RH2CG457800

Protein of unknown function (DUF3754)

Design Primers CRISPR Design

Basic Information

Type: Sequence Only
Biological Identity
rosa_samantha
Unknown
OG0005827
Physical Location & Seq
Reverse (-)
0 .. 0
1 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG457800.1

Sequence Viewer

Length: 2142 bp
ATGTCAGCTTCTTACCACACTCTCACCAGCCTCACCGCTCTCTCCTTGACCTCAAAACGCCGTCGTTCTACTCCTTCAACACCTCACTCGCTCACACTCGCTCGCTCTTCCCTCACCGGCTTCCGAGAAATCTCCACCGAGCCTAGGCAACCCCAAACCGACGCTGTAAACCTCACAGAAGCGCCGCTTCTTAAGAAGCAGAACGACGTCGTCCCGCAGAAGAAGAAGCGAGACGATGAAGACGAAGATGGAGATCAGGCGATTTCGAAGATTCCGGTTCCCAGGCAGAAGTACATCCCGGTCTCCAAGGCCGAGCTGCTAGACGGCGTCGTTTCGAATCTCTTTCGAGACCAGAACGGCGATGATTTGGAGCAGTTTCTTCTTCTTTCATCGTGCTTGGATTCAATTCTTCATGCTGAGCACAAAAAGATTCTGGAAGAAATGCGAGCTGATTATTCGGAATCCAACTCCATGGAGTACAAGCAAGCAATTGAAGAGTTAGCAATGGCTAATGGGCAGGGCTCGGATTCTGCTGAAGGAATCAATGGAGTTGGGAGTGAAGAAAATGAAGCTGAAATTCCGTTCGATTATGTTAAGGGATTGAGGAAACTCTTGGGTTCTTCAGCTAAGGACGTCAAAAGAGTTGCCGTAGCCACTCGTTTCCAGCGCTCTTTCATGCAGCTTCTTTATGACGCTCAGTTTGAAGAACTATCACCAAGGGATTTAATGTTGACATCTGCATTAAACTCAGATTATCTCCTTACTTTGCCAATATATGTTGACTGGAAGAAGGCTTCTCAGTCCAATGCAATAATATACAGGCGGGGATATGCAACTGAAAGGCAGAAGGGTCTACTACTTGTTGAAAAACTAGAGTACATACAGTCCAAACTTCTACGAGGAATCTTCTTCATTGTATCGAAACCATTGGGGGAAATCGGGAGCTGGATAACTGAGTCATTGAAAGTTGCTCTTCAGACACCAGAGGTACAAGAATGGACTGAAAAAATGAGACTTTGGCTAAAGGAATTCTCTGTTATTCAGCAAGCATTTCGTACTAACGAACAAAGTTCTGATGATCTGCTGGGAGTAGATCAACTATCGGATAGGGAACTTCCAATATGGCTGGCAGCACAGAGAGCTGTATCTCGTTATGAAGGAATTTTATCCCCAGTTGGACCCCGTGGAAGGCTCTTAAGGAAGTTGCTTTCATGGATTGGACTTATTTCCCTTACAGCAGAAACACCATTTGAGTTTGATGCTGATGCTAATGCTTCTGATCCTTATGCAAGGCCCATCTTCTTATCAAGGATATCACTCAGTGACATATGGAGACCTGCTACATGGAAATACTGTGAAAAGAATATATGGAAAATGTTGAAGACTTCTGTTTCCATTCTTTTCTCAAAGTCAATCCTCCAGGAGGCAGCATATCAAGAATTGATTTTGCTGTATACCAAAGAAGGAGATGACAGGAGTACTGAAGATAATGCTTCAGTTCCATCGTTGCAGTTAAAGATTTATGAGAAAATCCCTATTCCGGATCTACCAGTCATCTTTCCCCACAAAAAGCTCTCTTTCCGTATCATAGACACGGTGCGCTTGGACATTGCCTCAACATTGGCGCTTTCAGCATACTTCATAAACTACAAATTTGTTAATGTCTTATCTTCGCCGTCAGCAATATATCTTGATGTGGTCGCTATCACTGCTCTTGTAATATATATCTCCCGTGTGGTTCTGGGATACAAACAGACCTGGGATAGGTATCAACTTTTGGTCAACAAGACGCTTTATGAGAAAACCTTAGCTAGTGGATTTGGTTCAGTTCATTTTTTGCTGGATGCTTCTGAACAGCAACAATACAAGGAAGCCATTTTGGCGTATGCAATCCTTCTCAAAGCTAAGGAGGGAAAGCTTACAAGTCGCGGGAGTGTTGGAGACACATGCGAAAGATTTATGTATGATGTGTTTAAAGTAAAGGTTGAAATGCCAGTTGACAAGGCAATTGCTACATTAGTAAGACTGGGGCTTGCTGCAGAAACAAGTATTGATGGAAGGATTACTCTGCAAGCTATTCCATGTTCGAGTGCATATGATTCCCTTAAACAACGTTGGAATAGTTTACTCAGTCAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3754

