RchiOBHm_Chr2g0146911

complex I intermediate-associated protein 30

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0005050
Physical Location & Seq
Reverse (-)
64526209 .. 64528965
2757 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51658

Sequence Viewer

Length: 666 bp
ATGTCCAGGTTTCGAGCACTGTTGCAAGCAACCAAGAAAGCTCTCACATGGAATGTCGAAGACCTGGTGCCTCCCAGTGAGAGATATATATTTAATTTCGGTTCAAAGGAAGAGCTGAAGAAGTGGCATTTATATTCTGATTCTGAATATGGAGGGTTGTCCTCAGCATCTTTAGAGATATCAGATACTGGAAATGGACTCAATGGAATTTTCTCGGGTAACCTTTCTTCTGATTTTGCTGAGGGTTCAAAGTGGAAGATGAACAGGAGTGGCTTTTGTGGAATGCGGTCCAAAAAGTTTAATGGCTTCATTGATTTGGATGCATATGATACCATATCACTAAAAGTTAAAGGAGACGGAAGATCCTACATATCTACGATTTATACAGAAAACTGGGTAAATTCCCCTGGACAAGATGAAGATAATTCATGGCAGGCTTTTGTTTTTGTACCAAAAGACAACTGGTACATTGCAAAGATTCCTCTTTCTCGTTATCTACCAACATGGAGAGGGAGTGTTATAGACGCAGAGATGGAAATGAATCCTGCCCGCGTTGTTGGTATGTCTCTCTCAGTGAATGCAGAAGGTGGTGTCCCGGGTGCTAGATCTGGTCCGGGTGATTTCAAGCTTGAAATTGATTGGATAAAGGCTTTAAGAACAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
CIA30

Protein Analysis

221

Amino Acids

24.74

Weight (kDa)

5.63

Isoelectric Point (pI)

