MD09G1176300.v1.1

complex I intermediate-associated protein 30

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0005050
Physical Location & Seq
Forward (+)
15075431 .. 15079517
4087 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1176300.v1.1.491

Sequence Viewer

Length: 675 bp
ATGTCCAGGTTTCGAGGAATGTTGCAAGCTTCCCTAAATGCAACCAAGAAAGCACTTACATGGAATGTTGAAGACCTGGTGCCTCCAACTGAGAGATTTATTTTCAATTTCAATTCGAAGGATGAGCTCAAGAAGTGGCATCTATATTCTGACTCTGAATACGGAGGGTTGTCCTCAGCATCTTTAGAAGTTCCAGATAATGAAAATGGGCCCACTGGAATTTTCTCGGGTAACCTTTCGTTGGATTTCATTGAGGGTGCAAAGCTGAAGATAAACAGGAGTGGCTTTTGTGGAATGCGTTCCAAAAAGTTTGATGGCTACATTGATTTGGACCCATATGATACCATAGCACTGAAAGTTAAAGGAGATGGAAGATGCTACATTTCCACGATCTATACAGAAAATTGGGTAAATTCACCTGGTCAAGAAGAAGATAATTCGTGGCAGGCTTTTGTTTTTGTACCGAAAGATAACTGGTACATTGCAAAGATTCCTCTTGCTCTGTATCTACCAACATGGAGAGGAAATGTTATCGATGCAGAAATGGAAATGAACCCTTCTCGCGTTGTTGGCATGTCTCTATCAGTCAATGCAGAAGGCGGTCTCCCAGGTGCTAGAACTGGAGCGGGTGATTTCAAGCTCGAAATTGATTGGATCAAGGCTTTACGAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
CIA30

Protein Analysis

225

Amino Acids

25.1

Weight (kDa)

5.2

Isoelectric Point (pI)

