Rh2BG466500

complex I intermediate-associated protein 30

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0005050
Physical Location & Seq
Reverse (-)
65883691 .. 65886514
2824 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG466500.1

Sequence Viewer

Length: 834 bp
ATGTCCAGGTTTCGAGCACTGTTGCAAGCAACCAAGAAAGGTATTATATTCCTCTTTGTGTTACTTGAGACATTCTACGAGCATTGCTTAAGTCCTGTTCAGTGGTTCAAGTTTAGAGGAGATGTTTGGCTTTTTTCACTTATGATATATGAACTTAAATATTTGTATCGTCACTATTCTGTTTCTTGTGCATTGAATTGCAATTTAGCTCTCACATGGAATGTCGAAGACCTGGTGCCTCCCAGTGAGAGATATATATTTAATTTCGGTTCAAAGGAAGAGCTGAAGAAGTGGCATTTATATTCTGATTCTGAATATGGAGGGTTGTCCTCAGCATCTTTAGAGATATCAGATACTGGAAATGGACTCAATGGAATTTTCTCGGGTAACCTTTCTTCTGATTTTGCTGAGGGTTCAAAGTGGAAGATGAACAGGAGTGGCTTTTGTGGAATGCGGTCCAAAAAGTTTAATGGCTTCATTGATTTGGATGCATATGATACCATATCACTAAAAGTTAAAGGAGACGGAAGATCCTACATATCTACGATTTATACAGAAAACTGGGTAAATTCCCCTGGACAAGATGAAGATAATTCATGGCAGGCTTTTGTTTTTGTACCAAAAGACAACTGGTACATTGCAAAGATTCCTCTTTCTCGTTATCTACCAACATGGAGAGGGAGTGTTATAGACGCAGAGATGGAAATGAATCCTGCCCGCGTTGTTGGTATGTCTCTCTCAGTGAATGCAGAAGGTGGTGTCCCGGGTGCTAGATCTGGTCCGGGTGATTTCAAGCTTGAAATTGATTGGATAAAGGCTTTAAGAACAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
CIA30

Protein Analysis

277

Amino Acids

31.55

Weight (kDa)

6.02

Isoelectric Point (pI)

