FvH4_6g37551

Functions as component of the Arp2 3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0006841
Physical Location & Seq
Forward (+)
29574549 .. 29581360
6812 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g37551.t4

Sequence Viewer

Length: 1137 bp
ATGGCCGCGGTCGAAGTGCACCGTTTTGCTGAGTGCATCACTTGCCATGCTTGGAGCCCTGATCGCTCAATGCTGGCGTTTTGTCCAAATAACAATGAAGTTCACATCTATACTCTGATGCAATCCAAATGGGAAAAGCTGCATGTTCTTCAGAAGCATGACCAAATTATTTCGGGGATAGACTGGAGTTCAAGGTCAAATAAAATAGTTACTTCATCTCACGATCGGAATTCTTATGTCTGGAATCTAGAAGGATCAGAGTGGGTACCAACACTTGTTATCCTTCGGCTAAATCGTGCTGCACTGTGTGTTCAGTGGAGTCCAAAAGAAAACAAGTTTGCTGTCGGAAGCGGGGCAAAAACTGTTTGCATATGCTACTATGAGCAAGAGAATAACTGGTGGGTCAGTAAACTTATCAGAAAAAGACATGACTCTTCTGTTACAAGTGTTGCTTGGCATCCCAATAATATTCTTCTTGCAACAACATCTACAGATGGAAAATGCAGAATATTTTCCACTTATATTAAGGTCGTCGATGCAAAGGAATCAAAAACAGGTTCATCTTCAGATTCAAAGTTTGGGGAGCTAATACTTCAGCTTGATCTATCGTCTTGTTGGGCATTTGGTGTGAGGTGGTCACCAAGTGGCAACACCTTAGCATATGTAGGCCATAACTCTATGATTTACTTCGTCGATGAGGTGGGACCTTCTCCTTTGGCTCAAAATGTTGCATTCCGTGATTTGCCTCTCCGTGATGTCTTATTTCTGTCTGAGAGACTAGTCATTGGCGTGGGATTCGATTGCAACCCCATGGTTTTTGCAGCAGATGAAAGAGGAATATGGAGCTTTATCAGATTTCTTGGGGAAAGAAAGATTACATCTTCAGCTCCAAAATATGGTTCACAGCTTTCTGGAGCATTTCGGACGCTATATGGCCAACCAAAACATGGATTAAACAATGATACAAATGACCATTCAAGACCACAAGGAACTGTCCATGAGAACTGCATCAATTGTATTGTGCCGCTTGGAACTTCAGGGAGTTCTAAAGTAATGCGCTTCAGTACATCAGGATTGGACGGAAAAGTTGCTGTCTGGGATTTGGAGAATCAAGCAGACTTATCAGAGTATCTTTAG

Protein Analysis

379

Amino Acids

42.39

Weight (kDa)

7.58

Isoelectric Point (pI)

