FvH4_6g38290

glucan endo-1,3-beta-glucosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0007207
Physical Location & Seq
Reverse (-)
30282297 .. 30285174
2878 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g38290.t3

Sequence Viewer

Length: 735 bp
ATGATGATTGGTGGAAGGTTTAACTATGGTTTGATAGTATTCCTGATCCTATTGGGTCATTTCTTCTGTTCAGGTCTGAAGGTGAGAATCATAGAACAAAGCCAAGAAGACAAACAGCTACATGTTACTACTTCAATTTCCACAACCCAGAAAGACATCACAACTCCAATAACCACAGTTCCTACAATAACTCCCACCAATCCCACTTCATCAACTCCAATTATGAACCCCACTTCCACACCAGACTCAACCTCACCAGTTACTACAATTCCCTCTTATAGTACCACAACATCTTCTACTTCTGCAAGTTGGTGTGTTGCTAGCCCAAGTGCATCACAAATGGCATTACAAGTTGCCTTGGATTATGCTTGTGGCTATGGTGGCACTGATTGTTCAGAGATTCAGCTTGGTCGAAGTTGTTACAACCCGAACACTGTCCGAGACCATGCTTCTTTTGCATTCAATAACTTTTATCAGAAGAATCCAGTTCCAAATAGCTGCAATTTTGGTGGCACTGCTGTCATCACCAGTACTGATCCAAGTACTGGGACATGTCAATATCCATCCACAAGCACAAGTTCATCGGTTTTGAACACAACAAATTCAAGTGGCTCAACGGTTTTCGGTGCAGTACCTTCTGGTCCTTCTACCTCCTCAGCAGCTGCTGCTGCAAATTTGCATAGCCTGCGAAACTTGTTCACCATGATGTCTCTGATGGTGGTTCTAATCGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
X8

Protein Analysis

245

Amino Acids

25.61

Weight (kDa)

6.38

Isoelectric Point (pI)

