FvH4_6g35380

complex I intermediate-associated protein 30

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0005050
Physical Location & Seq
Reverse (-)
27928453 .. 27931392
2940 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g35380.t5

Sequence Viewer

Length: 666 bp
ATGTCGAGGTTTCGATCACTTCTGCAAGCAACCAAGAAAGCTCTCACATGGAATGTCGAAGACCTGGTGCCTCCCTCTGAGAGATTTATATTTAATTTCAAATCCAAGGATGAGTTGAAGAAGTGGCATTTATATTCGGATTCCGAATATGGAGGGTTGTCCTCAGCATCTTTAGAGATCTCAGATACCGGAAATGGATCCAATGGAATTTTCTCTGGTAACCTTTCTTCGGATTTTGCTGAGGGTTCAAAATGGAAGATGAACAGGAGTGGCTTTTGTGGAATGCGGTCCAAAAAGTTCAATGGCTTCATTGATTTAGAGCCATATGATACCATATCACTAAAAGTTAAAGGAGATGGAAGATCCTACATATCTACGATTTATACAGAAAACTGGGTAAATTCCCCTGGACAAGAAGAAGATAATATATGGCAGGCTTTTGTTTTTGTACCCAAAGACAACTGGTACATTGCAAAGATCCCTCTTTCTCGTTATCTGCCAACATGGAGAGGGAATGTTATAGATGCAGAAATGGAAATGAATCCTGCTCGCGTTGTTGGTATGTCTCTCTCAGTGAATGCAGAAGGTGGTGTCCCGGGTGCTAGATCTGGTCCAGGTGATTTCAAGCTGGAAATTGATTGGATAAAGGCTTTAAGAACAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
CIA30

Protein Analysis

222

Amino Acids

24.88

Weight (kDa)

6.0

Isoelectric Point (pI)

