FvH4_6g38310

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0015328
Physical Location & Seq
Forward (+)
30287042 .. 30288627
1586 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g38310.t1

Sequence Viewer

Length: 1578 bp
ATGCTAGAAAACCAATGCACCAACATGAAAGACCTCCAGAAAATCCATGCCCACCTTATCAAAACTGGCCTAGCCAATGACACTGTTGCCGCCAGCCGTGTCTTGGCCTTTTGTGCCTCCCCAGCCGGAGACATCAACTATGCCTACATGGTTTTCAGACATATCCACAACCCAAACCTCTTCATCTGGAACACCATCATTAGAGGTTTCTCTAACAGCTCAAACCCTGAAGCTGCCATCTCTCTTTTCATCGACATGTTGGTCACTTCAACTGTCCAGCCACAAAGGTTGACTTACCCTTCAGTTTTCAAGGCTTATGCTCAACTCGGGCTTGCCCATGATGGAGCTCAGCTTCATGGGAGAGTTGTGAAGTTGGGTCTTGAAAGTGACCAGTTTGTAAGGAACACCATCATACATATGTACTCAAACTGTGGCCTTTTGAGTGAAGCAAGAAGAGTGTTTGATGAAGACTTGGAGTTTGACATTGTGGCTTGGAATTCTATGATAATGGGCCTTTCTAAATGTGGTGAAGTCGGTGAATCTAGAAGGCTGTTTGATAAAATGCCTCAAAGGAATTCGATTTCATGGAATTCGATGATTGGTGGATCTGTTAGGAATGGAATGTACACAGAAGCATTGGATCTTTTTGGTGAAATGCAGAAGCAGAAGATTAAGCCTAGTGAGTTTACAATGGTGAGCCTGTTGAATGCTTCAGCTCAATTAGGAGCAATTAGACAAGGGGAGTGGATTCATGAATATATAAGGAAAAATCATATTCAGCTCAATCCCATTGTTGTAACAGCTATCATTAATATGTACTCCAAATGTGGAAGCATTGAAAAAGCTGTACATGTGTTTGAGGCAGCTCCTAGAACAGGGTTATCTTGTTGGAACTCCATCATTATGGGGTTAGCCACTAATGGTTGTGAAGAAGAAGCAATTGAGCTATTCTCAAGGCTCAAATCTTCGAGTTTTGTACCTGATGATGTCAGTTTTCTTGGTGTTCTAACCGCATGCAGTCACTCGGGGATGGTGGAGAAAGCAAGGAAGTACTTTTCAGTAATGAGAGAAACATATAGGATTGCACCATCCATTAAGCACTATAGTTGCATGGTGGATGTGTTAGGCAGAGCTGGACTTCTTGAAGAAGCTGAGAAGCTCATAGATGGTATGCCATTAAAAGCAGACGCGATCATTTGGGGTTCGTTGCTCTCATCTTGTAGGAAGCACAGAGACATTGAGATGGCTAAGCGGGCTGCAAAGCATGTGATTGAGTTGGATCCAAGTGATTGTTGTGGTTATGTGTTAATGTCAAATGTTTATGCTGCCTCTAGTCAATTTGAGGAAGCAATGCGTGAAAGGCTTTCAATGAAAGGGCAGAAGATTGAGAAAGAACCGGGATGTAGTTTGATCGAAGTAGATGGAGAGGTTCATGAGTTTATAGCTGGTGGAAGGCTGCATCAGAAAGCCCCAGAGATTTATTCACTACTGAATGGATTGGGGTTTATGTTGGAAAGAAATGCAACAGATTATGCTGAACTTGCTGCCTATCAGATTATGGAAAAATCAAATTATTAG

Protein Analysis

526

Amino Acids

58.66

Weight (kDa)

6.37

Isoelectric Point (pI)

