MD17G1162400.v1.1

Protein of unknown function (DUF3754)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0005827
Physical Location & Seq
Forward (+)
16059234 .. 16066004
6771 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1162400.v1.1.491

Sequence Viewer

Length: 2160 bp
ATGTCAGCTTCTTGGCACGCTCTCACCGGCCTCGGAGCTCTCTCCGTAACCGTAAAACAGCGCCGTCTAATTCCTTCACCACCATCAGTTGCTCACTGCTCTCTCGCCAGCTTCCAGGAACTGTCGACGGAGCCTCCGCGAATCCAAACCACCGGCGCCGCTATAAGCTTGAAAGACGCCCGCAAGACCGAGAAGCAAAACGACGTCGTTCCCGATCATAACCAAGGCGAAGAAGAAGAAGAAGACCATGGAATTTCCAAAATCCCGGTCCCCAGGCAGAAGTACATCCCCGTTCCCAAGGCCGAGCTTCTCGACGGCGTCGTTTCGAACTTGTTCAAGGACAGCAACGGCGAAGACGACGATGAATTGCAGAGCTTTCTGCTTCTCTCTTCGTGCTTGGACTCCATTCTTCACGCCGAGCACAAGGGAATCTTGGAAGAAATGCGAGCTGATTATTTCAAATCGAATTCCGCGGAGAACAAGCAAAATGGTGATTTGGTTGATGGGCAGAGCTCGGATTCTGCTAATGGGAGTGTCATCAATGGAGTTGAGAGCATTGAGGCAAATGAGGATGAAAAAAATGAAGGTGGAAACGCAATGCCGCAGTTAGATTATGTTTTGGAGTTGCGAAAACTCTTGGGTTCTCCGGCTAAGAACGTTGAGAGAGTTGCTGTTTCCACTCGTTTCCAGCGTTCTTTCATGCAACTTCTTAATGATGCTCAGTTTGAAGAACTATCCGAAAGGGATTTAATGCTGACTTCTGCATTGAACACAGATTATCTCCTTACTTTGCCCATATATGTCGACTGGAAGAAGGCATCTGAGTCCAATGCAATTATATTCAGGCGAGGATATGCAACAGAGAGGGAGAAGGGTCTACTACTTGTAGAAAAACTGGAGTACATACAGTCCAAAATTCTACAGGGAACCTTCTTCATCGTATCAAAACCGTTGGCGAAATTTGGGAGCTGGATAGTTGAGGCATTCCAAGTTGCTTGCGAAACACCAGAAGTACAAGACTTGACTAAAAGAATGAAGCTTTGGATTAAGGAATTGTCTGTTTTCCAGCAAGCATTTCGTTATAGCGAACAAACTTTTGATGATCTACTGGGAGTAGAGCAACTATCAAATAAAGACCTCCCAATTTGGCTGGCGGCACAGAGGGCAGTATCTCGTTATGAAGGACGTTTATCTTCAGTTGGACCCCGTGGAAGGCTCCTAAAGAAGTTGCTTTCATGGATTGGACTTATTTCCCCTACACCAGAAACACCATTTGAGTTGGATGGCGATACTAATGATTCTGACCCTTATGCAAGGCCGATCTTCTTATCAAGGATATCACTTAGTGACATATGGAGGCCTGCTACTAGGAAATACTGTGGAAATGATATTTGGAAAATGCTAAAAACTTCTGTTTCTATTCTCTTCTCACAGTCCATCCTCCAGGAGGCAGCATTTCAAGAATTAATTTTGCTTTATACCCAAGAAGTAGATGGCAGGAATGCTGACTACAATGCTACTGTTCCATCGTTGCAGTTAAAGATTTATGAGAAAATTCCCATTCCGGATCTACCAGTTATCTTTCCCCACAAAAAGCTATCTTTCCGTATCATAGACACGGTGCGCTTGGACATCGCCTCAATACTAGGGCTTTCGGCATTCTTCATAAACTACAAATTTTTTAATGTCTTATCTTCCCCGTCAGCAATAGTTCTTGATGTGGTTGCAATCAGCGCTCTTATAATTTATGTGTCCCGTGTGGTTTTGGGATACAAACAGACGTGGGATAGGTATCAACTTCTAGTCAACACGACGCTTTATGAGAAAACCTTGGCAAGTGGATTTGGCTCAGTTCATTTTCTTCTGGATGCTTCTGAACAACAACAATACAAGGAGGCCATTTTGGCATATGCAATCCTTCTCAAAGCAAACAAGGGGAAGCTCACGTGTTGCCAGAGTGTTGGAGACGACTGTGAAAGATTTATGTATGATGTGTTTAAACTAAAGGTTGAAATGCCAGTTGACAAGGCAGTTGCTACATTATTAAGGCTGGGGTTAGCTACAGAAACTCCTATTGACGGAAGGATTAGTTTGGAGGCCATTTCCTGTTCAAAGGCACACAATTCCCTGAAAGAGCGTTGGAATAGTTTGCTCAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3754

