Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0006841

Physical Location & Seq

Genomic Coordinates Reverse (-)

12475773 .. 12482073

Gene Structure 6301 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1156800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1161 bp

ATGGCGGCGGTGGAAGTTCACAAGTTCGCTCAGTGCATCACTTGCCACGCTTGGAGCCGTGATCGCTCCATGCTCGCATTTTGTCCGAACAACAATGAAGTTCACATCTATGCTCTGTTGCAAGGAAAGTGGGAGAAGCTGCACGTTCTTCAGAAGCTTTGTTGGAAGGGATTACGTCAATTTCATGACCAAATTGTATCTGGGATAGACTGGAGCGCGAGGGCAAACAAAATAGTTACTGCATCTCATGATCGGAATTCTTATGTCTGGAATCTAGAAGGATCAGAGTGGGTACCAACCCTTGTTATCCTTCGGCTAAATCGTGCTGCACTTTGTGTTAAGTGGAATCCAAGAGAAAACAAGTTTGCTGTTGGAAGTGGGGCAAAAACTGTTTGTATATGCTACTACGAGCAAGAGAATAACTGGTGGGTCAGTAAACTTATCAGAAAAAGGCACGACTCTTCTGTGACAAGTGTTGCTTGGCATCCCAATAATATTCTTCTAGCAACAGCATCTACAGATGGAAAATGCCGTATATTTTCCACTTTTATTAAGGTCGTCGATGCAAAGGAATCAAGAACAGGCCCATTTGCTGATTCAAAATTTGGAGAGCAAATTCTTCAGCTTGATCTATCATTTTCTTGGTCATTTGGCGTGAGGTGGTCACCGAGTGGCAATACCTTAGCATATGTAGGCCATAGTTCTATGATTTACTTCGTTGATGATGTTGGACCTTCTCCTCTGGCTCAAAATGTTGCATTCCGTGATTTGCCTCTCCGTGATGTGTTATTTCTGTCTGAAAGATTACTTATAGGCGTGGGGTTTGATTGCAATCCAATGGTGTTTGCGGCAGATTTACATGGAATATGGCATTTTATCAAATTTCTTGGGGAAAGAAAAACATCTTCAGGTCCGAAATATGGTTCACAGTTTTCTGAAGCATTCGGAAAGCTGTATGGCCAACAAAAACATGGATTGAACAATGATGCAAATGAACATTCAAGATCACGAGGACGTGTCCATGAGAACTGCATCAATTGTATCGTGCCGCTTGGAGAACCAGGGAGTTCTAAAGTAACGCGCTTCAGCACATCAGGATTGGATGGAAAAGTAGTTGTGTGGGATATGGAGAACCAGGCAGATTTATCGGAGTATTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005856 GO:0005885 GO:0006996 GO:0007010 GO:0007015 GO:0008064 GO:0008092 GO:0008150 GO:0009987 GO:0010638 GO:0015629 GO:0016043 GO:0030029 GO:0030036 GO:0030832 GO:0030833 GO:0030838 GO:0031334 GO:0032271 GO:0032273 GO:0032535 GO:0032956 GO:0032970 GO:0032991 GO:0033043 GO:0034314 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043254 GO:0044087 GO:0044089 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0044877 GO:0045010 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051015 GO:0051128 GO:0051130 GO:0051493 GO:0051495 GO:0065007 GO:0065008 GO:0071840 GO:0090066 GO:0097435 GO:0110053 GO:1902903 GO:1902905

KEGG Pathways

Metabolic & Signaling

ko04144 ko04666 ko04810 ko05100 ko05130 ko05131 ko05132 map04144 map04666 map04810 map05100 map05130 map05131 map05132

Pfam Domains

Protein Families

ANAPC4_WD40 WD40

Protein Analysis

387

Amino Acids

43.59

Weight (kDa)

8.69

Isoelectric Point (pI)