42.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 58 - 107 1.5e-06 PPR repeat family
PPR PF01535 163 - 192 1.8e-06 PPR repeat
PPR_2 PF13041 192 - 237 9.9e-12 PPR repeat family
PPR PF01535 194 - 222 7e-08 PPR repeat
PPR_2 PF13041 296 - 340 1e-05 PPR repeat family
E_motif PF20431 410 - 472 9.8e-15 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015328)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42920 AT2G42920
fragaria_vesca FvH4_6g38310
malus_domestica MD09G1151200.v1.1 MD17G1138800.v1.1
prunus_persica Prupe.3G173600_v2.0.a1
pyrus_communis pycom17g13190
rosa_chinensis RchiOBHm_Chr2g0151611
rosa_laevigata RLG00000020563
rosa_multiflora Rmu_sc0005287.1_g000006
rosa_roxburghii Rroxscaffold_2G00096320
rosa_rugosa Rorug02G0427200
rosa_samantha Rh2AG488100 Rh2BG500700 Rh2CG474600
rosa_wichuraiana Rw2G040010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 260
AccII CGCG 1 cut(s) 1190
AciI CCGC 3 cut(s) 90, 1011, 1252
AclWI GGATC 4 cut(s) 613, 648, 1274, 1287
AcsI RAATTY 3 cut(s) 496, 574, 589
AcuI CTGAAG 3 cut(s) 249, 285, 696
AfaI GTAC 6 cut(s) 422, 626, 818, 849, 978, 1052
AfiI CCNNNNNNNGG 2 cut(s) 103, 875
AflIII ACRYGT 2 cut(s) 255, 850
AgsI TTSAA 7 cut(s) 270, 310, 383, 706, 839, 1145, 1368
Alw21I GWGCWC 1 cut(s) 349
Alw26I GTCTC 2 cut(s) 123, 1227
AlwI GGATC 4 cut(s) 613, 648, 1274, 1287
Ama87I CYCGRG 2 cut(s) 326, 1024
AoxI GGCC 4 cut(s) 67, 105, 433, 511
ApeKI GCWGC 6 cut(s) 233, 863, 1256, 1325, 1456, 1544
ApoI RAATTY 3 cut(s) 496, 574, 589
ArsI GACNNNNNNTTYG 2 cut(s) 461, 493
AseI ATTAAT 1 cut(s) 810
AspS9I GGNCC 1 cut(s) 511
AsuC2I CCSGG 1 cut(s) 1398
AsuHPI GGTGA 4 cut(s) 539, 548, 662, 706
AvaI CYCGRG 2 cut(s) 326, 1024
BamHI GGATCC 1 cut(s) 1279
BanII GRGCYC 1 cut(s) 349
BbsI GAAGAC 1 cut(s) 474
Bbv12I GWGCWC 1 cut(s) 349
BbvI GCAGC 6 cut(s) 220, 875, 1243, 1312, 1443, 1531
BceAI ACGGC 1 cut(s) 81
BcnI CCSGG 1 cut(s) 1398
BcoDI GTCTC 2 cut(s) 123, 1227
BfaI CTAG 6 cut(s) 5, 71, 543, 678, 870, 1332
BfmI CTRYAG 1 cut(s) 1102
BisI GCNGC 7 cut(s) 90, 234, 864, 1257, 1326, 1457, 1545
BlpI GCTNAGC 2 cut(s) 348, 1248
BlsI GCNGC 7 cut(s) 91, 235, 865, 1258, 1327, 1458, 1546
BmcAI AGTACT 1 cut(s) 1052
Bme1390I CCNGG 1 cut(s) 1398
BmeT110I CYCGRG 2 cut(s) 326, 1024
