FvH4_6g36970

Protein of unknown function (DUF3754)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0005827
Physical Location & Seq
Forward (+)
29040114 .. 29047428
7315 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g36970.t3

Sequence Viewer

Length: 2160 bp
ATGTCAGCTTCTTCTTGGCACACTCTCACCAGCCTCACCGCCCTCTCTTCCTTGACCTCAAAACGCCGTCGTATTCTTCCCTCACCACCTCACTCCCTCACAGCTCCCCGCTGCTCTTCTTCCCTCACCGGCTTCCGAGAAATCTCCGCCGAGCCTAAGCAGCCCCAGCCCGACGCTACAAGCTTCACCGAAGCGCTCAAGAAGCAAAACGACGTCGCCTCGCGGAACAATGAAGACGACGAAGACGAAGAGGATCAGGCAATTTCCAAAGTTCCGGTTCCCAGGCAGAAGTACATCCCGGTCTCCAAGGCCGAGCTGCTAGACGGCGTCGTTTCGAAGCTCTTTCCGGATGATGACGGTGATTTGGAGCAGTTTCTTCTTCTCTCTAAGTGCTTGGATTCGATTCTGCATGCGGAGCATAAGAGCATATTGGAGGAAATGCGAGCCGATTATTTGGATTCGAATTCTGTGGAGTACAAGCAAGCAATGGAGGAGTTCAGTGAAGAAGCAATGGCTAAGGGGATCAATGGAGTTGGGAGTGTAGATGGAAATGAGAGTGATGACAATGAAGCTGAAAACTCGATGCGGTTCGATTATGTTGAGGGATTGAGGAAACTGTTGGGGTCTTCAGTTAAGGACGTCAAAAGGGTTGCTGTTGCCACTCGTTTCCAGCGCTCTTTCATGCAGCTTTTGTATGACGCTCAGTTTGAAGAACTATCAGCGAGGGATTTAATGCTGACATCTGCATTGAACTCAGATTACCTCCTTACTTTGCCAATATATGTCGACTGGAAGAAGGCTTCCGAGTCCAATGCAATAATATACAGGCGGGGATATGCAACTGAAAGGCAGAAGGGTCTACTACTTGTTGAAAAACTGGAGTACATACAGTCCAAACTTCTACGAGGAATCTTCTTCATTGTATCAAAACCATTGGGGGAAATTGGGAGCTGGATAACAGAGTCATTGAAAGTTGCTTCTCAGACACCAGAGGTACAAGATTGGACTGAAAAAATGAAAGTTTGGCTAAAGGAACTCTCTGTTTTTCAGCAAGCATTTCGTACTAACGAACAAACATCCGATGATATGCTGGGGGTAGATCAACTATCAGATAGGGAACTTCCGATATGGCTGGCAGCACAGAGAGCTGTATCTCGTTATGAAGGAATATTATCACCAGTTGGACCCCGTGGAAGGCTCTTAAGGAAGTTGCTTTCATGGATTGGACTTATTTCCCTTACCCCAGAAACACCATTTGAGCTTGATGGCGATGCTAATGCTTCTGATCCTTATGCAAGGCCCATCTTCTTATCAAGGAGATCACTCAGTGACATATGGAGACCTGCTTCATGGAAATACTGTGATAAGAATATTTGGAAAATGTTGAAGACTTCTGTATCCATTCTTTTCTCAAAGTCAATCCTCCAGGAAGCAGCATATCAAGAATTGATTTTGCTTTATACCAAAGAACGAGTTGACAGGAACACTGAAGATAGTGCTTCGGTTCCATCGTTGCAGTTAAAGATTTATGAGAAAATCCCTATTCCGGATCTACCAGTCATCTTTCCCCACAAAAAGCTCTCTTTCCGTATCATAGACACGGTACGCTTGGACATTGCTTCAACATTGGCTCTTTCAGCGTACTTCATAAACTACAAATTTGTTAATGTGTTATCTTCGCCGTCAGCAATATATCTTGATGTGGTCGCTATTAGTGCTCTTGTAATATATATCTCCCGTGTGGTTCTGGGATACAAACAGACCTGGGATAGGTATCAACTTTTGGTCAACACGACGCTTTATGAGAAAACCTTAGCTAGTGGATTCGGCTCAGTTCATTTTTTGCTGGATGCTTCTGAACAGCAACAATACAAGGAAGCGATTTTGTCATATGCAATCCTTCTCAAAGCTAAGGAGGGAAAGGTTTGTCTCCTGCTTACAAATCGTGAGAGTGTTGGGGACAAATGCGAAAGATTTATGTATGATGTGTTTAAAGTAAAGGTTGAGATGCCAGTTGACAAGGCAATTGCTACATTATTAAGACTGGGGCTTGCTGCAGAAACAAGTATCGATGGACGGAGTACTCTAGAGGCTATTTCCTGTTCCAGTGCATACGATTCCCTTAAACAACGTTGGAATAGTTTACTTGATCAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3754

