RchiOBHm_Chr2g0149491

Protein of unknown function (DUF3754)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0005827
Physical Location & Seq
Forward (+)
67324578 .. 67331351
6774 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51889

Sequence Viewer

Length: 2202 bp
ATGTCAGCTTCTTACCACACTCTCACCAGCCTCACTTCTCTCTCCTTGACCTCAAAACGCCGTCGTACTATTATTCCTTCAACACCTCACTCGCTCACACTCGCTCGCTCTTCCCTCACCGGCTTCCGAGAAATCTCCACCGAGCCGAAGCAACCCCAAACCGACGCTGTAAACCTCACAGAAGCGCCGCTTCTTAAGAAGCAGAACGACGTCGTCCCGCAGAAGAAGAAGCGAGACGATGAAGACGAAGATGGAGATCAGGCGATTTCGAAGATTCCGGTTCCCAGGCAGAAGTACATCCCGGTCTCCAAGGCCGAGCTGCTAGACGGCGTCGTTTCGAATCTCTTCCGAGACCAGAACGGCGATGATTTGGAGCAGTTTCTTCTTCTTTCATCGTGCTTGGATTCGATTCTTCATGCCGAGCACAAAAACATTCTGGAGGAAATGCGAGCCGATTATTCGGAACCCAACTCCATGGAGTACAAGCAAGCAATTGAAGAGTTAGCAATGGCTAATGGGCAGGGCTCGGATTCTGCTGAAGGAATCAATGGAGTTGGGAGTGAAGAAAATGAAGCTGAAATTCCGTTCGATTATGTTGAGGGACTGAGGAAACTCTTGGGTTCTTCAGCTAAGGACGTCAAAAGAGTTGCCGTAGCCACTCGTTTCCAGCGCTCTTTCATGCAGCTTCTTTATGACGCTCAGTTTGAAGAACTATCAGCAAGGGATTTAATGTTGACATCTGCATTAAACTCAGATTATCTCCTTACTTTGCCAATATATGTTGACTGGAAGAAGGCTTCTCAGTCCAATGCAATAATATACAGGCGGGGATATGCAACTGAAAGGCAGAAGGGTCTACTACTTGTTGAAAAACTAGAGTACATACAATCCAAACTTCTACGAGGAATCTTCTTCATTGTATCGAAACCATTGGGGGAAATTGGGAGCTGGATAACTGAGTCATTGAAAGTTGCTCTTCAGACACCAGAGGTACAAGAATGGACTGAAAAAATGAGACTTTGGCTAAAGGAATTCTCTGTTATTCAGCAAGCATTTCGTACTAACGAACAAACTTCTGATGATCTGTTGGGAGTAGATCAACTATCAGATAGGGAACTTCCAATATGGCTGGCAGCACAGAGAGCTGTATCTCGTTATGAAGGAATTTTATCCCCAGTTGGACCCCGTGGAAGGCTCTTAAGGAAGTTGCTTTCATGGATTGGACTTATTTCCCTTACACCAGAAACACCATTTGAGTTTGATGCTGATGCTAATGCTTCTGATCCTTATGCAAGGCCCATCTTCTTATCAAGGATATCACTTAGTGACATATGGAGACCTGCTACATGGAAATACTGTGAAAAGAATATATGGAAAATGTTGAAGACTTCTGTTTCCATTCTTTTATCAAAGTCAATCCTCCAGGAGGCAGCATATCAAGAATTGATTTTGCTGTATACCAAAGAAGGAGATGACAAGAGTACTGAAGATAATGCTTCAGTTCCATCGTTGCAGTTAAAGATTTATGAGAAAATCCCTATTCCGGATCTACCAGTAAATAATTCTGTCTCAATTTTAACCCTCATCCAATGTAATTGTCATTGCTTTAGTGTCATCTTTCCCCACAAAAAGCTCTCTTTCCGTATCATAGACACGGTGCGCTTGGACATTGCCTCAACATTGGCGCTTTCAGCATACTTCATAAACTACAAATTTGTTAATGTCTTATCTTCGCCGTCAGCAATATATCTTGATGTGGTCGCTATCAGTGCTCTTGTAATATATATCTCCCGTGTGGTTCTGGGATACAAACAGACCTGGGATAGGTATCAACTTTTGGTCAACAAGACGCTTTATGAGAAAACCTTAGCTAGTGGATTTGGTTCAGTTCATTTTTTGCTGGATGCTTCTGAACAGCAACAATACAAGGAAGCCATTTTGGCGTATGCAATCCTTCTCAAAGCTAAGGAGGGAAAGCTTACAAGTCGCGGGAGTGTTGGAGACACATGCGAAAGATTTATGTATGATGTGTTTAAAGTAAAGGTTGAAATGCCAGTTGACAAGGCAATTGCTACATTATTAAGACTGGGGCTTGCTGCAGAAACAAGTATTGGTGGAAGGATTACTCTGCAAGCTATTCCATGTTCCAGTGCATATGATTCCCTTAAACAACGTTGGAATAGTTTACTCAGTCAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3754

Protein Analysis

733

Amino Acids

82.59

Weight (kDa)

6.02

Isoelectric Point (pI)

44.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3754 PF12576 537 - 628 2.6e-21 Protein of unknown function (DUF3754)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 213, 639
Acc36I ACCTGC 1 cut(s) 1348
AccI GTMKAC 2 cut(s) 856, 1457
AccII CGCG 1 cut(s) 1989
AccIII TCCGGA 1 cut(s) 1543
AciI CCGC 4 cut(s) 188, 218, 826, 1989
AclI AACGTT 1 cut(s) 2173
AclWI GGATC 2 cut(s) 1277, 1554
AcsI RAATTY 4 cut(s) 579, 1031, 1164, 1712
AcuI CTGAAG 5 cut(s) 558, 609, 962, 1482, 1506
AcyI GRCGYC 3 cut(s) 210, 330, 636
AdeI CACNNNGTG 1 cut(s) 1325
AfaI GTAC 7 cut(s) 67, 296, 482, 881, 993, 1060, 1483
AfeI AGCGCT 1 cut(s) 671
AfiI CCNNNNNNNGG 4 cut(s) 1191, 1426, 1543, 1824
AflII CTTAAG 2 cut(s) 194, 1198
AgsI TTSAA 7 cut(s) 81, 497, 707, 869, 967, 1384, 2048
AjnI CCWGG 3 cut(s) 284, 1422, 1817
AjuI GAANNNNNNNTTGG 2 cut(s) 2094, 2126
Alw21I GWGCWC 2 cut(s) 426, 1774
Alw26I GTCTC 7 cut(s) 228, 310, 345, 1009, 1330, 1573, 1995
AlwI GGATC 2 cut(s) 1277, 1554
Aor13HI TCCGGA 1 cut(s) 1543
Aor51HI AGCGCT 1 cut(s) 671
AoxI GGCC 2 cut(s) 312, 1295
ApeKI GCWGC 5 cut(s) 319, 682, 1133, 1430, 2096
ApoI RAATTY 4 cut(s) 579, 1031, 1164, 1712
ArsI GACNNNNNNTTYG 2 cut(s) 686, 718
Asp700I GAANNNNTTC 1 cut(s) 344
AspLEI GCGC 4 cut(s) 187, 672, 1662, 1687
AspS9I GGNCC 2 cut(s) 1181, 1296
AsuC2I CCSGG 1 cut(s) 302
AsuHPI GGTGA 2 cut(s) 16, 109
AsuII TTCGAA 2 cut(s) 269, 338
AvaII GGWCC 1 cut(s) 1181
BanII GRGCYC 1 cut(s) 527
BbsI GAAGAC 2 cut(s) 249, 1391
Bbv12I GWGCWC 2 cut(s) 426, 1774
BbvI GCAGC 5 cut(s) 306, 694, 1145, 1442, 2083
BccI CCATC 3 cut(s) 245, 1307, 1513
BceAI ACGGC 5 cut(s) 45, 343, 376, 635, 1720
BcgI CGANNNNNNTGC 2 cut(s) 1037, 1071