39.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CIA30 PF08547 31 - 158 3.1e-30 Complex I intermediate-associated protein 30 (CIA30)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 67
AccII CGCG 1 cut(s) 552
AciI CCGC 1 cut(s) 286
AclWI GGATC 4 cut(s) 192, 205, 357, 472
AcsI RAATTY 2 cut(s) 207, 400
AfaI GTAC 2 cut(s) 450, 467
AfiI CCNNNNNNNGG 1 cut(s) 229
AgsI TTSAA 5 cut(s) 100, 118, 249, 301, 625
AjnI CCWGG 3 cut(s) 63, 406, 613
AjuI GAANNNNNNNTTGG 2 cut(s) 194, 226
AluBI AGCT 2 cut(s) 41, 628
AluI AGCT 2 cut(s) 41, 628
Alw26I GTCTC 1 cut(s) 570
AlwI GGATC 4 cut(s) 192, 205, 357, 472
Ama87I CYCGRG 1 cut(s) 595
ApoI RAATTY 2 cut(s) 207, 400
AspS9I GGNCC 2 cut(s) 288, 611
AsuC2I CCSGG 2 cut(s) 596, 597
AsuHPI GGTGA 1 cut(s) 629
AvaI CYCGRG 1 cut(s) 595
AvaII GGWCC 2 cut(s) 288, 611
BamHI GGATCC 1 cut(s) 197
BanI GGYRCC 1 cut(s) 67
BarI GAAGNNNNNNTAC 2 cut(s) 211, 243
BbsI GAAGAC 1 cut(s) 66
BbvCI CCTCAGC 2 cut(s) 163, 240
BccI CCATC 1 cut(s) 350
BciT130I CCWGG 3 cut(s) 65, 408, 615
BcnI CCSGG 2 cut(s) 596, 597
BcoDI GTCTC 1 cut(s) 570
BfaI CTAG 1 cut(s) 603
BglII AGATCT 2 cut(s) 177, 605
Bme1390I CCNGG 5 cut(s) 65, 408, 596, 597, 615
Bme18I GGWCC 2 cut(s) 288, 611
BmeT110I CYCGRG 1 cut(s) 595
BmgT120I GGNCC 2 cut(s) 288, 611
BmiI GGNNCC 2 cut(s) 69, 199
BmrFI CCNGG 5 cut(s) 65, 408, 596, 597, 615
BmrI ACTGGG 1 cut(s) 403
BmsI GCATC 2 cut(s) 176, 514
BmuI ACTGGG 1 cut(s) 403
BpiI GAAGAC 1 cut(s) 66
Bpu10I CCTNAGC 2 cut(s) 163, 240
BpuMI CCSGG 2 cut(s) 596, 597
BsaJI CCNNGG 3 cut(s) 105, 406, 595
BsaWI WCCGGW 1 cut(s) 188
Bsc4I CCNNNNNNNGG 1 cut(s) 229
Bse1I ACTGG 2 cut(s) 398, 467
Bse3DI GCAATG 1 cut(s) 468
BseBI CCWGG 3 cut(s) 65, 408, 615
BseDI CCNNGG 3 cut(s) 105, 406, 595
BseGI GGATG 1 cut(s) 115
BseLI CCNNNNNNNGG 1 cut(s) 229
BseMI GCAATG 1 cut(s) 468
BseMII CTCAG 5 cut(s) 69, 177, 195, 231, 585
BseNI ACTGG 2 cut(s) 398, 467
Bsh1236I CGCG 1 cut(s) 552
BshNI GGYRCC 1 cut(s) 67
BsiHKCI CYCGRG 1 cut(s) 595
BsiSI CCGG 2 cut(s) 189, 596
BslFI GGGAC 1 cut(s) 578
BslI CCNNNNNNNGG 1 cut(s) 229
BsmAI GTCTC 1 cut(s) 570
BsmFI GGGAC 1 cut(s) 578
BsmI GAATGC 2 cut(s) 288, 583
BsoBI CYCGRG 1 cut(s) 595
Bsp143I GATC 6 cut(s) 14, 177, 197, 362, 477, 605
BspACI CCGC 1 cut(s) 286
BspCNI CTCAG 5 cut(s) 70, 176, 194, 232, 584
BspFNI CGCG 1 cut(s) 552
BspLI GGNNCC 2 cut(s) 69, 199
BspPI GGATC 4 cut(s) 192, 205, 357, 472
BspT107I GGYRCC 1 cut(s) 67
BsrDI GCAATG 1 cut(s) 468
BsrI ACTGG 2 cut(s) 398, 467
BssECI CCNNGG 3 cut(s) 105, 406, 595
BssMI GATC 6 cut(s) 14, 177, 197, 362, 477, 605
BssT1I CCWWGG 1 cut(s) 105
Bst2UI CCWGG 3 cut(s) 65, 408, 615
BstC8I GCNNGC 3 cut(s) 27, 435, 550
BstDEI CTNAG 5 cut(s) 78, 163, 181, 240, 571
BstEII GGTNACC 1 cut(s) 218
BstF5I GGATG 1 cut(s) 115
BstFNI CGCG 1 cut(s) 552
BstKTI GATC 6 cut(s) 17, 180, 200, 365, 480, 608
BstMAI GTCTC 1 cut(s) 570
BstMBI GATC 6 cut(s) 14, 177, 197, 362, 477, 605
BstNI CCWGG 3 cut(s) 65, 408, 615
BstPI GGTNACC 1 cut(s) 218
BstSCI CCNGG 5 cut(s) 63, 406, 594, 595, 613
BstUI CGCG 1 cut(s) 552
BstV2I GAAGAC 1 cut(s) 66
BstX2I RGATCY 5 cut(s) 177, 197, 362, 477, 605
BstYI RGATCY 5 cut(s) 177, 197, 362, 477, 605
BtsCI GGATG 1 cut(s) 115
BtsIMutI CAGTG 1 cut(s) 579