Protein Analysis

720

Amino Acids

81.29

Weight (kDa)

5.46

Isoelectric Point (pI)

45.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3754 PF12576 451 - 581 8e-32 Protein of unknown function (DUF3754)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 216, 642
Acc36I ACCTGC 1 cut(s) 1351
AccI GTMKAC 2 cut(s) 786, 859
AccII CGCG 1 cut(s) 223
AccIII TCCGGA 2 cut(s) 346, 1546
AciI CCGC 7 cut(s) 39, 109, 147, 223, 413, 586, 829
AclI AACGTT 1 cut(s) 2131
AclWI GGATC 4 cut(s) 261, 530, 1280, 1557
AcsI RAATTY 2 cut(s) 463, 1658
AcuI CTGAAG 2 cut(s) 612, 1509
AcyI GRCGYC 3 cut(s) 213, 327, 639
AdeI CACNNNGTG 1 cut(s) 1328
AfaI GTAC 8 cut(s) 293, 476, 884, 996, 1063, 1605, 1643, 2083
AfeI AGCGCT 2 cut(s) 195, 674
AfiI CCNNNNNNNGG 3 cut(s) 1194, 1546, 1770
AflII CTTAAG 1 cut(s) 1201
AgsI TTSAA 6 cut(s) 710, 751, 872, 970, 1387, 1623
AjnI CCWGG 3 cut(s) 281, 1425, 1763
Alw21I GWGCWC 1 cut(s) 1720
Alw26I GTCTC 3 cut(s) 307, 1333, 1934
AlwI GGATC 4 cut(s) 261, 530, 1280, 1557
Aor13HI TCCGGA 2 cut(s) 346, 1546
Aor51HI AGCGCT 2 cut(s) 195, 674
AoxI GGCC 2 cut(s) 309, 1298
ApeKI GCWGC 7 cut(s) 111, 160, 316, 685, 1136, 1433, 2054
ApoI RAATTY 2 cut(s) 463, 1658
ArsI GACNNNNNNTTYG 2 cut(s) 689, 721
AspLEI GCGC 2 cut(s) 196, 675
AspS9I GGNCC 2 cut(s) 1184, 1299
AsuC2I CCSGG 1 cut(s) 299
AsuHPI GGTGA 7 cut(s) 19, 28, 75, 118, 178, 371, 1167
AsuII TTCGAA 2 cut(s) 335, 461
AvaII GGWCC 1 cut(s) 1184
BaeI ACNNNNGTAYC 2 cut(s) 1380, 1413
BbsI GAAGAC 4 cut(s) 240, 249, 618, 1394
Bbv12I GWGCWC 1 cut(s) 1720
BbvI GCAGC 7 cut(s) 98, 172, 303, 697, 1148, 1445, 2041
BccI CCATC 5 cut(s) 539, 1259, 1310, 1516, 2066
BceAI ACGGC 3 cut(s) 51, 340, 1666
BcgI CGANNNNNNTGC 6 cut(s) 794, 828, 1040, 1074, 1259, 1293
BciT130I CCWGG 3 cut(s) 283, 1427, 1765
BciVI GTATCC 2 cut(s) 1408, 1745
BclI TGATCA 1 cut(s) 2149
BcnI CCSGG 1 cut(s) 299
BcoDI GTCTC 3 cut(s) 307, 1333, 1934
BfaI CTAG 3 cut(s) 320, 1818, 2087
BfmI CTRYAG 1 cut(s) 2055
