MD09G1151300.v1.1

glucan endo-1,3-beta-glucosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0007207
Physical Location & Seq
Forward (+)
11938015 .. 11942197
4183 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1151300.v1.1.491

Sequence Viewer

Length: 786 bp
ATGGGTGGAAGGTTTATTTATCCTATAACTCTAATCTCCTGCTTGTTACTCCTATTGGGTATTTTCTTCTGTTCAGGTTTGAGTGTGACAATAAAGGAAAAGAACATTGGACACATCAAAGAAAAAGACCAAGAAAATAAAGGGCTGAAATTCCCTGAAACCCTAATTTCCACCACCCAAAGGGACATCACTACTCCAATAACAACAGTTCCTACAATAACTACCACAAATCCCACTTCATCAACACCAATTGTGAACCCTAATTCAAATCCAGCTGATTCAACTGTCCCTGTCACATCCTCCCCTAGCATGACCCCATTTCCTACAACAACAACAACACCATCATCACCTGCATCCACTGGTTCAAGTTGGTGTGTTGCTAGCCAAGGTGCATCACAAATGGCATTGCAAGTTGCTTTGGACTATGCTTGTGGCCATGGTGGCACTGATTGCTCAGAAATTCAGCCGGGACGAAGCTGTTACAACCCAAATAGTGTCCGGGATCATGCTTCTTACGCATTCAATAACTATTATCAGAAGAAACCAGTTCCAAATAGCTGCAACTTTGGTGGAACTGCTATGATCACTAGCACTGACCCAAGTACAGGGTCTTGTCAATATCCGTCTACTAGCACAAGTTCATCAGTCTTGAACACAACAAATACTAGTGGCTCAACTGTGTTTGGTGCGGTCCCGTCAGGCCCTACCACCTCAGCAGCAACAGTAGATGCAAACACACCCAGCCTGCAAAATATTTTGTACATTATGGCATGTTTGATGGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
X8

Protein Analysis

262

Amino Acids

27.31

Weight (kDa)

6.25

Isoelectric Point (pI)

