Rw2G040000

glucan endo-1,3-beta-glucosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0007207
Physical Location & Seq
Reverse (-)
65498332 .. 65500329
1998 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G040000.1

Sequence Viewer

Length: 693 bp
ATGATTGGTGGAAGGTTCAACTATGGTATAATAGTATTCCTGATCCTATTGGGTCATTTCTTCTGTTCAGAAACAGCTACATATTACTACTTCAATTTCCACAACCCAGAAAGACATCACAACCCCAATAACCACAGTTCCTACAATAACTCCCACCACTCCCACTTCATCAACACCAATTATGAACCCTACTTCCACCCAGATTCAACCTCTCCAATTACTACAACCCCCTTTTCTACTACCACAACATCATCCACTTCTGCAAGTTGGTGTGTTGCTAGCCCAAGTGCAACACAAATGGCATTACAAGTTGCCTTGGACTATGCTTGTGGCTATGGTGGCACTGATTGTTCAGAGATTCAGCTTGGTCGAAGCTGTTACAACCCAAATACTGTCCGAGACCATGCTTCTTTTGCATTCAATAACTTTTATCAGAAGAATCCAGTTCCAAATAGCTGCAATTTTGGTGGCACTGCTGTCATCACCAACAATGATCCAAGTACTGGGACATGTCAATATCCATCCACTAGCACAAGTTCATCGGTTTTGAACACAACAAATTCAAGTGGCTCAACGGTTTTCGGTGCGGTACCTTCTGGTCCTTCTACCTCTTCAGCAGCTGCTGCTGCAAGTTCGCACAGCCTGCGAAACTTGTTCACTATGATGTCTATGACGGTGGTTCTACTCGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
X8

Protein Analysis

230

Amino Acids

24.79

Weight (kDa)

6.38

Isoelectric Point (pI)

