Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0006841

Physical Location & Seq

Genomic Coordinates Forward (+)

64222173 .. 64228272

Gene Structure 6100 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G039250.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1137 bp

ATGGCCGCGGTCGAAGTGCACCGTTTTGCTCAGTGCATCACTTGCCATGCTTGGAGCCCTGATCGCTCAATGCTGGCATTTTGCCCAAATAACAATGAGGTTCACATCTATACTCTGATGCAATCCAAATGGGAAAAGCTGCATGTTCTTCAGAAGCATGACCAAATTATTTCTGGGATAGACTGGAGTGCAAGGTCAAACAAAATAGTTACTTCATCTCATGATCGGAATTCTTATGTCTGGAACCTAGAAGGATCAGAGTGGGTACCAACACTTGTCATCCTCCGGCTAAATCGTGCTGCACTTTGTGTTCAGTGGAGTCCAAAAGAAAACAAGTTTGCTGTAGGAAGTGGGGCAAAAACTGTTTGCATATGCTACTATGAGCAAGAGAACAACTGGTGGGTCAGTAAACTGATCAGAAAAAGACACGACTCTTCTGTTACAAGTGTTGCTTGGCATCCCAATAATATTCTTCTTGCAACAGCATCTACAGATGGAAAATGCCGAATATTTTCCACTTACATTAAGGTCGTCGATGCAAAGGAATCGAAAACAGTCTCATCTTCAGATGCAAAGTTTGGAGAGCTAATTCTTCAGCTTGATCTATTGTCTTCTTGGGCATTTGGTGTGAGGTGGTCACCAAGTGGCAATACCTTAGCATATGTAGGCCATAACTCTATGATTTACTTTGTCGATGAGGTGGGGCCTTCTCCTCTGGCTCAAAATGTTGCATTCCGTGATTTGCCTCTCCGTGATGTCTTATTTCTTTCTGAACGACTAGTCATTGGTGTGGGATTTGATTGCAACCCCATGGTTTTTGCAGCAGATGAAAGAGGAATATGGAGTTTTATAAGATTTCTTGGGGAAAGAAGAATTACTTCTTCAGGTCCAAAATATGGTTCACAGCTTTCTGGAGCATTTCGGACGCTATATGGCCAACCAAAGCATGGATTGAGCAATGATACAAATGACCATTCAAGACCACAAGGAACCGTCCATGAAAACTGCATTAATTGTATTGTGCCGCTTGGAACTTCAGGGAGTTCTAAAGTAATGCGCTTCAGCACATCAGGATTGGATGGAAAAGTCGTTGTCTGGGATCTGGAGAATCAAGCAGACTTATCAGAGTATTTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005856 GO:0005885 GO:0006996 GO:0007010 GO:0007015 GO:0008064 GO:0008092 GO:0008150 GO:0009987 GO:0010638 GO:0015629 GO:0016043 GO:0030029 GO:0030036 GO:0030832 GO:0030833 GO:0030838 GO:0031334 GO:0032271 GO:0032273 GO:0032535 GO:0032956 GO:0032970 GO:0032991 GO:0033043 GO:0034314 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043254 GO:0044087 GO:0044089 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0044877 GO:0045010 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051015 GO:0051128 GO:0051130 GO:0051493 GO:0051495 GO:0065007 GO:0065008 GO:0071840 GO:0090066 GO:0097435 GO:0110053 GO:1902903 GO:1902905

KEGG Pathways

Metabolic & Signaling

ko04144 ko04666 ko04810 ko05100 ko05130 ko05131 ko05132 map04144 map04666 map04810 map05100 map05130 map05131 map05132

Pfam Domains

Protein Families

ANAPC4_WD40 WD40

Protein Analysis

378

Amino Acids

42.43

Weight (kDa)

8.01

Isoelectric Point (pI)