43.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 103 - 173 1.6e-20 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 545
AclWI GGATC 2 cut(s) 40, 530
AcsI RAATTY 2 cut(s) 601, 673
AcuI CTGAAG 1 cut(s) 98
AfaI GTAC 4 cut(s) 283, 532, 544, 633
AfiI CCNNNNNNNGG 1 cut(s) 545
AflIII ACRYGT 2 cut(s) 121, 551
AgsI TTSAA 4 cut(s) 135, 463, 592, 606
AloI GAACNNNNNNTCC 2 cut(s) 218, 250
AluBI AGCT 4 cut(s) 118, 406, 498, 662
AluI AGCT 4 cut(s) 118, 406, 498, 662
Alw26I GTCTC 2 cut(s) 435, 714
AlwI GGATC 2 cut(s) 40, 530
AlwNI CAGNNNCTG 2 cut(s) 662, 665
ApeKI GCWGC 5 cut(s) 498, 659, 662, 665, 668
ApoI RAATTY 2 cut(s) 601, 673
AspS9I GGNCC 1 cut(s) 641
AsuHPI GGTGA 4 cut(s) 94, 246, 517, 691
AsuNHI GCTAGC 1 cut(s) 320
AvaII GGWCC 1 cut(s) 641
BbsI GAAGAC 1 cut(s) 114
BbvCI CCTCAGC 1 cut(s) 655
BbvI GCAGC 5 cut(s) 485, 649, 652, 655, 671
BccI CCATC 2 cut(s) 571, 709
BcoDI GTCTC 2 cut(s) 435, 714
BfaI CTAG 1 cut(s) 321
BisI GCNGC 5 cut(s) 499, 660, 663, 666, 669
BlsI GCNGC 5 cut(s) 500, 661, 664, 667, 670
BmcAI AGTACT 2 cut(s) 532, 544
Bme18I GGWCC 1 cut(s) 641
BmgT120I GGNCC 1 cut(s) 641
BmrI ACTGGG 1 cut(s) 555
BmsI GCATC 1 cut(s) 341
BmtI GCTAGC 1 cut(s) 324
BmuI ACTGGG 1 cut(s) 555
BpiI GAAGAC 1 cut(s) 114
Bpu10I CCTNAGC 1 cut(s) 655
BsaI GGTCTC 1 cut(s) 435
BsaJI CCNNGG 1 cut(s) 357
BsaXI ACNNNNNCTCC 2 cut(s) 175, 205
Bsc4I CCNNNNNNNGG 1 cut(s) 545
Bse1I ACTGG 4 cut(s) 257, 485, 528, 550
BseDI CCNNGG 1 cut(s) 357
BseGI GGATG 1 cut(s) 563
BseLI CCNNNNNNNGG 1 cut(s) 545
BseMII CTCAG 1 cut(s) 669
BseNI ACTGG 4 cut(s) 257, 485, 528, 550
BseRI GAGGAG 1 cut(s) 643
BseXI GCAGC 5 cut(s) 485, 649, 652, 655, 671
BsgI GTGCAG 1 cut(s) 648
BslFI GGGAC 1 cut(s) 562
BslI CCNNNNNNNGG 1 cut(s) 545
BsmAI GTCTC 2 cut(s) 435, 714
BsmFI GGGAC 1 cut(s) 562
BsmI GAATGC 1 cut(s) 458
Bso31I GGTCTC 1 cut(s) 435
Bsp143I GATC 2 cut(s) 45, 535
BspCNI CTCAG 1 cut(s) 668
BspOI GCTAGC 1 cut(s) 324
BspPI GGATC 2 cut(s) 40, 530
BspTNI GGTCTC 1 cut(s) 435
BsrI ACTGG 4 cut(s) 257, 485, 528, 550
BssECI CCNNGG 1 cut(s) 357
BssMI GATC 2 cut(s) 45, 535
BssT1I CCWWGG 1 cut(s) 357
Bst4CI ACNGT 3 cut(s) 178, 436, 619
BstAPI GCANNNNNTGC 2 cut(s) 665, 685
BstC8I GCNNGC 2 cut(s) 322, 686
BstDEI CTNAG 1 cut(s) 655
BstF5I GGATG 1 cut(s) 563
BstKTI GATC 2 cut(s) 48, 538
BstMAI GTCTC 2 cut(s) 435, 714
BstMBI GATC 2 cut(s) 45, 535
BstMWI GCNNNNNNNGC 5 cut(s) 381, 455, 665, 668, 685
BstNSI RCATGY 2 cut(s) 125, 555
BstV1I GCAGC 5 cut(s) 485, 649, 652, 655, 671
BstV2I GAAGAC 1 cut(s) 114
BtsCI GGATG 1 cut(s) 563
BtsI GCAGTG 1 cut(s) 513
BtsIMutI CAGTG 3 cut(s) 384, 432, 513
Cac8I GCNNGC 2 cut(s) 322, 686
CaiI CAGNNNCTG 2 cut(s) 662, 665
Cfr13I GGNCC 1 cut(s) 641
Csp6I GTAC 4 cut(s) 282, 531, 543, 632
CspCI CAANNNNNGTGG 2 cut(s) 490, 525
CviAII CATG 4 cut(s) 122, 446, 552, 703
CviJI RGCY 9 cut(s) 102, 118, 324, 375, 406, 498, 612, 662, 684
CviKI_1 RGCY 9 cut(s) 102, 118, 324, 375, 406, 498, 612, 662, 684
CviQI GTAC 4 cut(s) 282, 531, 543, 632
DdeI CTNAG 1 cut(s) 655
DpnI GATC 2 cut(s) 47, 537
