Rw2G038460

Protein of unknown function (DUF3754)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0005827
Physical Location & Seq
Forward (+)
63403606 .. 63410408
6803 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G038460.1

Sequence Viewer

Length: 1848 bp
ATGTCAGCTTCTTACCACACTCTCACCAGCCTCACTTCTCTCTCCTTGACCTCAAAACGCCGTCGTACTATTATTCCTTCAACACCTCACTCGCTCACACTCGCTCGCTCTTCCCTCACCGGCTTCCGAGAAATCTCCACCGAGCCTAAGCAACCCCAAACCGACGCTGTAAACCTCACAGAAGCGCCGCTTCTTAAGAAGCAGAACGACGTCGTCCCGCAGAAGAAGAAGCGAGACGATGAAGACGAAGATGGAGATCAGGCGATTTCGAAGATTCCGGTTCCCAGGCAGAAGTACATCCCGGTCTCCAAGGCCGAGCTGCTAGACGGCGTCGTTTCGAATCTCTTCCGAGACCAGAACGGCGATGATTTGGAGCAGTTTCTTCTTCTTTCATCGTGCTTGGATTCGATTCTTCATGCCGAGCACAAAAACATTCTGGAGGAAATGCGAGCCGATTATTCGGAACCCAACTCCATGGAGTACAAGCAAGCAATTGAAGAGTTAGCAATGGCTAATGGGCAGGGCTCGGATTCTGCTGAAGGAATCAATGGAGTTGGGAGTGAAGAAAATGAAGCTGAAATTCCGTTCGATTATGTTGAGGGATTGAGGAAACTCTTGGGTTCTTCAGCTAAGGACGTCAAAAGAGTTGCCGTAGCCACTCGTTTCCAGCGCTCTTTCATGCAGCTTCTTTATGACGCTCAGTTTGAAGAACTATCAGCAAGGGATTTAATGTTGACATCTGCATTAAACTCAGATTATCTCCTTACTTTGCCAATATATGTTGACTGGAAGAAGGCTTCTCAGTCCAATGCAATAATATACAGGCGGGGATATGCAACTGAAAGGCAGAAGGGTCTACTACTTGTTGAAAAACTAGAGTACATACAGTCCAAACTTCTACGAGGAATCTTCTTCATTGTATCGAAACCATTGGGGGAAATTGGGAGCTGGATAACTGAGTCATTGAAAGTTGCTCTTCAGACACCAGAGGTACAAGAATGGACTGAAAAAATGAGACTTTGGCTAAAGGAATTCTCTGTTATTCAGCAAGCATTTCGTACTAACGAACAAACTTCTGATGATCTGCTGGGAGTAGATCAACTATCAGATAGGGAACTTCCAATATGGCTGGCAGCACAGAGAGCTGTATCTCGTTATGAAGGAATTTTATCCCCAGTTGGACCCCGTGGAAGGCTCTTAAGGAAGTTGCTTTCATGGATTGGACTTATTTCCCTTACACCAGAAACACCATTTGAGTTTGATGCTGATGCTAATGCTTCTGATCCTTATGCAAGGCCCATCTTCTTATCAAGGATATCACTTAGTGACATATGGAGACCTGCTACATGGAAATACTGTGAAAAGAATATATGGAAAATGTTGAAGACTTCTGTTTCCATTCTTTTCTCAAAGTCAATCCTCCAGGAGGCAGCATATCAAGAATTGATTTTGCTGTATACCAAAGAAGGAGATGACAAGAGTACTGAAGATAATGCTTCAGTTCCATCGTTGCAGTTAAAGATTTATGAGAAAATCCCTATTCCGGATCTACCAGTCATCTTTCCCCACAAAAAGCTCTCTTTCCGTATCATAGACACGGTGCGCTTGGACATTGCCTCAACATTGGCGCTTTCAGCATACTTCATAAACTACAAATTTGTTAATGTCTTATCTTCGCCGTCAGCAATATATCTTGATGTGGTTGCTATCAGTGCTCTTGTAATATATATCTCCCGTGTGGTTCTGGGATACAAGCAGACCTGGGATAGGTATCAAGTGAGTCAACTTCATTTTTCTCTGCAAGAGTTTATAACTATATGCTTTTCAAAATTGGAGGTGTTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3754