BfoI RGCGCY 2 cut(s) 197, 676
BfrI CTTAAG 1 cut(s) 1201
BfuAI ACCTGC 1 cut(s) 1351
BfuI GTATCC 2 cut(s) 1408, 1745
BisI GCNGC 7 cut(s) 112, 161, 317, 686, 1137, 1434, 2055
BlsI GCNGC 7 cut(s) 113, 162, 318, 687, 1138, 1435, 2056
BmcAI AGTACT 1 cut(s) 2083
Bme1390I CCNGG 4 cut(s) 283, 299, 1427, 1765
Bme18I GGWCC 1 cut(s) 1184
BmgT120I GGNCC 2 cut(s) 1184, 1299
BmiI GGNNCC 3 cut(s) 279, 1186, 1506
BmrFI CCNGG 4 cut(s) 283, 299, 1427, 1765
BmrI ACTGGG 1 cut(s) 2054
BmsI GCATC 4 cut(s) 573, 1261, 1840, 1998
BmuI ACTGGG 1 cut(s) 2054
BpiI GAAGAC 4 cut(s) 240, 249, 618, 1394
BpmI CTGGAG 2 cut(s) 899, 1409
Bpu10I CCTNAGC 4 cut(s) 156, 516, 1813, 1911
Bpu14I TTCGAA 2 cut(s) 335, 461
BpuEI CTTGAG 1 cut(s) 182
BpuMI CCSGG 1 cut(s) 299
Bsa29I ATCGAT 1 cut(s) 2070
BsaBI GATNNNNATC 1 cut(s) 1773
BsaHI GRCGYC 3 cut(s) 213, 327, 639
BsaI GGTCTC 2 cut(s) 307, 1333
BsaJI CCNNGG 4 cut(s) 281, 306, 1189, 1764
BsaWI WCCGGW 3 cut(s) 274, 346, 1546
Bsc4I CCNNNNNNNGG 3 cut(s) 1194, 1546, 1770
Bse118I RCCGGY 1 cut(s) 128
Bse1I ACTGG 7 cut(s) 794, 882, 1178, 1556, 2012, 2049, 2106
Bse3DI GCAATG 3 cut(s) 492, 516, 1614
Bse8I GATNNNNATC 1 cut(s) 1773
BseAI TCCGGA 2 cut(s) 346, 1546
BseBI CCWGG 3 cut(s) 283, 1427, 1765
BseCI ATCGAT 1 cut(s) 2070
BseDI CCNNGG 4 cut(s) 281, 306, 1189, 1764
BseGI GGATG 4 cut(s) 294, 355, 1076, 1855
BseJI GATNNNNATC 1 cut(s) 1773
BseLI CCNNNNNNNGG 3 cut(s) 1194, 1546, 1770
BseMI GCAATG 3 cut(s) 492, 516, 1614
BseMII CTCAG 5 cut(s) 716, 768, 995, 1339, 1845
BseNI ACTGG 7 cut(s) 794, 882, 1178, 1556, 2012, 2049, 2106
BseRI GAGGAG 1 cut(s) 506
BseXI GCAGC 7 cut(s) 98, 172, 303, 697, 1148, 1445, 2041
BseYI CCCAGC 2 cut(s) 165, 1090
Bsh1236I CGCG 1 cut(s) 223
BshFI GGCC 2 cut(s) 311, 1300
BshVI ATCGAT 1 cut(s) 2070
BsiHKAI GWGCWC 1 cut(s) 1720
BsiSI CCGG 5 cut(s) 129, 275, 299, 347, 1547
BslFI GGGAC 1 cut(s) 1973
BslI CCNNNNNNNGG 3 cut(s) 1194, 1546, 1770
BsmAI GTCTC 3 cut(s) 307, 1333, 1934
BsmFI GGGAC 1 cut(s) 1973
BsnI GGCC 2 cut(s) 311, 1300
Bso31I GGTCTC 2 cut(s) 307, 1333
Bsp119I TTCGAA 2 cut(s) 335, 461
Bsp1286I GDGCHC 1 cut(s) 1720
Bsp13I TCCGGA 2 cut(s) 346, 1546
Bsp143I GATC 7 cut(s) 253, 522, 1099, 1285, 1319, 1549, 2149