29.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CIA30 PF08547 35 - 216 5.8e-43 Complex I intermediate-associated protein 30 (CIA30)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 79
AccBSI CCGCTC 1 cut(s) 626
AccII CGCG 1 cut(s) 564
AciI CCGC 2 cut(s) 600, 626
AclWI GGATC 1 cut(s) 662
AcsI RAATTY 2 cut(s) 219, 412
AcuI CTGAAG 1 cut(s) 287
AfaI GTAC 2 cut(s) 462, 479
AfiI CCNNNNNNNGG 1 cut(s) 241
AgsI TTSAA 4 cut(s) 71, 106, 112, 637
AjnI CCWGG 4 cut(s) 5, 75, 418, 607
AluBI AGCT 4 cut(s) 29, 127, 265, 640
AluI AGCT 4 cut(s) 29, 127, 265, 640
Alw21I GWGCWC 1 cut(s) 129
Alw26I GTCTC 2 cut(s) 582, 608
AlwI GGATC 1 cut(s) 662
Ama87I CYCGRG 1 cut(s) 226
AoxI GGCC 1 cut(s) 209
ApaI GGGCCC 1 cut(s) 213
ApoI RAATTY 2 cut(s) 219, 412
Asp700I GAANNNNTTC 1 cut(s) 298
AspS9I GGNCC 3 cut(s) 209, 210, 331
AsuHPI GGTGA 2 cut(s) 408, 641
AsuII TTCGAA 1 cut(s) 116
AvaI CYCGRG 1 cut(s) 226
AvaII GGWCC 1 cut(s) 331
BaeGI GKGCMC 1 cut(s) 213
BanI GGYRCC 1 cut(s) 79
BanII GRGCYC 2 cut(s) 129, 213
BbsI GAAGAC 1 cut(s) 78
Bbv12I GWGCWC 1 cut(s) 129
BbvCI CCTCAGC 1 cut(s) 175
BccI CCATC 2 cut(s) 308, 362
BciT130I CCWGG 4 cut(s) 7, 77, 420, 609
BcoDI GTCTC 2 cut(s) 582, 608
BfaI CTAG 1 cut(s) 615
Bme1390I CCNGG 4 cut(s) 7, 77, 420, 609
Bme18I GGWCC 1 cut(s) 331
BmeT110I CYCGRG 1 cut(s) 226
BmgT120I GGNCC 3 cut(s) 209, 210, 331
BmiI GGNNCC 3 cut(s) 81, 211, 333
BmrFI CCNGG 4 cut(s) 7, 77, 420, 609
BmsI GCATC 4 cut(s) 148, 188, 365, 526
BpiI GAAGAC 1 cut(s) 78
BpmI CTGGAG 1 cut(s) 642
Bpu10I CCTNAGC 1 cut(s) 175
Bpu14I TTCGAA 1 cut(s) 116
BpuEI CTTGAG 1 cut(s) 113
Bsa29I ATCGAT 1 cut(s) 534
BsaI GGTCTC 1 cut(s) 608
BsaJI CCNNGG 1 cut(s) 607
Bsc4I CCNNNNNNNGG 1 cut(s) 241
Bse1I ACTGG 3 cut(s) 220, 479, 625
Bse3DI GCAATG 1 cut(s) 480
BseBI CCWGG 4 cut(s) 7, 77, 420, 609
BseCI ATCGAT 1 cut(s) 534
BseDI CCNNGG 1 cut(s) 607
BseGI GGATG 1 cut(s) 127
BseLI CCNNNNNNNGG 1 cut(s) 241
BseMI GCAATG 1 cut(s) 480
BseMII CTCAG 2 cut(s) 81, 189
BseNI ACTGG 3 cut(s) 220, 479, 625
BseSI GKGCMC 1 cut(s) 213
Bsh1236I CGCG 1 cut(s) 564
BshFI GGCC 1 cut(s) 211
BshNI GGYRCC 1 cut(s) 79
BshVI ATCGAT 1 cut(s) 534
BsiHKAI GWGCWC 1 cut(s) 129
BsiHKCI CYCGRG 1 cut(s) 226
BslI CCNNNNNNNGG 1 cut(s) 241
BsmAI GTCTC 2 cut(s) 582, 608
BsmI GAATGC 1 cut(s) 300
BsnI GGCC 1 cut(s) 211
Bso31I GGTCTC 1 cut(s) 608
BsoBI CYCGRG 1 cut(s) 226
Bsp119I TTCGAA 1 cut(s) 116
Bsp120I GGGCCC 1 cut(s) 209
Bsp1286I GDGCHC 2 cut(s) 129, 213
Bsp143I GATC 2 cut(s) 390, 654
BspACI CCGC 2 cut(s) 600, 626
