MD09G1176500.v1.1

DnaJ homolog subfamily B member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0010346
Physical Location & Seq
Forward (+)
15094858 .. 15099409
4552 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1176500.v1.1.491

Sequence Viewer

Length: 723 bp
ATGGCGGATGGGGAAGAGAAAGGCAGTGACTTCTATGCAGTTTTGGGGTTGGAAAAGGAATGCACAGACTCGGAGCTCAGAAATGCCTATAAGAAACTTGCACTGAGATGGCACCCGGATCGGTGTTCGGCCTCCGGAAATTCTAAGTTCGTGGAGGAAGCCAAGAAGAAATTCCAGGACATCCAAGAAGCCTATTCTGTTTTATCTGACGCGAATAAGAGGTTTCTGTACGATGTTGGAGCCTACGGCAGTGATGATGACGAAAACAATGGGATGGGTGAGTTTTTGGACGAGATGGCAATGATGATGCGCCAAACGAAGCCTAATGAAAATGGGCAGGAGACCTTTGAAGAATTGCAAAATCTCTTTGATGAAATGTTTGAAGGGGATACTGGGAGTTATGGGCCAGTCTCTCACCCTCCTAGTTCCTGTTCTACGTCTTCATATGTGTCCTACTGTGAAAGTTCAAGTTCCAGTACTAAGCGGAATTGCTCTGAAATGAACTATGAGAAGGCGACCTTGGACGATCCTTCTGGCTTCGACACTCAATTTCAGAGCTTTTGTTTTGGGGTGGAAAGCCTTCAAGACATCGGGGAGGGGACGGCAGCAAGAGAAGTGACTCGCGCAGAAGCCACCGGTAATCGAAGGCATGGCAGGAAACAAAAGGTTACATCTGGTAATGATGTTTCTTCAAATGACTACTCTGGTATATCAGCTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0051087
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

241

Amino Acids

26.65

Weight (kDa)

4.6

Isoelectric Point (pI)