36.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_EML_2 PF23414 4 - 89 4.4e-13 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_EML PF23409 4 - 176 4.2e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_THOC3 PF25174 9 - 85 5.2e-07 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 9 - 174 3.2e-14 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 10 - 174 6.8e-21 WDR5 beta-propeller domain
Beta-prop_RIG_2nd PF23775 10 - 109 1.5e-07 RIG second beta-propeller
WD40_WDHD1_1st PF24817 11 - 87 3.8e-07 WDHD1 first WD40 domain
Beta-prop_WDR3_1st PF25173 11 - 85 2.8e-07 WDR3 first beta-propeller domain
Beta-prop_WDR19_1st PF23389 11 - 86 9.9e-06 WDR19 first beta-propeller
WD40_Gbeta PF25391 12 - 172 4.3e-07 G protein beta WD-40 repeat protein
Beta-prop_IFT140_1st PF23383 13 - 84 1e-05 IFT140 first beta-propeller
Beta-prop_WDR35_TULP_N PF24797 46 - 175 9.9e-06 WDR35/TULP4 N-terminal
WD40_Prp19 PF24814 64 - 171 6.7e-09 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 66 - 203 5.3e-12 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 79 - 172 3.1e-06 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 91 - 226 5.2e-08 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 100 - 232 3.4e-11 THOC3 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 104 - 186 4.1e-07 CAF1B/HIR1 beta-propeller domain
ANAPC4_WD40 PF12894 104 - 174 1.1e-06 Anaphase-promoting complex subunit 4 WD40 domain
WD40 PF00400 141 - 171 2.1e-07 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 265
AccB1I GGYRCC 1 cut(s) 265
AccB7I CCANNNNNTGG 2 cut(s) 896, 947
AccII CGCG 1 cut(s) 8
AciI CCGC 4 cut(s) 6, 8, 351, 1025
AclWI GGATC 1 cut(s) 262
AcoI YGGCCR 2 cut(s) 3, 934
AcsI RAATTY 1 cut(s) 229
AcuI CTGAAG 6 cut(s) 134, 549, 578, 867, 1020, 1045
AdeI CACNNNGTG 2 cut(s) 308, 644
AfaI GTAC 2 cut(s) 267, 1066
AfiI CCNNNNNNNGG 2 cut(s) 896, 947
AgsI TTSAA 3 cut(s) 192, 573, 978
AhlI ACTAGT 1 cut(s) 778
AjuI GAANNNNNNNTTGG 2 cut(s) 883, 915
AluBI AGCT 6 cut(s) 139, 586, 598, 846, 887, 907
AluI AGCT 6 cut(s) 139, 586, 598, 846, 887, 907
Alw21I GWGCWC 1 cut(s) 21
Alw26I GTCTC 1 cut(s) 769
Alw44I GTGCAC 1 cut(s) 17
AlwI GGATC 1 cut(s) 262
AoxI GGCC 3 cut(s) 3, 667, 934
ApaLI GTGCAC 1 cut(s) 17
ApeKI GCWGC 3 cut(s) 139, 299, 821
ApoI RAATTY 1 cut(s) 229
Asp700I GAANNNNTTC 1 cut(s) 511
Asp718I GGTACC 1 cut(s) 265
AspLEI GCGC 1 cut(s) 1059
AspS9I GGNCC 1 cut(s) 704
AsuHPI GGTGA 1 cut(s) 630
AvaII GGWCC 1 cut(s) 704
BaeGI GKGCMC 1 cut(s) 21