40.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 89 - 159 2.2e-20 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 589
AccB1I GGYRCC 1 cut(s) 589
AccB7I CCANNNNNTGG 1 cut(s) 503
AciI CCGC 1 cut(s) 587
AclWI GGATC 2 cut(s) 37, 488
AcsI RAATTY 1 cut(s) 559
AcuI CTGAAG 1 cut(s) 597
AfaI GTAC 2 cut(s) 502, 591
AfiI CCNNNNNNNGG 1 cut(s) 503
AflIII ACRYGT 1 cut(s) 509
AgsI TTSAA 6 cut(s) 19, 94, 207, 421, 550, 564
AloI GAACNNNNNNTCC 2 cut(s) 177, 209
AluBI AGCT 5 cut(s) 77, 364, 375, 456, 620
AluI AGCT 5 cut(s) 77, 364, 375, 456, 620
Alw26I GTCTC 1 cut(s) 393
AlwI GGATC 2 cut(s) 37, 488
AlwNI CAGNNNCTG 2 cut(s) 620, 623
ApeKI GCWGC 5 cut(s) 456, 617, 620, 623, 626
ApoI RAATTY 1 cut(s) 559
Asp718I GGTACC 1 cut(s) 589
AspS9I GGNCC 1 cut(s) 599
AsuHPI GGTGA 1 cut(s) 475
AsuNHI GCTAGC 1 cut(s) 278
AvaII GGWCC 1 cut(s) 599
BanI GGYRCC 1 cut(s) 589
BbvI GCAGC 5 cut(s) 443, 607, 610, 613, 629
BccI CCATC 1 cut(s) 529
BcoDI GTCTC 1 cut(s) 393
BfaI CTAG 2 cut(s) 279, 528
BisI GCNGC 5 cut(s) 457, 618, 621, 624, 627
BlsI GCNGC 5 cut(s) 458, 619, 622, 625, 628
BmcAI AGTACT 1 cut(s) 502
Bme18I GGWCC 1 cut(s) 599
BmgT120I GGNCC 1 cut(s) 599
BmiI GGNNCC 1 cut(s) 591
BmrI ACTGGG 1 cut(s) 513
BmtI GCTAGC 1 cut(s) 282
BmuI ACTGGG 1 cut(s) 513
BsaI GGTCTC 1 cut(s) 393
BsaJI CCNNGG 1 cut(s) 315
BsaXI ACNNNNNCTCC 2 cut(s) 134, 164
Bsc4I CCNNNNNNNGG 1 cut(s) 503
Bse1I ACTGG 2 cut(s) 443, 508
BseDI CCNNGG 1 cut(s) 315
BseGI GGATG 2 cut(s) 251, 521
BseLI CCNNNNNNNGG 1 cut(s) 503
BseNI ACTGG 2 cut(s) 443, 508
BseXI GCAGC 5 cut(s) 443, 607, 610, 613, 629
BshNI GGYRCC 1 cut(s) 589
BslFI GGGAC 1 cut(s) 520
BslI CCNNNNNNNGG 1 cut(s) 503
BsmAI GTCTC 1 cut(s) 393
BsmFI GGGAC 1 cut(s) 520
BsmI GAATGC 1 cut(s) 416
Bso31I GGTCTC 1 cut(s) 393
Bsp143I GATC 2 cut(s) 42, 493
BspACI CCGC 1 cut(s) 587
BspLI GGNNCC 1 cut(s) 591
BspOI GCTAGC 1 cut(s) 282
BspPI GGATC 2 cut(s) 37, 488
BspT107I GGYRCC 1 cut(s) 589
BspTNI GGTCTC 1 cut(s) 393
BsrI ACTGG 2 cut(s) 443, 508
BssECI CCNNGG 1 cut(s) 315
BssMI GATC 2 cut(s) 42, 493
BssT1I CCWWGG 1 cut(s) 315
Bst4CI ACNGT 4 cut(s) 137, 394, 577, 676
Bst6I CTCTTC 1 cut(s) 616
BstAPI GCANNNNNTGC 2 cut(s) 623, 643
BstC8I GCNNGC 2 cut(s) 280, 644
BstF5I GGATG 2 cut(s) 251, 521
BstKTI GATC 2 cut(s) 45, 496
BstMAI GTCTC 1 cut(s) 393
BstMBI GATC 2 cut(s) 42, 493
BstMWI GCNNNNNNNGC 5 cut(s) 339, 413, 623, 626, 643
BstNSI RCATGY 1 cut(s) 513
BstV1I GCAGC 5 cut(s) 443, 607, 610, 613, 629
BtsCI GGATG 2 cut(s) 251, 521
BtsI GCAGTG 1 cut(s) 471
BtsIMutI CAGTG 2 cut(s) 342, 471
Cac8I GCNNGC 2 cut(s) 280, 644
CaiI CAGNNNCTG 2 cut(s) 620, 623
Cfr13I GGNCC 1 cut(s) 599
Csp6I GTAC 2 cut(s) 501, 590
CspCI CAANNNNNGTGG 2 cut(s) 448, 483
CviAII CATG 2 cut(s) 404, 510
CviJI RGCY 9 cut(s) 77, 282, 333, 364, 375, 456, 570, 620, 642
CviKI_1 RGCY 9 cut(s) 77, 282, 333, 364, 375, 456, 570, 620, 642
CviQI GTAC 2 cut(s) 501, 590
DpnI GATC 2 cut(s) 44, 495
DpnII GATC 2 cut(s) 42, 493