Protein Analysis

615

Amino Acids

69.84

Weight (kDa)

5.7

Isoelectric Point (pI)

44.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3754 PF12576 479 - 593 1.5e-25 Protein of unknown function (DUF3754)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1811
AatII GACGTC 2 cut(s) 213, 639
Acc36I ACCTGC 1 cut(s) 1348
AccI GTMKAC 2 cut(s) 856, 1457
AccIII TCCGGA 1 cut(s) 1543
AciI CCGC 3 cut(s) 188, 218, 826
AclWI GGATC 2 cut(s) 1277, 1554
AcsI RAATTY 4 cut(s) 579, 1031, 1164, 1655
AcuI CTGAAG 5 cut(s) 558, 609, 962, 1482, 1506
AcyI GRCGYC 3 cut(s) 210, 330, 636
AdeI CACNNNGTG 1 cut(s) 1325
AfaI GTAC 7 cut(s) 67, 296, 482, 881, 993, 1060, 1483
AfeI AGCGCT 1 cut(s) 671
AfiI CCNNNNNNNGG 4 cut(s) 1191, 1426, 1543, 1767
AflII CTTAAG 2 cut(s) 194, 1198
AgsI TTSAA 8 cut(s) 81, 497, 707, 869, 967, 1384, 1827, 1843
AjnI CCWGG 3 cut(s) 284, 1422, 1760
AluBI AGCT 8 cut(s) 8, 319, 575, 629, 685, 948, 1145, 1576
AluI AGCT 8 cut(s) 8, 319, 575, 629, 685, 948, 1145, 1576
Alw21I GWGCWC 2 cut(s) 426, 1717
Alw26I GTCTC 5 cut(s) 228, 310, 345, 1009, 1330
AlwI GGATC 2 cut(s) 1277, 1554
Aor13HI TCCGGA 1 cut(s) 1543
Aor51HI AGCGCT 1 cut(s) 671
AoxI GGCC 2 cut(s) 312, 1295
ApeKI GCWGC 4 cut(s) 319, 682, 1133, 1430
ApoI RAATTY 4 cut(s) 579, 1031, 1164, 1655
ArsI GACNNNNNNTTYG 2 cut(s) 686, 718
Asp700I GAANNNNTTC 1 cut(s) 344
AspLEI GCGC 4 cut(s) 187, 672, 1605, 1630
AspS9I GGNCC 2 cut(s) 1181, 1296
AsuC2I CCSGG 1 cut(s) 302
AsuHPI GGTGA 2 cut(s) 16, 109
AsuII TTCGAA 2 cut(s) 269, 338
AvaII GGWCC 1 cut(s) 1181
BanII GRGCYC 1 cut(s) 527
BbsI GAAGAC 2 cut(s) 249, 1391
Bbv12I GWGCWC 2 cut(s) 426, 1717
BbvI GCAGC 4 cut(s) 306, 694, 1145, 1442
BccI CCATC 3 cut(s) 245, 1307, 1513
BceAI ACGGC 5 cut(s) 45, 343, 376, 635, 1663
BcgI CGANNNNNNTGC 2 cut(s) 1037, 1071
BciT130I CCWGG 3 cut(s) 286, 1424, 1762
BciVI GTATCC 1 cut(s) 1742
BcnI CCSGG 1 cut(s) 302
BcoDI GTCTC 5 cut(s) 228, 310, 345, 1009, 1330
BfaI CTAG 2 cut(s) 323, 875