BspACI CCGC 7 cut(s) 39, 109, 147, 223, 413, 586, 829
BspANI GGCC 2 cut(s) 311, 1300
BspCNI CTCAG 5 cut(s) 715, 767, 994, 1338, 1844
BspDI ATCGAT 1 cut(s) 2070
BspEI TCCGGA 2 cut(s) 346, 1546
BspFNI CGCG 1 cut(s) 223
BspLI GGNNCC 3 cut(s) 279, 1186, 1506
BspMAI CTGCAG 1 cut(s) 2059
BspMI ACCTGC 1 cut(s) 1351
BspPI GGATC 4 cut(s) 261, 530, 1280, 1557
BspQI GCTCTTC 1 cut(s) 121
BspT104I TTCGAA 2 cut(s) 335, 461
BspTI CTTAAG 1 cut(s) 1201
BspTNI GGTCTC 2 cut(s) 307, 1333
BsrDI GCAATG 3 cut(s) 492, 516, 1614
BsrFI RCCGGY 1 cut(s) 128
BsrI ACTGG 7 cut(s) 794, 882, 1178, 1556, 2012, 2049, 2106
BssAI RCCGGY 1 cut(s) 128
BssECI CCNNGG 4 cut(s) 281, 306, 1189, 1764
BssMI GATC 7 cut(s) 253, 522, 1099, 1285, 1319, 1549, 2149
BssNI GRCGYC 3 cut(s) 213, 327, 639
BssT1I CCWWGG 1 cut(s) 306
Bst2UI CCWGG 3 cut(s) 283, 1427, 1765
Bst4CI ACNGT 5 cut(s) 359, 618, 891, 1361, 1603
Bst6I CTCTTC 3 cut(s) 52, 121, 243
BstACI GRCGYC 3 cut(s) 213, 327, 639
BstAFI CTTAAG 1 cut(s) 1201
BstBI TTCGAA 2 cut(s) 335, 461
BstC8I GCNNGC 6 cut(s) 411, 444, 483, 1053, 1134, 2052
BstDSI CCRYGG 1 cut(s) 1189
BstENI CCTNNNNNAGG 1 cut(s) 1768
BstF5I GGATG 4 cut(s) 294, 355, 1076, 1855
BstFNI CGCG 1 cut(s) 223
BstH2I RGCGCY 2 cut(s) 197, 676
BstHHI GCGC 2 cut(s) 196, 675
BstKTI GATC 7 cut(s) 256, 525, 1102, 1288, 1322, 1552, 2152
BstMAI GTCTC 3 cut(s) 307, 1333, 1934
BstMBI GATC 7 cut(s) 253, 522, 1099, 1285, 1319, 1549, 2149
BstMWI GCNNNNNNNGC 7 cut(s) 160, 166, 202, 415, 1145, 1637, 1715
BstNI CCWGG 3 cut(s) 283, 1427, 1765
BstNSI RCATGY 1 cut(s) 413
BstSCI CCNGG 4 cut(s) 281, 297, 1425, 1763
BstSFI CTRYAG 1 cut(s) 2055
BstUI CGCG 1 cut(s) 223
BstV1I GCAGC 7 cut(s) 98, 172, 303, 697, 1148, 1445, 2041
BstV2I GAAGAC 4 cut(s) 240, 249, 618, 1394
BstX2I RGATCY 1 cut(s) 1549
BstYI RGATCY 1 cut(s) 1549
Bsu15I ATCGAT 1 cut(s) 2070
BsuI GTATCC 2 cut(s) 1408, 1745
BsuRI GGCC 2 cut(s) 311, 1300
BsuTUI ATCGAT 1 cut(s) 2070
BtgI CCRYGG 1 cut(s) 1189
BtgZI GCGATG 1 cut(s) 1284
BtsCI GGATG 4 cut(s) 294, 355, 1076, 1855
BtsIMutI CAGTG 4 cut(s) 505, 1333, 1485, 2113
BveI ACCTGC 1 cut(s) 1351
Cac8I GCNNGC 6 cut(s) 411, 444, 483, 1053, 1134, 2052
CfoI GCGC 2 cut(s) 196, 675
Cfr10I RCCGGY 1 cut(s) 128
Cfr13I GGNCC 2 cut(s) 1184, 1299