Protein Analysis

720

Amino Acids

80.68

Weight (kDa)

5.41

Isoelectric Point (pI)

43.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3754 PF12576 486 - 616 3.4e-32 Protein of unknown function (DUF3754)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1742
AatII GACGTC 1 cut(s) 207
AccB1I GGYRCC 1 cut(s) 155
AccI GTMKAC 3 cut(s) 125, 804, 877
AccII CGCG 2 cut(s) 139, 473
AccIII TCCGGA 1 cut(s) 1564
AciI CCGC 7 cut(s) 137, 159, 181, 471, 473, 602, 1154
AclI AACGTT 1 cut(s) 657
AclWI GGATC 1 cut(s) 1575
AcsI RAATTY 6 cut(s) 252, 466, 915, 959, 1554, 1676
AcuI CTGAAG 1 cut(s) 1179
AcvI CACGTG 1 cut(s) 1947
AcyI GRCGYC 4 cut(s) 156, 177, 204, 318
AdeI CACNNNGTG 1 cut(s) 1346
AfaI GTAC 3 cut(s) 284, 902, 1014
AfeI AGCGCT 1 cut(s) 1735
AfiI CCNNNNNNNGG 3 cut(s) 1212, 1447, 2078
AflIII ACRYGT 1 cut(s) 1946
AgsI TTSAA 8 cut(s) 172, 337, 460, 728, 769, 1460, 2012, 2112
AjiI CACGTC 1 cut(s) 1782
AjnI CCWGG 3 cut(s) 114, 272, 1443
AjuI GAANNNNNNNTTGG 4 cut(s) 1024, 1056, 1375, 1407
Alw21I GWGCWC 3 cut(s) 40, 423, 515
Alw26I GTCTC 1 cut(s) 1959
AlwI GGATC 1 cut(s) 1575
AlwNI CAGNNNCTG 1 cut(s) 121
Aor13HI TCCGGA 1 cut(s) 1564
Aor51HI AGCGCT 1 cut(s) 1735
AoxI GGCC 6 cut(s) 28, 300, 1316, 1358, 1896, 2097
ApeKI GCWGC 1 cut(s) 1451
ApoI RAATTY 6 cut(s) 252, 466, 915, 959, 1554, 1676
ArsI GACNNNNNNTTYG 2 cut(s) 252, 284
AseI ATTAAT 1 cut(s) 1466
Asp700I GAANNNNTTC 1 cut(s) 332
AspLEI GCGC 4 cut(s) 63, 158, 1626, 1736
AspS9I GGNCC 2 cut(s) 268, 1202
AsuC2I CCSGG 1 cut(s) 266
AsuHPI GGTGA 3 cut(s) 16, 69, 503
AsuII TTCGAA 1 cut(s) 326
AvaII GGWCC 2 cut(s) 268, 1202
BanI GGYRCC 1 cut(s) 155
BanII GRGCYC 2 cut(s) 40, 515
BbrPI CACGTG 1 cut(s) 1947
BbsI GAAGAC 2 cut(s) 249, 360
Bbv12I GWGCWC 3 cut(s) 40, 423, 515
BbvI GCAGC 1 cut(s) 1463
BccI CCATC 6 cut(s) 91, 497, 1277, 1445, 1487, 1534
BceAI ACGGC 3 cut(s) 48, 331, 364
BcgI CGANNNNNNTGC 2 cut(s) 1058, 1092
BciT130I CCWGG 3 cut(s) 116, 274, 1445
BciVI GTATCC 1 cut(s) 1763
BcnI CCSGG 1 cut(s) 266
BcoDI GTCTC 1 cut(s) 1959
BfaI CTAG 3 cut(s) 1368, 1646, 1802
BfmI CTRYAG 2 cut(s) 920, 2061