BfoI RGCGCY 3 cut(s) 188, 673, 1631
BfrI CTTAAG 2 cut(s) 194, 1198
BfuAI ACCTGC 1 cut(s) 1348
BfuI GTATCC 1 cut(s) 1742
BisI GCNGC 5 cut(s) 188, 320, 683, 1134, 1431
BlsI GCNGC 5 cut(s) 189, 321, 684, 1135, 1432
BmcAI AGTACT 1 cut(s) 1483
Bme1390I CCNGG 4 cut(s) 286, 302, 1424, 1762
Bme18I GGWCC 1 cut(s) 1181
BmgT120I GGNCC 2 cut(s) 1181, 1296
BmiI GGNNCC 3 cut(s) 282, 465, 1183
BmrFI CCNGG 4 cut(s) 286, 302, 1424, 1762
BmrI ACTGGG 1 cut(s) 1169
BmsI GCATC 2 cut(s) 1252, 1258
BmuI ACTGGG 1 cut(s) 1169
BpiI GAAGAC 2 cut(s) 249, 1391
BpmI CTGGAG 2 cut(s) 458, 1406
Bpu10I CCTNAGC 2 cut(s) 147, 630
Bpu14I TTCGAA 2 cut(s) 269, 338
BpuMI CCSGG 1 cut(s) 302
BsaBI GATNNNNATC 2 cut(s) 255, 1770
BsaHI GRCGYC 3 cut(s) 210, 330, 636
BsaI GGTCTC 3 cut(s) 310, 345, 1330
BsaJI CCNNGG 5 cut(s) 284, 309, 474, 1186, 1761
BsaWI WCCGGW 2 cut(s) 277, 1543
Bsc4I CCNNNNNNNGG 4 cut(s) 1191, 1426, 1543, 1767
Bse118I RCCGGY 1 cut(s) 119
Bse1I ACTGG 3 cut(s) 791, 1175, 1553
Bse3DI GCAATG 2 cut(s) 513, 1611
Bse8I GATNNNNATC 2 cut(s) 255, 1770
BseAI TCCGGA 1 cut(s) 1543
BseBI CCWGG 3 cut(s) 286, 1424, 1762
BseDI CCNNGG 5 cut(s) 284, 309, 474, 1186, 1761
BseGI GGATG 1 cut(s) 297
BseJI GATNNNNATC 2 cut(s) 255, 1770
BseLI CCNNNNNNNGG 4 cut(s) 1191, 1426, 1543, 1767
BseMI GCAATG 2 cut(s) 513, 1611
BseMII CTCAG 4 cut(s) 713, 765, 815, 948
BseNI ACTGG 3 cut(s) 791, 1175, 1553
BseXI GCAGC 4 cut(s) 306, 694, 1145, 1442
BseYI CCCAGC 1 cut(s) 1087
BshFI GGCC 2 cut(s) 314, 1297
BsiHKAI GWGCWC 2 cut(s) 426, 1717
BsiSI CCGG 4 cut(s) 120, 278, 302, 1544
BslFI GGGAC 1 cut(s) 200
BslI CCNNNNNNNGG 4 cut(s) 1191, 1426, 1543, 1767
BsmAI GTCTC 5 cut(s) 228, 310, 345, 1009, 1330
BsmBI CGTCTC 1 cut(s) 228
BsmFI GGGAC 1 cut(s) 200
BsnI GGCC 2 cut(s) 314, 1297
Bso31I GGTCTC 3 cut(s) 310, 345, 1330
Bsp119I TTCGAA 2 cut(s) 269, 338
Bsp1286I GDGCHC 3 cut(s) 426, 527, 1717
Bsp13I TCCGGA 1 cut(s) 1543
Bsp143I GATC 5 cut(s) 256, 1081, 1096, 1282, 1546
Bsp19I CCATGG 1 cut(s) 474
BspACI CCGC 3 cut(s) 188, 218, 826
BspANI GGCC 2 cut(s) 314, 1297
BspCNI CTCAG 4 cut(s) 712, 764, 814, 949
