MD17G1150700.v1.1

complex I intermediate-associated protein 30

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0005050
Physical Location & Seq
Reverse (-)
13865650 .. 13869764
4115 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1150700.v1.1.491

Sequence Viewer

Length: 705 bp
ATGCTGGACGGAAACTTTGTGGAAAGAGAAATGTCCAGGTTTCGAGGGATATTGCAAGCTTCCTTAAATGCAACCAAGAAAGCACTTACATGGAACGTTGAAGACCTGGTGCCTCCAACTGAGAGATTTATTTTCAATTTCAATTCGAAGGACGAGCTCAAGAAGTGGCATCTATATTCTGACTCTGAATATGGAGGGTTGTCCTCGGCATCTTTAGAGATCCCAGATAATGAAAATGGACCCACTGGAATTTTCTCGGGTAACCTTTCGTTGGATTTCATTGAGGGTGCAAAACTGAAGATAAACAGGAGCGGTTTTTGTGGAATGCGGTCCAAAAAGTTTGACGGCTTCATTGATTTGGACCCATACGATACCATAGCACTGAAAGTTAAGGGAGATGGAAGGTGCTACATATCTACAATCTATACAGAAAATTGGGTAAATTCGCCTGGGCAAGAAGAAGATAATTCGTGGCAGGCTTTTGTCTTTGTACCAAAAGACAACTGGTACATTGCAAAGATTCCTCTTGCTCGGTATCTACCAACATGGAGAGGAAATGTTATAGATGCAGAAATGGAAATGAATCCTTCTCGCGTCGTTGGGATGTCTCTATCGGTCAATGCAGAAGGTGGTCTCCCAGGTGCTAGAACCGGAGCGGGTGATTTCAAGCTCGAAATTGATTGGATCAAGGCTTTACGAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
CIA30

Protein Analysis

235

Amino Acids

26.31

Weight (kDa)

5.12

Isoelectric Point (pI)