35.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CIA30 PF08547 87 - 268 6.2e-43 Complex I intermediate-associated protein 30 (CIA30)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 235
AccII CGCG 1 cut(s) 720
AciI CCGC 2 cut(s) 454, 718
AclWI GGATC 1 cut(s) 525
AcsI RAATTY 2 cut(s) 375, 568
AcuI CTGAAG 1 cut(s) 305
AfaI GTAC 2 cut(s) 618, 635
AflII CTTAAG 1 cut(s) 88
AgsI TTSAA 6 cut(s) 109, 196, 273, 417, 793, 800
AjnI CCWGG 3 cut(s) 5, 231, 574
AluBI AGCT 3 cut(s) 209, 283, 796
AluI AGCT 3 cut(s) 209, 283, 796
Alw21I GWGCWC 1 cut(s) 19
Alw26I GTCTC 3 cut(s) 62, 516, 738
AlwI GGATC 1 cut(s) 525
AlwNI CAGNNNCTG 1 cut(s) 356
Ama87I CYCGRG 2 cut(s) 382, 763
ApoI RAATTY 2 cut(s) 375, 568
AspS9I GGNCC 2 cut(s) 456, 779
AsuC2I CCSGG 3 cut(s) 764, 765, 783
AsuHPI GGTGA 1 cut(s) 797
AvaI CYCGRG 2 cut(s) 382, 763
AvaII GGWCC 2 cut(s) 456, 779
BanI GGYRCC 1 cut(s) 235
BarI GAAGNNNNNNTAC 2 cut(s) 379, 411
BbsI GAAGAC 1 cut(s) 234
Bbv12I GWGCWC 1 cut(s) 19
BbvCI CCTCAGC 2 cut(s) 331, 408
BccI CCATC 1 cut(s) 694
BciT130I CCWGG 3 cut(s) 7, 233, 576
BcnI CCSGG 3 cut(s) 764, 765, 783
BcoDI GTCTC 3 cut(s) 62, 516, 738
BfaI CTAG 1 cut(s) 771
BfrI CTTAAG 1 cut(s) 88
BglII AGATCT 1 cut(s) 773
Bme1390I CCNGG 6 cut(s) 7, 233, 576, 764, 765, 783
Bme18I GGWCC 2 cut(s) 456, 779
BmeT110I CYCGRG 2 cut(s) 382, 763
BmgT120I GGNCC 2 cut(s) 456, 779
BmiI GGNNCC 1 cut(s) 237
BmrFI CCNGG 6 cut(s) 7, 233, 576, 764, 765, 783
BmrI ACTGGG 2 cut(s) 237, 571
BmsI GCATC 2 cut(s) 344, 478
BmuI ACTGGG 2 cut(s) 237, 571
BpiI GAAGAC 1 cut(s) 234
Bpu10I CCTNAGC 2 cut(s) 331, 408
BpuEI CTTGAG 1 cut(s) 86
BpuMI CCSGG 3 cut(s) 764, 765, 783
BsaJI CCNNGG 2 cut(s) 574, 763
BsaXI ACNNNNNCTCC 2 cut(s) 667, 697
Bse1I ACTGG 4 cut(s) 243, 361, 566, 635
Bse3DI GCAATG 2 cut(s) 82, 636
BseBI CCWGG 3 cut(s) 7, 233, 576
BseDI CCNNGG 2 cut(s) 574, 763
BseGI GGATG 1 cut(s) 493
BseMI GCAATG 2 cut(s) 82, 636
BseMII CTCAG 3 cut(s) 345, 399, 753
BseNI ACTGG 4 cut(s) 243, 361, 566, 635
BseRI GAGGAG 1 cut(s) 132
Bsh1236I CGCG 1 cut(s) 720
BshNI GGYRCC 1 cut(s) 235
BsiHKAI GWGCWC 1 cut(s) 19
BsiHKCI CYCGRG 2 cut(s) 382, 763
BsiSI CCGG 2 cut(s) 764, 782
BslFI GGGAC 1 cut(s) 746
BsmAI GTCTC 3 cut(s) 62, 516, 738
BsmBI CGTCTC 1 cut(s) 516
BsmFI GGGAC 1 cut(s) 746
BsmI GAATGC 2 cut(s) 456, 751
BsoBI CYCGRG 2 cut(s) 382, 763
Bsp1286I GDGCHC 1 cut(s) 19
Bsp143I GATC 2 cut(s) 530, 773
BspACI CCGC 2 cut(s) 454, 718
BspCNI CTCAG 3 cut(s) 344, 400, 752
BspFNI CGCG 1 cut(s) 720
BspLI GGNNCC 1 cut(s) 237
BspPI GGATC 1 cut(s) 525