BspEI TCCGGA 1 cut(s) 1543
BspLI GGNNCC 3 cut(s) 282, 465, 1183
BspMI ACCTGC 1 cut(s) 1348
BspPI GGATC 2 cut(s) 1277, 1554
BspQI GCTCTTC 2 cut(s) 115, 981
BspT104I TTCGAA 2 cut(s) 269, 338
BspTI CTTAAG 2 cut(s) 194, 1198
BspTNI GGTCTC 3 cut(s) 310, 345, 1330
BsrDI GCAATG 2 cut(s) 513, 1611
BsrFI RCCGGY 1 cut(s) 119
BsrI ACTGG 3 cut(s) 791, 1175, 1553
BssAI RCCGGY 1 cut(s) 119
BssECI CCNNGG 5 cut(s) 284, 309, 474, 1186, 1761
BssMI GATC 5 cut(s) 256, 1081, 1096, 1282, 1546
BssNAI GTATAC 1 cut(s) 1458
BssNI GRCGYC 3 cut(s) 210, 330, 636
BssT1I CCWWGG 2 cut(s) 309, 474
Bst1107I GTATAC 1 cut(s) 1458
Bst2UI CCWGG 3 cut(s) 286, 1424, 1762
Bst4CI ACNGT 3 cut(s) 888, 1358, 1600
Bst6I CTCTTC 4 cut(s) 115, 350, 492, 981
BstACI GRCGYC 3 cut(s) 210, 330, 636
BstAFI CTTAAG 2 cut(s) 194, 1198
BstBI TTCGAA 2 cut(s) 269, 338
BstC8I GCNNGC 5 cut(s) 106, 450, 489, 1050, 1131
BstDEI CTNAG 7 cut(s) 147, 630, 699, 751, 801, 957, 1322
BstDSI CCRYGG 2 cut(s) 474, 1186
BstENI CCTNNNNNAGG 2 cut(s) 1424, 1765
BstF5I GGATG 1 cut(s) 297
BstH2I RGCGCY 3 cut(s) 188, 673, 1631
BstHHI GCGC 4 cut(s) 187, 672, 1605, 1630
BstKTI GATC 5 cut(s) 259, 1084, 1099, 1285, 1549
BstMAI GTCTC 5 cut(s) 228, 310, 345, 1009, 1330
BstMBI GATC 5 cut(s) 256, 1081, 1096, 1282, 1546
BstMWI GCNNNNNNNGC 3 cut(s) 1142, 1634, 1712
BstNI CCWGG 3 cut(s) 286, 1424, 1762
BstSCI CCNGG 4 cut(s) 284, 300, 1422, 1760
BstV1I GCAGC 4 cut(s) 306, 694, 1145, 1442
BstV2I GAAGAC 2 cut(s) 249, 1391
BstX2I RGATCY 1 cut(s) 1546
BstXI CCANNNNNNTGG 1 cut(s) 475
BstYI RGATCY 1 cut(s) 1546
BstZ17I GTATAC 1 cut(s) 1458
BsuI GTATCC 1 cut(s) 1742
BsuRI GGCC 2 cut(s) 314, 1297
BtgI CCRYGG 2 cut(s) 474, 1186
BtgZI GCGATG 1 cut(s) 378
BtsCI GGATG 1 cut(s) 297
BtsIMutI CAGTG 1 cut(s) 1717
BveI ACCTGC 1 cut(s) 1348
Cac8I GCNNGC 5 cut(s) 106, 450, 489, 1050, 1131
CfoI GCGC 4 cut(s) 187, 672, 1605, 1630
Cfr10I RCCGGY 1 cut(s) 119
Cfr13I GGNCC 2 cut(s) 1181, 1296
CseI GACGC 3 cut(s) 173, 319, 704
Csp6I GTAC 7 cut(s) 66, 295, 481, 880, 992, 1059, 1482
CviAII CATG 5 cut(s) 416, 475, 679, 1215, 1347