BciT130I CCWGG 3 cut(s) 286, 1424, 1819
BciVI GTATCC 1 cut(s) 1799
BcnI CCSGG 1 cut(s) 302
BcoDI GTCTC 7 cut(s) 228, 310, 345, 1009, 1330, 1573, 1995
BfaI CTAG 3 cut(s) 323, 875, 1872
BfmI CTRYAG 1 cut(s) 2097
BfoI RGCGCY 3 cut(s) 188, 673, 1688
BfrI CTTAAG 2 cut(s) 194, 1198
BfuAI ACCTGC 1 cut(s) 1348
BfuI GTATCC 1 cut(s) 1799
BglI GCCNNNNNGGC 1 cut(s) 1940
BisI GCNGC 6 cut(s) 188, 320, 683, 1134, 1431, 2097
BlsI GCNGC 6 cut(s) 189, 321, 684, 1135, 1432, 2098
BmcAI AGTACT 1 cut(s) 1483
Bme1390I CCNGG 4 cut(s) 286, 302, 1424, 1819
Bme18I GGWCC 1 cut(s) 1181
BmgT120I GGNCC 2 cut(s) 1181, 1296
BmiI GGNNCC 3 cut(s) 282, 465, 1183
BmrFI CCNGG 4 cut(s) 286, 302, 1424, 1819
BmrI ACTGGG 2 cut(s) 1169, 2096
BmsI GCATC 3 cut(s) 1252, 1258, 1894
BmuI ACTGGG 2 cut(s) 1169, 2096
BpiI GAAGAC 2 cut(s) 249, 1391
BpmI CTGGAG 2 cut(s) 458, 1406
Bpu10I CCTNAGC 3 cut(s) 630, 1867, 1965
Bpu14I TTCGAA 2 cut(s) 269, 338
BpuMI CCSGG 1 cut(s) 302
BsaBI GATNNNNATC 2 cut(s) 255, 1827
BsaHI GRCGYC 3 cut(s) 210, 330, 636
BsaI GGTCTC 3 cut(s) 310, 345, 1330
BsaJI CCNNGG 5 cut(s) 284, 309, 474, 1186, 1818
BsaWI WCCGGW 2 cut(s) 277, 1543
Bsc4I CCNNNNNNNGG 4 cut(s) 1191, 1426, 1543, 1824
Bse118I RCCGGY 1 cut(s) 119
Bse1I ACTGG 6 cut(s) 791, 1175, 1553, 2054, 2091, 2148
Bse3DI GCAATG 3 cut(s) 513, 1600, 1668
Bse8I GATNNNNATC 2 cut(s) 255, 1827
BseAI TCCGGA 1 cut(s) 1543
BseBI CCWGG 3 cut(s) 286, 1424, 1819
BseDI CCNNGG 5 cut(s) 284, 309, 474, 1186, 1818
BseGI GGATG 3 cut(s) 297, 1584, 1909
BseJI GATNNNNATC 2 cut(s) 255, 1827
BseLI CCNNNNNNNGG 4 cut(s) 1191, 1426, 1543, 1824
BseMI GCAATG 3 cut(s) 513, 1600, 1668
BseMII CTCAG 5 cut(s) 596, 713, 765, 815, 948
BseNI ACTGG 6 cut(s) 791, 1175, 1553, 2054, 2091, 2148
BseXI GCAGC 5 cut(s) 306, 694, 1145, 1442, 2083
Bsh1236I CGCG 1 cut(s) 1989
BshFI GGCC 2 cut(s) 314, 1297
BsiHKAI GWGCWC 2 cut(s) 426, 1774
BsiSI CCGG 4 cut(s) 120, 278, 302, 1544
BslFI GGGAC 2 cut(s) 200, 615
BslI CCNNNNNNNGG 4 cut(s) 1191, 1426, 1543, 1824
BsmAI GTCTC 7 cut(s) 228, 310, 345, 1009, 1330, 1573, 1995
BsmBI CGTCTC 1 cut(s) 228
BsmFI GGGAC 2 cut(s) 200, 615
BsnI GGCC 2 cut(s) 314, 1297
Bso31I GGTCTC 3 cut(s) 310, 345, 1330