46.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 10 - 78 4e-21 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 111
AccII CGCG 2 cut(s) 212, 624
AccIII TCCGGA 1 cut(s) 134
AciI CCGC 2 cut(s) 5, 484
AclWI GGATC 2 cut(s) 126, 521
AcsI RAATTY 2 cut(s) 139, 170
AfaI GTAC 2 cut(s) 230, 478
AgeI ACCGGT 1 cut(s) 635
AgsI TTSAA 5 cut(s) 350, 383, 468, 584, 693
AjnI CCWGG 1 cut(s) 174
AjuI GAANNNNNNNTTGG 4 cut(s) 155, 187, 503, 535
AluBI AGCT 3 cut(s) 76, 558, 716
AluI AGCT 3 cut(s) 76, 558, 716
Alw21I GWGCWC 1 cut(s) 78
Alw26I GTCTC 2 cut(s) 335, 415
AlwI GGATC 2 cut(s) 126, 521
Aor13HI TCCGGA 1 cut(s) 134
AoxI GGCC 2 cut(s) 129, 404
ApeKI GCWGC 1 cut(s) 605
ApoI RAATTY 2 cut(s) 139, 170
AsiGI ACCGGT 1 cut(s) 635
Asp700I GAANNNNTTC 2 cut(s) 170, 579
AspLEI GCGC 2 cut(s) 312, 626
AspS9I GGNCC 1 cut(s) 404
AsuC2I CCSGG 1 cut(s) 116
AsuHPI GGTGA 2 cut(s) 290, 407
BanI GGYRCC 1 cut(s) 111
BanII GRGCYC 1 cut(s) 78
BbsI GAAGAC 1 cut(s) 432
Bbv12I GWGCWC 1 cut(s) 78
BbvI GCAGC 1 cut(s) 617
BccI CCATC 4 cut(s) 2, 102, 268, 289
BceAI ACGGC 2 cut(s) 262, 618
BcgI CGANNNNNNTGC 2 cut(s) 101, 135
BciT130I CCWGG 1 cut(s) 176
BciVI GTATCC 1 cut(s) 382
BcnI CCSGG 1 cut(s) 116
BcoDI GTCTC 2 cut(s) 335, 415
BfaI CTAG 2 cut(s) 423, 717
BfuI GTATCC 1 cut(s) 382
BisI GCNGC 1 cut(s) 606
BlsI GCNGC 1 cut(s) 607
BmcAI AGTACT 1 cut(s) 478
Bme1390I CCNGG 2 cut(s) 116, 176
BmgT120I GGNCC 1 cut(s) 404
BmiI GGNNCC 2 cut(s) 113, 241
BmrFI CCNGG 2 cut(s) 116, 176
BmrI ACTGGG 1 cut(s) 402
BmsI GCATC 1 cut(s) 297
BmuI ACTGGG 1 cut(s) 402
BpiI GAAGAC 1 cut(s) 432
BpuMI CCSGG 1 cut(s) 116
BsaI GGTCTC 1 cut(s) 335
BsaJI CCNNGG 1 cut(s) 519
BsaWI WCCGGW 2 cut(s) 134, 635
Bse118I RCCGGY 1 cut(s) 635
Bse1I ACTGG 3 cut(s) 397, 407, 474
Bse3DI GCAATG 1 cut(s) 306
BseAI TCCGGA 1 cut(s) 134
BseBI CCWGG 1 cut(s) 176
BseDI CCNNGG 1 cut(s) 519
BseGI GGATG 3 cut(s) 13, 180, 279
BseMI GCAATG 1 cut(s) 306
BseMII CTCAG 2 cut(s) 91, 95
BseNI ACTGG 3 cut(s) 397, 407, 474
BseXI GCAGC 1 cut(s) 617
Bsh1236I CGCG 2 cut(s) 212, 624
BshFI GGCC 2 cut(s) 131, 406
BshNI GGYRCC 1 cut(s) 111
BshTI ACCGGT 1 cut(s) 635
BsiHKAI GWGCWC 1 cut(s) 78
BsiSI CCGG 3 cut(s) 116, 135, 636
BslFI GGGAC 1 cut(s) 613
BsmAI GTCTC 2 cut(s) 335, 415
BsmFI GGGAC 1 cut(s) 613
BsmI GAATGC 1 cut(s) 65
BsnI GGCC 2 cut(s) 131, 406
Bso31I GGTCTC 1 cut(s) 335
Bsp1286I GDGCHC 1 cut(s) 78
Bsp13I TCCGGA 1 cut(s) 134
Bsp143I GATC 2 cut(s) 118, 526
BspACI CCGC 2 cut(s) 5, 484
BspANI GGCC 2 cut(s) 131, 406
BspCNI CTCAG 2 cut(s) 90, 96
