RLG00000020562

glucan endo-1,3-beta-glucosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0007207
Physical Location & Seq
Reverse (-)
66302162 .. 66304156
1995 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020562

Sequence Viewer

Length: 735 bp
ATGATGATTGGTGGAAGGTTCAACTATGGTATAATAGTATTCCTGATCCTATTGGGTCATTTCTTCTGTTCAGGTTTGAAGGTGAGAATTAAAGAACAAAGCCATGAACAGAAACAGCTACATATTACTACTTCAATTTCCACAACCCAGAAAGACATCACAACCCCAATAACCACAGTTCCTACAATAACTCCCACCACTCCCACTTCATCAACACCAATTATGAACCCTACTTCCACACCAGATTCAACCTCTCCAGTTACTACAACCCCCTTTTCTACTACCACAACATCATCCACTTCTGCAAGTTGGTGTGTTGCTAGCCCAAGTGCATCACAAATGGCATTACAAGTTGCCTTGGACTATGCTTGTGGCTATGGTGGCACTGATTGTTCAGAGATTCAGCTTGGTCGAAGTTGTTACAGCCCAAATACTGTCCGAGACCATGCTTCATTTGCATTCAATAACTTTTATCAGAAGAATCCAGTTCCAAATAGCTGCAATTTTGGCGGCACTGCTGTCATCACCAACAATGATCCAAGTACTGGGACATGTCAATATCCATCCACTAGCACAAGTTCATCGGTTTTGAACACAACAAATTCAAGTGGCTCAACGGTTTTCGGTGCAGTACCTTCCGGTCCTTCTACCTCTTCAGCAGCTGCTGCTGCAAGTTCGCATAGCCTGCGAACCTTGTTCACTATGATGTCTCTGACGGTGGTTCTACTCGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
X8

Protein Analysis

245

Amino Acids

25.55

Weight (kDa)

7.61

Isoelectric Point (pI)