BspQI GCTCTTC 1 cut(s) 273
BspT107I GGYRCC 1 cut(s) 235
BspTI CTTAAG 1 cut(s) 88
BsrDI GCAATG 2 cut(s) 82, 636
BsrI ACTGG 4 cut(s) 243, 361, 566, 635
BssECI CCNNGG 2 cut(s) 574, 763
BssMI GATC 2 cut(s) 530, 773
Bst2UI CCWGG 3 cut(s) 7, 233, 576
Bst4CI ACNGT 1 cut(s) 21
Bst6I CTCTTC 1 cut(s) 273
BstAFI CTTAAG 1 cut(s) 88
BstC8I GCNNGC 3 cut(s) 27, 603, 718
BstDEI CTNAG 3 cut(s) 331, 408, 739
BstEII GGTNACC 1 cut(s) 386
BstF5I GGATG 1 cut(s) 493
BstFNI CGCG 1 cut(s) 720
BstKTI GATC 2 cut(s) 533, 776
BstMAI GTCTC 3 cut(s) 62, 516, 738
BstMBI GATC 2 cut(s) 530, 773
BstNI CCWGG 3 cut(s) 7, 233, 576
BstPI GGTNACC 1 cut(s) 386
BstSCI CCNGG 6 cut(s) 5, 231, 574, 762, 763, 781
BstUI CGCG 1 cut(s) 720
BstV2I GAAGAC 1 cut(s) 234
BstX2I RGATCY 2 cut(s) 530, 773
BstYI RGATCY 2 cut(s) 530, 773
BtsCI GGATG 1 cut(s) 493
BtsIMutI CAGTG 4 cut(s) 17, 107, 250, 747
Cac8I GCNNGC 3 cut(s) 27, 603, 718
CaiI CAGNNNCTG 1 cut(s) 356
Cfr13I GGNCC 2 cut(s) 456, 779
Cfr9I CCCGGG 1 cut(s) 763
CseI GACGC 1 cut(s) 701
CsiI ACCWGGT 1 cut(s) 231
Csp6I GTAC 2 cut(s) 617, 634
CviAII CATG 3 cut(s) 216, 597, 672
CviJI RGCY 8 cut(s) 130, 209, 283, 441, 474, 605, 796, 818
CviKI_1 RGCY 8 cut(s) 130, 209, 283, 441, 474, 605, 796, 818
CviQI GTAC 2 cut(s) 617, 634
DdeI CTNAG 3 cut(s) 331, 408, 739
DpnI GATC 2 cut(s) 532, 775
DpnII GATC 2 cut(s) 530, 773
Eam1104I CTCTTC 1 cut(s) 273
EarI CTCTTC 1 cut(s) 273
Eco32I GATATC 1 cut(s) 348
Eco47I GGWCC 2 cut(s) 456, 779
Eco57I CTGAAG 1 cut(s) 305
Eco88I CYCGRG 2 cut(s) 382, 763
Eco91I GGTNACC 1 cut(s) 386
EcoO65I GGTNACC 1 cut(s) 386
EcoRII CCWGG 3 cut(s) 5, 231, 574
EcoRV GATATC 1 cut(s) 348
EcoT22I ATGCAT 1 cut(s) 493
Esp3I CGTCTC 1 cut(s) 516
FaeI CATG 3 cut(s) 219, 600, 675
FaqI GGGAC 1 cut(s) 746
FatI CATG 3 cut(s) 215, 596, 671
FauI CCCGC 1 cut(s) 725
FauNDI CATATG 1 cut(s) 493
FokI GGATG 1 cut(s) 500
FspBI CTAG 1 cut(s) 771
HapII CCGG 2 cut(s) 764, 782
HgaI GACGC 1 cut(s) 701
Hin1II CATG 3 cut(s) 219, 600, 675
HindIII AAGCTT 1 cut(s) 794
HinfI GANTC 4 cut(s) 308, 366, 646, 709
HpaII CCGG 2 cut(s) 764, 782
HphI GGTGA 1 cut(s) 797
Hpy188I TCNGA 4 cut(s) 307, 313, 352, 400
HpyAV CCTTC 1 cut(s) 746
HpyCH4III ACNGT 1 cut(s) 21
HpyCH4V TGCA 6 cut(s) 25, 191, 201, 491, 641, 749
HpyF3I CTNAG 3 cut(s) 331, 408, 739
Hsp92II CATG 3 cut(s) 219, 600, 675
Kzo9I GATC 2 cut(s) 530, 773
LguI GCTCTTC 1 cut(s) 273
LweI GCATC 2 cut(s) 344, 478
MabI ACCWGGT 1 cut(s) 231
MaeI CTAG 1 cut(s) 771
MaeIII GTNAC 3 cut(s) 60, 170, 386
MalI GATC 2 cut(s) 532, 775