BspEI TCCGGA 1 cut(s) 134
BspFNI CGCG 2 cut(s) 212, 624
BspLI GGNNCC 2 cut(s) 113, 241
BspPI GGATC 2 cut(s) 126, 521
BspT107I GGYRCC 1 cut(s) 111
BspTNI GGTCTC 1 cut(s) 335
BsrDI GCAATG 1 cut(s) 306
BsrFI RCCGGY 1 cut(s) 635
BsrI ACTGG 3 cut(s) 397, 407, 474
BssAI RCCGGY 1 cut(s) 635
BssECI CCNNGG 1 cut(s) 519
BssMI GATC 2 cut(s) 118, 526
BssT1I CCWWGG 1 cut(s) 519
Bst2UI CCWGG 1 cut(s) 176
Bst4CI ACNGT 1 cut(s) 458
Bst6I CTCTTC 1 cut(s) 9
BstDEI CTNAG 4 cut(s) 77, 104, 144, 480
BstF5I GGATG 3 cut(s) 13, 180, 279
BstFNI CGCG 2 cut(s) 212, 624
BstHHI GCGC 2 cut(s) 312, 626
BstKTI GATC 2 cut(s) 121, 529
BstMAI GTCTC 2 cut(s) 335, 415
BstMBI GATC 2 cut(s) 118, 526
BstNI CCWGG 1 cut(s) 176
BstSCI CCNGG 2 cut(s) 114, 174
BstUI CGCG 2 cut(s) 212, 624
BstV1I GCAGC 1 cut(s) 617
BstV2I GAAGAC 1 cut(s) 432
BsuI GTATCC 1 cut(s) 382
BsuRI GGCC 2 cut(s) 131, 406
BtsCI GGATG 3 cut(s) 13, 180, 279
BtsI GCAGTG 2 cut(s) 31, 256
BtsIMutI CAGTG 3 cut(s) 31, 101, 256
CfoI GCGC 2 cut(s) 312, 626
Cfr10I RCCGGY 1 cut(s) 635
Cfr13I GGNCC 1 cut(s) 404
CseI GACGC 1 cut(s) 218
Csp6I GTAC 2 cut(s) 229, 477
CspAI ACCGGT 1 cut(s) 635
CviAII CATG 1 cut(s) 650
CviQI GTAC 2 cut(s) 229, 477
DdeI CTNAG 4 cut(s) 77, 104, 144, 480
DpnI GATC 2 cut(s) 120, 528
DpnII GATC 2 cut(s) 118, 526
Eam1104I CTCTTC 1 cut(s) 9
EarI CTCTTC 1 cut(s) 9
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 76
Eco130I CCWWGG 1 cut(s) 519
Eco24I GRGCYC 1 cut(s) 78
Eco31I GGTCTC 1 cut(s) 335
Eco53kI GAGCTC 1 cut(s) 76
EcoICRI GAGCTC 1 cut(s) 76
EcoRII CCWGG 1 cut(s) 174
EcoT14I CCWWGG 1 cut(s) 519
EcoT38I GRGCYC 1 cut(s) 78
ErhI CCWWGG 1 cut(s) 519
FaeI CATG 1 cut(s) 653
FaiI YATR 8 cut(s) 36, 90, 402, 445, 447, 507, 651, 710
FalI AAGNNNNNCTT 2 cut(s) 503, 535
FaqI GGGAC 1 cut(s) 613
FatI CATG 1 cut(s) 649
FauNDI CATATG 1 cut(s) 445
Fnu4HI GCNGC 1 cut(s) 606
FokI GGATG 3 cut(s) 20, 167, 286
FriOI GRGCYC 1 cut(s) 78
Fsp4HI GCNGC 1 cut(s) 606
FspBI CTAG 2 cut(s) 423, 717
GlaI GCGC 2 cut(s) 311, 625
GluI GCNGC 1 cut(s) 606
HaeIII GGCC 2 cut(s) 131, 406
HapII CCGG 3 cut(s) 116, 135, 636
HgaI GACGC 1 cut(s) 218
HhaI GCGC 2 cut(s) 312, 626
Hin1II CATG 1 cut(s) 653
Hin6I GCGC 2 cut(s) 310, 624
HinP1I GCGC 2 cut(s) 310, 624
HinfI GANTC 2 cut(s) 68, 619
HpaII CCGG 3 cut(s) 116, 135, 636
HphI GGTGA 2 cut(s) 290, 407
Hpy188I TCNGA 5 cut(s) 73, 80, 208, 496, 555
Hpy188III TCNNGA 2 cut(s) 135, 584
HpyAV CCTTC 5 cut(s) 377, 505, 540, 590, 639
HpyCH4III ACNGT 1 cut(s) 458
HpyCH4IV ACGT 1 cut(s) 437