Bsp119I TTCGAA 2 cut(s) 269, 338
Bsp1286I GDGCHC 3 cut(s) 426, 527, 1774
Bsp13I TCCGGA 1 cut(s) 1543
Bsp143I GATC 5 cut(s) 256, 1081, 1096, 1282, 1546
Bsp19I CCATGG 1 cut(s) 474
BspACI CCGC 4 cut(s) 188, 218, 826, 1989
BspANI GGCC 2 cut(s) 314, 1297
BspCNI CTCAG 6 cut(s) 597, 712, 764, 814, 949, 2202
BspEI TCCGGA 1 cut(s) 1543
BspFNI CGCG 1 cut(s) 1989
BspLI GGNNCC 3 cut(s) 282, 465, 1183
BspMAI CTGCAG 1 cut(s) 2101
BspMI ACCTGC 1 cut(s) 1348
BspPI GGATC 2 cut(s) 1277, 1554
BspQI GCTCTTC 2 cut(s) 115, 981
BspT104I TTCGAA 2 cut(s) 269, 338
BspTI CTTAAG 2 cut(s) 194, 1198
BspTNI GGTCTC 3 cut(s) 310, 345, 1330
BsrDI GCAATG 3 cut(s) 513, 1600, 1668
BsrFI RCCGGY 1 cut(s) 119
BsrI ACTGG 6 cut(s) 791, 1175, 1553, 2054, 2091, 2148
BssAI RCCGGY 1 cut(s) 119
BssECI CCNNGG 5 cut(s) 284, 309, 474, 1186, 1818
BssMI GATC 5 cut(s) 256, 1081, 1096, 1282, 1546
BssNAI GTATAC 1 cut(s) 1458
BssNI GRCGYC 3 cut(s) 210, 330, 636
BssT1I CCWWGG 2 cut(s) 309, 474
Bst1107I GTATAC 1 cut(s) 1458
Bst2UI CCWGG 3 cut(s) 286, 1424, 1819
Bst4CI ACNGT 2 cut(s) 1358, 1657
Bst6I CTCTTC 4 cut(s) 115, 350, 492, 981
BstACI GRCGYC 3 cut(s) 210, 330, 636
BstAFI CTTAAG 2 cut(s) 194, 1198
BstBI TTCGAA 2 cut(s) 269, 338
BstC8I GCNNGC 7 cut(s) 106, 450, 489, 1050, 1131, 2094, 2133
BstDSI CCRYGG 2 cut(s) 474, 1186
BstENI CCTNNNNNAGG 2 cut(s) 1424, 1822
BstF5I GGATG 3 cut(s) 297, 1584, 1909
BstFNI CGCG 1 cut(s) 1989
BstH2I RGCGCY 3 cut(s) 188, 673, 1688
BstHHI GCGC 4 cut(s) 187, 672, 1662, 1687
BstKTI GATC 5 cut(s) 259, 1084, 1099, 1285, 1549
BstMAI GTCTC 7 cut(s) 228, 310, 345, 1009, 1330, 1573, 1995
BstMBI GATC 5 cut(s) 256, 1081, 1096, 1282, 1546
BstMWI GCNNNNNNNGC 4 cut(s) 1142, 1691, 1769, 1940
BstNI CCWGG 3 cut(s) 286, 1424, 1819
BstNSI RCATGY 1 cut(s) 2010
BstSCI CCNGG 4 cut(s) 284, 300, 1422, 1817
BstSFI CTRYAG 1 cut(s) 2097
BstUI CGCG 1 cut(s) 1989
BstV1I GCAGC 5 cut(s) 306, 694, 1145, 1442, 2083
BstV2I GAAGAC 2 cut(s) 249, 1391
BstX2I RGATCY 1 cut(s) 1546
BstXI CCANNNNNNTGG 1 cut(s) 475
BstYI RGATCY 1 cut(s) 1546
BstZ17I GTATAC 1 cut(s) 1458
BsuI GTATCC 1 cut(s) 1799
BsuRI GGCC 2 cut(s) 314, 1297
BtgI CCRYGG 2 cut(s) 474, 1186