BalI TGGCCA 1 cut(s) 936
BanI GGYRCC 1 cut(s) 265
BanII GRGCYC 1 cut(s) 59
Bbv12I GWGCWC 1 cut(s) 21
BbvI GCAGC 3 cut(s) 126, 286, 833
BccI CCATC 1 cut(s) 488
BcoDI GTCTC 1 cut(s) 769
BcuI ACTAGT 1 cut(s) 778
BfaI CTAG 2 cut(s) 248, 779
BfmI CTRYAG 1 cut(s) 489
BisI GCNGC 5 cut(s) 6, 140, 300, 822, 1025
BlsI GCNGC 5 cut(s) 7, 141, 301, 823, 1026
Bme18I GGWCC 1 cut(s) 704
BmgT120I GGNCC 1 cut(s) 704
BmiI GGNNCC 3 cut(s) 56, 267, 705
BmsI GCATC 5 cut(s) 45, 108, 466, 526, 1017
BpmI CTGGAG 2 cut(s) 205, 933
Bpu10I CCTNAGC 1 cut(s) 655
BsaJI CCNNGG 2 cut(s) 6, 810
Bsc4I CCNNNNNNNGG 2 cut(s) 896, 947
Bse1I ACTGG 2 cut(s) 188, 401
BseDI CCNNGG 2 cut(s) 6, 810
BseGI GGATG 1 cut(s) 457
BseLI CCNNNNNNNGG 2 cut(s) 896, 947
BseMII CTCAG 2 cut(s) 21, 762
BseNI ACTGG 2 cut(s) 188, 401
BseSI GKGCMC 1 cut(s) 21
BseXI GCAGC 3 cut(s) 126, 286, 833
BsgI GTGCAG 1 cut(s) 285
Bsh1236I CGCG 1 cut(s) 8
Bsh1285I CGRYCG 2 cut(s) 12, 226
BshFI GGCC 3 cut(s) 5, 669, 936
BshNI GGYRCC 1 cut(s) 265
BsiEI CGRYCG 2 cut(s) 12, 226
BsiHKAI GWGCWC 1 cut(s) 21
BslFI GGGAC 1 cut(s) 717
BslI CCNNNNNNNGG 2 cut(s) 896, 947
BsmAI GTCTC 1 cut(s) 769
BsmFI GGGAC 1 cut(s) 717
BsmI GAATGC 1 cut(s) 731
BsnI GGCC 3 cut(s) 5, 669, 936
Bsp1286I GDGCHC 2 cut(s) 21, 59
Bsp143I GATC 4 cut(s) 61, 223, 254, 601
Bsp19I CCATGG 1 cut(s) 810
BspACI CCGC 4 cut(s) 6, 8, 351, 1025
BspANI GGCC 3 cut(s) 5, 669, 936
BspCNI CTCAG 2 cut(s) 22, 763
BspFNI CGCG 1 cut(s) 8
BspLI GGNNCC 3 cut(s) 56, 267, 705
BspPI GGATC 1 cut(s) 262
BspT107I GGYRCC 1 cut(s) 265
BsrI ACTGG 2 cut(s) 188, 401
BssECI CCNNGG 2 cut(s) 6, 810
BssMI GATC 4 cut(s) 61, 223, 254, 601
BssT1I CCWWGG 1 cut(s) 810
Bst4CI ACNGT 4 cut(s) 23, 306, 364, 994
Bst6I CTCTTC 1 cut(s) 439
BstAPI GCANNNNNTGC 1 cut(s) 42
BstC8I GCNNGC 1 cut(s) 75
BstDEI CTNAG 3 cut(s) 30, 655, 771
BstDSI CCRYGG 2 cut(s) 6, 810
BstEII GGTNACC 1 cut(s) 636
BstF5I GGATG 1 cut(s) 457
BstFNI CGCG 1 cut(s) 8
BstHHI GCGC 1 cut(s) 1059
BstKTI GATC 4 cut(s) 64, 226, 257, 604
BstMAI GTCTC 1 cut(s) 769
BstMBI GATC 4 cut(s) 61, 223, 254, 601
BstMCI CGRYCG 2 cut(s) 12, 226
BstMWI GCNNNNNNNGC 2 cut(s) 42, 63
BstNSI RCATGY 1 cut(s) 146
BstPI GGTNACC 1 cut(s) 636
BstSFI CTRYAG 1 cut(s) 489
BstSLI GKGCMC 1 cut(s) 21
BstUI CGCG 1 cut(s) 8
BstV1I GCAGC 3 cut(s) 126, 286, 833
BsuRI GGCC 3 cut(s) 5, 669, 936
BtgI CCRYGG 2 cut(s) 6, 810
BtsCI GGATG 1 cut(s) 457
BtsIMutI CAGTG 2 cut(s) 302, 320
Cac8I GCNNGC 1 cut(s) 75
CfoI GCGC 1 cut(s) 1059
Cfr13I GGNCC 1 cut(s) 704
Cfr42I CCGCGG 1 cut(s) 9