BspANI GGCC 1 cut(s) 211
BspCNI CTCAG 2 cut(s) 82, 188
BspDI ATCGAT 1 cut(s) 534
BspFNI CGCG 1 cut(s) 564
BspLI GGNNCC 3 cut(s) 81, 211, 333
BspPI GGATC 1 cut(s) 662
BspT104I TTCGAA 1 cut(s) 116
BspT107I GGYRCC 1 cut(s) 79
BspTNI GGTCTC 1 cut(s) 608
BsrBI CCGCTC 1 cut(s) 626
BsrDI GCAATG 1 cut(s) 480
BsrI ACTGG 3 cut(s) 220, 479, 625
BssECI CCNNGG 1 cut(s) 607
BssMI GATC 2 cut(s) 390, 654
Bst2UI CCWGG 4 cut(s) 7, 77, 420, 609
BstBI TTCGAA 1 cut(s) 116
BstC8I GCNNGC 2 cut(s) 27, 447
BstDEI CTNAG 2 cut(s) 90, 175
BstEII GGTNACC 1 cut(s) 230
BstF5I GGATG 1 cut(s) 127
BstFNI CGCG 1 cut(s) 564
BstKTI GATC 2 cut(s) 393, 657
BstMAI GTCTC 2 cut(s) 582, 608
BstMBI GATC 2 cut(s) 390, 654
BstMWI GCNNNNNNNGC 1 cut(s) 570
BstNI CCWGG 4 cut(s) 7, 77, 420, 609
BstNSI RCATGY 1 cut(s) 577
BstPI GGTNACC 1 cut(s) 230
BstSCI CCNGG 4 cut(s) 5, 75, 418, 607
BstSLI GKGCMC 1 cut(s) 213
BstUI CGCG 1 cut(s) 564
BstV2I GAAGAC 1 cut(s) 78
Bsu15I ATCGAT 1 cut(s) 534
BsuRI GGCC 1 cut(s) 211
BsuTUI ATCGAT 1 cut(s) 534
BtsCI GGATG 1 cut(s) 127
BtsIMutI CAGTG 2 cut(s) 213, 350
Cac8I GCNNGC 2 cut(s) 27, 447
Cfr13I GGNCC 3 cut(s) 209, 210, 331
ClaI ATCGAT 1 cut(s) 534
CsiI ACCWGGT 2 cut(s) 75, 418
Csp6I GTAC 2 cut(s) 461, 478
CviAII CATG 3 cut(s) 60, 516, 574
CviJI RGCY 9 cut(s) 29, 127, 211, 265, 285, 318, 449, 640, 662
CviKI_1 RGCY 9 cut(s) 29, 127, 211, 265, 285, 318, 449, 640, 662
CviQI GTAC 2 cut(s) 461, 478
DdeI CTNAG 2 cut(s) 90, 175
DpnI GATC 2 cut(s) 392, 656
DpnII GATC 2 cut(s) 390, 654
Ecl136II GAGCTC 1 cut(s) 127
Eco24I GRGCYC 2 cut(s) 129, 213
Eco31I GGTCTC 1 cut(s) 608
Eco47I GGWCC 1 cut(s) 331
Eco53kI GAGCTC 1 cut(s) 127
Eco57I CTGAAG 1 cut(s) 287
Eco88I CYCGRG 1 cut(s) 226
Eco91I GGTNACC 1 cut(s) 230
EcoICRI GAGCTC 1 cut(s) 127
EcoO65I GGTNACC 1 cut(s) 230
EcoRII CCWGG 4 cut(s) 5, 75, 418, 607
EcoT38I GRGCYC 2 cut(s) 129, 213
FaeI CATG 3 cut(s) 63, 519, 577
FaiI YATR 9 cut(s) 61, 145, 337, 339, 347, 396, 517, 575, 673
FatI CATG 3 cut(s) 59, 515, 573
FauI CCCGC 1 cut(s) 619
FauNDI CATATG 1 cut(s) 337
FokI GGATG 1 cut(s) 134
FriOI GRGCYC 2 cut(s) 129, 213
FspBI CTAG 1 cut(s) 615
GsuI CTGGAG 1 cut(s) 642
HaeIII GGCC 1 cut(s) 211
Hin1II CATG 3 cut(s) 63, 519, 577
HindIII AAGCTT 1 cut(s) 27
HinfI GANTC 2 cut(s) 152, 490
HphI GGTGA 2 cut(s) 408, 641
Hpy188I TCNGA 2 cut(s) 151, 157
Hpy188III TCNNGA 3 cut(s) 130, 194, 425
HpyAV CCTTC 3 cut(s) 112, 567, 590
HpyCH4V TGCA 6 cut(s) 25, 41, 260, 485, 539, 593
HpyF10VI GCNNNNNNNGC 1 cut(s) 570
HpyF3I CTNAG 2 cut(s) 90, 175
