MD17G1139000.v1.1

glucan endo-1,3-beta-glucosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0007207
Physical Location & Seq
Forward (+)
12457064 .. 12459827
2764 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1139000.v1.1.491

Sequence Viewer

Length: 789 bp
ATGGGTGGAAGGTTTATTTATCCTGTAATTCTAGGCTCCTGCTTGTTCCTCCTGTTGGGTAATTTCTTCTGTTCAGGTTTGAAGGTGACAAAAAAAGAAAAGAACATTGGACACAGCAAAGAACAAGACCAAGAAAATAAAGGGATGAAATTCCCTAAAACCCTAATTTCCACCACCCAAAGAGACATCACTACTCCAATAACAACAGTCCCTACAATAAATCCCACAAATCCCACTTCATCAACACCAATTGTGAACCCTAATTCCAATCCAGCTGATTCAACATTTCCTGTCACAACAACGCCTAGCATGACCCCATATACTGCAACAACACCATCACCACCTGCATCCACTGGTTCAAGTTGGTGTGTTTCTAGCCAAAGTGCATCACAAATGGCATTACAAGTTGCTTTGGACTATGCTTGTGGCTATGGTGGCACTGATTGCTCAGAGATTCAGCCAGGACGAAGCTGTTATAATCCAAACAATGTCCGGGATCACGCTTCTTTCGCATTCAATAACTATTATCAGAAGAACCCAGTTCCAAATAGCTGCAACTTTGGTGGAACTGCTGTGATCACTAGCACTGACCCAAGTACTGGGACGTGTCAATATCCATCCACTAGCACAAGTTCATCAGTCTTGAACACAACAAATACGAGTGGCTCCACTGTGTTTGGTGCTGTCCCTTCTGGCCCTACTACCTCAGCAGCAATAGTAGCTGCAAACTCCTCACATGGCGTGCACAATTTGTTCATCGTGGCATGTTTTCTGGTGTTAACAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
X8

Protein Analysis

263

Amino Acids

27.48

Weight (kDa)

8.06

Isoelectric Point (pI)