Protein Analysis

713

Amino Acids

80.49

Weight (kDa)

6.24

Isoelectric Point (pI)

45.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3754 PF12576 478 - 608 1.8e-32 Protein of unknown function (DUF3754)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 210, 636
Acc36I ACCTGC 1 cut(s) 1345
AccBSI CCGCTC 1 cut(s) 38
AccI GTMKAC 2 cut(s) 853, 1454
AccII CGCG 1 cut(s) 1929
AccIII TCCGGA 1 cut(s) 1540
AciI CCGC 5 cut(s) 36, 185, 215, 823, 1929
AclI AACGTT 1 cut(s) 2113
AclWI GGATC 2 cut(s) 1274, 1551
AcsI RAATTY 4 cut(s) 576, 1028, 1161, 1652
AcuI CTGAAG 5 cut(s) 555, 606, 959, 1479, 1503
AcyI GRCGYC 3 cut(s) 207, 327, 633
AdeI CACNNNGTG 1 cut(s) 1322
AfaI GTAC 6 cut(s) 293, 479, 878, 990, 1057, 1480
AfeI AGCGCT 1 cut(s) 668
AfiI CCNNNNNNNGG 5 cut(s) 144, 1188, 1423, 1540, 1764
AflII CTTAAG 2 cut(s) 191, 1195
AgsI TTSAA 8 cut(s) 78, 405, 494, 704, 866, 964, 1381, 1988
AjnI CCWGG 3 cut(s) 281, 1419, 1757
Alw21I GWGCWC 1 cut(s) 423
Alw26I GTCTC 6 cut(s) 225, 307, 342, 1006, 1327, 1935
AlwI GGATC 2 cut(s) 1274, 1551
Aor13HI TCCGGA 1 cut(s) 1540
Aor51HI AGCGCT 1 cut(s) 668
AoxI GGCC 2 cut(s) 309, 1292
ApeKI GCWGC 5 cut(s) 316, 679, 1130, 1427, 2036
ApoI RAATTY 4 cut(s) 576, 1028, 1161, 1652
ArsI GACNNNNNNTTYG 2 cut(s) 683, 715
AspA2I CCTAGG 1 cut(s) 143
AspLEI GCGC 4 cut(s) 184, 669, 1602, 1627
AspS9I GGNCC 2 cut(s) 1178, 1293
AsuC2I CCSGG 1 cut(s) 299
AsuHPI GGTGA 4 cut(s) 16, 25, 106, 705
AsuII TTCGAA 2 cut(s) 266, 335
AvaII GGWCC 1 cut(s) 1178
AvrII CCTAGG 1 cut(s) 143
BanII GRGCYC 1 cut(s) 524
BbsI GAAGAC 2 cut(s) 246, 1388
Bbv12I GWGCWC 1 cut(s) 423
BbvI GCAGC 5 cut(s) 303, 691, 1142, 1439, 2023
BccI CCATC 4 cut(s) 242, 1304, 1510, 2048
BceAI ACGGC 5 cut(s) 45, 340, 373, 632, 1660
BcgI CGANNNNNNTGC 2 cut(s) 1034, 1068
BciT130I CCWGG 3 cut(s) 283, 1421, 1759
BciVI GTATCC 1 cut(s) 1739
BcnI CCSGG 1 cut(s) 299
BcoDI GTCTC 6 cut(s) 225, 307, 342, 1006, 1327, 1935
BfaI CTAG 4 cut(s) 144, 320, 872, 1812
BfmI CTRYAG 1 cut(s) 2037
BfoI RGCGCY 3 cut(s) 185, 670, 1628
BfrI CTTAAG 2 cut(s) 191, 1195
BfuAI ACCTGC 1 cut(s) 1345
BfuI GTATCC 1 cut(s) 1739
BglI GCCNNNNNGGC 1 cut(s) 1880
BisI GCNGC 6 cut(s) 185, 317, 680, 1131, 1428, 2037
BlnI CCTAGG 1 cut(s) 143