33.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_EML	PF23409	4 - 176	5.9e-06	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_EML_2	PF23414	4 - 88	8.8e-13	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_THOC3	PF25174	7 - 86	8e-07	THOC3 beta-propeller domain
Beta-prop_RIG_2nd	PF23775	9 - 117	1.9e-08	RIG second beta-propeller
WD40_CDC20-Fz	PF24807	10 - 130	3.1e-10	CDC20/Fizzy WD40 domain
Beta-prop_WDR5	PF25175	10 - 173	1.1e-20	WDR5 beta-propeller domain
WD40_WDHD1_1st	PF24817	11 - 87	6.7e-07	WDHD1 first WD40 domain
Beta-prop_WDR3_1st	PF25173	11 - 85	5.2e-07	WDR3 first beta-propeller domain
WD40_Prp19	PF24814	11 - 172	2.8e-11	Prp19 WD40 domain
WD40_Gbeta	PF25391	11 - 172	6.3e-07	G protein beta WD-40 repeat protein
Beta-prop_EML_2	PF23414	67 - 184	6.1e-12	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st	PF25173	79 - 172	2.9e-06	WDR3 first beta-propeller domain
WD40_WDHD1_1st	PF24817	90 - 227	3.3e-08	WDHD1 first WD40 domain
Beta-prop_THOC3	PF25174	100 - 232	7.9e-12	THOC3 beta-propeller domain
Beta-prop_CAF1B_HIR1	PF24105	104 - 189	2.2e-07	CAF1B/HIR1 beta-propeller domain
ANAPC4_WD40	PF12894	104 - 174	4.7e-07	Anaphase-promoting complex subunit 4 WD40 domain
WD40	PF00400	141 - 171	1.2e-07	WD domain, G-beta repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0006841)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G30910 AT2G30910 AT2G30910 AT2G31300 AT2G31300
fragaria_vesca	FvH4_6g37551 FvH4_6g37551 FvH4_6g37551 FvH4_6g37551
malus_domestica	MD09G1156800.v1.1 MD17G1143300.v1.1
prunus_persica	Prupe.3G170000_v2.0.a1
pyrus_communis	pycom09g07550 pycom17g13640
rosa_chinensis	RchiOBHm_Chr2g0095271 RchiOBHm_Chr2g0150441
rosa_laevigata	RLG00000020499
rosa_multiflora	Rmu_co8083376.1_g000001 Rmu_sc0020041.1_g000002 Rmu_ssc0000480.1_g000018
rosa_roxburghii	Rroxscaffold_2G00097100 Rroxscaffold_6G00394160
rosa_rugosa	Rorug02G0421500
rosa_samantha	Rh2AG480700 Rh2DG501700
rosa_wichuraiana	Rw2G039250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	851
Acc65I	GGTACC	1 cut(s)	265
AccB1I	GGYRCC	1 cut(s)	265
AccB7I	CCANNNNNTGG	2 cut(s)	896, 947
AccII	CGCG	1 cut(s)	8
AciI	CCGC	3 cut(s)	6, 8, 1025
AclWI	GGATC	2 cut(s)	262, 1107
AcoI	YGGCCR	2 cut(s)	3, 934