33.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_EML_2	PF23414	4 - 92	6.3e-06	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5	PF25175	9 - 182	2e-17	WDR5 beta-propeller domain
WD40_Prp19	PF24814	62 - 180	1.8e-09	Prp19 WD40 domain
Beta-prop_EML_2	PF23414	68 - 182	1.6e-12	Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st	PF24817	99 - 182	8.4e-07	WDHD1 first WD40 domain
Beta-prop_THOC3	PF25174	109 - 241	6.4e-11	THOC3 beta-propeller domain
ANAPC4_WD40	PF12894	113 - 181	6.3e-06	Anaphase-promoting complex subunit 4 WD40 domain
WD40	PF00400	150 - 180	1.2e-07	WD domain, G-beta repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006841)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G30910 AT2G30910 AT2G30910 AT2G31300 AT2G31300
fragaria_vesca	FvH4_6g37551 FvH4_6g37551 FvH4_6g37551 FvH4_6g37551
malus_domestica	MD09G1156800.v1.1 MD17G1143300.v1.1
prunus_persica	Prupe.3G170000_v2.0.a1
pyrus_communis	pycom09g07550 pycom17g13640
rosa_chinensis	RchiOBHm_Chr2g0095271 RchiOBHm_Chr2g0150441
rosa_laevigata	RLG00000020499
rosa_multiflora	Rmu_co8083376.1_g000001 Rmu_sc0020041.1_g000002 Rmu_ssc0000480.1_g000018
rosa_roxburghii	Rroxscaffold_2G00097100 Rroxscaffold_6G00394160
rosa_rugosa	Rorug02G0421500
rosa_samantha	Rh2AG480700 Rh2DG501700
rosa_wichuraiana	Rw2G039250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	292
AccB1I	GGYRCC	1 cut(s)	292
AccII	CGCG	2 cut(s)	218, 1081
AciI	CCGC	4 cut(s)	5, 8, 848, 1049
AclWI	GGATC	1 cut(s)	289
AcoI	YGGCCR	1 cut(s)	958
AcsI	RAATTY	4 cut(s)	256, 602, 615, 881
AcuI	CTGAAG	5 cut(s)	134, 605, 891, 957, 1069
AdeI	CACNNNGTG	2 cut(s)	335, 671
AfaI	GTAC	1 cut(s)	294
AfiI	CCNNNNNNNGG	1 cut(s)	920
AflIII	ACRYGT	1 cut(s)	1015
AgsI	TTSAA	3 cut(s)	600, 979, 1002
AjiI	CACGTC	1 cut(s)	1016
AjnI	CCWGG	2 cut(s)	1060, 1134
AluBI	AGCT	4 cut(s)	139, 157, 625, 952
AluI	AGCT	4 cut(s)	139, 157, 625, 952
AlwI	GGATC	1 cut(s)	289
AoxI	GGCC	3 cut(s)	583, 694, 958
ApeKI	GCWGC	2 cut(s)	139, 326
ApoI	RAATTY	4 cut(s)	256, 602, 615, 881
Asp718I	GGTACC	1 cut(s)	292
AspLEI	GCGC	2 cut(s)	218, 1083
AspS9I	GGNCC	3 cut(s)	584, 731, 911
AsuHPI	GGTGA	1 cut(s)	657
AvaII	GGWCC	2 cut(s)	731, 911
BalI	TGGCCA	1 cut(s)	960
BanI	GGYRCC	1 cut(s)	292
BauI	CACGAG	1 cut(s)	1008
BbvI	GCAGC	2 cut(s)	126, 313
BccI	CCATC	2 cut(s)	515, 1097
BceAI	ACGGC	2 cut(s)	42, 516
BcgI	CGANNNNNNTGC	1 cut(s)	1128