CseI GACGC 1 cut(s) 934
Csp6I GTAC 2 cut(s) 266, 1065
CviAII CATG 7 cut(s) 47, 143, 158, 428, 811, 947, 998
CviQI GTAC 2 cut(s) 266, 1065
DdeI CTNAG 3 cut(s) 30, 655, 771
DpnI GATC 4 cut(s) 63, 225, 256, 603
DpnII GATC 4 cut(s) 61, 223, 254, 601
DraIII CACNNNGTG 2 cut(s) 308, 644
EaeI YGGCCR 2 cut(s) 3, 934
Eam1104I CTCTTC 1 cut(s) 439
EarI CTCTTC 1 cut(s) 439
Eco130I CCWWGG 1 cut(s) 810
Eco24I GRGCYC 1 cut(s) 59
Eco47I GGWCC 1 cut(s) 704
Eco57I CTGAAG 6 cut(s) 134, 549, 578, 867, 1020, 1045
Eco91I GGTNACC 1 cut(s) 636
EcoO109I RGGNCCY 1 cut(s) 704
EcoO65I GGTNACC 1 cut(s) 636
EcoRI GAATTC 1 cut(s) 229
EcoT14I CCWWGG 1 cut(s) 810
EcoT38I GRGCYC 1 cut(s) 59
ErhI CCWWGG 1 cut(s) 810
FaeI CATG 7 cut(s) 50, 146, 161, 431, 814, 950, 1001
FalI AAGNNNNNCTT 2 cut(s) 436, 468
FaqI GGGAC 1 cut(s) 717
FatI CATG 7 cut(s) 46, 142, 157, 427, 810, 946, 997
FauI CCCGC 1 cut(s) 344
FauNDI CATATG 2 cut(s) 371, 661
Fnu4HI GCNGC 5 cut(s) 6, 140, 300, 822, 1025
FokI GGATG 1 cut(s) 444
FriOI GRGCYC 1 cut(s) 59
Fsp4HI GCNGC 5 cut(s) 6, 140, 300, 822, 1025
FspBI CTAG 2 cut(s) 248, 779
GlaI GCGC 1 cut(s) 1058
GluI GCNGC 5 cut(s) 6, 140, 300, 822, 1025
GsuI CTGGAG 2 cut(s) 205, 933
HaeIII GGCC 3 cut(s) 5, 669, 936
HgaI GACGC 1 cut(s) 934
HhaI GCGC 1 cut(s) 1059
Hin1II CATG 7 cut(s) 50, 146, 161, 431, 814, 950, 1001
Hin6I GCGC 1 cut(s) 1057
HinP1I GCGC 1 cut(s) 1057
HinfI GANTC 7 cut(s) 244, 319, 431, 545, 569, 795, 1108
HphI GGTGA 1 cut(s) 630
Hpy166II GTNNAC 4 cut(s) 19, 103, 410, 902
Hpy188III TCNNGA 6 cut(s) 221, 241, 248, 912, 978, 1071
Hpy8I GTNNAC 4 cut(s) 19, 103, 410, 902
Hpy99I CGWCG 2 cut(s) 536, 695
HpyAV CCTTC 3 cut(s) 245, 293, 717
HpyCH4III ACNGT 4 cut(s) 23, 306, 364, 994
HpyF10VI GCNNNNNNNGC 2 cut(s) 42, 63
HpyF3I CTNAG 3 cut(s) 30, 655, 771
Hsp92II CATG 7 cut(s) 50, 146, 161, 431, 814, 950, 1001
HspAI GCGC 1 cut(s) 1057
KpnI GGTACC 1 cut(s) 269
KspI CCGCGG 1 cut(s) 9
Kzo9I GATC 4 cut(s) 61, 223, 254, 601
LmnI GCTCC 5 cut(s) 54, 583, 843, 892, 914
Lsp1109I GCAGC 3 cut(s) 126, 286, 833
LweI GCATC 5 cut(s) 45, 108, 466, 526, 1017
MaeI CTAG 2 cut(s) 248, 779
MaeIII GTNAC 3 cut(s) 208, 439, 636
MalI GATC 4 cut(s) 63, 225, 256, 603
MboI GATC 4 cut(s) 61, 223, 254, 601
MboII GAAGA 5 cut(s) 140, 426, 464, 555, 873
MfeI CAATTG 1 cut(s) 1012
MhlI GDGCHC 2 cut(s) 21, 59
MlsI TGGCCA 1 cut(s) 936
MluCI AATT 3 cut(s) 165, 229, 1012
MluNI TGGCCA 1 cut(s) 936
MlyI GAGTC 2 cut(s) 328, 425
MmeI TCCRAC 1 cut(s) 325
MnlI CCTC 4 cut(s) 624, 691, 756, 827