BlpI GCTNAGC 1 cut(s) 417
BlsI GCNGC 6 cut(s) 186, 318, 681, 1132, 1429, 2038
BmcAI AGTACT 1 cut(s) 1480
Bme1390I CCNGG 4 cut(s) 283, 299, 1421, 1759
Bme18I GGWCC 1 cut(s) 1178
BmgT120I GGNCC 2 cut(s) 1178, 1293
BmiI GGNNCC 2 cut(s) 279, 1180
BmrFI CCNGG 4 cut(s) 283, 299, 1421, 1759
BmrI ACTGGG 2 cut(s) 1166, 2036
BmsI GCATC 3 cut(s) 1249, 1255, 1834
BmuI ACTGGG 2 cut(s) 1166, 2036
BpiI GAAGAC 2 cut(s) 246, 1388
BpmI CTGGAG 1 cut(s) 1403
Bpu10I CCTNAGC 3 cut(s) 627, 1807, 1905
Bpu1102I GCTNAGC 1 cut(s) 417
Bpu14I TTCGAA 2 cut(s) 266, 335
BpuMI CCSGG 1 cut(s) 299
BsaBI GATNNNNATC 2 cut(s) 252, 1767
BsaHI GRCGYC 3 cut(s) 207, 327, 633
BsaI GGTCTC 3 cut(s) 307, 342, 1327
BsaJI CCNNGG 7 cut(s) 143, 281, 306, 471, 716, 1183, 1758
BsaWI WCCGGW 2 cut(s) 274, 1540
Bsc4I CCNNNNNNNGG 5 cut(s) 144, 1188, 1423, 1540, 1764
Bse118I RCCGGY 1 cut(s) 116
Bse1I ACTGG 5 cut(s) 788, 1172, 1550, 1994, 2031
Bse3DI GCAATG 2 cut(s) 510, 1608
Bse8I GATNNNNATC 2 cut(s) 252, 1767
BseAI TCCGGA 1 cut(s) 1540
BseBI CCWGG 3 cut(s) 283, 1421, 1759
BseDI CCNNGG 7 cut(s) 143, 281, 306, 471, 716, 1183, 1758
BseGI GGATG 2 cut(s) 294, 1849
BseJI GATNNNNATC 2 cut(s) 252, 1767
BseLI CCNNNNNNNGG 5 cut(s) 144, 1188, 1423, 1540, 1764
BseMI GCAATG 2 cut(s) 510, 1608
BseMII CTCAG 6 cut(s) 408, 710, 762, 812, 945, 1333
BseNI ACTGG 5 cut(s) 788, 1172, 1550, 1994, 2031
BseXI GCAGC 5 cut(s) 303, 691, 1142, 1439, 2023
BseYI CCCAGC 1 cut(s) 1084
Bsh1236I CGCG 1 cut(s) 1929
BshFI GGCC 2 cut(s) 311, 1294
BsiHKAI GWGCWC 1 cut(s) 423
BsiSI CCGG 4 cut(s) 117, 275, 299, 1541
BslFI GGGAC 1 cut(s) 197
BslI CCNNNNNNNGG 5 cut(s) 144, 1188, 1423, 1540, 1764
BsmAI GTCTC 6 cut(s) 225, 307, 342, 1006, 1327, 1935
BsmBI CGTCTC 1 cut(s) 225
BsmFI GGGAC 1 cut(s) 197
BsnI GGCC 2 cut(s) 311, 1294
Bso31I GGTCTC 3 cut(s) 307, 342, 1327
Bsp119I TTCGAA 2 cut(s) 266, 335
Bsp1286I GDGCHC 2 cut(s) 423, 524
Bsp13I TCCGGA 1 cut(s) 1540
Bsp143I GATC 5 cut(s) 253, 1078, 1093, 1279, 1543
Bsp1720I GCTNAGC 1 cut(s) 417
Bsp19I CCATGG 1 cut(s) 471
BspACI CCGC 5 cut(s) 36, 185, 215, 823, 1929
BspANI GGCC 2 cut(s) 311, 1294
BspCNI CTCAG 7 cut(s) 409, 709, 761, 811, 946, 1332, 2142
BspEI TCCGGA 1 cut(s) 1540
BspFNI CGCG 1 cut(s) 1929