27.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CIA30 PF08547 45 - 226 1.6e-43 Complex I intermediate-associated protein 30 (CIA30)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 109
AccBSI CCGCTC 2 cut(s) 312, 656
AccII CGCG 1 cut(s) 594
AciI CCGC 3 cut(s) 312, 328, 656
AclI AACGTT 1 cut(s) 96
AclWI GGATC 2 cut(s) 214, 692
AcsI RAATTY 2 cut(s) 249, 442
AcuI CTGAAG 1 cut(s) 317
AfaI GTAC 2 cut(s) 492, 509
AfiI CCNNNNNNNGG 1 cut(s) 271
AgsI TTSAA 4 cut(s) 101, 136, 142, 667
AjnI CCWGG 4 cut(s) 35, 105, 448, 637
AluBI AGCT 3 cut(s) 59, 157, 670
AluI AGCT 3 cut(s) 59, 157, 670
Alw21I GWGCWC 1 cut(s) 159
Alw26I GTCTC 2 cut(s) 612, 638
AlwI GGATC 2 cut(s) 214, 692
Ama87I CYCGRG 1 cut(s) 256
ApoI RAATTY 2 cut(s) 249, 442
ArsI GACNNNNNNTTYG 1 cut(s) 31
AspS9I GGNCC 3 cut(s) 239, 330, 361
AsuHPI GGTGA 1 cut(s) 671
AsuII TTCGAA 1 cut(s) 146
AvaI CYCGRG 1 cut(s) 256
AvaII GGWCC 3 cut(s) 239, 330, 361
BanI GGYRCC 1 cut(s) 109
BanII GRGCYC 1 cut(s) 159
BbsI GAAGAC 1 cut(s) 108
Bbv12I GWGCWC 1 cut(s) 159
BccI CCATC 1 cut(s) 392
BceAI ACGGC 1 cut(s) 361
BciT130I CCWGG 4 cut(s) 37, 107, 450, 639
BcoDI GTCTC 2 cut(s) 612, 638
BfaI CTAG 1 cut(s) 645
Bme1390I CCNGG 4 cut(s) 37, 107, 450, 639
Bme18I GGWCC 3 cut(s) 239, 330, 361
BmeT110I CYCGRG 1 cut(s) 256
BmgT120I GGNCC 3 cut(s) 239, 330, 361
BmiI GGNNCC 3 cut(s) 111, 241, 363
BmrFI CCNGG 4 cut(s) 37, 107, 450, 639
BmsI GCATC 3 cut(s) 178, 218, 556
BpiI GAAGAC 1 cut(s) 108
Bpu14I TTCGAA 1 cut(s) 146
BpuEI CTTGAG 1 cut(s) 143
BsaI GGTCTC 1 cut(s) 638
BsaJI CCNNGG 3 cut(s) 204, 449, 637
BsaWI WCCGGW 1 cut(s) 650
Bsc4I CCNNNNNNNGG 1 cut(s) 271
Bse1I ACTGG 2 cut(s) 250, 509
Bse3DI GCAATG 1 cut(s) 510
BseBI CCWGG 4 cut(s) 37, 107, 450, 639
BseDI CCNNGG 3 cut(s) 204, 449, 637
BseGI GGATG 1 cut(s) 609
BseLI CCNNNNNNNGG 1 cut(s) 271
BseMI GCAATG 1 cut(s) 510
BseMII CTCAG 1 cut(s) 111
BseNI ACTGG 2 cut(s) 250, 509
Bsh1236I CGCG 1 cut(s) 594
BshNI GGYRCC 1 cut(s) 109
BsiHKAI GWGCWC 1 cut(s) 159
BsiHKCI CYCGRG 1 cut(s) 256
BsiSI CCGG 1 cut(s) 651
BslI CCNNNNNNNGG 1 cut(s) 271
BsmAI GTCTC 2 cut(s) 612, 638
BsmI GAATGC 1 cut(s) 330
Bso31I GGTCTC 1 cut(s) 638
BsoBI CYCGRG 1 cut(s) 256
Bsp119I TTCGAA 1 cut(s) 146
Bsp1286I GDGCHC 1 cut(s) 159
Bsp143I GATC 2 cut(s) 219, 684
BspACI CCGC 3 cut(s) 312, 328, 656
BspCNI CTCAG 1 cut(s) 112
BspFNI CGCG 1 cut(s) 594
BspLI GGNNCC 3 cut(s) 111, 241, 363
BspPI GGATC 2 cut(s) 214, 692
BspT104I TTCGAA 1 cut(s) 146
BspT107I GGYRCC 1 cut(s) 109
BspTNI GGTCTC 1 cut(s) 638
BsrBI CCGCTC 2 cut(s) 312, 656
BsrDI GCAATG 1 cut(s) 510
BsrI ACTGG 2 cut(s) 250, 509
BssECI CCNNGG 3 cut(s) 204, 449, 637
BssMI GATC 2 cut(s) 219, 684
Bst2UI CCWGG 4 cut(s) 37, 107, 450, 639
BstBI TTCGAA 1 cut(s) 146
BstC8I GCNNGC 2 cut(s) 57, 477
BstDEI CTNAG 1 cut(s) 120
BstEII GGTNACC 1 cut(s) 260
BstF5I GGATG 1 cut(s) 609
BstFNI CGCG 1 cut(s) 594
BstKTI GATC 2 cut(s) 222, 687
BstMAI GTCTC 2 cut(s) 612, 638
BstMBI GATC 2 cut(s) 219, 684
BstNI CCWGG 4 cut(s) 37, 107, 450, 639
BstPI GGTNACC 1 cut(s) 260
BstSCI CCNGG 4 cut(s) 35, 105, 448, 637
BstUI CGCG 1 cut(s) 594
BstV2I GAAGAC 1 cut(s) 108
BstX2I RGATCY 1 cut(s) 219
BstYI RGATCY 1 cut(s) 219
BtsCI GGATG 1 cut(s) 609
BtsIMutI CAGTG 2 cut(s) 243, 380
Cac8I GCNNGC 2 cut(s) 57, 477
Cfr13I GGNCC 3 cut(s) 239, 330, 361
CseI GACGC 1 cut(s) 583
CsiI ACCWGGT 1 cut(s) 105
Csp6I GTAC 2 cut(s) 491, 508
CviAII CATG 2 cut(s) 90, 546
CviJI RGCY 6 cut(s) 59, 157, 348, 479, 670, 692
CviKI_1 RGCY 6 cut(s) 59, 157, 348, 479, 670, 692
CviQI GTAC 2 cut(s) 491, 508