CviQI GTAC 7 cut(s) 66, 295, 481, 880, 992, 1059, 1482
DdeI CTNAG 7 cut(s) 147, 630, 699, 751, 801, 957, 1322
DpnI GATC 5 cut(s) 258, 1083, 1098, 1284, 1548
DpnII GATC 5 cut(s) 256, 1081, 1096, 1282, 1546
DraIII CACNNNGTG 1 cut(s) 1325
Eam1104I CTCTTC 4 cut(s) 115, 350, 492, 981
EarI CTCTTC 4 cut(s) 115, 350, 492, 981
Eco130I CCWWGG 2 cut(s) 309, 474
Eco24I GRGCYC 1 cut(s) 527
Eco31I GGTCTC 3 cut(s) 310, 345, 1330
Eco32I GATATC 1 cut(s) 1317
Eco47I GGWCC 1 cut(s) 1181
Eco47III AGCGCT 1 cut(s) 671
Eco57I CTGAAG 5 cut(s) 558, 609, 962, 1482, 1506
EcoNI CCTNNNNNAGG 2 cut(s) 1424, 1765
EcoRI GAATTC 1 cut(s) 1031
EcoRII CCWGG 3 cut(s) 284, 1422, 1760
EcoRV GATATC 1 cut(s) 1317
EcoT14I CCWWGG 2 cut(s) 309, 474
EcoT38I GRGCYC 1 cut(s) 527
ErhI CCWWGG 2 cut(s) 309, 474
Esp3I CGTCTC 1 cut(s) 228
FaeI CATG 5 cut(s) 419, 478, 682, 1218, 1350
FalI AAGNNNNNCTT 4 cut(s) 174, 206, 960, 992
FaqI GGGAC 1 cut(s) 200
FatI CATG 5 cut(s) 415, 474, 678, 1214, 1346
FauI CCCGC 2 cut(s) 225, 819
FauNDI CATATG 1 cut(s) 1331
FblI GTMKAC 2 cut(s) 856, 1457
Fnu4HI GCNGC 5 cut(s) 188, 320, 683, 1134, 1431
FokI GGATG 1 cut(s) 284
FriOI GRGCYC 1 cut(s) 527
Fsp4HI GCNGC 5 cut(s) 188, 320, 683, 1134, 1431
FspBI CTAG 2 cut(s) 323, 875
GlaI GCGC 4 cut(s) 186, 671, 1604, 1629
GluI GCNGC 5 cut(s) 188, 320, 683, 1134, 1431
GsaI CCCAGC 1 cut(s) 1091
GsuI CTGGAG 2 cut(s) 458, 1406
HaeII RGCGCY 3 cut(s) 188, 673, 1631
HaeIII GGCC 2 cut(s) 314, 1297
HapII CCGG 4 cut(s) 120, 278, 302, 1544
HgaI GACGC 3 cut(s) 173, 319, 704
HhaI GCGC 4 cut(s) 187, 672, 1605, 1630
Hin1I GRCGYC 3 cut(s) 210, 330, 636
Hin1II CATG 5 cut(s) 419, 478, 682, 1218, 1350
Hin6I GCGC 4 cut(s) 185, 670, 1603, 1628
HinP1I GCGC 4 cut(s) 185, 670, 1603, 1628
HincII GTYRAC 3 cut(s) 735, 784, 1784
HindII GTYRAC 3 cut(s) 735, 784, 1784
HinfI GANTC 9 cut(s) 274, 340, 404, 409, 530, 543, 906, 959, 1780
HpaII CCGG 4 cut(s) 120, 278, 302, 1544
HphI GGTGA 2 cut(s) 16, 109
Hpy166II GTNNAC 6 cut(s) 172, 735, 784, 857, 1458, 1784
Hpy188I TCNGA 9 cut(s) 128, 350, 463, 529, 754, 981, 1078, 1108, 1282