35.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CIA30 PF08547 31 - 212 3e-43 Complex I intermediate-associated protein 30 (CIA30)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 67
AccII CGCG 1 cut(s) 552
AciI CCGC 2 cut(s) 286, 550
AclWI GGATC 1 cut(s) 357
AcsI RAATTY 2 cut(s) 207, 400
AcuI CTGAAG 1 cut(s) 137
AfaI GTAC 2 cut(s) 450, 467
AgsI TTSAA 4 cut(s) 105, 249, 625, 632
AjnI CCWGG 3 cut(s) 5, 63, 406
AluBI AGCT 3 cut(s) 41, 115, 628
AluI AGCT 3 cut(s) 41, 115, 628
Alw21I GWGCWC 1 cut(s) 19
Alw26I GTCTC 2 cut(s) 348, 570
AlwI GGATC 1 cut(s) 357
AlwNI CAGNNNCTG 1 cut(s) 188
Ama87I CYCGRG 2 cut(s) 214, 595
ApoI RAATTY 2 cut(s) 207, 400
AspS9I GGNCC 2 cut(s) 288, 611
AsuC2I CCSGG 3 cut(s) 596, 597, 615
AsuHPI GGTGA 1 cut(s) 629
AvaI CYCGRG 2 cut(s) 214, 595
AvaII GGWCC 2 cut(s) 288, 611
BanI GGYRCC 1 cut(s) 67
BarI GAAGNNNNNNTAC 2 cut(s) 211, 243
BbsI GAAGAC 1 cut(s) 66
Bbv12I GWGCWC 1 cut(s) 19
BbvCI CCTCAGC 2 cut(s) 163, 240
BccI CCATC 1 cut(s) 526
BciT130I CCWGG 3 cut(s) 7, 65, 408
BcnI CCSGG 3 cut(s) 596, 597, 615
BcoDI GTCTC 2 cut(s) 348, 570
BfaI CTAG 1 cut(s) 603
BglII AGATCT 1 cut(s) 605
Bme1390I CCNGG 6 cut(s) 7, 65, 408, 596, 597, 615
Bme18I GGWCC 2 cut(s) 288, 611
BmeT110I CYCGRG 2 cut(s) 214, 595
BmgT120I GGNCC 2 cut(s) 288, 611
BmiI GGNNCC 1 cut(s) 69
BmrFI CCNGG 6 cut(s) 7, 65, 408, 596, 597, 615
BmrI ACTGGG 2 cut(s) 69, 403
BmsI GCATC 2 cut(s) 176, 310
BmuI ACTGGG 2 cut(s) 69, 403
BpiI GAAGAC 1 cut(s) 66
Bpu10I CCTNAGC 2 cut(s) 163, 240
BpuMI CCSGG 3 cut(s) 596, 597, 615
BsaJI CCNNGG 2 cut(s) 406, 595
BsaXI ACNNNNNCTCC 2 cut(s) 499, 529
Bse1I ACTGG 4 cut(s) 75, 193, 398, 467
Bse3DI GCAATG 1 cut(s) 468
BseBI CCWGG 3 cut(s) 7, 65, 408
BseDI CCNNGG 2 cut(s) 406, 595
BseGI GGATG 1 cut(s) 325
BseMI GCAATG 1 cut(s) 468
BseMII CTCAG 3 cut(s) 177, 231, 585
BseNI ACTGG 4 cut(s) 75, 193, 398, 467
Bsh1236I CGCG 1 cut(s) 552
BshNI GGYRCC 1 cut(s) 67
BsiHKAI GWGCWC 1 cut(s) 19
BsiHKCI CYCGRG 2 cut(s) 214, 595
BsiSI CCGG 2 cut(s) 596, 614
BslFI GGGAC 1 cut(s) 578
BsmAI GTCTC 2 cut(s) 348, 570
BsmBI CGTCTC 1 cut(s) 348
BsmFI GGGAC 1 cut(s) 578
BsmI GAATGC 2 cut(s) 288, 583
BsoBI CYCGRG 2 cut(s) 214, 595
Bsp1286I GDGCHC 1 cut(s) 19
Bsp143I GATC 2 cut(s) 362, 605
BspACI CCGC 2 cut(s) 286, 550
BspCNI CTCAG 3 cut(s) 176, 232, 584
BspFNI CGCG 1 cut(s) 552
BspLI GGNNCC 1 cut(s) 69
BspPI GGATC 1 cut(s) 357
BspQI GCTCTTC 1 cut(s) 105
BspT107I GGYRCC 1 cut(s) 67
BsrDI GCAATG 1 cut(s) 468
BsrI ACTGG 4 cut(s) 75, 193, 398, 467
BssECI CCNNGG 2 cut(s) 406, 595
BssMI GATC 2 cut(s) 362, 605
Bst2UI CCWGG 3 cut(s) 7, 65, 408
Bst4CI ACNGT 1 cut(s) 21
Bst6I CTCTTC 1 cut(s) 105
BstC8I GCNNGC 3 cut(s) 27, 435, 550
BstDEI CTNAG 3 cut(s) 163, 240, 571
BstEII GGTNACC 1 cut(s) 218
BstF5I GGATG 1 cut(s) 325
BstFNI CGCG 1 cut(s) 552
BstKTI GATC 2 cut(s) 365, 608
BstMAI GTCTC 2 cut(s) 348, 570
BstMBI GATC 2 cut(s) 362, 605
BstNI CCWGG 3 cut(s) 7, 65, 408
BstPI GGTNACC 1 cut(s) 218
BstSCI CCNGG 6 cut(s) 5, 63, 406, 594, 595, 613
BstUI CGCG 1 cut(s) 552
BstV2I GAAGAC 1 cut(s) 66
BstX2I RGATCY 2 cut(s) 362, 605
BstYI RGATCY 2 cut(s) 362, 605
BtsCI GGATG 1 cut(s) 325
BtsIMutI CAGTG 3 cut(s) 17, 82, 579
Cac8I GCNNGC 3 cut(s) 27, 435, 550
CaiI CAGNNNCTG 1 cut(s) 188
Cfr13I GGNCC 2 cut(s) 288, 611
Cfr9I CCCGGG 1 cut(s) 595
CseI GACGC 1 cut(s) 533
CsiI ACCWGGT 1 cut(s) 63
Csp6I GTAC 2 cut(s) 449, 466
CviAII CATG 3 cut(s) 48, 429, 504
CviJI RGCY 7 cut(s) 41, 115, 273, 306, 437, 628, 650