BciT130I	CCWGG	2 cut(s)	1062, 1136
BfaI	CTAG	2 cut(s)	275, 503
BfmI	CTRYAG	1 cut(s)	516
BisI	GCNGC	5 cut(s)	6, 140, 327, 849, 1049
BlsI	GCNGC	5 cut(s)	7, 141, 328, 850, 1050
Bme1390I	CCNGG	2 cut(s)	1062, 1136
Bme18I	GGWCC	2 cut(s)	731, 911
BmgBI	CACGTC	1 cut(s)	1016
BmgT120I	GGNCC	3 cut(s)	584, 731, 911
BmiI	GGNNCC	2 cut(s)	56, 294
BmrFI	CCNGG	2 cut(s)	1062, 1136
BmsI	GCATC	7 cut(s)	45, 251, 493, 521, 553, 976, 1041
BpmI	CTGGAG	1 cut(s)	232
Bpu10I	CCTNAGC	1 cut(s)	682
BsaBI	GATNNNNATC	1 cut(s)	831
BsaJI	CCNNGG	1 cut(s)	1061
BsaXI	ACNNNNNCTCC	1 cut(s)	1142
Bsc4I	CCNNNNNNNGG	1 cut(s)	920
Bse1I	ACTGG	2 cut(s)	215, 428
Bse8I	GATNNNNATC	1 cut(s)	831
BseBI	CCWGG	2 cut(s)	1062, 1136
BseDI	CCNNGG	1 cut(s)	1061
BseGI	GGATG	2 cut(s)	484, 1108
BseJI	GATNNNNATC	1 cut(s)	831
BseLI	CCNNNNNNNGG	1 cut(s)	920
BseMII	CTCAG	1 cut(s)	44
BseNI	ACTGG	2 cut(s)	215, 428
BseRI	GAGGAG	1 cut(s)	729
BseXI	GCAGC	2 cut(s)	126, 313
BsgI	GTGCAG	2 cut(s)	125, 312
Bsh1236I	CGCG	2 cut(s)	218, 1081
BshFI	GGCC	3 cut(s)	585, 696, 960
BshNI	GGYRCC	1 cut(s)	292
BslI	CCNNNNNNNGG	1 cut(s)	920
BsmI	GAATGC	2 cut(s)	758, 941
BsnI	GGCC	3 cut(s)	585, 696, 960
Bsp143I	GATC	5 cut(s)	61, 250, 281, 628, 1004
BspACI	CCGC	4 cut(s)	5, 8, 848, 1049
BspANI	GGCC	3 cut(s)	585, 696, 960
BspCNI	CTCAG	1 cut(s)	43
BspFNI	CGCG	2 cut(s)	218, 1081
BspHI	TCATGA	2 cut(s)	184, 247
BspLI	GGNNCC	2 cut(s)	56, 294
BspPI	GGATC	1 cut(s)	289
BspT107I	GGYRCC	1 cut(s)	292
BsrI	ACTGG	2 cut(s)	215, 428
BssECI	CCNNGG	1 cut(s)	1061
BssMI	GATC	5 cut(s)	61, 250, 281, 628, 1004
BssSI	CACGAG	1 cut(s)	1008
Bst2BI	CACGAG	1 cut(s)	1008
Bst2UI	CCWGG	2 cut(s)	1062, 1136
Bst4CI	ACNGT	2 cut(s)	391, 930
Bst6I	CTCTTC	1 cut(s)	466
BstAPI	GCANNNNNTGC	1 cut(s)	42
BstC8I	GCNNGC	1 cut(s)	75
BstDEI	CTNAG	2 cut(s)	30, 682
BstEII	GGTNACC	1 cut(s)	663
BstF5I	GGATG	2 cut(s)	484, 1108
BstFNI	CGCG	2 cut(s)	218, 1081
BstHHI	GCGC	2 cut(s)	218, 1083
BstKTI	GATC	5 cut(s)	64, 253, 284, 631, 1007
BstMBI	GATC	5 cut(s)	61, 250, 281, 628, 1004
BstMWI	GCNNNNNNNGC	2 cut(s)	42, 63
BstNI	CCWGG	2 cut(s)	1062, 1136
BstPI	GGTNACC	1 cut(s)	663
BstSCI	CCNGG	2 cut(s)	1060, 1134
BstSFI	CTRYAG	1 cut(s)	516
BstUI	CGCG	2 cut(s)	218, 1081
BstV1I	GCAGC	2 cut(s)	126, 313
BsuRI	GGCC	3 cut(s)	585, 696, 960
BtrI	CACGTC	1 cut(s)	1016
BtsCI	GGATG	2 cut(s)	484, 1108
BtsIMutI	CAGTG	1 cut(s)	38
Cac8I	GCNNGC	1 cut(s)	75