BtgZI GCGATG 1 cut(s) 378
BtsCI GGATG 3 cut(s) 297, 1584, 1909
BtsIMutI CAGTG 2 cut(s) 1774, 2155
BveI ACCTGC 1 cut(s) 1348
Cac8I GCNNGC 7 cut(s) 106, 450, 489, 1050, 1131, 2094, 2133
CfoI GCGC 4 cut(s) 187, 672, 1662, 1687
Cfr10I RCCGGY 1 cut(s) 119
Cfr13I GGNCC 2 cut(s) 1181, 1296
CseI GACGC 4 cut(s) 173, 319, 704, 1858
Csp6I GTAC 7 cut(s) 66, 295, 481, 880, 992, 1059, 1482
CviAII CATG 7 cut(s) 416, 475, 679, 1215, 1347, 2007, 2142
CviQI GTAC 7 cut(s) 66, 295, 481, 880, 992, 1059, 1482
DpnI GATC 5 cut(s) 258, 1083, 1098, 1284, 1548
DpnII GATC 5 cut(s) 256, 1081, 1096, 1282, 1546
DraI TTTAAA 1 cut(s) 2035
DraIII CACNNNGTG 1 cut(s) 1325
Eam1104I CTCTTC 4 cut(s) 115, 350, 492, 981
EarI CTCTTC 4 cut(s) 115, 350, 492, 981
Eco130I CCWWGG 2 cut(s) 309, 474
Eco24I GRGCYC 1 cut(s) 527
Eco31I GGTCTC 3 cut(s) 310, 345, 1330
Eco32I GATATC 1 cut(s) 1317
Eco47I GGWCC 1 cut(s) 1181
Eco47III AGCGCT 1 cut(s) 671
Eco57I CTGAAG 5 cut(s) 558, 609, 962, 1482, 1506
EcoNI CCTNNNNNAGG 2 cut(s) 1424, 1822
EcoRI GAATTC 1 cut(s) 1031
EcoRII CCWGG 3 cut(s) 284, 1422, 1817
EcoRV GATATC 1 cut(s) 1317
EcoT14I CCWWGG 2 cut(s) 309, 474
EcoT38I GRGCYC 1 cut(s) 527
ErhI CCWWGG 2 cut(s) 309, 474
Esp3I CGTCTC 1 cut(s) 228
FaeI CATG 7 cut(s) 419, 478, 682, 1218, 1350, 2010, 2145
FalI AAGNNNNNCTT 4 cut(s) 174, 206, 960, 992
FaqI GGGAC 2 cut(s) 200, 615
FatI CATG 7 cut(s) 415, 474, 678, 1214, 1346, 2006, 2141
FauI CCCGC 3 cut(s) 225, 819, 1982
FauNDI CATATG 2 cut(s) 1331, 2155
FblI GTMKAC 2 cut(s) 856, 1457
Fnu4HI GCNGC 6 cut(s) 188, 320, 683, 1134, 1431, 2097
FokI GGATG 3 cut(s) 284, 1571, 1916
FriOI GRGCYC 1 cut(s) 527
Fsp4HI GCNGC 6 cut(s) 188, 320, 683, 1134, 1431, 2097
FspBI CTAG 3 cut(s) 323, 875, 1872
GlaI GCGC 4 cut(s) 186, 671, 1661, 1686
GluI GCNGC 6 cut(s) 188, 320, 683, 1134, 1431, 2097
GsuI CTGGAG 2 cut(s) 458, 1406
HaeII RGCGCY 3 cut(s) 188, 673, 1688
HaeIII GGCC 2 cut(s) 314, 1297
HapII CCGG 4 cut(s) 120, 278, 302, 1544
HgaI GACGC 4 cut(s) 173, 319, 704, 1858
HhaI GCGC 4 cut(s) 187, 672, 1662, 1687
Hin1I GRCGYC 3 cut(s) 210, 330, 636
Hin1II CATG 7 cut(s) 419, 478, 682, 1218, 1350, 2010, 2145
Hin6I GCGC 4 cut(s) 185, 670, 1660, 1685