43.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 103 - 173 1.4e-20 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 545
AciI CCGC 1 cut(s) 510
AclWI GGATC 2 cut(s) 40, 530
AcsI RAATTY 1 cut(s) 601
AcuI CTGAAG 1 cut(s) 639
AfaI GTAC 2 cut(s) 544, 633
AfiI CCNNNNNNNGG 1 cut(s) 545
AflIII ACRYGT 1 cut(s) 551
AgsI TTSAA 7 cut(s) 22, 79, 135, 249, 463, 592, 606
AloI GAACNNNNNNTCC 2 cut(s) 218, 250
AluBI AGCT 4 cut(s) 118, 406, 498, 662
AluI AGCT 4 cut(s) 118, 406, 498, 662
Alw26I GTCTC 2 cut(s) 435, 714
AlwI GGATC 2 cut(s) 40, 530
AlwNI CAGNNNCTG 2 cut(s) 662, 665
ApeKI GCWGC 5 cut(s) 498, 659, 662, 665, 668
ApoI RAATTY 1 cut(s) 601
AspS9I GGNCC 1 cut(s) 641
AsuHPI GGTGA 2 cut(s) 94, 517
AsuNHI GCTAGC 1 cut(s) 320
AvaII GGWCC 1 cut(s) 641
BbvI GCAGC 5 cut(s) 485, 649, 652, 655, 671
BccI CCATC 1 cut(s) 571
BcoDI GTCTC 2 cut(s) 435, 714
BfaI CTAG 2 cut(s) 321, 570
BisI GCNGC 6 cut(s) 499, 511, 660, 663, 666, 669
BlsI GCNGC 6 cut(s) 500, 512, 661, 664, 667, 670
BmcAI AGTACT 1 cut(s) 544
Bme18I GGWCC 1 cut(s) 641
BmgT120I GGNCC 1 cut(s) 641
BmrI ACTGGG 1 cut(s) 555
BmsI GCATC 1 cut(s) 341
BmtI GCTAGC 1 cut(s) 324
BmuI ACTGGG 1 cut(s) 555
BpmI CTGGAG 1 cut(s) 240
BsaI GGTCTC 1 cut(s) 435
BsaJI CCNNGG 1 cut(s) 357
BsaWI WCCGGW 1 cut(s) 638
BsaXI ACNNNNNCTCC 2 cut(s) 175, 205
Bsc4I CCNNNNNNNGG 1 cut(s) 545
Bse1I ACTGG 3 cut(s) 257, 485, 550
BseDI CCNNGG 1 cut(s) 357
BseGI GGATG 2 cut(s) 293, 563
BseLI CCNNNNNNNGG 1 cut(s) 545
BseNI ACTGG 3 cut(s) 257, 485, 550
BseXI GCAGC 5 cut(s) 485, 649, 652, 655, 671
BsgI GTGCAG 1 cut(s) 648
BsiSI CCGG 1 cut(s) 639
BslFI GGGAC 1 cut(s) 562
BslI CCNNNNNNNGG 1 cut(s) 545
BsmAI GTCTC 2 cut(s) 435, 714
BsmFI GGGAC 1 cut(s) 562
BsmI GAATGC 1 cut(s) 458
Bso31I GGTCTC 1 cut(s) 435
Bsp143I GATC 2 cut(s) 45, 535
BspACI CCGC 1 cut(s) 510
BspOI GCTAGC 1 cut(s) 324
BspPI GGATC 2 cut(s) 40, 530
BspTNI GGTCTC 1 cut(s) 435
BsrI ACTGG 3 cut(s) 257, 485, 550
BssECI CCNNGG 1 cut(s) 357
BssMI GATC 2 cut(s) 45, 535
BssT1I CCWWGG 1 cut(s) 357
Bst4CI ACNGT 4 cut(s) 178, 436, 619, 718
Bst6I CTCTTC 1 cut(s) 658
BstAPI GCANNNNNTGC 2 cut(s) 665, 685
BstC8I GCNNGC 2 cut(s) 322, 686
BstF5I GGATG 2 cut(s) 293, 563
BstKTI GATC 2 cut(s) 48, 538
BstMAI GTCTC 2 cut(s) 435, 714
BstMBI GATC 2 cut(s) 45, 535
BstMWI GCNNNNNNNGC 6 cut(s) 381, 455, 507, 665, 668, 685
BstNSI RCATGY 1 cut(s) 555
BstV1I GCAGC 5 cut(s) 485, 649, 652, 655, 671
BtsCI GGATG 2 cut(s) 293, 563
BtsI GCAGTG 1 cut(s) 513
BtsIMutI CAGTG 2 cut(s) 384, 513
Cac8I GCNNGC 2 cut(s) 322, 686
CaiI CAGNNNCTG 2 cut(s) 662, 665
Cfr13I GGNCC 1 cut(s) 641
Csp6I GTAC 2 cut(s) 543, 632
CviAII CATG 3 cut(s) 104, 446, 552
CviQI GTAC 2 cut(s) 543, 632
DpnI GATC 2 cut(s) 47, 537
DpnII GATC 2 cut(s) 45, 535
Eam1104I CTCTTC 1 cut(s) 658
EarI CTCTTC 1 cut(s) 658
Eco130I CCWWGG 1 cut(s) 357
Eco31I GGTCTC 1 cut(s) 435
Eco47I GGWCC 1 cut(s) 641
Eco57I CTGAAG 1 cut(s) 639