Hsp92II CATG 3 cut(s) 63, 519, 577
Kzo9I GATC 2 cut(s) 390, 654
LmnI GCTCC 1 cut(s) 623
LweI GCATC 4 cut(s) 148, 188, 365, 526
MabI ACCWGGT 2 cut(s) 75, 418
MaeI CTAG 1 cut(s) 615
MaeIII GTNAC 1 cut(s) 230
MalI GATC 2 cut(s) 392, 656
MbiI CCGCTC 1 cut(s) 626
MboI GATC 2 cut(s) 390, 654
MboII GAAGA 5 cut(s) 83, 280, 384, 440, 443
MhlI GDGCHC 2 cut(s) 129, 213
MluCI AATT 7 cut(s) 106, 112, 219, 403, 412, 436, 645
MlyI GAGTC 1 cut(s) 146
MmeI TCCRAC 2 cut(s) 110, 222
MnlI CCTC 7 cut(s) 8, 93, 158, 184, 247, 504, 515
MroXI GAANNNNTTC 1 cut(s) 298
MseI TTAA 1 cut(s) 360
MslI CAYNNNNRTG 1 cut(s) 58
MspR9I CCNGG 4 cut(s) 7, 77, 420, 609
Mva1269I GAATGC 1 cut(s) 300
MvaI CCWGG 4 cut(s) 7, 77, 420, 609
MvnI CGCG 1 cut(s) 564
MwoI GCNNNNNNNGC 1 cut(s) 570
NdeI CATATG 1 cut(s) 337
NdeII GATC 2 cut(s) 390, 654
NlaIII CATG 3 cut(s) 63, 519, 577
NlaIV GGNNCC 3 cut(s) 81, 211, 333
NspI RCATGY 1 cut(s) 577
NspV TTCGAA 1 cut(s) 116
PctI GAATGC 1 cut(s) 300
PdmI GAANNNNTTC 1 cut(s) 298
PfeI GAWTC 1 cut(s) 490
PleI GAGTC 1 cut(s) 146
PpsI GAGTC 1 cut(s) 146
Psp124BI GAGCTC 1 cut(s) 129
Psp6I CCWGG 4 cut(s) 5, 75, 418, 607
PspEI GGTNACC 1 cut(s) 230
PspGI CCWGG 4 cut(s) 5, 75, 418, 607
PspN4I GGNNCC 3 cut(s) 81, 211, 333
PspOMI GGGCCC 1 cut(s) 209
PspPI GGNCC 3 cut(s) 209, 210, 331
RsaI GTAC 2 cut(s) 462, 479
RsaNI GTAC 2 cut(s) 461, 478
RseI CAYNNNNRTG 1 cut(s) 58
SacI GAGCTC 1 cut(s) 129
SaqAI TTAA 1 cut(s) 360
Sau3AI GATC 2 cut(s) 390, 654
Sau96I GGNCC 3 cut(s) 209, 210, 331
SchI GAGTC 1 cut(s) 146
ScrFI CCNGG 4 cut(s) 7, 77, 420, 609
SduI GDGCHC 2 cut(s) 129, 213
SetI ASST 9 cut(s) 11, 31, 78, 129, 237, 267, 421, 613, 642
SexAI ACCWGGT 2 cut(s) 75, 418
SfaNI GCATC 4 cut(s) 148, 188, 365, 526
SfuI TTCGAA 1 cut(s) 116
SinI GGWCC 1 cut(s) 331
SmiMI CAYNNNNRTG 1 cut(s) 58
SmlI CTYRAG 1 cut(s) 128
SmoI CTYRAG 1 cut(s) 128
Sse9I AATT 7 cut(s) 106, 112, 219, 403, 412, 436, 645
SsiI CCGC 2 cut(s) 600, 626
SspMI CTAG 1 cut(s) 615
SstI GAGCTC 1 cut(s) 129
StyD4I CCNGG 4 cut(s) 5, 75, 418, 607
TaqI TCGA 4 cut(s) 13, 116, 534, 642
TasI AATT 7 cut(s) 106, 112, 219, 403, 412, 436, 645
TfiI GAWTC 1 cut(s) 490
Tru1I TTAA 1 cut(s) 360
Tru9I TTAA 1 cut(s) 360
TscAI CASTG 2 cut(s) 220, 357
TspDTI ATGAA 3 cut(s) 216, 238, 566
TspGWI ACGGA 1 cut(s) 177
TspRI CASTG 2 cut(s) 220, 357
VpaK11BI GGWCC 1 cut(s) 331
XapI RAATTY 2 cut(s) 219, 412
XceI RCATGY 1 cut(s) 577
XmnI GAANNNNTTC 1 cut(s) 298
XspI CTAG 1 cut(s) 615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.