BfoI RGCGCY 3 cut(s) 64, 159, 1737
BfuI GTATCC 1 cut(s) 1763
BglI GCCNNNNNGGC 1 cut(s) 1904
BisI GCNGC 4 cut(s) 159, 602, 1155, 1452
BlsI GCNGC 4 cut(s) 160, 603, 1156, 1453
Bme1390I CCNGG 4 cut(s) 116, 266, 274, 1445
Bme18I GGWCC 2 cut(s) 268, 1202
BmgBI CACGTC 1 cut(s) 1782
BmgT120I GGNCC 2 cut(s) 268, 1202
BmiI GGNNCC 6 cut(s) 132, 157, 270, 928, 1204, 1217
BmrFI CCNGG 4 cut(s) 116, 266, 274, 1445
BmrI ACTGGG 1 cut(s) 1118
BmsI GCATC 3 cut(s) 706, 827, 1858
BmuI ACTGGG 1 cut(s) 1118
BpiI GAAGAC 2 cut(s) 249, 360
BpmI CTGGAG 2 cut(s) 917, 1427
Bpu14I TTCGAA 1 cut(s) 326
BpuMI CCSGG 1 cut(s) 266
BsaAI YACGTR 1 cut(s) 1947
BsaBI GATNNNNATC 1 cut(s) 1791
BsaHI GRCGYC 4 cut(s) 156, 177, 204, 318
BsaJI CCNNGG 8 cut(s) 31, 223, 247, 272, 297, 471, 1207, 1830
BsaWI WCCGGW 1 cut(s) 1564
BsaXI ACNNNNNCTCC 4 cut(s) 118, 148, 2053, 2083
Bsc4I CCNNNNNNNGG 3 cut(s) 1212, 1447, 2078
Bse118I RCCGGY 2 cut(s) 26, 152
Bse1I ACTGG 5 cut(s) 812, 900, 1113, 1574, 2018
Bse3DI GCAATG 1 cut(s) 603
Bse8I GATNNNNATC 1 cut(s) 1791
BseAI TCCGGA 1 cut(s) 1564
BseBI CCWGG 3 cut(s) 116, 274, 1445
BseDI CCNNGG 8 cut(s) 31, 223, 247, 272, 297, 471, 1207, 1830
BseGI GGATG 5 cut(s) 285, 577, 1288, 1437, 1873
BseJI GATNNNNATC 1 cut(s) 1791
BseLI CCNNNNNNNGG 3 cut(s) 1212, 1447, 2078
BseMI GCAATG 1 cut(s) 603
BseMII CTCAG 3 cut(s) 734, 813, 1863
BseNI ACTGG 5 cut(s) 812, 900, 1113, 1574, 2018
BseXI GCAGC 1 cut(s) 1463
BseYI CCCAGC 1 cut(s) 2050
Bsh1236I CGCG 2 cut(s) 139, 473
BshFI GGCC 6 cut(s) 30, 302, 1318, 1360, 1898, 2099
BshNI GGYRCC 1 cut(s) 155
BsiHKAI GWGCWC 3 cut(s) 40, 423, 515
BsiSI CCGG 5 cut(s) 27, 153, 266, 647, 1565
BslFI GGGAC 2 cut(s) 254, 1738
BslI CCNNNNNNNGG 3 cut(s) 1212, 1447, 2078
BsmAI GTCTC 1 cut(s) 1959
BsmBI CGTCTC 1 cut(s) 1959
BsmFI GGGAC 2 cut(s) 254, 1738
BsmI GAATGC 3 cut(s) 983, 1507, 1658
BsnI GGCC 6 cut(s) 30, 302, 1318, 1360, 1898, 2099
Bsp119I TTCGAA 1 cut(s) 326
Bsp1286I GDGCHC 3 cut(s) 40, 423, 515
Bsp13I TCCGGA 1 cut(s) 1564
Bsp143I GATC 4 cut(s) 214, 1102, 1320, 1567
Bsp19I CCATGG 1 cut(s) 247
BspACI CCGC 7 cut(s) 137, 159, 181, 471, 473, 602, 1154
BspANI GGCC 6 cut(s) 30, 302, 1318, 1360, 1898, 2099
BspCNI CTCAG 3 cut(s) 733, 814, 1862
BspEI TCCGGA 1 cut(s) 1564
BspFNI CGCG 2 cut(s) 139, 473
BspLI GGNNCC 6 cut(s) 132, 157, 270, 928, 1204, 1217