AcsI	RAATTY	1 cut(s)	229
AcuI	CTGAAG	6 cut(s)	134, 549, 578, 867, 1020, 1045
AdeI	CACNNNGTG	2 cut(s)	308, 644
AfaI	GTAC	1 cut(s)	267
AfiI	CCNNNNNNNGG	2 cut(s)	896, 947
AgsI	TTSAA	1 cut(s)	978
AhlI	ACTAGT	1 cut(s)	778
AjuI	GAANNNNNNNTTGG	2 cut(s)	883, 915
AluBI	AGCT	4 cut(s)	139, 586, 598, 907
AluI	AGCT	4 cut(s)	139, 586, 598, 907
Alw21I	GWGCWC	1 cut(s)	21
Alw26I	GTCTC	1 cut(s)	562
Alw44I	GTGCAC	1 cut(s)	17
AlwI	GGATC	2 cut(s)	262, 1107
AoxI	GGCC	4 cut(s)	3, 667, 704, 934
ApaLI	GTGCAC	1 cut(s)	17
ApeKI	GCWGC	3 cut(s)	139, 299, 821
ApoI	RAATTY	1 cut(s)	229
AseI	ATTAAT	1 cut(s)	1011
Asp700I	GAANNNNTTC	2 cut(s)	511, 877
Asp718I	GGTACC	1 cut(s)	265
AspLEI	GCGC	1 cut(s)	1059
AspS9I	GGNCC	2 cut(s)	704, 887
AsuHPI	GGTGA	1 cut(s)	630
AvaII	GGWCC	1 cut(s)	887
BaeGI	GKGCMC	1 cut(s)	21
BalI	TGGCCA	1 cut(s)	936
BanI	GGYRCC	1 cut(s)	265
BanII	GRGCYC	1 cut(s)	59
BbsI	GAAGAC	1 cut(s)	603
Bbv12I	GWGCWC	1 cut(s)	21
BbvI	GCAGC	3 cut(s)	126, 286, 833
BccI	CCATC	2 cut(s)	488, 1073
BcgI	CGANNNNNNTGC	2 cut(s)	528, 562
BclI	TGATCA	1 cut(s)	414
BcoDI	GTCTC	1 cut(s)	562
BcuI	ACTAGT	1 cut(s)	778
BfaI	CTAG	2 cut(s)	248, 779
BfmI	CTRYAG	2 cut(s)	342, 489
BisI	GCNGC	5 cut(s)	6, 140, 300, 822, 1025
BlsI	GCNGC	5 cut(s)	7, 141, 301, 823, 1026
Bme18I	GGWCC	1 cut(s)	887
BmgT120I	GGNCC	2 cut(s)	704, 887
BmiI	GGNNCC	5 cut(s)	56, 245, 267, 705, 991
BmsI	GCATC	6 cut(s)	45, 108, 466, 494, 526, 559
BpiI	GAAGAC	1 cut(s)	603
BpmI	CTGGAG	3 cut(s)	205, 933, 1124
Bpu10I	CCTNAGC	1 cut(s)	655
BsaJI	CCNNGG	2 cut(s)	6, 810
Bsc4I	CCNNNNNNNGG	2 cut(s)	896, 947
Bse1I	ACTGG	2 cut(s)	188, 401
Bse3DI	GCAATG	1 cut(s)	964
BseDI	CCNNGG	2 cut(s)	6, 810
BseGI	GGATG	3 cut(s)	279, 457, 1084
BseLI	CCNNNNNNNGG	2 cut(s)	896, 947
BseMI	GCAATG	1 cut(s)	964
BseMII	CTCAG	1 cut(s)	44
BseNI	ACTGG	2 cut(s)	188, 401
BseRI	GAGGAG	1 cut(s)	702
BseSI	GKGCMC	1 cut(s)	21
BseXI	GCAGC	3 cut(s)	126, 286, 833
BsgI	GTGCAG	1 cut(s)	285
Bsh1236I	CGCG	1 cut(s)	8
Bsh1285I	CGRYCG	1 cut(s)	12
BshFI	GGCC	4 cut(s)	5, 669, 706, 936
BshNI	GGYRCC	1 cut(s)	265
BsiEI	CGRYCG	1 cut(s)	12
BsiHKAI	GWGCWC	1 cut(s)	21
BsiSI	CCGG	1 cut(s)	286
BslI	CCNNNNNNNGG	2 cut(s)	896, 947
BsmAI	GTCTC	1 cut(s)	562
BsmI	GAATGC	1 cut(s)	731
BsnI	GGCC	4 cut(s)	5, 669, 706, 936