BspLI GGNNCC 2 cut(s) 279, 1180
BspMAI CTGCAG 1 cut(s) 2041
BspMI ACCTGC 1 cut(s) 1345
BspPI GGATC 2 cut(s) 1274, 1551
BspQI GCTCTTC 2 cut(s) 112, 978
BspT104I TTCGAA 2 cut(s) 266, 335
BspTI CTTAAG 2 cut(s) 191, 1195
BspTNI GGTCTC 3 cut(s) 307, 342, 1327
BsrBI CCGCTC 1 cut(s) 38
BsrDI GCAATG 2 cut(s) 510, 1608
BsrFI RCCGGY 1 cut(s) 116
BsrI ACTGG 5 cut(s) 788, 1172, 1550, 1994, 2031
BssAI RCCGGY 1 cut(s) 116
BssECI CCNNGG 7 cut(s) 143, 281, 306, 471, 716, 1183, 1758
BssMI GATC 5 cut(s) 253, 1078, 1093, 1279, 1543
BssNAI GTATAC 1 cut(s) 1455
BssNI GRCGYC 3 cut(s) 207, 327, 633
BssT1I CCWWGG 4 cut(s) 143, 306, 471, 716
Bst1107I GTATAC 1 cut(s) 1455
Bst2UI CCWGG 3 cut(s) 283, 1421, 1759
Bst4CI ACNGT 3 cut(s) 885, 1355, 1597
Bst6I CTCTTC 3 cut(s) 112, 489, 978
BstACI GRCGYC 3 cut(s) 207, 327, 633
BstAFI CTTAAG 2 cut(s) 191, 1195
BstBI TTCGAA 2 cut(s) 266, 335
BstC8I GCNNGC 7 cut(s) 103, 447, 486, 1047, 1128, 2034, 2073
BstDSI CCRYGG 2 cut(s) 471, 1183
BstENI CCTNNNNNAGG 2 cut(s) 1421, 1762
BstF5I GGATG 2 cut(s) 294, 1849
BstFNI CGCG 1 cut(s) 1929
BstH2I RGCGCY 3 cut(s) 185, 670, 1628
BstHHI GCGC 4 cut(s) 184, 669, 1602, 1627
BstKTI GATC 5 cut(s) 256, 1081, 1096, 1282, 1546
BstMAI GTCTC 6 cut(s) 225, 307, 342, 1006, 1327, 1935
BstMBI GATC 5 cut(s) 253, 1078, 1093, 1279, 1543
BstMWI GCNNNNNNNGC 4 cut(s) 1139, 1631, 1709, 1880
BstNI CCWGG 3 cut(s) 283, 1421, 1759
BstNSI RCATGY 1 cut(s) 1950
BstSCI CCNGG 4 cut(s) 281, 297, 1419, 1757
BstSFI CTRYAG 1 cut(s) 2037
BstUI CGCG 1 cut(s) 1929
BstV1I GCAGC 5 cut(s) 303, 691, 1142, 1439, 2023
BstV2I GAAGAC 2 cut(s) 246, 1388
BstX2I RGATCY 1 cut(s) 1543
BstXI CCANNNNNNTGG 1 cut(s) 472
BstYI RGATCY 1 cut(s) 1543
BstZ17I GTATAC 1 cut(s) 1455
BsuI GTATCC 1 cut(s) 1739
BsuRI GGCC 2 cut(s) 311, 1294
BtgI CCRYGG 2 cut(s) 471, 1183
BtgZI GCGATG 1 cut(s) 375
BtsCI GGATG 2 cut(s) 294, 1849
BtsI GCAGTG 1 cut(s) 1707
BtsIMutI CAGTG 2 cut(s) 1327, 1707
BveI ACCTGC 1 cut(s) 1345
Cac8I GCNNGC 7 cut(s) 103, 447, 486, 1047, 1128, 2034, 2073
CfoI GCGC 4 cut(s) 184, 669, 1602, 1627
Cfr10I RCCGGY 1 cut(s) 116
Cfr13I GGNCC 2 cut(s) 1178, 1293
CseI GACGC 4 cut(s) 170, 316, 701, 1798
Csp6I GTAC 6 cut(s) 292, 478, 877, 989, 1056, 1479