Hpy188III TCNNGA 4 cut(s) 437, 1439, 1544, 1694
Hpy8I GTNNAC 6 cut(s) 172, 735, 784, 857, 1458, 1784
Hpy99I CGWCG 5 cut(s) 66, 167, 212, 215, 335
HpyAV CCTTC 7 cut(s) 87, 533, 787, 844, 1154, 1185, 1460
HpyCH4III ACNGT 3 cut(s) 888, 1358, 1600
HpyCH4IV ACGT 2 cut(s) 210, 636
HpyCH4V TGCA 7 cut(s) 682, 743, 812, 836, 1292, 1513, 1801
HpyF10VI GCNNNNNNNGC 3 cut(s) 1142, 1634, 1712
HpyF3I CTNAG 7 cut(s) 147, 630, 699, 751, 801, 957, 1322
HpySE526I ACGT 2 cut(s) 210, 636
Hsp92I GRCGYC 3 cut(s) 210, 330, 636
Hsp92II CATG 5 cut(s) 419, 478, 682, 1218, 1350
HspAI GCGC 4 cut(s) 185, 670, 1603, 1628
Kpn2I TCCGGA 1 cut(s) 1543
Kzo9I GATC 5 cut(s) 256, 1081, 1096, 1282, 1546
LguI GCTCTTC 2 cut(s) 115, 981
LmnI GCTCC 2 cut(s) 373, 945
Lsp1109I GCAGC 4 cut(s) 306, 694, 1145, 1442
LweI GCATC 2 cut(s) 1252, 1258
MaeI CTAG 2 cut(s) 323, 875
MaeII ACGT 2 cut(s) 210, 636
MaeIII GTNAC 1 cut(s) 1325
MalI GATC 5 cut(s) 258, 1083, 1098, 1284, 1548
MboI GATC 5 cut(s) 256, 1081, 1096, 1282, 1546
MfeI CAATTG 1 cut(s) 492
MflI RGATCY 1 cut(s) 1546
MhlI GDGCHC 3 cut(s) 426, 527, 1717
MluCI AATT 8 cut(s) 492, 579, 939, 1031, 1164, 1442, 1655, 1829
MlyI GAGTC 2 cut(s) 968, 1789
MmeI TCCRAC 1 cut(s) 1159
MroI TCCGGA 1 cut(s) 1543
MroXI GAANNNNTTC 1 cut(s) 344
MseI TTAA 6 cut(s) 195, 728, 746, 1199, 1517, 1662
MspCI CTTAAG 2 cut(s) 194, 1198
MspI CCGG 4 cut(s) 120, 278, 302, 1544
MspR9I CCNGG 4 cut(s) 286, 302, 1424, 1762
MunI CAATTG 1 cut(s) 492
MvaI CCWGG 3 cut(s) 286, 1424, 1762
MwoI GCNNNNNNNGC 3 cut(s) 1142, 1634, 1712
NciI CCSGG 1 cut(s) 302
NcoI CCATGG 1 cut(s) 474
NdeI CATATG 1 cut(s) 1331
NdeII GATC 5 cut(s) 256, 1081, 1096, 1282, 1546
NlaIII CATG 5 cut(s) 419, 478, 682, 1218, 1350
NlaIV GGNNCC 3 cut(s) 282, 465, 1183
NmeAIII GCCGAG 2 cut(s) 340, 445
NmuCI GTSAC 1 cut(s) 1325
NspV TTCGAA 2 cut(s) 269, 338
PciSI GCTCTTC 2 cut(s) 115, 981
PcsI WCGNNNNNNNCGW 1 cut(s) 243
PdmI GAANNNNTTC 1 cut(s) 344
PfeI GAWTC 7 cut(s) 274, 340, 404, 409, 530, 543, 906
PflFI GACNNNGTC 2 cut(s) 212, 329
PfoI TCCNGGA 1 cut(s) 1422
PkrI GCNGC 5 cut(s) 189, 321, 684, 1135, 1432