Bsp1286I	GDGCHC	2 cut(s)	21, 59
Bsp143I	GATC	6 cut(s)	61, 223, 254, 414, 601, 1099
Bsp19I	CCATGG	1 cut(s)	810
BspACI	CCGC	3 cut(s)	6, 8, 1025
BspANI	GGCC	4 cut(s)	5, 669, 706, 936
BspCNI	CTCAG	1 cut(s)	43
BspFNI	CGCG	1 cut(s)	8
BspHI	TCATGA	1 cut(s)	220
BspLI	GGNNCC	5 cut(s)	56, 245, 267, 705, 991
BspPI	GGATC	2 cut(s)	262, 1107
BspT107I	GGYRCC	1 cut(s)	265
BsrDI	GCAATG	1 cut(s)	964
BsrI	ACTGG	2 cut(s)	188, 401
BssECI	CCNNGG	2 cut(s)	6, 810
BssMI	GATC	6 cut(s)	61, 223, 254, 414, 601, 1099
BssT1I	CCWWGG	1 cut(s)	810
Bst4CI	ACNGT	4 cut(s)	23, 364, 556, 994
Bst6I	CTCTTC	1 cut(s)	439
BstAPI	GCANNNNNTGC	1 cut(s)	42
BstC8I	GCNNGC	1 cut(s)	75
BstDEI	CTNAG	2 cut(s)	30, 655
BstDSI	CCRYGG	2 cut(s)	6, 810
BstEII	GGTNACC	1 cut(s)	636
BstF5I	GGATG	3 cut(s)	279, 457, 1084
BstFNI	CGCG	1 cut(s)	8
BstHHI	GCGC	1 cut(s)	1059
BstKTI	GATC	6 cut(s)	64, 226, 257, 417, 604, 1102
BstMAI	GTCTC	1 cut(s)	562
BstMBI	GATC	6 cut(s)	61, 223, 254, 414, 601, 1099
BstMCI	CGRYCG	1 cut(s)	12
BstMWI	GCNNNNNNNGC	2 cut(s)	42, 63
BstNSI	RCATGY	1 cut(s)	146
BstPI	GGTNACC	1 cut(s)	636
BstSFI	CTRYAG	2 cut(s)	342, 489
BstSLI	GKGCMC	1 cut(s)	21
BstUI	CGCG	1 cut(s)	8
BstV1I	GCAGC	3 cut(s)	126, 286, 833
BstV2I	GAAGAC	1 cut(s)	603
BstX2I	RGATCY	1 cut(s)	1099
BstYI	RGATCY	1 cut(s)	1099
BsuRI	GGCC	4 cut(s)	5, 669, 706, 936
BtgI	CCRYGG	2 cut(s)	6, 810
BtsCI	GGATG	3 cut(s)	279, 457, 1084
BtsIMutI	CAGTG	2 cut(s)	38, 320
Cac8I	GCNNGC	1 cut(s)	75
CciI	TCATGA	1 cut(s)	220
CfoI	GCGC	1 cut(s)	1059
Cfr13I	GGNCC	2 cut(s)	704, 887
Cfr42I	CCGCGG	1 cut(s)	9
CseI	GACGC	1 cut(s)	934
Csp6I	GTAC	1 cut(s)	266
CviAII	CATG	7 cut(s)	47, 143, 158, 221, 811, 947, 998
CviQI	GTAC	1 cut(s)	266
DdeI	CTNAG	2 cut(s)	30, 655
DpnI	GATC	6 cut(s)	63, 225, 256, 416, 603, 1101
DpnII	GATC	6 cut(s)	61, 223, 254, 414, 601, 1099
DraIII	CACNNNGTG	2 cut(s)	308, 644
EaeI	YGGCCR	2 cut(s)	3, 934
Eam1104I	CTCTTC	1 cut(s)	439
EarI	CTCTTC	1 cut(s)	439
Eco130I	CCWWGG	1 cut(s)	810
Eco24I	GRGCYC	1 cut(s)	59
Eco47I	GGWCC	1 cut(s)	887
Eco57I	CTGAAG	6 cut(s)	134, 549, 578, 867, 1020, 1045
Eco91I	GGTNACC	1 cut(s)	636
EcoO109I	RGGNCCY	1 cut(s)	704
EcoO65I	GGTNACC	1 cut(s)	636
EcoRI	GAATTC	1 cut(s)	229
EcoT14I	CCWWGG	1 cut(s)	810
EcoT38I	GRGCYC	1 cut(s)	59
ErhI	CCWWGG	1 cut(s)	810