CviKI_1 RGCY 7 cut(s) 41, 115, 273, 306, 437, 628, 650
CviQI GTAC 2 cut(s) 449, 466
DdeI CTNAG 3 cut(s) 163, 240, 571
DpnI GATC 2 cut(s) 364, 607
DpnII GATC 2 cut(s) 362, 605
Eam1104I CTCTTC 1 cut(s) 105
EarI CTCTTC 1 cut(s) 105
Eco32I GATATC 1 cut(s) 180
Eco47I GGWCC 2 cut(s) 288, 611
Eco57I CTGAAG 1 cut(s) 137
Eco88I CYCGRG 2 cut(s) 214, 595
Eco91I GGTNACC 1 cut(s) 218
EcoO65I GGTNACC 1 cut(s) 218
EcoRII CCWGG 3 cut(s) 5, 63, 406
EcoRV GATATC 1 cut(s) 180
EcoT22I ATGCAT 1 cut(s) 325
Esp3I CGTCTC 1 cut(s) 348
FaeI CATG 3 cut(s) 51, 432, 507
FaqI GGGAC 1 cut(s) 578
FatI CATG 3 cut(s) 47, 428, 503
FauI CCCGC 1 cut(s) 557
FauNDI CATATG 1 cut(s) 325
FokI GGATG 1 cut(s) 332
FspBI CTAG 1 cut(s) 603
HapII CCGG 2 cut(s) 596, 614
HgaI GACGC 1 cut(s) 533
Hin1II CATG 3 cut(s) 51, 432, 507
HindIII AAGCTT 1 cut(s) 626
HinfI GANTC 4 cut(s) 140, 198, 478, 541
HpaII CCGG 2 cut(s) 596, 614
HphI GGTGA 1 cut(s) 629
Hpy188I TCNGA 4 cut(s) 139, 145, 184, 232
HpyAV CCTTC 1 cut(s) 578
HpyCH4III ACNGT 1 cut(s) 21
HpyCH4V TGCA 4 cut(s) 25, 323, 473, 581
HpyF3I CTNAG 3 cut(s) 163, 240, 571
Hsp92II CATG 3 cut(s) 51, 432, 507
Kzo9I GATC 2 cut(s) 362, 605
LguI GCTCTTC 1 cut(s) 105
LweI GCATC 2 cut(s) 176, 310
MabI ACCWGGT 1 cut(s) 63
MaeI CTAG 1 cut(s) 603
MaeIII GTNAC 1 cut(s) 218
MalI GATC 2 cut(s) 364, 607
MboI GATC 2 cut(s) 362, 605
MboII GAAGA 7 cut(s) 71, 122, 130, 219, 268, 372, 431
MflI RGATCY 2 cut(s) 362, 605
MhlI GDGCHC 1 cut(s) 19
MluCI AATT 5 cut(s) 94, 207, 400, 424, 633
MlyI GAGTC 1 cut(s) 192
MnlI CCTC 6 cut(s) 81, 146, 172, 235, 492, 503
Mph1103I ATGCAT 1 cut(s) 325
MseI TTAA 4 cut(s) 93, 300, 348, 653
MspI CCGG 2 cut(s) 596, 614
MspR9I CCNGG 6 cut(s) 7, 65, 408, 596, 597, 615
Mva1269I GAATGC 2 cut(s) 288, 583
MvaI CCWGG 3 cut(s) 7, 65, 408
MvnI CGCG 1 cut(s) 552
NciI CCSGG 3 cut(s) 596, 597, 615
NdeI CATATG 1 cut(s) 325
NdeII GATC 2 cut(s) 362, 605
NlaIII CATG 3 cut(s) 51, 432, 507
NlaIV GGNNCC 1 cut(s) 69
NsiI ATGCAT 1 cut(s) 325
PciSI GCTCTTC 1 cut(s) 105
PctI GAATGC 2 cut(s) 288, 583
PfeI GAWTC 3 cut(s) 140, 478, 541
PleI GAGTC 1 cut(s) 192
PpsI GAGTC 1 cut(s) 192
Psp6I CCWGG 3 cut(s) 5, 63, 406
PspEI GGTNACC 1 cut(s) 218
PspGI CCWGG 3 cut(s) 5, 63, 406
PspN4I GGNNCC 1 cut(s) 69
PspPI GGNCC 2 cut(s) 288, 611
PstNI CAGNNNCTG 1 cut(s) 188
PsuI RGATCY 2 cut(s) 362, 605
RsaI GTAC 2 cut(s) 450, 467
RsaNI GTAC 2 cut(s) 449, 466
SapI GCTCTTC 1 cut(s) 105
SaqAI TTAA 4 cut(s) 93, 300, 348, 653
Sau3AI GATC 2 cut(s) 362, 605
Sau96I GGNCC 2 cut(s) 288, 611
SchI GAGTC 1 cut(s) 192
ScrFI CCNGG 6 cut(s) 7, 65, 408, 596, 597, 615
SduI GDGCHC 1 cut(s) 19
SetI ASST 7 cut(s) 11, 43, 66, 117, 225, 589, 630
SexAI ACCWGGT 1 cut(s) 63
SfaNI GCATC 2 cut(s) 176, 310
SinI GGWCC 2 cut(s) 288, 611
SmaI CCCGGG 1 cut(s) 597
Sse9I AATT 5 cut(s) 94, 207, 400, 424, 633
SsiI CCGC 2 cut(s) 286, 550
SspMI CTAG 1 cut(s) 603
StyD4I CCNGG 6 cut(s) 5, 63, 406, 594, 595, 613
TaaI ACNGT 1 cut(s) 21
TaqI TCGA 2 cut(s) 13, 57
TasI AATT 5 cut(s) 94, 207, 400, 424, 633
TfiI GAWTC 3 cut(s) 140, 478, 541
Tru1I TTAA 4 cut(s) 93, 300, 348, 653
Tru9I TTAA 4 cut(s) 93, 300, 348, 653
TscAI CASTG 3 cut(s) 24, 82, 579
TspDTI ATGAA 5 cut(s) 275, 298, 417, 432, 554
TspGWI ACGGA 1 cut(s) 372
TspMI CCCGGG 1 cut(s) 595
TspRI CASTG 3 cut(s) 24, 82, 579
VpaK11BI GGWCC 2 cut(s) 288, 611
XapI RAATTY 2 cut(s) 207, 400
XcmI CCANNNNNNNNNTGG 1 cut(s) 459
XmaI CCCGGG 1 cut(s) 595
XspI CTAG 1 cut(s) 603
Zsp2I ATGCAT 1 cut(s) 325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.