BmgT120I GGNCC 1 cut(s) 511
BmiI GGNNCC 1 cut(s) 1281
BmrFI CCNGG 1 cut(s) 1398
BmsI GCATC 1 cut(s) 1468
BpiI GAAGAC 1 cut(s) 474
BplI GAGNNNNNCTC 2 cut(s) 935, 967
BpmI CTGGAG 1 cut(s) 20
Bpu1102I GCTNAGC 2 cut(s) 348, 1248
BpuEI CTTGAG 1 cut(s) 937
BpuMI CCSGG 1 cut(s) 1398
Bsc4I CCNNNNNNNGG 2 cut(s) 103, 875
Bse1I ACTGG 2 cut(s) 70, 391
Bse3DI GCAATG 1 cut(s) 1356
BseGI GGATG 4 cut(s) 1035, 1088, 1123, 1406
BseLI CCNNNNNNNGG 2 cut(s) 103, 875
BseMI GCAATG 1 cut(s) 1356
BseMII CTCAG 2 cut(s) 362, 1143
BseNI ACTGG 2 cut(s) 70, 391
BseXI GCAGC 6 cut(s) 220, 875, 1243, 1312, 1443, 1531
BseYI CCCAGC 1 cut(s) 121
Bsh1236I CGCG 1 cut(s) 1190
BshFI GGCC 4 cut(s) 69, 107, 435, 513
BsiHKAI GWGCWC 1 cut(s) 349
BsiHKCI CYCGRG 2 cut(s) 326, 1024
BsiSI CCGG 2 cut(s) 126, 1397
BslI CCNNNNNNNGG 2 cut(s) 103, 875
BsmAI GTCTC 2 cut(s) 123, 1227
BsmI GAATGC 1 cut(s) 712
BsnI GGCC 4 cut(s) 69, 107, 435, 513
BsoBI CYCGRG 2 cut(s) 326, 1024
Bsp1286I GDGCHC 1 cut(s) 349
Bsp1407I TGTACA 2 cut(s) 624, 847
Bsp143I GATC 5 cut(s) 605, 640, 1191, 1279, 1410
Bsp1720I GCTNAGC 2 cut(s) 348, 1248
BspACI CCGC 3 cut(s) 90, 1011, 1252
BspANI GGCC 4 cut(s) 69, 107, 435, 513
BspCNI CTCAG 2 cut(s) 361, 1144
BspFNI CGCG 1 cut(s) 1190
BspHI TCATGA 2 cut(s) 751, 1432
BspLI GGNNCC 1 cut(s) 1281
BspPI GGATC 4 cut(s) 613, 648, 1274, 1287
BsrDI GCAATG 1 cut(s) 1356
BsrGI TGTACA 2 cut(s) 624, 847
BsrI ACTGG 2 cut(s) 70, 391
BssMI GATC 5 cut(s) 605, 640, 1191, 1279, 1410
Bst4CI ACNGT 3 cut(s) 85, 274, 431
Bst6I CTCTTC 2 cut(s) 185, 448
BstAUI TGTACA 2 cut(s) 624, 847
BstC8I GCNNGC 4 cut(s) 94, 333, 1015, 1254
BstDEI CTNAG 3 cut(s) 348, 1152, 1248
BstENI CCTNNNNNAGG 1 cut(s) 873
BstF5I GGATG 4 cut(s) 1035, 1088, 1123, 1406
BstFNI CGCG 1 cut(s) 1190
BstKTI GATC 5 cut(s) 608, 643, 1194, 1282, 1413
BstMAI GTCTC 2 cut(s) 123, 1227
BstMBI GATC 5 cut(s) 605, 640, 1191, 1279, 1410
BstMWI GCNNNNNNNGC 5 cut(s) 113, 122, 1253, 1360, 1541
BstNSI RCATGY 4 cut(s) 259, 854, 1017, 1268
BstSCI CCNGG 1 cut(s) 1396
BstSFI CTRYAG 1 cut(s) 1102
BstUI CGCG 1 cut(s) 1190
BstV1I GCAGC 6 cut(s) 220, 875, 1243, 1312, 1443, 1531
BstV2I GAAGAC 1 cut(s) 474
BstX2I RGATCY 3 cut(s) 605, 640, 1279
BstXI CCANNNNNNTGG 1 cut(s) 904
BstYI RGATCY 3 cut(s) 605, 640, 1279