51.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 123 - 194 2.5e-21 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 358
Acc36I ACCTGC 1 cut(s) 358
AccI GTMKAC 1 cut(s) 626
AciI CCGC 1 cut(s) 689
AclWI GGATC 1 cut(s) 510
AcoI YGGCCR 1 cut(s) 433
AcsI RAATTY 2 cut(s) 149, 459
AfaI GTAC 2 cut(s) 604, 761
AfiI CCNNNNNNNGG 2 cut(s) 180, 605
AgsI TTSAA 5 cut(s) 267, 282, 366, 523, 652
AhlI ACTAGT 1 cut(s) 665
AjuI GAANNNNNNNTTGG 2 cut(s) 90, 122
AluBI AGCT 3 cut(s) 275, 477, 558
AluI AGCT 3 cut(s) 275, 477, 558
AlwI GGATC 1 cut(s) 510
AoxI GGCC 2 cut(s) 433, 700
ApeKI GCWGC 2 cut(s) 558, 716
ApoI RAATTY 2 cut(s) 149, 459
AspS9I GGNCC 2 cut(s) 691, 701
AsuC2I CCSGG 2 cut(s) 468, 500
AsuHPI GGTGA 1 cut(s) 339
AsuNHI GCTAGC 1 cut(s) 380
AvaII GGWCC 1 cut(s) 691
BalI TGGCCA 1 cut(s) 435
BbvCI CCTCAGC 1 cut(s) 712
BbvI GCAGC 2 cut(s) 545, 728
BccI CCATC 2 cut(s) 349, 772
BclI TGATCA 1 cut(s) 582
BcnI CCSGG 2 cut(s) 468, 500
BcuI ACTAGT 1 cut(s) 665
BfaI CTAG 5 cut(s) 306, 381, 588, 630, 666
BfuAI ACCTGC 1 cut(s) 358
BglI GCCNNNNNGGC 1 cut(s) 441
BisI GCNGC 2 cut(s) 559, 717
BlsI GCNGC 2 cut(s) 560, 718
Bme1390I CCNGG 2 cut(s) 468, 500
Bme18I GGWCC 1 cut(s) 691
BmgT120I GGNCC 2 cut(s) 691, 701
BmiI GGNNCC 1 cut(s) 693
BmrFI CCNGG 2 cut(s) 468, 500
BmsI GCATC 3 cut(s) 362, 401, 718
BmtI GCTAGC 1 cut(s) 384
Bpu10I CCTNAGC 1 cut(s) 712
BpuMI CCSGG 2 cut(s) 468, 500
BsaJI CCNNGG 2 cut(s) 385, 436
Bsc4I CCNNNNNNNGG 2 cut(s) 180, 605
Bse1I ACTGG 2 cut(s) 364, 545
Bse3DI GCAATG 1 cut(s) 404
BseDI CCNNGG 2 cut(s) 385, 436
BseGI GGATG 2 cut(s) 296, 353
BseLI CCNNNNNNNGG 2 cut(s) 180, 605
BseMI GCAATG 1 cut(s) 404
BseMII CTCAG 2 cut(s) 468, 726
BseNI ACTGG 2 cut(s) 364, 545
BseXI GCAGC 2 cut(s) 545, 728
BseYI CCCAGC 1 cut(s) 740
BshFI GGCC 2 cut(s) 435, 702
BsiSI CCGG 2 cut(s) 467, 499
BslFI GGGAC 4 cut(s) 197, 272, 483, 677
BslI CCNNNNNNNGG 2 cut(s) 180, 605
BsmFI GGGAC 4 cut(s) 197, 272, 483, 677
BsmI GAATGC 1 cut(s) 518
BsnI GGCC 2 cut(s) 435, 702
Bsp1407I TGTACA 1 cut(s) 759
Bsp143I GATC 2 cut(s) 502, 582
Bsp19I CCATGG 1 cut(s) 436
BspACI CCGC 1 cut(s) 689
BspANI GGCC 2 cut(s) 435, 702
BspCNI CTCAG 2 cut(s) 467, 725
BspLI GGNNCC 1 cut(s) 693
BspMI ACCTGC 1 cut(s) 358
BspOI GCTAGC 1 cut(s) 384
BspPI GGATC 1 cut(s) 510
BsrDI GCAATG 1 cut(s) 404
BsrGI TGTACA 1 cut(s) 759
BsrI ACTGG 2 cut(s) 364, 545
BssECI CCNNGG 2 cut(s) 385, 436
BssMI GATC 2 cut(s) 502, 582
BssT1I CCWWGG 2 cut(s) 385, 436
Bst4CI ACNGT 4 cut(s) 208, 286, 679, 724
BstAPI GCANNNNNTGC 1 cut(s) 450
BstAUI TGTACA 1 cut(s) 759
BstC8I GCNNGC 2 cut(s) 382, 746
BstDEI CTNAG 2 cut(s) 454, 712
BstDSI CCRYGG 1 cut(s) 436
BstF5I GGATG 2 cut(s) 296, 353
BstKTI GATC 2 cut(s) 505, 585
BstMBI GATC 2 cut(s) 502, 582
BstMWI GCNNNNNNNGC 3 cut(s) 441, 450, 515
BstNSI RCATGY 1 cut(s) 774
BstSCI CCNGG 2 cut(s) 466, 498
BstV1I GCAGC 2 cut(s) 545, 728
BsuRI GGCC 2 cut(s) 435, 702
BtgI CCRYGG 1 cut(s) 436
BtsCI GGATG 2 cut(s) 296, 353
BtsIMutI CAGTG 3 cut(s) 357, 444, 591
BveI ACCTGC 1 cut(s) 358
Cac8I GCNNGC 2 cut(s) 382, 746
Cfr13I GGNCC 2 cut(s) 691, 701
Csp6I GTAC 2 cut(s) 603, 760
CspCI CAANNNNNGTGG 2 cut(s) 550, 585
CviAII CATG 4 cut(s) 310, 437, 506, 771
CviQI GTAC 2 cut(s) 603, 760
DdeI CTNAG 2 cut(s) 454, 712
DpnI GATC 2 cut(s) 504, 584
DpnII GATC 2 cut(s) 502, 582
EaeI YGGCCR 1 cut(s) 433
Eco130I CCWWGG 2 cut(s) 385, 436