EcoT14I CCWWGG 1 cut(s) 357
ErhI CCWWGG 1 cut(s) 357
FaeI CATG 3 cut(s) 107, 449, 555
FaqI GGGAC 1 cut(s) 562
FatI CATG 3 cut(s) 103, 445, 551
Fnu4HI GCNGC 6 cut(s) 499, 511, 660, 663, 666, 669
FokI GGATG 2 cut(s) 280, 550
Fsp4HI GCNGC 6 cut(s) 499, 511, 660, 663, 666, 669
FspBI CTAG 2 cut(s) 321, 570
GluI GCNGC 6 cut(s) 499, 511, 660, 663, 666, 669
GsuI CTGGAG 1 cut(s) 240
HapII CCGG 1 cut(s) 639
Hin1II CATG 3 cut(s) 107, 449, 555
HinfI GANTC 3 cut(s) 245, 400, 481
HpaII CCGG 1 cut(s) 639
HphI GGTGA 2 cut(s) 94, 517
Hpy166II GTNNAC 1 cut(s) 699
Hpy188I TCNGA 4 cut(s) 397, 440, 477, 714
Hpy188III TCNNGA 1 cut(s) 43
Hpy8I GTNNAC 1 cut(s) 699
HpyAV CCTTC 4 cut(s) 9, 73, 645, 654
HpyCH4III ACNGT 4 cut(s) 178, 436, 619, 718
HpyCH4V TGCA 6 cut(s) 305, 332, 458, 501, 629, 671
HpyF10VI GCNNNNNNNGC 6 cut(s) 381, 455, 507, 665, 668, 685
Hsp92II CATG 3 cut(s) 107, 449, 555
Kzo9I GATC 2 cut(s) 45, 535
LpnPI CCDG 9 cut(s) 56, 57, 161, 255, 270, 498, 531, 652, 698
Lsp1109I GCAGC 5 cut(s) 485, 649, 652, 655, 671
LweI GCATC 1 cut(s) 341
MaeI CTAG 2 cut(s) 321, 570
MaeIII GTNAC 2 cut(s) 259, 419
MalI GATC 2 cut(s) 47, 537
MboI GATC 2 cut(s) 45, 535
MboII GAAGA 3 cut(s) 55, 490, 645
MluCI AATT 5 cut(s) 87, 135, 219, 502, 601
MnlI CCTC 2 cut(s) 262, 661
MseI TTAA 2 cut(s) 90, 733
MslI CAYNNNNRTG 1 cut(s) 704
MspA1I CMGCKG 1 cut(s) 662
MspI CCGG 1 cut(s) 639
Mva1269I GAATGC 1 cut(s) 458
MwoI GCNNNNNNNGC 6 cut(s) 381, 455, 507, 665, 668, 685
NdeII GATC 2 cut(s) 45, 535
NheI GCTAGC 1 cut(s) 320
NlaIII CATG 3 cut(s) 107, 449, 555
NspI RCATGY 1 cut(s) 555
PciI ACATGT 1 cut(s) 551
PctI GAATGC 1 cut(s) 458
PfeI GAWTC 3 cut(s) 245, 400, 481
PflMI CCANNNNNTGG 1 cut(s) 545
PkrI GCNGC 6 cut(s) 500, 512, 661, 664, 667, 670
PscI ACATGT 1 cut(s) 551
PspPI GGNCC 1 cut(s) 641
PstNI CAGNNNCTG 2 cut(s) 662, 665
PvuII CAGCTG 1 cut(s) 662
RsaI GTAC 2 cut(s) 544, 633
RsaNI GTAC 2 cut(s) 543, 632
RseI CAYNNNNRTG 1 cut(s) 704
SaqAI TTAA 2 cut(s) 90, 733
SatI GCNGC 6 cut(s) 499, 511, 660, 663, 666, 669
Sau3AI GATC 2 cut(s) 45, 535
Sau96I GGNCC 1 cut(s) 641
ScaI AGTACT 1 cut(s) 544
SfaNI GCATC 1 cut(s) 341
SinI GGWCC 1 cut(s) 641
SmiMI CAYNNNNRTG 1 cut(s) 704
Sse9I AATT 5 cut(s) 87, 135, 219, 502, 601
SsiI CCGC 1 cut(s) 510
SspMI CTAG 2 cut(s) 321, 570
StyI CCWWGG 1 cut(s) 357
TaaI ACNGT 4 cut(s) 178, 436, 619, 718
TaqI TCGA 1 cut(s) 412
TasI AATT 5 cut(s) 87, 135, 219, 502, 601
TatI WGTACW 1 cut(s) 542
TauI GCSGC 1 cut(s) 513
TfiI GAWTC 3 cut(s) 245, 400, 481
Tru1I TTAA 2 cut(s) 90, 733
Tru9I TTAA 2 cut(s) 90, 733
TscAI CASTG 2 cut(s) 391, 520
TseI GCWGC 5 cut(s) 498, 659, 662, 665, 668
TspDTI ATGAA 5 cut(s) 120, 198, 239, 441, 570
TspRI CASTG 2 cut(s) 391, 520
Van91I CCANNNNNTGG 1 cut(s) 545
VpaK11BI GGWCC 1 cut(s) 641
XapI RAATTY 1 cut(s) 601
XceI RCATGY 1 cut(s) 555
XspI CTAG 2 cut(s) 321, 570
ZrmI AGTACT 1 cut(s) 544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.