HpyCH4V TGCA 4 cut(s) 38, 63, 101, 358
HpyF3I CTNAG 4 cut(s) 77, 104, 144, 480
HpySE526I ACGT 1 cut(s) 437
Hsp92II CATG 1 cut(s) 653
HspAI GCGC 2 cut(s) 310, 624
Kpn2I TCCGGA 1 cut(s) 134
Kzo9I GATC 2 cut(s) 118, 526
LmnI GCTCC 2 cut(s) 73, 239
Lsp1109I GCAGC 1 cut(s) 617
LweI GCATC 1 cut(s) 297
MaeI CTAG 2 cut(s) 423, 717
MaeII ACGT 1 cut(s) 437
MaeIII GTNAC 3 cut(s) 26, 616, 667
MalI GATC 2 cut(s) 120, 528
MboI GATC 2 cut(s) 118, 526
MboII GAAGA 5 cut(s) 26, 178, 362, 432, 681
MhlI GDGCHC 1 cut(s) 78
MluCI AATT 5 cut(s) 139, 170, 353, 487, 548
MlyI GAGTC 2 cut(s) 62, 613
MmeI TCCRAC 2 cut(s) 30, 217
MnlI CCTC 5 cut(s) 142, 148, 213, 429, 589
MroI TCCGGA 1 cut(s) 134
MroXI GAANNNNTTC 2 cut(s) 170, 579
MslI CAYNNNNRTG 1 cut(s) 106
MspI CCGG 3 cut(s) 116, 135, 636
MspR9I CCNGG 2 cut(s) 116, 176
Mva1269I GAATGC 1 cut(s) 65
MvaI CCWGG 1 cut(s) 176
MvnI CGCG 2 cut(s) 212, 624
NciI CCSGG 1 cut(s) 116
NdeI CATATG 1 cut(s) 445
NdeII GATC 2 cut(s) 118, 526
NlaIII CATG 1 cut(s) 653
NlaIV GGNNCC 2 cut(s) 113, 241
NmuCI GTSAC 2 cut(s) 26, 616
PctI GAATGC 1 cut(s) 65
PdmI GAANNNNTTC 2 cut(s) 170, 579
PfoI TCCNGGA 1 cut(s) 174
PinAI ACCGGT 1 cut(s) 635
PkrI GCNGC 1 cut(s) 607
PleI GAGTC 2 cut(s) 62, 613
PpsI GAGTC 2 cut(s) 62, 613
Psp124BI GAGCTC 1 cut(s) 78
Psp6I CCWGG 1 cut(s) 174
PspGI CCWGG 1 cut(s) 174
PspN4I GGNNCC 2 cut(s) 113, 241
PspPI GGNCC 1 cut(s) 404
RsaI GTAC 2 cut(s) 230, 478
RsaNI GTAC 2 cut(s) 229, 477
RseI CAYNNNNRTG 1 cut(s) 106
SacI GAGCTC 1 cut(s) 78
SatI GCNGC 1 cut(s) 606
Sau3AI GATC 2 cut(s) 118, 526
Sau96I GGNCC 1 cut(s) 404
ScaI AGTACT 1 cut(s) 478
SchI GAGTC 2 cut(s) 62, 613
ScrFI CCNGG 2 cut(s) 116, 176
SduI GDGCHC 1 cut(s) 78
SetI ASST 8 cut(s) 78, 224, 347, 440, 521, 560, 669, 718
SfaNI GCATC 1 cut(s) 297
SmiMI CAYNNNNRTG 1 cut(s) 106
Sse9I AATT 5 cut(s) 139, 170, 353, 487, 548
SsiI CCGC 2 cut(s) 5, 484
SspMI CTAG 2 cut(s) 423, 717
SstI GAGCTC 1 cut(s) 78
StyD4I CCNGG 2 cut(s) 114, 174
StyI CCWWGG 1 cut(s) 519
TaaI ACNGT 1 cut(s) 458
TaiI ACGT 1 cut(s) 440
TaqI TCGA 2 cut(s) 540, 643
TasI AATT 5 cut(s) 139, 170, 353, 487, 548
TatI WGTACW 1 cut(s) 476
TscAI CASTG 3 cut(s) 31, 108, 256
TseFI GTSAC 2 cut(s) 26, 616
TseI GCWGC 1 cut(s) 605
Tsp45I GTSAC 2 cut(s) 26, 616
TspDTI ATGAA 4 cut(s) 342, 387, 432, 515
TspRI CASTG 3 cut(s) 31, 108, 256
XapI RAATTY 2 cut(s) 139, 170
XmnI GAANNNNTTC 2 cut(s) 170, 579
XspI CTAG 2 cut(s) 423, 717
ZrmI AGTACT 1 cut(s) 478
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.