Eco47I GGWCC 1 cut(s) 691
EcoO109I RGGNCCY 1 cut(s) 701
EcoT14I CCWWGG 2 cut(s) 385, 436
ErhI CCWWGG 2 cut(s) 385, 436
FaeI CATG 4 cut(s) 313, 440, 509, 774
FaiI YATR 8 cut(s) 26, 311, 426, 438, 507, 581, 767, 772
FaqI GGGAC 4 cut(s) 197, 272, 483, 677
FatI CATG 4 cut(s) 309, 436, 505, 770
FbaI TGATCA 1 cut(s) 582
FblI GTMKAC 1 cut(s) 626
Fnu4HI GCNGC 2 cut(s) 559, 717
FokI GGATG 2 cut(s) 283, 340
Fsp4HI GCNGC 2 cut(s) 559, 717
FspBI CTAG 5 cut(s) 306, 381, 588, 630, 666
GluI GCNGC 2 cut(s) 559, 717
GsaI CCCAGC 1 cut(s) 744
HaeIII GGCC 2 cut(s) 435, 702
HapII CCGG 2 cut(s) 467, 499
Hin1II CATG 4 cut(s) 313, 440, 509, 774
HinfI GANTC 1 cut(s) 278
HpaII CCGG 2 cut(s) 467, 499
HphI GGTGA 1 cut(s) 339
Hpy166II GTNNAC 2 cut(s) 256, 627
Hpy188I TCNGA 2 cut(s) 457, 537
Hpy188III TCNNGA 1 cut(s) 649
Hpy8I GTNNAC 2 cut(s) 256, 627
HpyAV CCTTC 1 cut(s) 3
HpyCH4III ACNGT 4 cut(s) 208, 286, 679, 724
HpyCH4V TGCA 6 cut(s) 353, 392, 409, 561, 731, 748
HpyF10VI GCNNNNNNNGC 3 cut(s) 441, 450, 515
HpyF3I CTNAG 2 cut(s) 454, 712
Hsp92II CATG 4 cut(s) 313, 440, 509, 774
Ksp22I TGATCA 1 cut(s) 582
Kzo9I GATC 2 cut(s) 502, 582
Lsp1109I GCAGC 2 cut(s) 545, 728
LweI GCATC 3 cut(s) 362, 401, 718
MaeI CTAG 5 cut(s) 306, 381, 588, 630, 666
MaeIII GTNAC 4 cut(s) 45, 85, 292, 479
MalI GATC 2 cut(s) 504, 584
MboI GATC 2 cut(s) 502, 582
MboII GAAGA 2 cut(s) 58, 550
MfeI CAATTG 1 cut(s) 249
MlsI TGGCCA 1 cut(s) 435
MluCI AATT 5 cut(s) 149, 165, 249, 262, 459
MluNI TGGCCA 1 cut(s) 435
MnlI CCTC 2 cut(s) 310, 721
Mox20I TGGCCA 1 cut(s) 435
MscI TGGCCA 1 cut(s) 435
Msp20I TGGCCA 1 cut(s) 435
MspA1I CMGCKG 1 cut(s) 275
MspI CCGG 2 cut(s) 467, 499
MspR9I CCNGG 2 cut(s) 468, 500
MunI CAATTG 1 cut(s) 249
Mva1269I GAATGC 1 cut(s) 518
MwoI GCNNNNNNNGC 3 cut(s) 441, 450, 515
NciI CCSGG 2 cut(s) 468, 500
NcoI CCATGG 1 cut(s) 436
NdeII GATC 2 cut(s) 502, 582
NheI GCTAGC 1 cut(s) 380
NlaIII CATG 4 cut(s) 313, 440, 509, 774
NlaIV GGNNCC 1 cut(s) 693
NmuCI GTSAC 2 cut(s) 85, 292
NspI RCATGY 1 cut(s) 774
PaqCI CACCTGC 1 cut(s) 358
PctI GAATGC 1 cut(s) 518
PfeI GAWTC 1 cut(s) 278
PfoI TCCNGGA 1 cut(s) 498
PkrI GCNGC 2 cut(s) 560, 718
PspFI CCCAGC 1 cut(s) 740
PspN4I GGNNCC 1 cut(s) 693
PspPI GGNCC 2 cut(s) 691, 701
PvuII CAGCTG 1 cut(s) 275
RsaI GTAC 2 cut(s) 604, 761
RsaNI GTAC 2 cut(s) 603, 760
SatI GCNGC 2 cut(s) 559, 717
Sau3AI GATC 2 cut(s) 502, 582
Sau96I GGNCC 2 cut(s) 691, 701
ScrFI CCNGG 2 cut(s) 468, 500
SetI ASST 8 cut(s) 14, 79, 277, 352, 391, 479, 560, 713
SfaNI GCATC 3 cut(s) 362, 401, 718
SinI GGWCC 1 cut(s) 691
SpeI ACTAGT 1 cut(s) 665
Sse9I AATT 5 cut(s) 149, 165, 249, 262, 459
SsiI CCGC 1 cut(s) 689
SspI AATATT 1 cut(s) 754
SspMI CTAG 5 cut(s) 306, 381, 588, 630, 666
StyD4I CCNGG 2 cut(s) 466, 498
StyI CCWWGG 2 cut(s) 385, 436
TaaI ACNGT 4 cut(s) 208, 286, 679, 724
TasI AATT 5 cut(s) 149, 165, 249, 262, 459
TatI WGTACW 2 cut(s) 602, 759
TfiI GAWTC 1 cut(s) 278
TscAI CASTG 3 cut(s) 364, 451, 598
TseFI GTSAC 2 cut(s) 85, 292
TseI GCWGC 2 cut(s) 558, 716
Tsp45I GTSAC 2 cut(s) 85, 292
TspDTI ATGAA 2 cut(s) 228, 630
TspGWI ACGGA 1 cut(s) 612
TspRI CASTG 3 cut(s) 364, 451, 598
VpaK11BI GGWCC 1 cut(s) 691
XapI RAATTY 2 cut(s) 149, 459
XceI RCATGY 1 cut(s) 774
XmiI GTMKAC 1 cut(s) 626
XspI CTAG 5 cut(s) 306, 381, 588, 630, 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.