DdeI CTNAG 1 cut(s) 120
DpnI GATC 2 cut(s) 221, 686
DpnII GATC 2 cut(s) 219, 684
Ecl136II GAGCTC 1 cut(s) 157
Eco24I GRGCYC 1 cut(s) 159
Eco31I GGTCTC 1 cut(s) 638
Eco47I GGWCC 3 cut(s) 239, 330, 361
Eco53kI GAGCTC 1 cut(s) 157
Eco57I CTGAAG 1 cut(s) 317
Eco88I CYCGRG 1 cut(s) 256
Eco91I GGTNACC 1 cut(s) 260
EcoICRI GAGCTC 1 cut(s) 157
EcoO65I GGTNACC 1 cut(s) 260
EcoRII CCWGG 4 cut(s) 35, 105, 448, 637
EcoT38I GRGCYC 1 cut(s) 159
FaeI CATG 2 cut(s) 93, 549
FatI CATG 2 cut(s) 89, 545
FauI CCCGC 1 cut(s) 649
FokI GGATG 1 cut(s) 616
FriOI GRGCYC 1 cut(s) 159
FspBI CTAG 1 cut(s) 645
HapII CCGG 1 cut(s) 651
HgaI GACGC 1 cut(s) 583
Hin1II CATG 2 cut(s) 93, 549
HindIII AAGCTT 1 cut(s) 57
HinfI GANTC 3 cut(s) 182, 520, 583
HpaII CCGG 1 cut(s) 651
HphI GGTGA 1 cut(s) 671
Hpy188I TCNGA 2 cut(s) 181, 187
Hpy188III TCNNGA 1 cut(s) 160
Hpy99I CGWCG 1 cut(s) 599
HpyAV CCTTC 4 cut(s) 142, 396, 597, 620
HpyCH4IV ACGT 1 cut(s) 96
HpyCH4V TGCA 6 cut(s) 55, 71, 290, 515, 569, 623
HpyF3I CTNAG 1 cut(s) 120
HpySE526I ACGT 1 cut(s) 96
Hsp92II CATG 2 cut(s) 93, 549
Kzo9I GATC 2 cut(s) 219, 684
LmnI GCTCC 2 cut(s) 309, 653
LweI GCATC 3 cut(s) 178, 218, 556
MabI ACCWGGT 1 cut(s) 105
MaeI CTAG 1 cut(s) 645
MaeII ACGT 1 cut(s) 96
MaeIII GTNAC 1 cut(s) 260
MalI GATC 2 cut(s) 221, 686
MbiI CCGCTC 2 cut(s) 312, 656
MboI GATC 2 cut(s) 219, 684
MboII GAAGA 4 cut(s) 113, 310, 470, 473
MflI RGATCY 1 cut(s) 219
MhlI GDGCHC 1 cut(s) 159
MluCI AATT 7 cut(s) 136, 142, 249, 433, 442, 466, 675
MlyI GAGTC 1 cut(s) 176
MmeI TCCRAC 2 cut(s) 140, 252
MnlI CCTC 7 cut(s) 38, 123, 188, 214, 277, 534, 545
MseI TTAA 2 cut(s) 65, 390
MslI CAYNNNNRTG 1 cut(s) 88
MspI CCGG 1 cut(s) 651
MspR9I CCNGG 4 cut(s) 37, 107, 450, 639
Mva1269I GAATGC 1 cut(s) 330
MvaI CCWGG 4 cut(s) 37, 107, 450, 639
MvnI CGCG 1 cut(s) 594
NdeII GATC 2 cut(s) 219, 684
NlaIII CATG 2 cut(s) 93, 549
NlaIV GGNNCC 3 cut(s) 111, 241, 363
NmeAIII GCCGAG 1 cut(s) 185
NspV TTCGAA 1 cut(s) 146
PctI GAATGC 1 cut(s) 330
PfeI GAWTC 2 cut(s) 520, 583
PleI GAGTC 1 cut(s) 176
PpsI GAGTC 1 cut(s) 176
Psp124BI GAGCTC 1 cut(s) 159
Psp1406I AACGTT 1 cut(s) 96
Psp6I CCWGG 4 cut(s) 35, 105, 448, 637
PspEI GGTNACC 1 cut(s) 260
PspGI CCWGG 4 cut(s) 35, 105, 448, 637
PspN4I GGNNCC 3 cut(s) 111, 241, 363
PspPI GGNCC 3 cut(s) 239, 330, 361
PsuI RGATCY 1 cut(s) 219
RsaI GTAC 2 cut(s) 492, 509
RsaNI GTAC 2 cut(s) 491, 508
RseI CAYNNNNRTG 1 cut(s) 88
SacI GAGCTC 1 cut(s) 159
SaqAI TTAA 2 cut(s) 65, 390
Sau3AI GATC 2 cut(s) 219, 684
Sau96I GGNCC 3 cut(s) 239, 330, 361
SchI GAGTC 1 cut(s) 176
ScrFI CCNGG 4 cut(s) 37, 107, 450, 639
SduI GDGCHC 1 cut(s) 159
SexAI ACCWGGT 1 cut(s) 105
SfaNI GCATC 3 cut(s) 178, 218, 556
SfuI TTCGAA 1 cut(s) 146
SinI GGWCC 3 cut(s) 239, 330, 361
SmiMI CAYNNNNRTG 1 cut(s) 88
SmlI CTYRAG 1 cut(s) 158
SmoI CTYRAG 1 cut(s) 158
Sse9I AATT 7 cut(s) 136, 142, 249, 433, 442, 466, 675
SsiI CCGC 3 cut(s) 312, 328, 656
SspMI CTAG 1 cut(s) 645
SstI GAGCTC 1 cut(s) 159
StyD4I CCNGG 4 cut(s) 35, 105, 448, 637
TaiI ACGT 1 cut(s) 99
TaqI TCGA 3 cut(s) 43, 146, 672
TaqII GACCGA 1 cut(s) 604
TasI AATT 7 cut(s) 136, 142, 249, 433, 442, 466, 675
TfiI GAWTC 2 cut(s) 520, 583
Tru1I TTAA 2 cut(s) 65, 390
Tru9I TTAA 2 cut(s) 65, 390
TscAI CASTG 2 cut(s) 250, 387
TspDTI ATGAA 4 cut(s) 246, 268, 340, 596
TspGWI ACGGA 1 cut(s) 24
TspRI CASTG 2 cut(s) 250, 387
VpaK11BI GGWCC 3 cut(s) 239, 330, 361
XapI RAATTY 2 cut(s) 249, 442
XcmI CCANNNNNNNNNTGG 1 cut(s) 501
XspI CTAG 1 cut(s) 645
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.