ClaI ATCGAT 1 cut(s) 2070
CseI GACGC 4 cut(s) 182, 316, 707, 1804
Csp6I GTAC 8 cut(s) 292, 475, 883, 995, 1062, 1604, 1642, 2082
CviAII CATG 4 cut(s) 410, 682, 1218, 1350
CviQI GTAC 8 cut(s) 292, 475, 883, 995, 1062, 1604, 1642, 2082
DpnI GATC 7 cut(s) 255, 524, 1101, 1287, 1321, 1551, 2151
DpnII GATC 7 cut(s) 253, 522, 1099, 1285, 1319, 1549, 2149
DraI TTTAAA 1 cut(s) 1993
DraIII CACNNNGTG 1 cut(s) 1328
Eam1104I CTCTTC 3 cut(s) 52, 121, 243
EarI CTCTTC 3 cut(s) 52, 121, 243
EciI GGCGGA 1 cut(s) 136
Eco130I CCWWGG 1 cut(s) 306
Eco31I GGTCTC 2 cut(s) 307, 1333
Eco47I GGWCC 1 cut(s) 1184
Eco47III AGCGCT 2 cut(s) 195, 674
Eco57I CTGAAG 2 cut(s) 612, 1509
EcoNI CCTNNNNNAGG 1 cut(s) 1768
EcoRI GAATTC 1 cut(s) 463
EcoRII CCWGG 3 cut(s) 281, 1425, 1763
EcoT14I CCWWGG 1 cut(s) 306
ErhI CCWWGG 1 cut(s) 306
FaeI CATG 4 cut(s) 413, 685, 1221, 1353
FaqI GGGAC 1 cut(s) 1973
FatI CATG 4 cut(s) 409, 681, 1217, 1349
FauI CCCGC 2 cut(s) 116, 822
FauNDI CATATG 2 cut(s) 1334, 1891
FbaI TGATCA 1 cut(s) 2149
FblI GTMKAC 2 cut(s) 786, 859
Fnu4HI GCNGC 7 cut(s) 112, 161, 317, 686, 1137, 1434, 2055
FokI GGATG 4 cut(s) 281, 362, 1063, 1862
Fsp4HI GCNGC 7 cut(s) 112, 161, 317, 686, 1137, 1434, 2055
FspBI CTAG 3 cut(s) 320, 1818, 2087
GlaI GCGC 2 cut(s) 195, 674
GluI GCNGC 7 cut(s) 112, 161, 317, 686, 1137, 1434, 2055
GsaI CCCAGC 2 cut(s) 169, 1094
GsuI CTGGAG 2 cut(s) 899, 1409
HaeII RGCGCY 2 cut(s) 197, 676
HaeIII GGCC 2 cut(s) 311, 1300
HapII CCGG 5 cut(s) 129, 275, 299, 347, 1547
HgaI GACGC 4 cut(s) 182, 316, 707, 1804
HhaI GCGC 2 cut(s) 196, 675
Hin1I GRCGYC 3 cut(s) 213, 327, 639
Hin1II CATG 4 cut(s) 413, 685, 1221, 1353
Hin6I GCGC 2 cut(s) 194, 673
HinP1I GCGC 2 cut(s) 194, 673
HincII GTYRAC 4 cut(s) 787, 1477, 1789, 2017
HindII GTYRAC 4 cut(s) 787, 1477, 1789, 2017
HindIII AAGCTT 1 cut(s) 181
HinfI GANTC 8 cut(s) 398, 403, 458, 806, 909, 962, 1824, 2117
HpaII CCGG 5 cut(s) 129, 275, 299, 347, 1547
HphI GGTGA 7 cut(s) 19, 28, 75, 118, 178, 371, 1167
Hpy166II GTNNAC 6 cut(s) 787, 860, 1477, 1789, 2017, 2144
Hpy188I TCNGA 9 cut(s) 137, 757, 805, 984, 1081, 1111, 1125, 1285, 1858
Hpy188III TCNNGA 8 cut(s) 199, 347, 1442, 1547, 1697, 1946, 2087, 2153
Hpy8I GTNNAC 6 cut(s) 787, 860, 1477, 1789, 2017, 2144