CciI	TCATGA	2 cut(s)	184, 247
CfoI	GCGC	2 cut(s)	218, 1083
Cfr13I	GGNCC	3 cut(s)	584, 731, 911
Csp6I	GTAC	1 cut(s)	293
CspCI	CAANNNNNGTGG	2 cut(s)	110, 145
CviAII	CATG	6 cut(s)	70, 185, 248, 860, 971, 1022
CviQI	GTAC	1 cut(s)	293
DdeI	CTNAG	2 cut(s)	30, 682
DpnI	GATC	5 cut(s)	63, 252, 283, 630, 1006
DpnII	GATC	5 cut(s)	61, 250, 281, 628, 1004
DraIII	CACNNNGTG	2 cut(s)	335, 671
EaeI	YGGCCR	1 cut(s)	958
Eam1104I	CTCTTC	1 cut(s)	466
EarI	CTCTTC	1 cut(s)	466
Eco47I	GGWCC	2 cut(s)	731, 911
Eco57I	CTGAAG	5 cut(s)	134, 605, 891, 957, 1069
Eco91I	GGTNACC	1 cut(s)	663
EcoO65I	GGTNACC	1 cut(s)	663
EcoRI	GAATTC	1 cut(s)	256
EcoRII	CCWGG	2 cut(s)	1060, 1134
FaeI	CATG	6 cut(s)	73, 188, 251, 863, 974, 1025
FalI	AAGNNNNNCTT	2 cut(s)	463, 495
FatI	CATG	6 cut(s)	69, 184, 247, 859, 970, 1021
FauNDI	CATATG	1 cut(s)	688
Fnu4HI	GCNGC	5 cut(s)	6, 140, 327, 849, 1049
FokI	GGATG	2 cut(s)	471, 1115
Fsp4HI	GCNGC	5 cut(s)	6, 140, 327, 849, 1049
FspBI	CTAG	2 cut(s)	275, 503
GlaI	GCGC	2 cut(s)	217, 1082
GluI	GCNGC	5 cut(s)	6, 140, 327, 849, 1049
GsuI	CTGGAG	1 cut(s)	232
HaeIII	GGCC	3 cut(s)	585, 696, 960
HhaI	GCGC	2 cut(s)	218, 1083
Hin1II	CATG	6 cut(s)	73, 188, 251, 863, 974, 1025
Hin6I	GCGC	2 cut(s)	216, 1081
HinP1I	GCGC	2 cut(s)	216, 1081
HindIII	AAGCTT	1 cut(s)	155
HinfI	GANTC	5 cut(s)	271, 346, 458, 572, 596
HphI	GGTGA	1 cut(s)	657
Hpy166II	GTNNAC	4 cut(s)	19, 103, 437, 926
Hpy188III	TCNNGA	8 cut(s)	185, 248, 268, 275, 576, 1002, 1008, 1095
Hpy8I	GTNNAC	4 cut(s)	19, 103, 437, 926
Hpy99I	CGWCG	1 cut(s)	563
HpyAV	CCTTC	4 cut(s)	160, 272, 320, 744
HpyCH4III	ACNGT	2 cut(s)	391, 930
HpyCH4IV	ACGT	3 cut(s)	144, 175, 1015
HpyF10VI	GCNNNNNNNGC	2 cut(s)	42, 63
HpyF3I	CTNAG	2 cut(s)	30, 682
HpySE526I	ACGT	3 cut(s)	144, 175, 1015
Hsp92II	CATG	6 cut(s)	73, 188, 251, 863, 974, 1025
HspAI	GCGC	2 cut(s)	216, 1081
KpnI	GGTACC	1 cut(s)	296
Kzo9I	GATC	5 cut(s)	61, 250, 281, 628, 1004
LmnI	GCTCC	3 cut(s)	54, 71, 213
Lsp1109I	GCAGC	2 cut(s)	126, 313
LweI	GCATC	7 cut(s)	45, 251, 493, 521, 553, 976, 1041
MaeI	CTAG	2 cut(s)	275, 503
MaeII	ACGT	3 cut(s)	144, 175, 1015
MaeIII	GTNAC	4 cut(s)	235, 466, 663, 1075
MalI	GATC	5 cut(s)	63, 252, 283, 630, 1006
MboI	GATC	5 cut(s)	61, 250, 281, 628, 1004
MboII	GAAGA	5 cut(s)	140, 453, 491, 611, 897
MfeI	CAATTG	1 cut(s)	1036
MlsI	TGGCCA	1 cut(s)	960
MluCI	AATT	7 cut(s)	179, 192, 256, 602, 615, 881, 1036