Cac8I GCNNGC 3 cut(s) 27, 435, 550
Cfr13I GGNCC 2 cut(s) 288, 611
Cfr9I CCCGGG 1 cut(s) 595
CsiI ACCWGGT 1 cut(s) 63
Csp6I GTAC 2 cut(s) 449, 466
CviAII CATG 2 cut(s) 48, 504
CviJI RGCY 7 cut(s) 41, 273, 306, 322, 437, 628, 650
CviKI_1 RGCY 7 cut(s) 41, 273, 306, 322, 437, 628, 650
CviQI GTAC 2 cut(s) 449, 466
DdeI CTNAG 5 cut(s) 78, 163, 181, 240, 571
DpnI GATC 6 cut(s) 16, 179, 199, 364, 479, 607
DpnII GATC 6 cut(s) 14, 177, 197, 362, 477, 605
Eco130I CCWWGG 1 cut(s) 105
Eco47I GGWCC 2 cut(s) 288, 611
Eco88I CYCGRG 1 cut(s) 595
Eco91I GGTNACC 1 cut(s) 218
EcoO65I GGTNACC 1 cut(s) 218
EcoRII CCWGG 3 cut(s) 63, 406, 613
EcoT14I CCWWGG 1 cut(s) 105
ErhI CCWWGG 1 cut(s) 105
FaeI CATG 2 cut(s) 51, 507
FaqI GGGAC 1 cut(s) 578
FatI CATG 2 cut(s) 47, 503
FauNDI CATATG 1 cut(s) 325
FokI GGATG 1 cut(s) 122
FspBI CTAG 1 cut(s) 603
HapII CCGG 2 cut(s) 189, 596
Hin1II CATG 2 cut(s) 51, 507
HinfI GANTC 2 cut(s) 140, 541
HpaII CCGG 2 cut(s) 189, 596
HphI GGTGA 1 cut(s) 629
Hpy188I TCNGA 5 cut(s) 79, 139, 145, 184, 232
HpyAV CCTTC 1 cut(s) 578
HpyCH4V TGCA 4 cut(s) 25, 473, 527, 581
HpyF3I CTNAG 5 cut(s) 78, 163, 181, 240, 571
Hsp92II CATG 2 cut(s) 51, 507
Kzo9I GATC 6 cut(s) 14, 177, 197, 362, 477, 605
LweI GCATC 2 cut(s) 176, 514
MabI ACCWGGT 1 cut(s) 63
MaeI CTAG 1 cut(s) 603
MaeIII GTNAC 1 cut(s) 218
MalI GATC 6 cut(s) 16, 179, 199, 364, 479, 607
MboI GATC 6 cut(s) 14, 177, 197, 362, 477, 605
MboII GAAGA 7 cut(s) 71, 130, 219, 268, 372, 428, 431
MflI RGATCY 5 cut(s) 177, 197, 362, 477, 605
MluCI AATT 4 cut(s) 94, 207, 400, 633
MnlI CCTC 7 cut(s) 81, 85, 146, 172, 235, 492, 503
MseI TTAA 3 cut(s) 93, 348, 653
MspI CCGG 2 cut(s) 189, 596
MspR9I CCNGG 5 cut(s) 65, 408, 596, 597, 615
Mva1269I GAATGC 2 cut(s) 288, 583
MvaI CCWGG 3 cut(s) 65, 408, 615
MvnI CGCG 1 cut(s) 552
NciI CCSGG 2 cut(s) 596, 597
NdeI CATATG 1 cut(s) 325
NdeII GATC 6 cut(s) 14, 177, 197, 362, 477, 605
NlaIII CATG 2 cut(s) 51, 507
NlaIV GGNNCC 2 cut(s) 69, 199
PctI GAATGC 2 cut(s) 288, 583
PfeI GAWTC 2 cut(s) 140, 541
Psp6I CCWGG 3 cut(s) 63, 406, 613
PspEI GGTNACC 1 cut(s) 218
PspGI CCWGG 3 cut(s) 63, 406, 613
PspN4I GGNNCC 2 cut(s) 69, 199
PspPI GGNCC 2 cut(s) 288, 611
PsuI RGATCY 5 cut(s) 177, 197, 362, 477, 605
RsaI GTAC 2 cut(s) 450, 467
RsaNI GTAC 2 cut(s) 449, 466
SaqAI TTAA 3 cut(s) 93, 348, 653
Sau3AI GATC 6 cut(s) 14, 177, 197, 362, 477, 605
Sau96I GGNCC 2 cut(s) 288, 611
ScrFI CCNGG 5 cut(s) 65, 408, 596, 597, 615
SetI ASST 7 cut(s) 11, 43, 66, 225, 589, 619, 630
SexAI ACCWGGT 1 cut(s) 63
SfaNI GCATC 2 cut(s) 176, 514
SinI GGWCC 2 cut(s) 288, 611
SmaI CCCGGG 1 cut(s) 597
Sse9I AATT 4 cut(s) 94, 207, 400, 633
SsiI CCGC 1 cut(s) 286
SspMI CTAG 1 cut(s) 603
StyD4I CCNGG 5 cut(s) 63, 406, 594, 595, 613
StyI CCWWGG 1 cut(s) 105
TaqI TCGA 3 cut(s) 5, 13, 57
TasI AATT 4 cut(s) 94, 207, 400, 633
TfiI GAWTC 2 cut(s) 140, 541
Tru1I TTAA 3 cut(s) 93, 348, 653
Tru9I TTAA 3 cut(s) 93, 348, 653
TscAI CASTG 1 cut(s) 579
TspDTI ATGAA 3 cut(s) 275, 298, 554
TspMI CCCGGG 1 cut(s) 595
TspRI CASTG 1 cut(s) 579
VpaK11BI GGWCC 2 cut(s) 288, 611
XapI RAATTY 2 cut(s) 207, 400
XmaI CCCGGG 1 cut(s) 595
XspI CTAG 1 cut(s) 603
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.