DpnII GATC 2 cut(s) 45, 535
Eco130I CCWWGG 1 cut(s) 357
Eco31I GGTCTC 1 cut(s) 435
Eco47I GGWCC 1 cut(s) 641
Eco57I CTGAAG 1 cut(s) 98
EcoT14I CCWWGG 1 cut(s) 357
ErhI CCWWGG 1 cut(s) 357
FaeI CATG 4 cut(s) 125, 449, 555, 706
FaqI GGGAC 1 cut(s) 562
FatI CATG 4 cut(s) 121, 445, 551, 702
Fnu4HI GCNGC 5 cut(s) 499, 660, 663, 666, 669
FokI GGATG 1 cut(s) 550
Fsp4HI GCNGC 5 cut(s) 499, 660, 663, 666, 669
FspBI CTAG 1 cut(s) 321
GluI GCNGC 5 cut(s) 499, 660, 663, 666, 669
Hin1II CATG 4 cut(s) 125, 449, 555, 706
HinfI GANTC 4 cut(s) 87, 245, 400, 481
HphI GGTGA 4 cut(s) 94, 246, 517, 691
Hpy166II GTNNAC 1 cut(s) 699
Hpy188I TCNGA 5 cut(s) 78, 397, 440, 477, 714
Hpy188III TCNNGA 1 cut(s) 43
Hpy8I GTNNAC 1 cut(s) 699
HpyAV CCTTC 4 cut(s) 9, 73, 645, 654
HpyCH4III ACNGT 3 cut(s) 178, 436, 619
HpyCH4V TGCA 7 cut(s) 305, 332, 458, 501, 629, 671, 679
HpyF10VI GCNNNNNNNGC 5 cut(s) 381, 455, 665, 668, 685
HpyF3I CTNAG 1 cut(s) 655
Hsp92II CATG 4 cut(s) 125, 449, 555, 706
Kzo9I GATC 2 cut(s) 45, 535
Lsp1109I GCAGC 5 cut(s) 485, 649, 652, 655, 671
LweI GCATC 1 cut(s) 341
MaeI CTAG 1 cut(s) 321
MaeIII GTNAC 3 cut(s) 124, 259, 419
MalI GATC 2 cut(s) 47, 537
MboI GATC 2 cut(s) 45, 535
MboII GAAGA 4 cut(s) 55, 119, 285, 490
MluCI AATT 6 cut(s) 135, 219, 267, 502, 601, 673
MlyI GAGTC 1 cut(s) 239
MnlI CCTC 4 cut(s) 262, 283, 661, 664
MseI TTAA 2 cut(s) 21, 733
MslI CAYNNNNRTG 1 cut(s) 704
MspA1I CMGCKG 1 cut(s) 662
Mva1269I GAATGC 1 cut(s) 458
MwoI GCNNNNNNNGC 5 cut(s) 381, 455, 665, 668, 685
NdeII GATC 2 cut(s) 45, 535
NheI GCTAGC 1 cut(s) 320
NlaIII CATG 4 cut(s) 125, 449, 555, 706
NspI RCATGY 2 cut(s) 125, 555
PciI ACATGT 2 cut(s) 121, 551
PctI GAATGC 1 cut(s) 458
PfeI GAWTC 3 cut(s) 87, 400, 481
PflMI CCANNNNNTGG 1 cut(s) 545
PkrI GCNGC 5 cut(s) 500, 661, 664, 667, 670
PleI GAGTC 1 cut(s) 239
PpsI GAGTC 1 cut(s) 239
PscI ACATGT 2 cut(s) 121, 551
PspPI GGNCC 1 cut(s) 641
PstNI CAGNNNCTG 2 cut(s) 662, 665
PvuII CAGCTG 1 cut(s) 662
RsaI GTAC 4 cut(s) 283, 532, 544, 633
RsaNI GTAC 4 cut(s) 282, 531, 543, 632
RseI CAYNNNNRTG 1 cut(s) 704
SaqAI TTAA 2 cut(s) 21, 733
SatI GCNGC 5 cut(s) 499, 660, 663, 666, 669
Sau3AI GATC 2 cut(s) 45, 535
Sau96I GGNCC 1 cut(s) 641
ScaI AGTACT 2 cut(s) 532, 544
SchI GAGTC 1 cut(s) 239
SfaNI GCATC 1 cut(s) 341
SinI GGWCC 1 cut(s) 641
SmiMI CAYNNNNRTG 1 cut(s) 704
Sse9I AATT 6 cut(s) 135, 219, 267, 502, 601, 673
SspMI CTAG 1 cut(s) 321
StyI CCWWGG 1 cut(s) 357
TaaI ACNGT 3 cut(s) 178, 436, 619
TaqI TCGA 1 cut(s) 412
TasI AATT 6 cut(s) 135, 219, 267, 502, 601, 673
TatI WGTACW 2 cut(s) 530, 542
TfiI GAWTC 3 cut(s) 87, 400, 481
Tru1I TTAA 2 cut(s) 21, 733
Tru9I TTAA 2 cut(s) 21, 733
TscAI CASTG 3 cut(s) 391, 439, 520
TseI GCWGC 5 cut(s) 498, 659, 662, 665, 668
TspDTI ATGAA 3 cut(s) 198, 239, 570
TspRI CASTG 3 cut(s) 391, 439, 520
Van91I CCANNNNNTGG 1 cut(s) 545
VpaK11BI GGWCC 1 cut(s) 641
XapI RAATTY 2 cut(s) 601, 673
XceI RCATGY 2 cut(s) 125, 555
XspI CTAG 1 cut(s) 321
ZrmI AGTACT 2 cut(s) 532, 544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.