BspPI GGATC 1 cut(s) 1575
BspT104I TTCGAA 1 cut(s) 326
BspT107I GGYRCC 1 cut(s) 155
BsrDI GCAATG 1 cut(s) 603
BsrFI RCCGGY 2 cut(s) 26, 152
BsrI ACTGG 5 cut(s) 812, 900, 1113, 1574, 2018
BssAI RCCGGY 2 cut(s) 26, 152
BssECI CCNNGG 8 cut(s) 31, 223, 247, 272, 297, 471, 1207, 1830
BssMI GATC 4 cut(s) 214, 1102, 1320, 1567
BssNI GRCGYC 4 cut(s) 156, 177, 204, 318
BssT1I CCWWGG 4 cut(s) 223, 247, 297, 1830
Bst2UI CCWGG 3 cut(s) 116, 274, 1445
Bst4CI ACNGT 9 cut(s) 52, 123, 909, 951, 1379, 1434, 1522, 1621, 1973
Bst6I CTCTTC 2 cut(s) 394, 1430
BstACI GRCGYC 4 cut(s) 156, 177, 204, 318
BstBAI YACGTR 1 cut(s) 1947
BstBI TTCGAA 1 cut(s) 326
BstC8I GCNNGC 8 cut(s) 18, 109, 181, 447, 997, 1071, 1152, 1362
BstDEI CTNAG 6 cut(s) 651, 720, 822, 1343, 1849, 2153
BstDSI CCRYGG 3 cut(s) 247, 471, 1207
BstENI CCTNNNNNAGG 1 cut(s) 1445
BstF5I GGATG 5 cut(s) 285, 577, 1288, 1437, 1873
BstFNI CGCG 2 cut(s) 139, 473
BstH2I RGCGCY 3 cut(s) 64, 159, 1737
BstHHI GCGC 4 cut(s) 63, 158, 1626, 1736
BstKTI GATC 4 cut(s) 217, 1105, 1323, 1570
BstMAI GTCTC 1 cut(s) 1959
BstMBI GATC 4 cut(s) 214, 1102, 1320, 1567
BstMWI GCNNNNNNNGC 3 cut(s) 1163, 1733, 1904
BstNI CCWGG 3 cut(s) 116, 274, 1445
BstSCI CCNGG 4 cut(s) 114, 264, 272, 1443
BstSFI CTRYAG 2 cut(s) 920, 2061
BstUI CGCG 2 cut(s) 139, 473
BstV1I GCAGC 1 cut(s) 1463
BstV2I GAAGAC 2 cut(s) 249, 360
BstX2I RGATCY 1 cut(s) 1567
BstXI CCANNNNNNTGG 1 cut(s) 1961
BstYI RGATCY 1 cut(s) 1567
BsuI GTATCC 1 cut(s) 1763
BsuRI GGCC 6 cut(s) 30, 302, 1318, 1360, 1898, 2099
BtgI CCRYGG 3 cut(s) 247, 471, 1207
BtgZI GCGATG 1 cut(s) 1618
BtrI CACGTC 1 cut(s) 1782
BtsCI GGATG 5 cut(s) 285, 577, 1288, 1437, 1873
BtsI GCAGTG 1 cut(s) 94
BtsIMutI CAGTG 1 cut(s) 94
Cac8I GCNNGC 8 cut(s) 18, 109, 181, 447, 997, 1071, 1152, 1362
CaiI CAGNNNCTG 1 cut(s) 121
CfoI GCGC 4 cut(s) 63, 158, 1626, 1736
Cfr10I RCCGGY 2 cut(s) 26, 152
Cfr13I GGNCC 2 cut(s) 268, 1202
Cfr42I CCGCGG 1 cut(s) 474
CseI GACGC 3 cut(s) 185, 307, 1822
Csp6I GTAC 3 cut(s) 283, 901, 1013
CviAII CATG 3 cut(s) 248, 700, 1236
CviQI GTAC 3 cut(s) 283, 901, 1013
DdeI CTNAG 6 cut(s) 651, 720, 822, 1343, 1849, 2153
DinI GGCGCC 1 cut(s) 157
DpnI GATC 4 cut(s) 216, 1104, 1322, 1569
DpnII GATC 4 cut(s) 214, 1102, 1320, 1567
DraI TTTAAA 1 cut(s) 1999
DraIII CACNNNGTG 1 cut(s) 1346