HinP1I GCGC 4 cut(s) 185, 670, 1660, 1685
HincII GTYRAC 4 cut(s) 735, 784, 1843, 2059
HindII GTYRAC 4 cut(s) 735, 784, 1843, 2059
HindIII AAGCTT 1 cut(s) 1976
HinfI GANTC 9 cut(s) 274, 340, 404, 409, 530, 543, 906, 959, 2159
HpaII CCGG 4 cut(s) 120, 278, 302, 1544
HphI GGTGA 2 cut(s) 16, 109
Hpy166II GTNNAC 8 cut(s) 172, 735, 784, 857, 1458, 1843, 2059, 2186
Hpy188III TCNNGA 5 cut(s) 437, 1439, 1544, 1751, 2195
Hpy8I GTNNAC 8 cut(s) 172, 735, 784, 857, 1458, 1843, 2059, 2186
Hpy99I CGWCG 5 cut(s) 66, 167, 212, 215, 335
HpyAV CCTTC 9 cut(s) 87, 533, 787, 844, 1154, 1185, 1460, 1964, 2112
HpyCH4III ACNGT 2 cut(s) 1358, 1657
HpyCH4IV ACGT 3 cut(s) 210, 636, 2173
HpyF10VI GCNNNNNNNGC 4 cut(s) 1142, 1691, 1769, 1940
HpySE526I ACGT 3 cut(s) 210, 636, 2173
Hsp92I GRCGYC 3 cut(s) 210, 330, 636
Hsp92II CATG 7 cut(s) 419, 478, 682, 1218, 1350, 2010, 2145
HspAI GCGC 4 cut(s) 185, 670, 1660, 1685
Kpn2I TCCGGA 1 cut(s) 1543
Kzo9I GATC 5 cut(s) 256, 1081, 1096, 1282, 1546
LguI GCTCTTC 2 cut(s) 115, 981
LmnI GCTCC 2 cut(s) 373, 945
Lsp1109I GCAGC 5 cut(s) 306, 694, 1145, 1442, 2083
LweI GCATC 3 cut(s) 1252, 1258, 1894
MaeI CTAG 3 cut(s) 323, 875, 1872
MaeII ACGT 3 cut(s) 210, 636, 2173
MaeIII GTNAC 1 cut(s) 1325
MalI GATC 5 cut(s) 258, 1083, 1098, 1284, 1548
MboI GATC 5 cut(s) 256, 1081, 1096, 1282, 1546
MfeI CAATTG 2 cut(s) 492, 2067
MflI RGATCY 1 cut(s) 1546
MhlI GDGCHC 3 cut(s) 426, 527, 1774
MlyI GAGTC 1 cut(s) 968
MmeI TCCRAC 3 cut(s) 1159, 1978, 2156
MroI TCCGGA 1 cut(s) 1543
MroXI GAANNNNTTC 1 cut(s) 344
MspCI CTTAAG 2 cut(s) 194, 1198
MspI CCGG 4 cut(s) 120, 278, 302, 1544
MspR9I CCNGG 4 cut(s) 286, 302, 1424, 1819
MunI CAATTG 2 cut(s) 492, 2067
MvaI CCWGG 3 cut(s) 286, 1424, 1819
MvnI CGCG 1 cut(s) 1989
MwoI GCNNNNNNNGC 4 cut(s) 1142, 1691, 1769, 1940
NciI CCSGG 1 cut(s) 302
NcoI CCATGG 1 cut(s) 474
NdeI CATATG 2 cut(s) 1331, 2155
NdeII GATC 5 cut(s) 256, 1081, 1096, 1282, 1546
NlaIII CATG 7 cut(s) 419, 478, 682, 1218, 1350, 2010, 2145
NlaIV GGNNCC 3 cut(s) 282, 465, 1183
NmeAIII GCCGAG 2 cut(s) 340, 445
NmuCI GTSAC 1 cut(s) 1325
NspI RCATGY 1 cut(s) 2010
NspV TTCGAA 2 cut(s) 269, 338