FaeI	CATG	7 cut(s)	50, 146, 161, 224, 814, 950, 1001
FalI	AAGNNNNNCTT	4 cut(s)	436, 468, 862, 894
FatI	CATG	7 cut(s)	46, 142, 157, 220, 810, 946, 997
FauNDI	CATATG	2 cut(s)	371, 661
FbaI	TGATCA	1 cut(s)	414
Fnu4HI	GCNGC	5 cut(s)	6, 140, 300, 822, 1025
FokI	GGATG	3 cut(s)	266, 444, 1091
FriOI	GRGCYC	1 cut(s)	59
Fsp4HI	GCNGC	5 cut(s)	6, 140, 300, 822, 1025
FspBI	CTAG	2 cut(s)	248, 779
GlaI	GCGC	1 cut(s)	1058
GluI	GCNGC	5 cut(s)	6, 140, 300, 822, 1025
GsuI	CTGGAG	3 cut(s)	205, 933, 1124
HaeIII	GGCC	4 cut(s)	5, 669, 706, 936
HapII	CCGG	1 cut(s)	286
HgaI	GACGC	1 cut(s)	934
HhaI	GCGC	1 cut(s)	1059
Hin1II	CATG	7 cut(s)	50, 146, 161, 224, 814, 950, 1001
Hin6I	GCGC	1 cut(s)	1057
HinP1I	GCGC	1 cut(s)	1057
HinfI	GANTC	4 cut(s)	319, 431, 545, 1108
HpaII	CCGG	1 cut(s)	286
HphI	GGTGA	1 cut(s)	630
Hpy166II	GTNNAC	4 cut(s)	19, 103, 410, 902
Hpy188I	TCNGA	9 cut(s)	117, 153, 228, 259, 419, 568, 772, 924, 1126
Hpy188III	TCNNGA	6 cut(s)	221, 241, 912, 978, 1071, 1103
Hpy8I	GTNNAC	4 cut(s)	19, 103, 410, 902
Hpy99I	CGWCG	1 cut(s)	536
HpyAV	CCTTC	2 cut(s)	245, 717
HpyCH4III	ACNGT	4 cut(s)	23, 364, 556, 994
HpyF10VI	GCNNNNNNNGC	2 cut(s)	42, 63
HpyF3I	CTNAG	2 cut(s)	30, 655
Hsp92II	CATG	7 cut(s)	50, 146, 161, 224, 814, 950, 1001
HspAI	GCGC	1 cut(s)	1057
KpnI	GGTACC	1 cut(s)	269
Ksp22I	TGATCA	1 cut(s)	414
KspI	CCGCGG	1 cut(s)	9
Kzo9I	GATC	6 cut(s)	61, 223, 254, 414, 601, 1099
LmnI	GCTCC	2 cut(s)	54, 914
Lsp1109I	GCAGC	3 cut(s)	126, 286, 833
LweI	GCATC	6 cut(s)	45, 108, 466, 494, 526, 559
MaeI	CTAG	2 cut(s)	248, 779
MaeIII	GTNAC	3 cut(s)	208, 439, 636
MalI	GATC	6 cut(s)	63, 225, 256, 416, 603, 1101
MboI	GATC	6 cut(s)	61, 223, 254, 414, 601, 1099
MboII	GAAGA	8 cut(s)	140, 426, 464, 555, 584, 603, 873, 882
MflI	RGATCY	1 cut(s)	1099
MhlI	GDGCHC	2 cut(s)	21, 59
MlsI	TGGCCA	1 cut(s)	936
MluCI	AATT	5 cut(s)	165, 229, 588, 873, 1012
MluNI	TGGCCA	1 cut(s)	936
MlyI	GAGTC	2 cut(s)	328, 425
MnlI	CCTC	7 cut(s)	91, 293, 624, 691, 723, 756, 827
Mox20I	TGGCCA	1 cut(s)	936
MroXI	GAANNNNTTC	2 cut(s)	511, 877
MscI	TGGCCA	1 cut(s)	936
MseI	TTAA	3 cut(s)	525, 1011, 1135
MslI	CAYNNNNRTG	1 cut(s)	788
Msp20I	TGGCCA	1 cut(s)	936
MspA1I	CMGCKG	1 cut(s)	8
MspI	CCGG	1 cut(s)	286
Mva1269I	GAATGC	1 cut(s)	731