Eam1104I CTCTTC 2 cut(s) 394, 1430
EarI CTCTTC 2 cut(s) 394, 1430
Ecl136II GAGCTC 2 cut(s) 38, 513
Eco130I CCWWGG 4 cut(s) 223, 247, 297, 1830
Eco147I AGGCCT 1 cut(s) 1360
Eco24I GRGCYC 2 cut(s) 40, 515
Eco32I GATATC 1 cut(s) 1338
Eco47I GGWCC 2 cut(s) 268, 1202
Eco47III AGCGCT 1 cut(s) 1735
Eco53kI GAGCTC 2 cut(s) 38, 513
Eco57I CTGAAG 1 cut(s) 1179
Eco72I CACGTG 1 cut(s) 1947
EcoICRI GAGCTC 2 cut(s) 38, 513
EcoNI CCTNNNNNAGG 1 cut(s) 1445
EcoRI GAATTC 1 cut(s) 466
EcoRII CCWGG 3 cut(s) 114, 272, 1443
EcoRV GATATC 1 cut(s) 1338
EcoT14I CCWWGG 4 cut(s) 223, 247, 297, 1830
EcoT38I GRGCYC 2 cut(s) 40, 515
EgeI GGCGCC 1 cut(s) 157
EheI GGCGCC 1 cut(s) 157
ErhI CCWWGG 4 cut(s) 223, 247, 297, 1830
Esp3I CGTCTC 1 cut(s) 1959
FaeI CATG 3 cut(s) 251, 703, 1239
FalI AAGNNNNNCTT 2 cut(s) 416, 448
FaqI GGGAC 2 cut(s) 254, 1738
FatI CATG 3 cut(s) 247, 699, 1235
FauI CCCGC 1 cut(s) 188
FauNDI CATATG 2 cut(s) 1352, 1909
FblI GTMKAC 3 cut(s) 125, 804, 877
Fnu4HI GCNGC 4 cut(s) 159, 602, 1155, 1452
FokI GGATG 5 cut(s) 272, 584, 1295, 1424, 1880
FriOI GRGCYC 2 cut(s) 40, 515
Fsp4HI GCNGC 4 cut(s) 159, 602, 1155, 1452
FspBI CTAG 3 cut(s) 1368, 1646, 1802
GlaI GCGC 4 cut(s) 62, 157, 1625, 1735
GluI GCNGC 4 cut(s) 159, 602, 1155, 1452
GsaI CCCAGC 1 cut(s) 2054
GsuI CTGGAG 2 cut(s) 917, 1427
HaeII RGCGCY 3 cut(s) 64, 159, 1737
HaeIII GGCC 6 cut(s) 30, 302, 1318, 1360, 1898, 2099
HapII CCGG 5 cut(s) 27, 153, 266, 647, 1565
HgaI GACGC 3 cut(s) 185, 307, 1822
HhaI GCGC 4 cut(s) 63, 158, 1626, 1736
Hin1I GRCGYC 4 cut(s) 156, 177, 204, 318
Hin1II CATG 3 cut(s) 251, 703, 1239
Hin6I GCGC 4 cut(s) 61, 156, 1624, 1734
HinP1I GCGC 4 cut(s) 61, 156, 1624, 1734
HincII GTYRAC 4 cut(s) 126, 805, 1807, 2023
HindII GTYRAC 4 cut(s) 126, 805, 1807, 2023
HindIII AAGCTT 2 cut(s) 166, 1037
HinfI GANTC 6 cut(s) 141, 401, 429, 518, 824, 1298
HpaII CCGG 5 cut(s) 27, 153, 266, 647, 1565
HphI GGTGA 3 cut(s) 16, 69, 503
Hpy166II GTNNAC 5 cut(s) 126, 805, 878, 1807, 2023
Hpy188I TCNGA 6 cut(s) 35, 517, 739, 823, 1303, 1876
Hpy188III TCNNGA 6 cut(s) 212, 311, 1460, 1565, 1715, 1865
Hpy8I GTNNAC 5 cut(s) 126, 805, 878, 1807, 2023
Hpy99I CGWCG 7 cut(s) 130, 206, 209, 317, 323, 362, 1816
HpyAV CCTTC 9 cut(s) 84, 578, 808, 865, 940, 1175, 1206, 1928, 2076
HpyCH4III ACNGT 9 cut(s) 52, 123, 909, 951, 1379, 1434, 1522, 1621, 1973