CviAII CATG 7 cut(s) 413, 472, 676, 1212, 1344, 1947, 2082
CviQI GTAC 6 cut(s) 292, 478, 877, 989, 1056, 1479
DpnI GATC 5 cut(s) 255, 1080, 1095, 1281, 1545
DpnII GATC 5 cut(s) 253, 1078, 1093, 1279, 1543
DraI TTTAAA 1 cut(s) 1975
DraIII CACNNNGTG 1 cut(s) 1322
Eam1104I CTCTTC 3 cut(s) 112, 489, 978
EarI CTCTTC 3 cut(s) 112, 489, 978
Eco130I CCWWGG 4 cut(s) 143, 306, 471, 716
Eco24I GRGCYC 1 cut(s) 524
Eco31I GGTCTC 3 cut(s) 307, 342, 1327
Eco32I GATATC 1 cut(s) 1314
Eco47I GGWCC 1 cut(s) 1178
Eco47III AGCGCT 1 cut(s) 668
Eco57I CTGAAG 5 cut(s) 555, 606, 959, 1479, 1503
EcoNI CCTNNNNNAGG 2 cut(s) 1421, 1762
EcoRI GAATTC 1 cut(s) 1028
EcoRII CCWGG 3 cut(s) 281, 1419, 1757
EcoRV GATATC 1 cut(s) 1314
EcoT14I CCWWGG 4 cut(s) 143, 306, 471, 716
EcoT38I GRGCYC 1 cut(s) 524
ErhI CCWWGG 4 cut(s) 143, 306, 471, 716
Esp3I CGTCTC 1 cut(s) 225
FaeI CATG 7 cut(s) 416, 475, 679, 1215, 1347, 1950, 2085
FalI AAGNNNNNCTT 4 cut(s) 171, 203, 957, 989
FaqI GGGAC 1 cut(s) 197
FatI CATG 7 cut(s) 412, 471, 675, 1211, 1343, 1946, 2081
FauI CCCGC 3 cut(s) 222, 816, 1922
FauNDI CATATG 2 cut(s) 1328, 2095
FblI GTMKAC 2 cut(s) 853, 1454
Fnu4HI GCNGC 6 cut(s) 185, 317, 680, 1131, 1428, 2037
FokI GGATG 2 cut(s) 281, 1856
FriOI GRGCYC 1 cut(s) 524
Fsp4HI GCNGC 6 cut(s) 185, 317, 680, 1131, 1428, 2037
FspBI CTAG 4 cut(s) 144, 320, 872, 1812
GlaI GCGC 4 cut(s) 183, 668, 1601, 1626
GluI GCNGC 6 cut(s) 185, 317, 680, 1131, 1428, 2037
GsaI CCCAGC 1 cut(s) 1088
GsuI CTGGAG 1 cut(s) 1403
HaeII RGCGCY 3 cut(s) 185, 670, 1628
HaeIII GGCC 2 cut(s) 311, 1294
HapII CCGG 4 cut(s) 117, 275, 299, 1541
HgaI GACGC 4 cut(s) 170, 316, 701, 1798
HhaI GCGC 4 cut(s) 184, 669, 1602, 1627
Hin1I GRCGYC 3 cut(s) 207, 327, 633
Hin1II CATG 7 cut(s) 416, 475, 679, 1215, 1347, 1950, 2085
Hin6I GCGC 4 cut(s) 182, 667, 1600, 1625
HinP1I GCGC 4 cut(s) 182, 667, 1600, 1625
HincII GTYRAC 4 cut(s) 732, 781, 1783, 1999
HindII GTYRAC 4 cut(s) 732, 781, 1783, 1999
HindIII AAGCTT 1 cut(s) 1916
HpaII CCGG 4 cut(s) 117, 275, 299, 1541
HphI GGTGA 4 cut(s) 16, 25, 106, 705
Hpy166II GTNNAC 8 cut(s) 169, 732, 781, 854, 1455, 1783, 1999, 2126
Hpy188I TCNGA 9 cut(s) 125, 460, 526, 751, 978, 1075, 1105, 1279, 1852