Hpy99I CGWCG 7 cut(s) 72, 176, 215, 218, 242, 332, 1798
HpyAV CCTTC 5 cut(s) 790, 847, 1157, 1188, 1910
HpyCH4III ACNGT 5 cut(s) 359, 618, 891, 1361, 1603
HpyCH4IV ACGT 3 cut(s) 213, 639, 2131
HpyF10VI GCNNNNNNNGC 7 cut(s) 160, 166, 202, 415, 1145, 1637, 1715
HpySE526I ACGT 3 cut(s) 213, 639, 2131
Hsp92I GRCGYC 3 cut(s) 213, 327, 639
Hsp92II CATG 4 cut(s) 413, 685, 1221, 1353
HspAI GCGC 2 cut(s) 194, 673
Kpn2I TCCGGA 2 cut(s) 346, 1546
Ksp22I TGATCA 1 cut(s) 2149
Kzo9I GATC 7 cut(s) 253, 522, 1099, 1285, 1319, 1549, 2149
LguI GCTCTTC 1 cut(s) 121
LmnI GCTCC 4 cut(s) 109, 367, 415, 948
Lsp1109I GCAGC 7 cut(s) 98, 172, 303, 697, 1148, 1445, 2041
LweI GCATC 4 cut(s) 573, 1261, 1840, 1998
MaeI CTAG 3 cut(s) 320, 1818, 2087
MaeII ACGT 3 cut(s) 213, 639, 2131
MaeIII GTNAC 1 cut(s) 1328
MalI GATC 7 cut(s) 255, 524, 1101, 1287, 1321, 1551, 2151
MboI GATC 7 cut(s) 253, 522, 1099, 1285, 1319, 1549, 2149
MfeI CAATTG 1 cut(s) 2025
MflI RGATCY 1 cut(s) 1549
MhlI GDGCHC 1 cut(s) 1720
MluCI AATT 6 cut(s) 261, 463, 942, 1445, 1658, 2025
MlyI GAGTC 2 cut(s) 815, 971
MmeI TCCRAC 2 cut(s) 1162, 2114
MroI TCCGGA 2 cut(s) 346, 1546
MseI TTAA 8 cut(s) 633, 731, 1202, 1520, 1665, 1992, 2039, 2124
MspA1I CMGCKG 1 cut(s) 111
MspCI CTTAAG 1 cut(s) 1201
MspI CCGG 5 cut(s) 129, 275, 299, 347, 1547
MspR9I CCNGG 4 cut(s) 283, 299, 1427, 1765
MunI CAATTG 1 cut(s) 2025
MvaI CCWGG 3 cut(s) 283, 1427, 1765
MvnI CGCG 1 cut(s) 223
MwoI GCNNNNNNNGC 7 cut(s) 160, 166, 202, 415, 1145, 1637, 1715
NciI CCSGG 1 cut(s) 299
NdeI CATATG 2 cut(s) 1334, 1891
NdeII GATC 7 cut(s) 253, 522, 1099, 1285, 1319, 1549, 2149
NlaIII CATG 4 cut(s) 413, 685, 1221, 1353
NlaIV GGNNCC 3 cut(s) 279, 1186, 1506
NmeAIII GCCGAG 2 cut(s) 175, 337
NmuCI GTSAC 1 cut(s) 1328
NspI RCATGY 1 cut(s) 413
NspV TTCGAA 2 cut(s) 335, 461
PaeI GCATGC 1 cut(s) 413
PciSI GCTCTTC 1 cut(s) 121
PcsI WCGNNNNNNNCGW 2 cut(s) 243, 1508
PfeI GAWTC 6 cut(s) 398, 403, 458, 909, 1824, 2117
PflFI GACNNNGTC 1 cut(s) 326
PfoI TCCNGGA 1 cut(s) 1425
PkrI GCNGC 7 cut(s) 113, 162, 318, 687, 1138, 1435, 2056
PleI GAGTC 2 cut(s) 814, 970
PpsI GAGTC 2 cut(s) 814, 970
Psp1406I AACGTT 1 cut(s) 2131
Psp6I CCWGG 3 cut(s) 281, 1425, 1763
PspFI CCCAGC 2 cut(s) 165, 1090