PleI GAGTC 2 cut(s) 967, 1788
PpsI GAGTC 2 cut(s) 967, 1788
PsiI TTATAA 1 cut(s) 1811
Psp6I CCWGG 3 cut(s) 284, 1422, 1760
PspFI CCCAGC 1 cut(s) 1087
PspGI CCWGG 3 cut(s) 284, 1422, 1760
PspN4I GGNNCC 3 cut(s) 282, 465, 1183
PspPI GGNCC 2 cut(s) 1181, 1296
PsuI RGATCY 1 cut(s) 1546
PsyI GACNNNGTC 2 cut(s) 212, 329
RsaI GTAC 7 cut(s) 67, 296, 482, 881, 993, 1060, 1483
RsaNI GTAC 7 cut(s) 66, 295, 481, 880, 992, 1059, 1482
SapI GCTCTTC 2 cut(s) 115, 981
SaqAI TTAA 6 cut(s) 195, 728, 746, 1199, 1517, 1662
SatI GCNGC 5 cut(s) 188, 320, 683, 1134, 1431
Sau3AI GATC 5 cut(s) 256, 1081, 1096, 1282, 1546
Sau96I GGNCC 2 cut(s) 1181, 1296
ScaI AGTACT 1 cut(s) 1483
SchI GAGTC 2 cut(s) 968, 1789
ScrFI CCNGG 4 cut(s) 286, 302, 1424, 1762
SduI GDGCHC 3 cut(s) 426, 527, 1717
SfaNI GCATC 2 cut(s) 1252, 1258
SfuI TTCGAA 2 cut(s) 269, 338
SinI GGWCC 1 cut(s) 1181
SmlI CTYRAG 2 cut(s) 194, 1198
SmoI CTYRAG 2 cut(s) 194, 1198
Sse9I AATT 8 cut(s) 492, 579, 939, 1031, 1164, 1442, 1655, 1829
SsiI CCGC 3 cut(s) 188, 218, 826
SspMI CTAG 2 cut(s) 323, 875
StyD4I CCNGG 4 cut(s) 284, 300, 1422, 1760
StyI CCWWGG 2 cut(s) 309, 474
TaaI ACNGT 3 cut(s) 888, 1358, 1600
TaiI ACGT 2 cut(s) 213, 639
TaqI TCGA 5 cut(s) 269, 338, 407, 588, 923
TasI AATT 8 cut(s) 492, 579, 939, 1031, 1164, 1442, 1655, 1829
TatI WGTACW 4 cut(s) 294, 480, 879, 1481
TauI GCSGC 1 cut(s) 190
TfiI GAWTC 7 cut(s) 274, 340, 404, 409, 530, 543, 906
Tru1I TTAA 6 cut(s) 195, 728, 746, 1199, 1517, 1662
Tru9I TTAA 6 cut(s) 195, 728, 746, 1199, 1517, 1662
TscAI CASTG 1 cut(s) 1717
TseFI GTSAC 1 cut(s) 1325
TseI GCWGC 4 cut(s) 319, 682, 1133, 1430
Tsp45I GTSAC 1 cut(s) 1325
TspGWI ACGGA 2 cut(s) 573, 1574
TspRI CASTG 1 cut(s) 1717
Tth111I GACNNNGTC 2 cut(s) 212, 329
Vha464I CTTAAG 2 cut(s) 194, 1198
VpaK11BI GGWCC 1 cut(s) 1181
XagI CCTNNNNNAGG 2 cut(s) 1424, 1765
XapI RAATTY 4 cut(s) 579, 1031, 1164, 1655
XmiI GTMKAC 2 cut(s) 856, 1457
XmnI GAANNNNTTC 1 cut(s) 344
XspI CTAG 2 cut(s) 323, 875
ZraI GACGTC 2 cut(s) 211, 637
ZrmI AGTACT 1 cut(s) 1483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.