HpyCH4IV ACGT 5 cut(s) 204, 657, 1186, 1781, 1946
HpyCH4V TGCA 9 cut(s) 370, 703, 764, 833, 857, 1313, 1534, 1727, 1913
HpyF10VI GCNNNNNNNGC 3 cut(s) 1163, 1733, 1904
HpyF3I CTNAG 6 cut(s) 651, 720, 822, 1343, 1849, 2153
HpySE526I ACGT 5 cut(s) 204, 657, 1186, 1781, 1946
Hsp92I GRCGYC 4 cut(s) 156, 177, 204, 318
Hsp92II CATG 3 cut(s) 251, 703, 1239
HspAI GCGC 4 cut(s) 61, 156, 1624, 1734
KasI GGCGCC 1 cut(s) 155
Kpn2I TCCGGA 1 cut(s) 1564
KspI CCGCGG 1 cut(s) 474
Kzo9I GATC 4 cut(s) 214, 1102, 1320, 1567
LmnI GCTCC 4 cut(s) 35, 130, 966, 1221
Lsp1109I GCAGC 1 cut(s) 1463
LweI GCATC 3 cut(s) 706, 827, 1858
MaeI CTAG 3 cut(s) 1368, 1646, 1802
MaeII ACGT 5 cut(s) 204, 657, 1186, 1781, 1946
MaeIII GTNAC 2 cut(s) 46, 1346
MalI GATC 4 cut(s) 216, 1104, 1322, 1569
MboI GATC 4 cut(s) 214, 1102, 1320, 1567
MflI RGATCY 1 cut(s) 1567
MhlI GDGCHC 3 cut(s) 40, 423, 515
Mly113I GGCGCC 1 cut(s) 156
MlyI GAGTC 2 cut(s) 395, 833
MmeI TCCRAC 4 cut(s) 1180, 1260, 1942, 2120
MroI TCCGGA 1 cut(s) 1564
MroXI GAANNNNTTC 1 cut(s) 332
MseI TTAA 9 cut(s) 711, 749, 1047, 1466, 1538, 1683, 1998, 2045, 2158
MspA1I CMGCKG 1 cut(s) 473
MspI CCGG 5 cut(s) 27, 153, 266, 647, 1565
MspR9I CCNGG 4 cut(s) 116, 266, 274, 1445
MssI GTTTAAAC 1 cut(s) 1999
Mva1269I GAATGC 3 cut(s) 983, 1507, 1658
MvaI CCWGG 3 cut(s) 116, 274, 1445
MvnI CGCG 2 cut(s) 139, 473
MwoI GCNNNNNNNGC 3 cut(s) 1163, 1733, 1904
NarI GGCGCC 1 cut(s) 156
NciI CCSGG 1 cut(s) 266
NcoI CCATGG 1 cut(s) 247
NdeI CATATG 2 cut(s) 1352, 1909
NdeII GATC 4 cut(s) 214, 1102, 1320, 1567
NlaIII CATG 3 cut(s) 251, 703, 1239
NlaIV GGNNCC 6 cut(s) 132, 157, 270, 928, 1204, 1217
NmeAIII GCCGAG 2 cut(s) 328, 442
NmuCI GTSAC 1 cut(s) 1346
NspV TTCGAA 1 cut(s) 326
PceI AGGCCT 1 cut(s) 1360
PcsI WCGNNNNNNNCGW 4 cut(s) 210, 318, 354, 688
PctI GAATGC 3 cut(s) 983, 1507, 1658
PdmI GAANNNNTTC 1 cut(s) 332
PfeI GAWTC 4 cut(s) 141, 429, 518, 1298
PflFI GACNNNGTC 1 cut(s) 317
PfoI TCCNGGA 2 cut(s) 114, 1443
PkrI GCNGC 4 cut(s) 160, 603, 1156, 1453
PleI GAGTC 2 cut(s) 395, 832
PluTI GGCGCC 1 cut(s) 159
PmaCI CACGTG 1 cut(s) 1947
PmeI GTTTAAAC 1 cut(s) 1999
PmlI CACGTG 1 cut(s) 1947
PpsI GAGTC 2 cut(s) 395, 832
Ppu21I YACGTR 1 cut(s) 1947
PshBI ATTAAT 1 cut(s) 1466
PsiI TTATAA 1 cut(s) 1742
Psp124BI GAGCTC 2 cut(s) 40, 515