44.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 121 - 191 2.2e-20 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 477
AarI CACCTGC 1 cut(s) 352
Acc36I ACCTGC 1 cut(s) 352
AccB7I CCANNNNNTGG 1 cut(s) 599
AclWI GGATC 1 cut(s) 504
AcsI RAATTY 1 cut(s) 149
AfaI GTAC 1 cut(s) 598
AfiI CCNNNNNNNGG 2 cut(s) 55, 599
AflIII ACRYGT 1 cut(s) 605
AgsI TTSAA 5 cut(s) 82, 282, 360, 517, 646
AjiI CACGTC 1 cut(s) 606
AjnI CCWGG 1 cut(s) 460
AjuI GAANNNNNNNTTGG 2 cut(s) 90, 122
AloI GAACNNNNNNTCC 2 cut(s) 248, 280
AluBI AGCT 4 cut(s) 275, 471, 552, 722
AluI AGCT 4 cut(s) 275, 471, 552, 722
Alw21I GWGCWC 1 cut(s) 747
Alw26I GTCTC 1 cut(s) 177
Alw44I GTGCAC 1 cut(s) 743
AlwI GGATC 1 cut(s) 504
AoxI GGCC 1 cut(s) 694
ApaLI GTGCAC 1 cut(s) 743
ApeKI GCWGC 3 cut(s) 552, 710, 722
ApoI RAATTY 1 cut(s) 149
AspS9I GGNCC 1 cut(s) 695
AsuC2I CCSGG 1 cut(s) 494
AsuHPI GGTGA 2 cut(s) 97, 330
BaeGI GKGCMC 1 cut(s) 747
Bbv12I GWGCWC 1 cut(s) 747
BbvCI CCTCAGC 1 cut(s) 706
BbvI GCAGC 3 cut(s) 539, 709, 722
BccI CCATC 2 cut(s) 343, 625
BciT130I CCWGG 1 cut(s) 462
BclI TGATCA 1 cut(s) 576
BcnI CCSGG 1 cut(s) 494
BcoDI GTCTC 1 cut(s) 177
BfaI CTAG 5 cut(s) 32, 306, 375, 582, 624
BfuAI ACCTGC 1 cut(s) 352
BisI GCNGC 3 cut(s) 553, 711, 723
BlsI GCNGC 3 cut(s) 554, 712, 724
BmcAI AGTACT 1 cut(s) 598
Bme1390I CCNGG 2 cut(s) 462, 494
BmgBI CACGTC 1 cut(s) 606
BmgT120I GGNCC 1 cut(s) 695
BmiI GGNNCC 2 cut(s) 37, 667
BmrFI CCNGG 2 cut(s) 462, 494
BmrI ACTGGG 2 cut(s) 533, 609
BmsI GCATC 2 cut(s) 356, 395
BmuI ACTGGG 2 cut(s) 533, 609
Bpu10I CCTNAGC 1 cut(s) 706
BpuMI CCSGG 1 cut(s) 494
Bsc4I CCNNNNNNNGG 2 cut(s) 55, 599
Bse1I ACTGG 3 cut(s) 358, 539, 604
BseBI CCWGG 1 cut(s) 462
BseGI GGATG 3 cut(s) 150, 347, 617
BseLI CCNNNNNNNGG 2 cut(s) 55, 599
BseMII CTCAG 2 cut(s) 462, 720
BseNI ACTGG 3 cut(s) 358, 539, 604
BseRI GAGGAG 1 cut(s) 721
BseSI GKGCMC 1 cut(s) 747
BseXI GCAGC 3 cut(s) 539, 709, 722
BshFI GGCC 1 cut(s) 696
BsiHKAI GWGCWC 1 cut(s) 747
BsiSI CCGG 1 cut(s) 493
BslFI GGGAC 3 cut(s) 194, 616, 671
BslI CCNNNNNNNGG 2 cut(s) 55, 599
BsmAI GTCTC 1 cut(s) 177
BsmFI GGGAC 3 cut(s) 194, 616, 671
BsmI GAATGC 1 cut(s) 512
BsnI GGCC 1 cut(s) 696
Bsp1286I GDGCHC 1 cut(s) 747
Bsp143I GATC 2 cut(s) 496, 576
BspANI GGCC 1 cut(s) 696
BspCNI CTCAG 2 cut(s) 461, 719
BspLI GGNNCC 2 cut(s) 37, 667
BspMI ACCTGC 1 cut(s) 352
BspPI GGATC 1 cut(s) 504
BsrI ACTGG 3 cut(s) 358, 539, 604
BssMI GATC 2 cut(s) 496, 576
Bst2UI CCWGG 1 cut(s) 462
Bst4CI ACNGT 2 cut(s) 208, 673
BstAPI GCANNNNNTGC 1 cut(s) 444
BstC8I GCNNGC 1 cut(s) 743
BstDEI CTNAG 2 cut(s) 448, 706
BstF5I GGATG 3 cut(s) 150, 347, 617
BstKTI GATC 2 cut(s) 499, 579
BstMAI GTCTC 1 cut(s) 177
BstMBI GATC 2 cut(s) 496, 576
BstMWI GCNNNNNNNGC 4 cut(s) 435, 444, 509, 719
BstNI CCWGG 1 cut(s) 462
BstNSI RCATGY 1 cut(s) 768
BstSCI CCNGG 2 cut(s) 460, 492
BstSLI GKGCMC 1 cut(s) 747
BstV1I GCAGC 3 cut(s) 539, 709, 722
BsuRI GGCC 1 cut(s) 696
BtrI CACGTC 1 cut(s) 606
BtsCI GGATG 3 cut(s) 150, 347, 617
BtsIMutI CAGTG 4 cut(s) 351, 438, 585, 669
BveI ACCTGC 1 cut(s) 352
Cac8I GCNNGC 1 cut(s) 743
Cfr13I GGNCC 1 cut(s) 695
Csp6I GTAC 1 cut(s) 597
CspCI CAANNNNNGTGG 4 cut(s) 544, 579, 658, 693
CviAII CATG 3 cut(s) 310, 737, 765
CviQI GTAC 1 cut(s) 597
DdeI CTNAG 2 cut(s) 448, 706
DpnI GATC 2 cut(s) 498, 578
DpnII GATC 2 cut(s) 496, 576
EcoRII CCWGG 1 cut(s) 460