MvnI	CGCG	1 cut(s)	8
MwoI	GCNNNNNNNGC	2 cut(s)	42, 63
NcoI	CCATGG	1 cut(s)	810
NdeI	CATATG	2 cut(s)	371, 661
NdeII	GATC	6 cut(s)	61, 223, 254, 414, 601, 1099
NlaIII	CATG	7 cut(s)	50, 146, 161, 224, 814, 950, 1001
NlaIV	GGNNCC	5 cut(s)	56, 245, 267, 705, 991
NmuCI	GTSAC	1 cut(s)	636
NspI	RCATGY	1 cut(s)	146
PagI	TCATGA	1 cut(s)	220
PctI	GAATGC	1 cut(s)	731
PdmI	GAANNNNTTC	2 cut(s)	511, 877
PfeI	GAWTC	2 cut(s)	545, 1108
PflMI	CCANNNNNTGG	2 cut(s)	896, 947
PkrI	GCNGC	5 cut(s)	7, 141, 301, 823, 1026
PleI	GAGTC	2 cut(s)	327, 425
PpsI	GAGTC	2 cut(s)	327, 425
PshBI	ATTAAT	1 cut(s)	1011
PsiI	TTATAA	1 cut(s)	851
PspEI	GGTNACC	1 cut(s)	636
PspN4I	GGNNCC	5 cut(s)	56, 245, 267, 705, 991
PspPI	GGNCC	2 cut(s)	704, 887
PsuI	RGATCY	1 cut(s)	1099
RsaI	GTAC	1 cut(s)	267
RsaNI	GTAC	1 cut(s)	266
RseI	CAYNNNNRTG	1 cut(s)	788
SacII	CCGCGG	1 cut(s)	9
SaqAI	TTAA	3 cut(s)	525, 1011, 1135
SatI	GCNGC	5 cut(s)	6, 140, 300, 822, 1025
Sau3AI	GATC	6 cut(s)	61, 223, 254, 414, 601, 1099
Sau96I	GGNCC	2 cut(s)	704, 887
SchI	GAGTC	2 cut(s)	328, 425
SduI	GDGCHC	2 cut(s)	21, 59
SfaNI	GCATC	6 cut(s)	45, 108, 466, 494, 526, 559
SfcI	CTRYAG	2 cut(s)	342, 489
Sfr303I	CCGCGG	1 cut(s)	9
SgrBI	CCGCGG	1 cut(s)	9
SinI	GGWCC	1 cut(s)	887
SmiMI	CAYNNNNRTG	1 cut(s)	788
SpeI	ACTAGT	1 cut(s)	778
Sse9I	AATT	5 cut(s)	165, 229, 588, 873, 1012
SsiI	CCGC	3 cut(s)	6, 8, 1025
SspI	AATATT	2 cut(s)	469, 510
SspMI	CTAG	2 cut(s)	248, 779
StyI	CCWWGG	1 cut(s)	810
TaaI	ACNGT	4 cut(s)	23, 364, 556, 994
TaqI	TCGA	4 cut(s)	12, 534, 548, 693
TasI	AATT	5 cut(s)	165, 229, 588, 873, 1012
TauI	GCSGC	2 cut(s)	8, 1027
TfiI	GAWTC	2 cut(s)	545, 1108
Tru1I	TTAA	3 cut(s)	525, 1011, 1135
Tru9I	TTAA	3 cut(s)	525, 1011, 1135
TscAI	CASTG	2 cut(s)	38, 320
TseFI	GTSAC	1 cut(s)	636
TseI	GCWGC	3 cut(s)	139, 299, 821
Tsp45I	GTSAC	1 cut(s)	636
TspDTI	ATGAA	3 cut(s)	204, 843, 1014
TspGWI	ACGGA	2 cut(s)	725, 740
TspRI	CASTG	2 cut(s)	38, 320
Van91I	CCANNNNNTGG	2 cut(s)	896, 947
VneI	GTGCAC	1 cut(s)	17
VpaK11BI	GGWCC	1 cut(s)	887
VspI	ATTAAT	1 cut(s)	1011
XapI	RAATTY	1 cut(s)	229
XceI	RCATGY	1 cut(s)	146
XcmI	CCANNNNNNNNNTGG	2 cut(s)	170, 944
XmnI	GAANNNNTTC	2 cut(s)	511, 877
XspI	CTAG	2 cut(s)	248, 779

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.