Rmu_co8483005.1_g000001

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8483005.1
OG0007207
Physical Location & Seq
Forward (+)
1 .. 667
667 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8483005.1_g000001.1.cds

Sequence Viewer

Length: 366 bp
caccagtcccctgtttccggcaaccaacacgagacatctccggtgacttcgccggataccgggacaggtgacgtctccaagactatgaccgggaacacgtggtgcgtggcgaatggtgaggcgggtaaagagaagctgcaggcggctctagatttcgcttgcggtgagggaggtgctgattgccatcagatccagcccggttccacgtgttacgatcctaacactctcgaggcccacgcctcgttcgcgttcaatagctattaccagaaaaaggcgcgcggagccggcacgtgctattttggcggggcggcctacgtagtctcgcagccacctaagtatgggaaatgtgagctgcccatgagctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_17 X8

Protein Analysis

121

Amino Acids

12.67

Weight (kDa)

5.71

Isoelectric Point (pI)

30.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 75
AccII CGCG 3 cut(s) 248, 277, 279
AciI CCGC 6 cut(s) 122, 143, 162, 279, 303, 308
AclWI GGATC 2 cut(s) 184, 209
AcvI CACGTG 3 cut(s) 99, 207, 291
AcyI GRCGYC 1 cut(s) 72
AdeI CACNNNGTG 1 cut(s) 102
AfiI CCNNNNNNNGG 4 cut(s) 17, 59, 271, 338
AflIII ACRYGT 2 cut(s) 96, 206
AgsI TTSAA 1 cut(s) 253
AluBI AGCT 4 cut(s) 136, 258, 352, 363
AluI AGCT 4 cut(s) 136, 258, 352, 363
Alw26I GTCTC 3 cut(s) 26, 79, 325
AlwI GGATC 2 cut(s) 184, 209
Ama87I CYCGRG 1 cut(s) 227
AoxI GGCC 2 cut(s) 231, 309
ApeKI GCWGC 3 cut(s) 136, 325, 352
AspLEI GCGC 2 cut(s) 277, 279
AspS9I GGNCC 1 cut(s) 232
AsuC2I CCSGG 3 cut(s) 61, 91, 198
AsuHPI GGTGA 4 cut(s) 55, 80, 128, 176
AvaI CYCGRG 1 cut(s) 227
BauI CACGAG 1 cut(s) 29
BbrPI CACGTG 3 cut(s) 99, 207, 291
BbvI GCAGC 3 cut(s) 123, 337, 339
BccI CCATC 1 cut(s) 192
BciVI GTATCC 1 cut(s) 49
BcnI CCSGG 3 cut(s) 61, 91, 198
BcoDI GTCTC 3 cut(s) 26, 79, 325
BfaI CTAG 1 cut(s) 149
BfmI CTRYAG 1 cut(s) 137
BfuI GTATCC 1 cut(s) 49
BisI GCNGC 5 cut(s) 137, 144, 309, 326, 353
BlsI GCNGC 5 cut(s) 138, 145, 310, 327, 354
Bme1390I CCNGG 3 cut(s) 61, 91, 198
BmeT110I CYCGRG 1 cut(s) 227
BmgT120I GGNCC 1 cut(s) 232
BmiI GGNNCC 2 cut(s) 202, 283
BmrFI CCNGG 3 cut(s) 61, 91, 198
BpuMI CCSGG 3 cut(s) 61, 91, 198
BsaAI YACGTR 4 cut(s) 99, 207, 291, 316
BsaBI GATNNNNATC 1 cut(s) 183
BsaHI GRCGYC 1 cut(s) 72
BsaWI WCCGGW 1 cut(s) 40
Bsc4I CCNNNNNNNGG 4 cut(s) 17, 59, 271, 338
Bse118I RCCGGY 1 cut(s) 284
Bse1I ACTGG 1 cut(s) 4
Bse8I GATNNNNATC 1 cut(s) 183
BseJI GATNNNNATC 1 cut(s) 183