Mox20I TGGCCA 1 cut(s) 936
MroXI GAANNNNTTC 1 cut(s) 511
MscI TGGCCA 1 cut(s) 936
MseI TTAA 2 cut(s) 525, 953
MslI CAYNNNNRTG 1 cut(s) 788
Msp20I TGGCCA 1 cut(s) 936
MspA1I CMGCKG 1 cut(s) 8
MunI CAATTG 1 cut(s) 1012
Mva1269I GAATGC 1 cut(s) 731
MvnI CGCG 1 cut(s) 8
MwoI GCNNNNNNNGC 2 cut(s) 42, 63
NcoI CCATGG 1 cut(s) 810
NdeI CATATG 2 cut(s) 371, 661
NdeII GATC 4 cut(s) 61, 223, 254, 601
NlaIII CATG 7 cut(s) 50, 146, 161, 431, 814, 950, 1001
NlaIV GGNNCC 3 cut(s) 56, 267, 705
NmuCI GTSAC 1 cut(s) 636
NspI RCATGY 1 cut(s) 146
PcsI WCGNNNNNNNCGW 1 cut(s) 292
PctI GAATGC 1 cut(s) 731
PdmI GAANNNNTTC 1 cut(s) 511
PfeI GAWTC 5 cut(s) 244, 545, 569, 795, 1108
PflMI CCANNNNNTGG 2 cut(s) 896, 947
PkrI GCNGC 5 cut(s) 7, 141, 301, 823, 1026
Ple19I CGATCG 1 cut(s) 226
PleI GAGTC 2 cut(s) 327, 425
PpsI GAGTC 2 cut(s) 327, 425
PpuMI RGGWCCY 1 cut(s) 704
Psp5II RGGWCCY 1 cut(s) 704
PspEI GGTNACC 1 cut(s) 636
PspN4I GGNNCC 3 cut(s) 56, 267, 705
PspPI GGNCC 1 cut(s) 704
PspPPI RGGWCCY 1 cut(s) 704
PvuI CGATCG 1 cut(s) 226
RsaI GTAC 2 cut(s) 267, 1066
RsaNI GTAC 2 cut(s) 266, 1065
RseI CAYNNNNRTG 1 cut(s) 788
SacII CCGCGG 1 cut(s) 9
SaqAI TTAA 2 cut(s) 525, 953
SatI GCNGC 5 cut(s) 6, 140, 300, 822, 1025
Sau3AI GATC 4 cut(s) 61, 223, 254, 601
Sau96I GGNCC 1 cut(s) 704
SchI GAGTC 2 cut(s) 328, 425
SduI GDGCHC 2 cut(s) 21, 59
SfaNI GCATC 5 cut(s) 45, 108, 466, 526, 1017
SfcI CTRYAG 1 cut(s) 489
Sfr303I CCGCGG 1 cut(s) 9
SgrBI CCGCGG 1 cut(s) 9
SinI GGWCC 1 cut(s) 704
SmiMI CAYNNNNRTG 1 cut(s) 788
SpeI ACTAGT 1 cut(s) 778
Sse9I AATT 3 cut(s) 165, 229, 1012
SsiI CCGC 4 cut(s) 6, 8, 351, 1025
SspI AATATT 2 cut(s) 469, 510
SspMI CTAG 2 cut(s) 248, 779
StyI CCWWGG 1 cut(s) 810
TaaI ACNGT 4 cut(s) 23, 306, 364, 994
TaqI TCGA 4 cut(s) 12, 534, 693, 798
TasI AATT 3 cut(s) 165, 229, 1012
TatI WGTACW 1 cut(s) 1064
TauI GCSGC 2 cut(s) 8, 1027
TfiI GAWTC 5 cut(s) 244, 545, 569, 795, 1108
Tru1I TTAA 2 cut(s) 525, 953
Tru9I TTAA 2 cut(s) 525, 953
TscAI CASTG 2 cut(s) 309, 320
TseFI GTSAC 1 cut(s) 636
TseI GCWGC 3 cut(s) 139, 299, 821
Tsp45I GTSAC 1 cut(s) 636
TspDTI ATGAA 4 cut(s) 111, 204, 549, 843
TspGWI ACGGA 3 cut(s) 725, 740, 1095
TspRI CASTG 2 cut(s) 309, 320
Van91I CCANNNNNTGG 2 cut(s) 896, 947
VneI GTGCAC 1 cut(s) 17
VpaK11BI GGWCC 1 cut(s) 704
XapI RAATTY 1 cut(s) 229
XbaI TCTAGA 1 cut(s) 247
XceI RCATGY 1 cut(s) 146
XcmI CCANNNNNNNNNTGG 1 cut(s) 944
XmnI GAANNNNTTC 1 cut(s) 511
XspI CTAG 2 cut(s) 248, 779
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.