Hpy188III TCNNGA 7 cut(s) 347, 434, 940, 1436, 1541, 1691, 2135
Hpy8I GTNNAC 8 cut(s) 169, 732, 781, 854, 1455, 1783, 1999, 2126
Hpy99I CGWCG 5 cut(s) 66, 164, 209, 212, 332
HpyAV CCTTC 9 cut(s) 84, 530, 784, 841, 1151, 1182, 1457, 1904, 2052
HpyCH4III ACNGT 3 cut(s) 885, 1355, 1597
HpyCH4IV ACGT 3 cut(s) 207, 633, 2113
HpyF10VI GCNNNNNNNGC 4 cut(s) 1139, 1631, 1709, 1880
HpySE526I ACGT 3 cut(s) 207, 633, 2113
Hsp92I GRCGYC 3 cut(s) 207, 327, 633
Hsp92II CATG 7 cut(s) 416, 475, 679, 1215, 1347, 1950, 2085
HspAI GCGC 4 cut(s) 182, 667, 1600, 1625
Kpn2I TCCGGA 1 cut(s) 1540
Kzo9I GATC 5 cut(s) 253, 1078, 1093, 1279, 1543
LguI GCTCTTC 2 cut(s) 112, 978
LmnI GCTCC 2 cut(s) 370, 942
Lsp1109I GCAGC 5 cut(s) 303, 691, 1142, 1439, 2023
LweI GCATC 3 cut(s) 1249, 1255, 1834
MaeI CTAG 4 cut(s) 144, 320, 872, 1812
MaeII ACGT 3 cut(s) 207, 633, 2113
MaeIII GTNAC 1 cut(s) 1322
MalI GATC 5 cut(s) 255, 1080, 1095, 1281, 1545
MbiI CCGCTC 1 cut(s) 38
MboI GATC 5 cut(s) 253, 1078, 1093, 1279, 1543
MfeI CAATTG 2 cut(s) 489, 2007
MflI RGATCY 1 cut(s) 1543
MhlI GDGCHC 2 cut(s) 423, 524
MluCI AATT 8 cut(s) 405, 489, 576, 1028, 1161, 1439, 1652, 2007
MlyI GAGTC 1 cut(s) 965
MmeI TCCRAC 4 cut(s) 489, 1156, 1918, 2096
MroI TCCGGA 1 cut(s) 1540
MseI TTAA 9 cut(s) 192, 594, 725, 743, 1196, 1514, 1659, 1974, 2106
MspCI CTTAAG 2 cut(s) 191, 1195
MspI CCGG 4 cut(s) 117, 275, 299, 1541
MspR9I CCNGG 4 cut(s) 283, 299, 1421, 1759
MunI CAATTG 2 cut(s) 489, 2007
MvaI CCWGG 3 cut(s) 283, 1421, 1759
MvnI CGCG 1 cut(s) 1929
MwoI GCNNNNNNNGC 4 cut(s) 1139, 1631, 1709, 1880
NciI CCSGG 1 cut(s) 299
NcoI CCATGG 1 cut(s) 471
NdeI CATATG 2 cut(s) 1328, 2095
NdeII GATC 5 cut(s) 253, 1078, 1093, 1279, 1543
NlaIII CATG 7 cut(s) 416, 475, 679, 1215, 1347, 1950, 2085
NlaIV GGNNCC 2 cut(s) 279, 1180
NmeAIII GCCGAG 1 cut(s) 337
NmuCI GTSAC 1 cut(s) 1322
NspI RCATGY 1 cut(s) 1950
NspV TTCGAA 2 cut(s) 266, 335
PciSI GCTCTTC 2 cut(s) 112, 978
PcsI WCGNNNNNNNCGW 1 cut(s) 240
PfeI GAWTC 9 cut(s) 271, 337, 401, 430, 461, 527, 540, 903, 2099
PflFI GACNNNGTC 2 cut(s) 209, 326
PfoI TCCNGGA 1 cut(s) 1419
PkrI GCNGC 6 cut(s) 186, 318, 681, 1132, 1429, 2038
PleI GAGTC 1 cut(s) 964
PpsI GAGTC 1 cut(s) 964
Psp1406I AACGTT 1 cut(s) 2113