BseLI CCNNNNNNNGG 4 cut(s) 17, 59, 271, 338
BseNI ACTGG 1 cut(s) 4
BsePI GCGCGC 1 cut(s) 275
BseXI GCAGC 3 cut(s) 123, 337, 339
Bsh1236I CGCG 3 cut(s) 248, 277, 279
BshFI GGCC 2 cut(s) 233, 311
BsiHKCI CYCGRG 1 cut(s) 227
BsiSI CCGG 7 cut(s) 18, 41, 53, 60, 90, 198, 285
BslFI GGGAC 1 cut(s) 76
BslI CCNNNNNNNGG 4 cut(s) 17, 59, 271, 338
BsmAI GTCTC 3 cut(s) 26, 79, 325
BsmBI CGTCTC 1 cut(s) 79
BsmFI GGGAC 1 cut(s) 76
BsnI GGCC 2 cut(s) 233, 311
BsoBI CYCGRG 1 cut(s) 227
Bsp143I GATC 2 cut(s) 189, 214
BspACI CCGC 6 cut(s) 122, 143, 162, 279, 303, 308
BspANI GGCC 2 cut(s) 233, 311
BspFNI CGCG 3 cut(s) 248, 277, 279
BspLI GGNNCC 2 cut(s) 202, 283
BspMAI CTGCAG 1 cut(s) 141
BspPI GGATC 2 cut(s) 184, 209
BsrFI RCCGGY 1 cut(s) 284
BsrI ACTGG 1 cut(s) 4
BssAI RCCGGY 1 cut(s) 284
BssHII GCGCGC 1 cut(s) 275
BssMI GATC 2 cut(s) 189, 214
BssNI GRCGYC 1 cut(s) 72
BssSI CACGAG 1 cut(s) 29
Bst2BI CACGAG 1 cut(s) 29
BstACI GRCGYC 1 cut(s) 72
BstBAI YACGTR 4 cut(s) 99, 207, 291, 316
BstC8I GCNNGC 4 cut(s) 141, 160, 277, 286
BstDEI CTNAG 1 cut(s) 333
BstFNI CGCG 3 cut(s) 248, 277, 279
BstHHI GCGC 2 cut(s) 277, 279
BstKTI GATC 2 cut(s) 192, 217
BstMAI GTCTC 3 cut(s) 26, 79, 325
BstMBI GATC 2 cut(s) 189, 214
BstMWI GCNNNNNNNGC 4 cut(s) 245, 281, 285, 300
BstSCI CCNGG 3 cut(s) 59, 89, 196
BstSFI CTRYAG 1 cut(s) 137
BstSNI TACGTA 1 cut(s) 316
BstUI CGCG 3 cut(s) 248, 277, 279
BstV1I GCAGC 3 cut(s) 123, 337, 339
BstX2I RGATCY 1 cut(s) 189
BstYI RGATCY 1 cut(s) 189
BsuI GTATCC 1 cut(s) 49
BsuRI GGCC 2 cut(s) 233, 311
Cac8I GCNNGC 4 cut(s) 141, 160, 277, 286
CfoI GCGC 2 cut(s) 277, 279
Cfr10I RCCGGY 1 cut(s) 284
Cfr13I GGNCC 1 cut(s) 232
CviAII CATG 1 cut(s) 358
DdeI CTNAG 1 cut(s) 333
DpnI GATC 2 cut(s) 191, 216
DpnII GATC 2 cut(s) 189, 214
DraIII CACNNNGTG 1 cut(s) 102
Eco105I TACGTA 1 cut(s) 316
Eco72I CACGTG 3 cut(s) 99, 207, 291
Eco88I CYCGRG 1 cut(s) 227
Esp3I CGTCTC 1 cut(s) 79
FaeI CATG 1 cut(s) 361
FaiI YATR 3 cut(s) 86, 339, 359
FaqI GGGAC 1 cut(s) 76
FatI CATG 1 cut(s) 357
FauI CCCGC 2 cut(s) 115, 296
Fnu4HI GCNGC 5 cut(s) 137, 144, 309, 326, 353
Fsp4HI GCNGC 5 cut(s) 137, 144, 309, 326, 353
FspBI CTAG 1 cut(s) 149
GlaI GCGC 2 cut(s) 276, 278
GluI GCNGC 5 cut(s) 137, 144, 309, 326, 353
HaeIII GGCC 2 cut(s) 233, 311
HapII CCGG 7 cut(s) 18, 41, 53, 60, 90, 198, 285
HhaI GCGC 2 cut(s) 277, 279
Hin1I GRCGYC 1 cut(s) 72
Hin1II CATG 1 cut(s) 361