MluNI	TGGCCA	1 cut(s)	960
MlyI	GAGTC	1 cut(s)	452
MmeI	TCCRAC	3 cut(s)	143, 352, 709
MnlI	CCTC	5 cut(s)	213, 651, 750, 783, 1004
Mox20I	TGGCCA	1 cut(s)	960
MscI	TGGCCA	1 cut(s)	960
MseI	TTAA	2 cut(s)	339, 552
MslI	CAYNNNNRTG	1 cut(s)	108
Msp20I	TGGCCA	1 cut(s)	960
MspR9I	CCNGG	2 cut(s)	1062, 1136
MunI	CAATTG	1 cut(s)	1036
Mva1269I	GAATGC	2 cut(s)	758, 941
MvaI	CCWGG	2 cut(s)	1062, 1136
MvnI	CGCG	2 cut(s)	218, 1081
MwoI	GCNNNNNNNGC	2 cut(s)	42, 63
NdeI	CATATG	1 cut(s)	688
NdeII	GATC	5 cut(s)	61, 250, 281, 628, 1004
NlaIII	CATG	6 cut(s)	73, 188, 251, 863, 974, 1025
NlaIV	GGNNCC	2 cut(s)	56, 294
NmuCI	GTSAC	2 cut(s)	466, 663
PagI	TCATGA	2 cut(s)	184, 247
PcsI	WCGNNNNNNNCGW	1 cut(s)	319
PctI	GAATGC	2 cut(s)	758, 941
PfeI	GAWTC	4 cut(s)	271, 346, 572, 596
PkrI	GCNGC	5 cut(s)	7, 141, 328, 850, 1050
PleI	GAGTC	1 cut(s)	452
PpsI	GAGTC	1 cut(s)	452
Psp6I	CCWGG	2 cut(s)	1060, 1134
PspEI	GGTNACC	1 cut(s)	663
PspGI	CCWGG	2 cut(s)	1060, 1134
PspN4I	GGNNCC	2 cut(s)	56, 294
PspPI	GGNCC	3 cut(s)	584, 731, 911
RsaI	GTAC	1 cut(s)	294
RsaNI	GTAC	1 cut(s)	293
RseI	CAYNNNNRTG	1 cut(s)	108
SaqAI	TTAA	2 cut(s)	339, 552
SatI	GCNGC	5 cut(s)	6, 140, 327, 849, 1049
Sau3AI	GATC	5 cut(s)	61, 250, 281, 628, 1004
Sau96I	GGNCC	3 cut(s)	584, 731, 911
SchI	GAGTC	1 cut(s)	452
ScrFI	CCNGG	2 cut(s)	1062, 1136
SfaNI	GCATC	7 cut(s)	45, 251, 493, 521, 553, 976, 1041
SfcI	CTRYAG	1 cut(s)	516
SinI	GGWCC	2 cut(s)	731, 911
SmiMI	CAYNNNNRTG	1 cut(s)	108
Sse9I	AATT	7 cut(s)	179, 192, 256, 602, 615, 881, 1036
SsiI	CCGC	4 cut(s)	5, 8, 848, 1049
SspI	AATATT	1 cut(s)	496
SspMI	CTAG	2 cut(s)	275, 503
StyD4I	CCNGG	2 cut(s)	1060, 1134
TaaI	ACNGT	2 cut(s)	391, 930
TaiI	ACGT	3 cut(s)	147, 178, 1018
TaqI	TCGA	1 cut(s)	561
TasI	AATT	7 cut(s)	179, 192, 256, 602, 615, 881, 1036
TauI	GCSGC	3 cut(s)	8, 851, 1051
TfiI	GAWTC	4 cut(s)	271, 346, 572, 596
Tru1I	TTAA	2 cut(s)	339, 552
Tru9I	TTAA	2 cut(s)	339, 552
TscAI	CASTG	1 cut(s)	38
TseFI	GTSAC	2 cut(s)	466, 663
TseI	GCWGC	2 cut(s)	139, 326
Tsp45I	GTSAC	2 cut(s)	466, 663
TspDTI	ATGAA	3 cut(s)	111, 173, 1008
TspGWI	ACGGA	2 cut(s)	752, 767
TspRI	CASTG	1 cut(s)	38
VpaK11BI	GGWCC	2 cut(s)	731, 911
XapI	RAATTY	4 cut(s)	256, 602, 615, 881
XbaI	TCTAGA	1 cut(s)	274
XcmI	CCANNNNNNNNNTGG	2 cut(s)	197, 968
XspI	CTAG	2 cut(s)	275, 503

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.