Psp6I CCWGG 3 cut(s) 281, 1419, 1757
PspFI CCCAGC 1 cut(s) 1084
PspGI CCWGG 3 cut(s) 281, 1419, 1757
PspN4I GGNNCC 2 cut(s) 279, 1180
PspPI GGNCC 2 cut(s) 1178, 1293
PstI CTGCAG 1 cut(s) 2041
PsuI RGATCY 1 cut(s) 1543
PsyI GACNNNGTC 2 cut(s) 209, 326
RsaI GTAC 6 cut(s) 293, 479, 878, 990, 1057, 1480
RsaNI GTAC 6 cut(s) 292, 478, 877, 989, 1056, 1479
SapI GCTCTTC 2 cut(s) 112, 978
SaqAI TTAA 9 cut(s) 192, 594, 725, 743, 1196, 1514, 1659, 1974, 2106
SatI GCNGC 6 cut(s) 185, 317, 680, 1131, 1428, 2037
Sau3AI GATC 5 cut(s) 253, 1078, 1093, 1279, 1543
Sau96I GGNCC 2 cut(s) 1178, 1293
ScaI AGTACT 1 cut(s) 1480
SchI GAGTC 1 cut(s) 965
ScrFI CCNGG 4 cut(s) 283, 299, 1421, 1759
SduI GDGCHC 2 cut(s) 423, 524
SfaNI GCATC 3 cut(s) 1249, 1255, 1834
SfcI CTRYAG 1 cut(s) 2037
SfuI TTCGAA 2 cut(s) 266, 335
SinI GGWCC 1 cut(s) 1178
SmlI CTYRAG 2 cut(s) 191, 1195
SmoI CTYRAG 2 cut(s) 191, 1195
Sse9I AATT 8 cut(s) 405, 489, 576, 1028, 1161, 1439, 1652, 2007
SsiI CCGC 5 cut(s) 36, 185, 215, 823, 1929
SspMI CTAG 4 cut(s) 144, 320, 872, 1812
StyD4I CCNGG 4 cut(s) 281, 297, 1419, 1757
StyI CCWWGG 4 cut(s) 143, 306, 471, 716
TaaI ACNGT 3 cut(s) 885, 1355, 1597
TaiI ACGT 3 cut(s) 210, 636, 2116
TaqI TCGA 6 cut(s) 266, 335, 346, 585, 920, 2087
TasI AATT 8 cut(s) 405, 489, 576, 1028, 1161, 1439, 1652, 2007
TatI WGTACW 4 cut(s) 291, 477, 876, 1478
TauI GCSGC 1 cut(s) 187
TfiI GAWTC 9 cut(s) 271, 337, 401, 430, 461, 527, 540, 903, 2099
Tru1I TTAA 9 cut(s) 192, 594, 725, 743, 1196, 1514, 1659, 1974, 2106
Tru9I TTAA 9 cut(s) 192, 594, 725, 743, 1196, 1514, 1659, 1974, 2106
TscAI CASTG 2 cut(s) 1327, 1714
TseFI GTSAC 1 cut(s) 1322
TseI GCWGC 5 cut(s) 316, 679, 1130, 1427, 2036
Tsp45I GTSAC 1 cut(s) 1322
TspGWI ACGGA 2 cut(s) 570, 1571
TspRI CASTG 2 cut(s) 1327, 1714
Tth111I GACNNNGTC 2 cut(s) 209, 326
Vha464I CTTAAG 2 cut(s) 191, 1195
VpaK11BI GGWCC 1 cut(s) 1178
XagI CCTNNNNNAGG 2 cut(s) 1421, 1762
XapI RAATTY 4 cut(s) 576, 1028, 1161, 1652
XceI RCATGY 1 cut(s) 1950
XmaJI CCTAGG 1 cut(s) 143
XmiI GTMKAC 2 cut(s) 853, 1454
XspI CTAG 4 cut(s) 144, 320, 872, 1812
ZraI GACGTC 2 cut(s) 208, 634
ZrmI AGTACT 1 cut(s) 1480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.