 cut(s) 78
ErhI CCWWGG 1 cut(s) 522
FaiI YATR 6 cut(s) 36, 90, 227, 246, 448, 510
FaqI GGGAC 1 cut(s) 583
FokI GGATG 2 cut(s) 439, 617
FriOI GRGCYC 1 cut(s) 78
GsaI CCCAGC 1 cut(s) 415
HaeIII GGCC 1 cut(s) 131
HapII CCGG 2 cut(s) 609, 640
HgaI GACGC 1 cut(s) 218
HinfI GANTC 3 cut(s) 68, 592, 623
HpaII CCGG 2 cut(s) 609, 640
HphI GGTGA 2 cut(s) 287, 310
Hpy188I TCNGA 4 cut(s) 67, 73, 208, 499
Hpy188III TCNNGA 4 cut(s) 79, 135, 176, 596
HpyAV CCTTC 2 cut(s) 49, 612
HpyCH4III ACNGT 3 cut(s) 461, 530, 557
HpyCH4IV ACGT 1 cut(s) 487
HpyCH4V TGCA 2 cut(s) 101, 355
HpyF3I CTNAG 3 cut(s) 77, 104, 133
HpySE526I ACGT 1 cut(s) 487
Kzo9I GATC 1 cut(s) 118
LmnI GCTCC 4 cut(s) 73, 239, 407, 413
LweI GCATC 2 cut(s) 461, 595
MaeII ACGT 1 cut(s) 487
MaeIII GTNAC 1 cut(s) 635
MalI GATC 1 cut(s) 120
MboI GATC 1 cut(s) 118
MboII GAAGA 8 cut(s) 26, 64, 167, 178, 380, 429, 435, 625
MfeI CAATTG 1 cut(s) 350
MhlI GDGCHC 1 cut(s) 78
MluCI AATT 4 cut(s) 139, 350, 490, 505
MlyI GAGTC 1 cut(s) 62
MnlI CCTC 5 cut(s) 142, 326, 426, 505, 624
MroXI GAANNNNTTC 2 cut(s) 375, 622
MslI CAYNNNNRTG 1 cut(s) 106
MspI CCGG 2 cut(s) 609, 640
MspR9I CCNGG 2 cut(s) 610, 628
MunI CAATTG 1 cut(s) 350
Mva1269I GAATGC 2 cut(s) 65, 88
MvaI CCWGG 1 cut(s) 628
MvnI CGCG 1 cut(s) 212
NciI CCSGG 1 cut(s) 610
NdeII GATC 1 cut(s) 118
NlaIV GGNNCC 1 cut(s) 113
NmeAIII GCCGAG 1 cut(s) 107
PctI GAATGC 2 cut(s) 65, 88
PdmI GAANNNNTTC 2 cut(s) 375, 622
PfeI GAWTC 2 cut(s) 592, 623
PfoI TCCNGGA 1 cut(s) 626
PinAI ACCGGT 1 cut(s) 639
PleI GAGTC 1 cut(s) 62
PpsI GAGTC 1 cut(s) 62
Psp124BI GAGCTC 1 cut(s) 78
Psp6I CCWGG 1 cut(s) 626
PspFI CCCAGC 1 cut(s) 411
PspGI CCWGG 1 cut(s) 626
PspN4I GGNNCC 1 cut(s) 113
RsaI GTAC 1 cut(s) 230
RsaNI GTAC 1 cut(s) 229
RseI CAYNNNNRTG 1 cut(s) 106
SacI GAGCTC 1 cut(s) 78
Sau3AI GATC 1 cut(s) 118
SchI GAGTC 1 cut(s) 62
ScrFI CCNGG 2 cut(s) 610, 628
SduI GDGCHC 1 cut(s) 78
SetI ASST 6 cut(s) 78, 244, 344, 404, 490, 524
SfaNI GCATC 2 cut(s) 461, 595
SmiMI CAYNNNNRTG 1 cut(s) 106
Sse9I AATT 4 cut(s) 139, 350, 490, 505
SsiI CCGC 2 cut(s) 296, 576
SstI GAGCTC 1 cut(s) 78
StyD4I CCNGG 2 cut(s) 608, 626
StyI CCWWGG 1 cut(s) 522
TaaI ACNGT 3 cut(s) 461, 530, 557
TaiI ACGT 1 cut(s) 490
TasI AATT 4 cut(s) 139, 350, 490, 505
TfiI GAWTC 2 cut(s) 592, 623
TscAI CASTG 1 cut(s) 108
TspDTI ATGAA 5 cut(s) 261, 339, 429, 435, 518
TspRI CASTG 1 cut(s) 108
XapI RAATTY 1 cut(s) 139
XmnI GAANNNNTTC 2 cut(s) 375, 622
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.