MboI GATC 2 cut(s) 530, 773
MboII GAAGA 7 cut(s) 239, 290, 298, 387, 436, 540, 599
MflI RGATCY 2 cut(s) 530, 773
MhlI GDGCHC 1 cut(s) 19
MluCI AATT 7 cut(s) 196, 202, 262, 375, 568, 592, 801
MlyI GAGTC 1 cut(s) 360
MnlI CCTC 8 cut(s) 62, 110, 249, 314, 340, 403, 660, 671
Mph1103I ATGCAT 1 cut(s) 493
MseI TTAA 6 cut(s) 89, 156, 261, 468, 516, 821
MspCI CTTAAG 1 cut(s) 88
MspI CCGG 2 cut(s) 764, 782
MspR9I CCNGG 6 cut(s) 7, 233, 576, 764, 765, 783
Mva1269I GAATGC 2 cut(s) 456, 751
MvaI CCWGG 3 cut(s) 7, 233, 576
MvnI CGCG 1 cut(s) 720
NciI CCSGG 3 cut(s) 764, 765, 783
NdeI CATATG 1 cut(s) 493
NdeII GATC 2 cut(s) 530, 773
NlaIII CATG 3 cut(s) 219, 600, 675
NlaIV GGNNCC 1 cut(s) 237
NmuCI GTSAC 1 cut(s) 170
NsiI ATGCAT 1 cut(s) 493
PciSI GCTCTTC 1 cut(s) 273
PctI GAATGC 2 cut(s) 456, 751
PfeI GAWTC 3 cut(s) 308, 646, 709
PleI GAGTC 1 cut(s) 360
PpsI GAGTC 1 cut(s) 360
Psp6I CCWGG 3 cut(s) 5, 231, 574
PspEI GGTNACC 1 cut(s) 386
PspGI CCWGG 3 cut(s) 5, 231, 574
PspN4I GGNNCC 1 cut(s) 237
PspPI GGNCC 2 cut(s) 456, 779
PstNI CAGNNNCTG 1 cut(s) 356
PsuI RGATCY 2 cut(s) 530, 773
RsaI GTAC 2 cut(s) 618, 635
RsaNI GTAC 2 cut(s) 617, 634
SapI GCTCTTC 1 cut(s) 273
SaqAI TTAA 6 cut(s) 89, 156, 261, 468, 516, 821
Sau3AI GATC 2 cut(s) 530, 773
Sau96I GGNCC 2 cut(s) 456, 779
SchI GAGTC 1 cut(s) 360
ScrFI CCNGG 6 cut(s) 7, 233, 576, 764, 765, 783
SduI GDGCHC 1 cut(s) 19
SetI ASST 8 cut(s) 11, 43, 211, 234, 285, 393, 757, 798
SexAI ACCWGGT 1 cut(s) 231
SfaNI GCATC 2 cut(s) 344, 478
SinI GGWCC 2 cut(s) 456, 779
SmaI CCCGGG 1 cut(s) 765
SmlI CTYRAG 2 cut(s) 65, 88
SmoI CTYRAG 2 cut(s) 65, 88
Sse9I AATT 7 cut(s) 196, 202, 262, 375, 568, 592, 801
SsiI CCGC 2 cut(s) 454, 718
SspI AATATT 1 cut(s) 161
SspMI CTAG 1 cut(s) 771
StyD4I CCNGG 6 cut(s) 5, 231, 574, 762, 763, 781
TaaI ACNGT 1 cut(s) 21
TaqI TCGA 2 cut(s) 13, 225
TasI AATT 7 cut(s) 196, 202, 262, 375, 568, 592, 801
TfiI GAWTC 3 cut(s) 308, 646, 709
Tru1I TTAA 6 cut(s) 89, 156, 261, 468, 516, 821
Tru9I TTAA 6 cut(s) 89, 156, 261, 468, 516, 821
TscAI CASTG 4 cut(s) 24, 107, 250, 747
TseFI GTSAC 1 cut(s) 170
Tsp45I GTSAC 1 cut(s) 170
TspDTI ATGAA 6 cut(s) 165, 443, 466, 585, 600, 722
TspGWI ACGGA 1 cut(s) 540
TspMI CCCGGG 1 cut(s) 763
TspRI CASTG 4 cut(s) 24, 107, 250, 747
Vha464I CTTAAG 1 cut(s) 88
VpaK11BI GGWCC 2 cut(s) 456, 779
XapI RAATTY 2 cut(s) 375, 568
XcmI CCANNNNNNNNNTGG 1 cut(s) 627
XmaI CCCGGG 1 cut(s) 763
XspI CTAG 1 cut(s) 771
Zsp2I ATGCAT 1 cut(s) 493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.