FaeI CATG 3 cut(s) 313, 740, 768
FaiI YATR 9 cut(s) 311, 319, 321, 420, 432, 477, 738, 766, 787
FaqI GGGAC 3 cut(s) 194, 616, 671
FatI CATG 3 cut(s) 309, 736, 764
FbaI TGATCA 1 cut(s) 576
Fnu4HI GCNGC 3 cut(s) 553, 711, 723
FokI GGATG 3 cut(s) 157, 334, 604
Fsp4HI GCNGC 3 cut(s) 553, 711, 723
FspBI CTAG 5 cut(s) 32, 306, 375, 582, 624
GluI GCNGC 3 cut(s) 553, 711, 723
HaeIII GGCC 1 cut(s) 696
HapII CCGG 1 cut(s) 493
Hin1II CATG 3 cut(s) 313, 740, 768
HincII GTYRAC 1 cut(s) 780
HindII GTYRAC 1 cut(s) 780
HinfI GANTC 2 cut(s) 278, 454
HpaI GTTAAC 1 cut(s) 780
HpaII CCGG 1 cut(s) 493
HphI GGTGA 2 cut(s) 97, 330
Hpy166II GTNNAC 3 cut(s) 256, 745, 780
Hpy188I TCNGA 2 cut(s) 451, 531
Hpy188III TCNNGA 1 cut(s) 643
Hpy8I GTNNAC 3 cut(s) 256, 745, 780
HpyAV CCTTC 3 cut(s) 3, 76, 699
HpyCH4III ACNGT 2 cut(s) 208, 673
HpyCH4IV ACGT 1 cut(s) 605
HpyCH4V TGCA 6 cut(s) 326, 347, 386, 555, 725, 745
HpyF10VI GCNNNNNNNGC 4 cut(s) 435, 444, 509, 719
HpyF3I CTNAG 2 cut(s) 448, 706
HpySE526I ACGT 1 cut(s) 605
Hsp92II CATG 3 cut(s) 313, 740, 768
Ksp22I TGATCA 1 cut(s) 576
KspAI GTTAAC 1 cut(s) 780
Kzo9I GATC 2 cut(s) 496, 576
LmnI GCTCC 2 cut(s) 41, 671
Lsp1109I GCAGC 3 cut(s) 539, 709, 722
LweI GCATC 2 cut(s) 356, 395
MaeI CTAG 5 cut(s) 32, 306, 375, 582, 624
MaeII ACGT 1 cut(s) 605
MaeIII GTNAC 2 cut(s) 85, 292
MalI GATC 2 cut(s) 498, 578
MboI GATC 2 cut(s) 496, 576
MboII GAAGA 2 cut(s) 58, 544
MfeI CAATTG 1 cut(s) 249
MhlI GDGCHC 1 cut(s) 747
MluCI AATT 7 cut(s) 27, 61, 149, 165, 249, 262, 748
MnlI CCTC 3 cut(s) 59, 715, 742
MseI TTAA 1 cut(s) 779
MspA1I CMGCKG 1 cut(s) 275
MspI CCGG 1 cut(s) 493
MspR9I CCNGG 2 cut(s) 462, 494
MunI CAATTG 1 cut(s) 249
Mva1269I GAATGC 1 cut(s) 512
MvaI CCWGG 1 cut(s) 462
MwoI GCNNNNNNNGC 4 cut(s) 435, 444, 509, 719
NciI CCSGG 1 cut(s) 494
NdeII GATC 2 cut(s) 496, 576
NlaIII CATG 3 cut(s) 313, 740, 768
NlaIV GGNNCC 2 cut(s) 37, 667
NmuCI GTSAC 2 cut(s) 85, 292
NspI RCATGY 1 cut(s) 768
PaqCI CACCTGC 1 cut(s) 352
PctI GAATGC 1 cut(s) 512
PfeI GAWTC 2 cut(s) 278, 454
PflMI CCANNNNNTGG 1 cut(s) 599
PfoI TCCNGGA 1 cut(s) 492
PkrI GCNGC 3 cut(s) 554, 712, 724
PsiI TTATAA 1 cut(s) 477
Psp6I CCWGG 1 cut(s) 460
PspGI CCWGG 1 cut(s) 460
PspN4I GGNNCC 2 cut(s) 37, 667
PspPI GGNCC 1 cut(s) 695
PvuII CAGCTG 1 cut(s) 275
RsaI GTAC 1 cut(s) 598
RsaNI GTAC 1 cut(s) 597
SaqAI TTAA 1 cut(s) 779
SatI GCNGC 3 cut(s) 553, 711, 723
Sau3AI GATC 2 cut(s) 496, 576
Sau96I GGNCC 1 cut(s) 695
ScaI AGTACT 1 cut(s) 598
ScrFI CCNGG 2 cut(s) 462, 494
SduI GDGCHC 1 cut(s) 747
SfaNI GCATC 2 cut(s) 356, 395
Sse9I AATT 7 cut(s) 27, 61, 149, 165, 249, 262, 748
SspMI CTAG 5 cut(s) 32, 306, 375, 582, 624
StyD4I CCNGG 2 cut(s) 460, 492
TaaI ACNGT 2 cut(s) 208, 673
TaiI ACGT 1 cut(s) 608
TasI AATT 7 cut(s) 27, 61, 149, 165, 249, 262, 748
TatI WGTACW 1 cut(s) 596
TfiI GAWTC 2 cut(s) 278, 454
Tru1I TTAA 1 cut(s) 779
Tru9I TTAA 1 cut(s) 779
TscAI CASTG 4 cut(s) 358, 445, 592, 676
TseFI GTSAC 2 cut(s) 85, 292
TseI GCWGC 3 cut(s) 552, 710, 722
Tsp45I GTSAC 2 cut(s) 85, 292
TspDTI ATGAA 4 cut(s) 161, 228, 624, 745
TspRI CASTG 4 cut(s) 358, 445, 592, 676
Van91I CCANNNNNTGG 1 cut(s) 599
VneI GTGCAC 1 cut(s) 743
XapI RAATTY 1 cut(s) 149
XceI RCATGY 1 cut(s) 768
XspI CTAG 5 cut(s) 32, 306, 375, 582, 624
ZrmI AGTACT 1 cut(s) 598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.