Hin6I GCGC 2 cut(s) 275, 277
HinP1I GCGC 2 cut(s) 275, 277
HpaII CCGG 7 cut(s) 18, 41, 53, 60, 90, 198, 285
HphI GGTGA 4 cut(s) 55, 80, 128, 176
Hpy188I TCNGA 1 cut(s) 189
Hpy188III TCNNGA 2 cut(s) 149, 227
HpyCH4IV ACGT 5 cut(s) 72, 98, 206, 290, 315
HpyCH4V TGCA 1 cut(s) 139
HpyF10VI GCNNNNNNNGC 4 cut(s) 245, 281, 285, 300
HpyF3I CTNAG 1 cut(s) 333
HpySE526I ACGT 5 cut(s) 72, 98, 206, 290, 315
Hsp92I GRCGYC 1 cut(s) 72
Hsp92II CATG 1 cut(s) 361
HspAI GCGC 2 cut(s) 275, 277
KroI GCCGGC 1 cut(s) 284
KroNI GCCGGC 1 cut(s) 286
Kzo9I GATC 2 cut(s) 189, 214
LmnI GCTCC 1 cut(s) 281
Lsp1109I GCAGC 3 cut(s) 123, 337, 339
MaeI CTAG 1 cut(s) 149
MaeII ACGT 5 cut(s) 72, 98, 206, 290, 315
MaeIII GTNAC 3 cut(s) 43, 68, 209
MalI GATC 2 cut(s) 191, 216
MboI GATC 2 cut(s) 189, 214
MflI RGATCY 1 cut(s) 189
MnlI CCTC 5 cut(s) 112, 160, 164, 223, 250
MroNI GCCGGC 1 cut(s) 284
MspI CCGG 7 cut(s) 18, 41, 53, 60, 90, 198, 285
MspR9I CCNGG 3 cut(s) 61, 91, 198
MvnI CGCG 3 cut(s) 248, 277, 279
MwoI GCNNNNNNNGC 4 cut(s) 245, 281, 285, 300
NaeI GCCGGC 1 cut(s) 286
NciI CCSGG 3 cut(s) 61, 91, 198
NdeII GATC 2 cut(s) 189, 214
NgoMIV GCCGGC 1 cut(s) 284
NlaIII CATG 1 cut(s) 361
NlaIV GGNNCC 2 cut(s) 202, 283
NmuCI GTSAC 2 cut(s) 43, 68
PaeR7I CTCGAG 1 cut(s) 227
PauI GCGCGC 1 cut(s) 275
PdiI GCCGGC 1 cut(s) 286
PkrI GCNGC 5 cut(s) 138, 145, 310, 327, 354
PmaCI CACGTG 3 cut(s) 99, 207, 291
PmlI CACGTG 3 cut(s) 99, 207, 291
Ppu21I YACGTR 4 cut(s) 99, 207, 291, 316
PspCI CACGTG 3 cut(s) 99, 207, 291
PspN4I GGNNCC 2 cut(s) 202, 283
PspPI GGNCC 1 cut(s) 232
PstI CTGCAG 1 cut(s) 141
PsuI RGATCY 1 cut(s) 189
PteI GCGCGC 1 cut(s) 275
SatI GCNGC 5 cut(s) 137, 144, 309, 326, 353
Sau3AI GATC 2 cut(s) 189, 214
Sau96I GGNCC 1 cut(s) 232
ScrFI CCNGG 3 cut(s) 61, 91, 198
SfcI CTRYAG 1 cut(s) 137
Sfr274I CTCGAG 1 cut(s) 227
SlaI CTCGAG 1 cut(s) 227
SmlI CTYRAG 1 cut(s) 227
SmoI CTYRAG 1 cut(s) 227
SnaBI TACGTA 1 cut(s) 316
SsiI CCGC 6 cut(s) 122, 143, 162, 279, 303, 308
SspMI CTAG 1 cut(s) 149
StyD4I CCNGG 3 cut(s) 59, 89, 196
TaiI ACGT 5 cut(s) 75, 101, 209, 293, 318
TaqI TCGA 1 cut(s) 228
TauI GCSGC 2 cut(s) 146, 311
TseFI GTSAC 2 cut(s) 43, 68
TseI GCWGC 3 cut(s) 136, 325, 352
Tsp45I GTSAC 2 cut(s) 43, 68
XbaI TCTAGA 1 cut(s) 148
XhoI CTCGAG 1 cut(s) 227
XspI CTAG 1 cut(s) 149
ZraI GACGTC 1 cut(s) 73
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.