Psp1406I AACGTT 1 cut(s) 657
Psp6I CCWGG 3 cut(s) 114, 272, 1443
PspCI CACGTG 1 cut(s) 1947
PspFI CCCAGC 1 cut(s) 2050
PspGI CCWGG 3 cut(s) 114, 272, 1443
PspN4I GGNNCC 6 cut(s) 132, 157, 270, 928, 1204, 1217
PspPI GGNCC 2 cut(s) 268, 1202
PstNI CAGNNNCTG 1 cut(s) 121
PsuI RGATCY 1 cut(s) 1567
PsyI GACNNNGTC 1 cut(s) 317
RsaI GTAC 3 cut(s) 284, 902, 1014
RsaNI GTAC 3 cut(s) 283, 901, 1013
SacI GAGCTC 2 cut(s) 40, 515
SacII CCGCGG 1 cut(s) 474
SalI GTCGAC 2 cut(s) 124, 803
SaqAI TTAA 9 cut(s) 711, 749, 1047, 1466, 1538, 1683, 1998, 2045, 2158
SatI GCNGC 4 cut(s) 159, 602, 1155, 1452
Sau3AI GATC 4 cut(s) 214, 1102, 1320, 1567
Sau96I GGNCC 2 cut(s) 268, 1202
SchI GAGTC 2 cut(s) 395, 833
ScrFI CCNGG 4 cut(s) 116, 266, 274, 1445
SduI GDGCHC 3 cut(s) 40, 423, 515
SfaNI GCATC 3 cut(s) 706, 827, 1858
SfcI CTRYAG 2 cut(s) 920, 2061
SfoI GGCGCC 1 cut(s) 157
Sfr303I CCGCGG 1 cut(s) 474
SfuI TTCGAA 1 cut(s) 326
SgrAI CRCCGGYG 1 cut(s) 152
SgrBI CCGCGG 1 cut(s) 474
SinI GGWCC 2 cut(s) 268, 1202
SseBI AGGCCT 1 cut(s) 1360
SsiI CCGC 7 cut(s) 137, 159, 181, 471, 473, 602, 1154
SspDI GGCGCC 1 cut(s) 155
SspMI CTAG 3 cut(s) 1368, 1646, 1802
SstI GAGCTC 2 cut(s) 40, 515
StuI AGGCCT 1 cut(s) 1360
StyD4I CCNGG 4 cut(s) 114, 264, 272, 1443
StyI CCWWGG 4 cut(s) 223, 247, 297, 1830
TaaI ACNGT 9 cut(s) 52, 123, 909, 951, 1379, 1434, 1522, 1621, 1973
TaiI ACGT 5 cut(s) 207, 660, 1189, 1784, 1949
TaqI TCGA 5 cut(s) 125, 312, 326, 464, 804
TaqII GACCGA 1 cut(s) 203
TatI WGTACW 3 cut(s) 282, 900, 1012
TauI GCSGC 3 cut(s) 161, 604, 1157
TfiI GAWTC 4 cut(s) 141, 429, 518, 1298
Tru1I TTAA 9 cut(s) 711, 749, 1047, 1466, 1538, 1683, 1998, 2045, 2158
Tru9I TTAA 9 cut(s) 711, 749, 1047, 1466, 1538, 1683, 1998, 2045, 2158
TscAI CASTG 1 cut(s) 101
TseFI GTSAC 1 cut(s) 1346
TseI GCWGC 1 cut(s) 1451
Tsp45I GTSAC 1 cut(s) 1346
TspGWI ACGGA 4 cut(s) 34, 143, 1595, 2094
TspRI CASTG 1 cut(s) 101
Tth111I GACNNNGTC 1 cut(s) 317
VpaK11BI GGWCC 2 cut(s) 268, 1202
VspI ATTAAT 1 cut(s) 1466
XagI CCTNNNNNAGG 1 cut(s) 1445
XapI RAATTY 6 cut(s) 252, 466, 915, 959, 1554, 1676
XcmI CCANNNNNNNNNTGG 1 cut(s) 1490
XmiI GTMKAC 3 cut(s) 125, 804, 877
XmnI GAANNNNTTC 1 cut(s) 332
XspI CTAG 3 cut(s) 1368, 1646, 1802
ZraI GACGTC 1 cut(s) 205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.