Eam1104I CTCTTC 1 cut(s) 616
EarI CTCTTC 1 cut(s) 616
Eco130I CCWWGG 1 cut(s) 315
Eco31I GGTCTC 1 cut(s) 393
Eco47I GGWCC 1 cut(s) 599
Eco57I CTGAAG 1 cut(s) 597
EcoT14I CCWWGG 1 cut(s) 315
ErhI CCWWGG 1 cut(s) 315
FaeI CATG 2 cut(s) 407, 513
FaqI GGGAC 1 cut(s) 520
FatI CATG 2 cut(s) 403, 509
Fnu4HI GCNGC 5 cut(s) 457, 618, 621, 624, 627
FokI GGATG 2 cut(s) 238, 508
Fsp4HI GCNGC 5 cut(s) 457, 618, 621, 624, 627
FspBI CTAG 2 cut(s) 279, 528
GluI GCNGC 5 cut(s) 457, 618, 621, 624, 627
Hin1II CATG 2 cut(s) 407, 513
HinfI GANTC 3 cut(s) 203, 358, 439
HphI GGTGA 1 cut(s) 475
Hpy166II GTNNAC 1 cut(s) 657
Hpy188I TCNGA 4 cut(s) 70, 355, 398, 435
Hpy188III TCNNGA 1 cut(s) 40
Hpy8I GTNNAC 1 cut(s) 657
HpyAV CCTTC 3 cut(s) 6, 603, 612
HpyCH4III ACNGT 4 cut(s) 137, 394, 577, 676
HpyCH4V TGCA 5 cut(s) 263, 290, 416, 459, 629
HpyF10VI GCNNNNNNNGC 5 cut(s) 339, 413, 623, 626, 643
Hsp92II CATG 2 cut(s) 407, 513
KpnI GGTACC 1 cut(s) 593
Kzo9I GATC 2 cut(s) 42, 493
LpnPI CCDG 7 cut(s) 53, 120, 213, 456, 489, 582, 656
Lsp1109I GCAGC 5 cut(s) 443, 607, 610, 613, 629
MaeI CTAG 2 cut(s) 279, 528
MaeIII GTNAC 1 cut(s) 377
MalI GATC 2 cut(s) 44, 495
MboI GATC 2 cut(s) 42, 493
MboII GAAGA 3 cut(s) 52, 448, 603
MluCI AATT 5 cut(s) 94, 178, 216, 460, 559
MnlI CCTC 2 cut(s) 220, 619
MseI TTAA 1 cut(s) 691
MslI CAYNNNNRTG 1 cut(s) 662
MspA1I CMGCKG 1 cut(s) 620
Mva1269I GAATGC 1 cut(s) 416
MwoI GCNNNNNNNGC 5 cut(s) 339, 413, 623, 626, 643
NdeII GATC 2 cut(s) 42, 493
NheI GCTAGC 1 cut(s) 278
NlaIII CATG 2 cut(s) 407, 513
NlaIV GGNNCC 1 cut(s) 591
NspI RCATGY 1 cut(s) 513
PciI ACATGT 1 cut(s) 509
PctI GAATGC 1 cut(s) 416
PfeI GAWTC 3 cut(s) 203, 358, 439
PflMI CCANNNNNTGG 1 cut(s) 503
PkrI GCNGC 5 cut(s) 458, 619, 622, 625, 628
PscI ACATGT 1 cut(s) 509
PspN4I GGNNCC 1 cut(s) 591
PspPI GGNCC 1 cut(s) 599
PstNI CAGNNNCTG 2 cut(s) 620, 623
PvuII CAGCTG 1 cut(s) 620
RsaI GTAC 2 cut(s) 502, 591
RsaNI GTAC 2 cut(s) 501, 590
RseI CAYNNNNRTG 1 cut(s) 662
SaqAI TTAA 1 cut(s) 691
SatI GCNGC 5 cut(s) 457, 618, 621, 624, 627
Sau3AI GATC 2 cut(s) 42, 493
Sau96I GGNCC 1 cut(s) 599
ScaI AGTACT 1 cut(s) 502
SetI ASST 9 cut(s) 17, 79, 212, 366, 377, 458, 595, 611, 622
SinI GGWCC 1 cut(s) 599
SmiMI CAYNNNNRTG 1 cut(s) 662
Sse9I AATT 5 cut(s) 94, 178, 216, 460, 559
SsiI CCGC 1 cut(s) 587
SspMI CTAG 2 cut(s) 279, 528
StyI CCWWGG 1 cut(s) 315
TaaI ACNGT 4 cut(s) 137, 394, 577, 676
TaqI TCGA 1 cut(s) 370
TasI AATT 5 cut(s) 94, 178, 216, 460, 559
TatI WGTACW 1 cut(s) 500
TfiI GAWTC 3 cut(s) 203, 358, 439
Tru1I TTAA 1 cut(s) 691
Tru9I TTAA 1 cut(s) 691
TscAI CASTG 2 cut(s) 349, 478
TseI GCWGC 5 cut(s) 456, 617, 620, 623, 626
TspDTI ATGAA 3 cut(s) 157, 198, 528
TspRI CASTG 2 cut(s) 349, 478
Van91I CCANNNNNTGG 1 cut(s) 503
VpaK11BI GGWCC 1 cut(s) 599
XapI RAATTY 1 cut(s) 559
XceI RCATGY 1 cut(s) 513
XspI CTAG 2 cut(s) 279, 528
ZrmI AGTACT 1 cut(s) 502
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.