PciSI GCTCTTC 2 cut(s) 115, 981
PcsI WCGNNNNNNNCGW 1 cut(s) 243
PdmI GAANNNNTTC 1 cut(s) 344
PfeI GAWTC 8 cut(s) 274, 340, 404, 409, 530, 543, 906, 2159
PflFI GACNNNGTC 2 cut(s) 212, 329
PfoI TCCNGGA 1 cut(s) 1422
PkrI GCNGC 6 cut(s) 189, 321, 684, 1135, 1432, 2098
PleI GAGTC 1 cut(s) 967
PpsI GAGTC 1 cut(s) 967
Psp1406I AACGTT 1 cut(s) 2173
Psp6I CCWGG 3 cut(s) 284, 1422, 1817
PspGI CCWGG 3 cut(s) 284, 1422, 1817
PspN4I GGNNCC 3 cut(s) 282, 465, 1183
PspPI GGNCC 2 cut(s) 1181, 1296
PstI CTGCAG 1 cut(s) 2101
PsuI RGATCY 1 cut(s) 1546
PsyI GACNNNGTC 2 cut(s) 212, 329
RsaI GTAC 7 cut(s) 67, 296, 482, 881, 993, 1060, 1483
RsaNI GTAC 7 cut(s) 66, 295, 481, 880, 992, 1059, 1482
SapI GCTCTTC 2 cut(s) 115, 981
SatI GCNGC 6 cut(s) 188, 320, 683, 1134, 1431, 2097
Sau3AI GATC 5 cut(s) 256, 1081, 1096, 1282, 1546
Sau96I GGNCC 2 cut(s) 1181, 1296
ScaI AGTACT 1 cut(s) 1483
SchI GAGTC 1 cut(s) 968
ScrFI CCNGG 4 cut(s) 286, 302, 1424, 1819
SduI GDGCHC 3 cut(s) 426, 527, 1774
SfaNI GCATC 3 cut(s) 1252, 1258, 1894
SfcI CTRYAG 1 cut(s) 2097
SfuI TTCGAA 2 cut(s) 269, 338
SinI GGWCC 1 cut(s) 1181
SmlI CTYRAG 2 cut(s) 194, 1198
SmoI CTYRAG 2 cut(s) 194, 1198
SsiI CCGC 4 cut(s) 188, 218, 826, 1989
SspMI CTAG 3 cut(s) 323, 875, 1872
StyD4I CCNGG 4 cut(s) 284, 300, 1422, 1817
StyI CCWWGG 2 cut(s) 309, 474
TaaI ACNGT 2 cut(s) 1358, 1657
TaiI ACGT 3 cut(s) 213, 639, 2176
TaqI TCGA 5 cut(s) 269, 338, 407, 588, 923
TatI WGTACW 4 cut(s) 294, 480, 879, 1481
TauI GCSGC 1 cut(s) 190
TfiI GAWTC 8 cut(s) 274, 340, 404, 409, 530, 543, 906, 2159
TscAI CASTG 2 cut(s) 1774, 2155
TseFI GTSAC 1 cut(s) 1325
TseI GCWGC 5 cut(s) 319, 682, 1133, 1430, 2096
Tsp45I GTSAC 1 cut(s) 1325
TspGWI ACGGA 2 cut(s) 573, 1631
TspRI CASTG 2 cut(s) 1774, 2155
Tth111I GACNNNGTC 2 cut(s) 212, 329
Vha464I CTTAAG 2 cut(s) 194, 1198
VpaK11BI GGWCC 1 cut(s) 1181
XagI CCTNNNNNAGG 2 cut(s) 1424, 1822
XapI RAATTY 4 cut(s) 579, 1031, 1164, 1712
XceI RCATGY 1 cut(s) 2010
XmiI GTMKAC 2 cut(s) 856, 1457
XmnI GAANNNNTTC 1 cut(s) 344
XspI CTAG 3 cut(s) 323, 875, 1872
ZraI GACGTC 2 cut(s) 211, 637
ZrmI AGTACT 1 cut(s) 1483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.