PspGI CCWGG 3 cut(s) 281, 1425, 1763
PspN4I GGNNCC 3 cut(s) 279, 1186, 1506
PspPI GGNCC 2 cut(s) 1184, 1299
PsrI GAACNNNNNNTAC 2 cut(s) 743, 775
PstI CTGCAG 1 cut(s) 2059
PsuI RGATCY 1 cut(s) 1549
PsyI GACNNNGTC 1 cut(s) 326
RsaI GTAC 8 cut(s) 293, 476, 884, 996, 1063, 1605, 1643, 2083
RsaNI GTAC 8 cut(s) 292, 475, 883, 995, 1062, 1604, 1642, 2082
SalI GTCGAC 1 cut(s) 785
SapI GCTCTTC 1 cut(s) 121
SaqAI TTAA 8 cut(s) 633, 731, 1202, 1520, 1665, 1992, 2039, 2124
SatI GCNGC 7 cut(s) 112, 161, 317, 686, 1137, 1434, 2055
Sau3AI GATC 7 cut(s) 253, 522, 1099, 1285, 1319, 1549, 2149
Sau96I GGNCC 2 cut(s) 1184, 1299
ScaI AGTACT 1 cut(s) 2083
SchI GAGTC 2 cut(s) 815, 971
ScrFI CCNGG 4 cut(s) 283, 299, 1427, 1765
SduI GDGCHC 1 cut(s) 1720
SfaNI GCATC 4 cut(s) 573, 1261, 1840, 1998
SfcI CTRYAG 1 cut(s) 2055
SfuI TTCGAA 2 cut(s) 335, 461
SinI GGWCC 1 cut(s) 1184
SmlI CTYRAG 2 cut(s) 197, 1201
SmoI CTYRAG 2 cut(s) 197, 1201
SphI GCATGC 1 cut(s) 413
Sse9I AATT 6 cut(s) 261, 463, 942, 1445, 1658, 2025
SsiI CCGC 7 cut(s) 39, 109, 147, 223, 413, 586, 829
SspI AATATT 2 cut(s) 1170, 1372
SspMI CTAG 3 cut(s) 320, 1818, 2087
StyD4I CCNGG 4 cut(s) 281, 297, 1425, 1763
StyI CCWWGG 1 cut(s) 306
TaaI ACNGT 5 cut(s) 359, 618, 891, 1361, 1603
TaiI ACGT 3 cut(s) 216, 642, 2134
TaqI TCGA 7 cut(s) 335, 401, 461, 581, 591, 786, 2070
TasI AATT 6 cut(s) 261, 463, 942, 1445, 1658, 2025
TatI WGTACW 4 cut(s) 291, 474, 882, 2081
TfiI GAWTC 6 cut(s) 398, 403, 458, 909, 1824, 2117
Tru1I TTAA 8 cut(s) 633, 731, 1202, 1520, 1665, 1992, 2039, 2124
Tru9I TTAA 8 cut(s) 633, 731, 1202, 1520, 1665, 1992, 2039, 2124
TscAI CASTG 4 cut(s) 505, 1333, 1492, 2113
TseFI GTSAC 1 cut(s) 1328
TseI GCWGC 7 cut(s) 111, 160, 316, 685, 1136, 1433, 2054
Tsp45I GTSAC 1 cut(s) 1328
TspGWI ACGGA 2 cut(s) 1577, 2092
TspRI CASTG 4 cut(s) 505, 1333, 1492, 2113
Tth111I GACNNNGTC 1 cut(s) 326
Vha464I CTTAAG 1 cut(s) 1201
VpaK11BI GGWCC 1 cut(s) 1184
XagI CCTNNNNNAGG 1 cut(s) 1768
XapI RAATTY 2 cut(s) 463, 1658
XbaI TCTAGA 1 cut(s) 2086
XceI RCATGY 1 cut(s) 413
XmiI GTMKAC 2 cut(s) 786, 859
XspI CTAG 3 cut(s) 320, 1818, 2087
ZraI GACGTC 2 cut(s) 214, 640
ZrmI AGTACT 1 cut(s) 2083
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.