BsuRI GGCC 4 cut(s) 69, 107, 435, 513
BtsCI GGATG 4 cut(s) 1035, 1088, 1123, 1406
BtsIMutI CAGTG 1 cut(s) 81
Cac8I GCNNGC 4 cut(s) 94, 333, 1015, 1254
CciI TCATGA 2 cut(s) 751, 1432
Cfr13I GGNCC 1 cut(s) 511
CseI GACGC 1 cut(s) 1196
Csp6I GTAC 6 cut(s) 421, 625, 817, 848, 977, 1051
CspCI CAANNNNNGTGG 4 cut(s) 270, 305, 725, 760
CviQI GTAC 6 cut(s) 421, 625, 817, 848, 977, 1051
DdeI CTNAG 3 cut(s) 348, 1152, 1248
DpnI GATC 5 cut(s) 607, 642, 1193, 1281, 1412
DpnII GATC 5 cut(s) 605, 640, 1191, 1279, 1410
DrdI GACNNNNNNGTC 1 cut(s) 260
DseDI GACNNNNNNGTC 1 cut(s) 260
Eam1104I CTCTTC 2 cut(s) 185, 448
EarI CTCTTC 2 cut(s) 185, 448
Ecl136II GAGCTC 1 cut(s) 347
Eco24I GRGCYC 1 cut(s) 349
Eco53kI GAGCTC 1 cut(s) 347
Eco57I CTGAAG 3 cut(s) 249, 285, 696
Eco88I CYCGRG 2 cut(s) 326, 1024
EcoICRI GAGCTC 1 cut(s) 347
EcoNI CCTNNNNNAGG 1 cut(s) 873
EcoRI GAATTC 3 cut(s) 496, 574, 589
EcoT38I GRGCYC 1 cut(s) 349
FalI AAGNNNNNCTT 2 cut(s) 277, 309
FauI CCCGC 1 cut(s) 1245
FauNDI CATATG 1 cut(s) 417
Fnu4HI GCNGC 7 cut(s) 90, 234, 864, 1257, 1326, 1457, 1545
FokI GGATG 4 cut(s) 1042, 1075, 1130, 1413
FriOI GRGCYC 1 cut(s) 349
Fsp4HI GCNGC 7 cut(s) 90, 234, 864, 1257, 1326, 1457, 1545
FspBI CTAG 6 cut(s) 5, 71, 543, 678, 870, 1332
GluI GCNGC 7 cut(s) 90, 234, 864, 1257, 1326, 1457, 1545
GsaI CCCAGC 1 cut(s) 125
GsuI CTGGAG 1 cut(s) 20
HaeIII GGCC 4 cut(s) 69, 107, 435, 513
HapII CCGG 2 cut(s) 126, 1397
HgaI GACGC 1 cut(s) 1196
HincII GTYRAC 1 cut(s) 291
HindII GTYRAC 1 cut(s) 291
HinfI GANTC 2 cut(s) 539, 748
HpaII CCGG 2 cut(s) 126, 1397
HphI GGTGA 4 cut(s) 539, 548, 662, 706
Hpy166II GTNNAC 3 cut(s) 291, 627, 687
Hpy188I TCNGA 3 cut(s) 158, 1464, 1554
Hpy188III TCNNGA 7 cut(s) 37, 187, 380, 543, 752, 1142, 1433
Hpy8I GTNNAC 3 cut(s) 291, 627, 687
HpyAV CCTTC 3 cut(s) 309, 540, 1446
HpyCH4III ACNGT 3 cut(s) 85, 274, 431
HpyCH4V TGCA 8 cut(s) 18, 658, 1017, 1085, 1110, 1259, 1459, 1523
HpyF10VI GCNNNNNNNGC 5 cut(s) 113, 122, 1253, 1360, 1541
HpyF3I CTNAG 3 cut(s) 348, 1152, 1248
Kzo9I GATC 5 cut(s) 605, 640, 1191, 1279, 1410
LmnI GCTCC 3 cut(s) 344, 725, 871
Lsp1109I GCAGC 6 cut(s) 220, 875, 1243, 1312, 1443, 1531
LweI GCATC 1 cut(s) 1468
MaeI CTAG 6 cut(s) 5, 71, 543, 678, 870, 1332
MaeIII GTNAC 4 cut(s) 262, 386, 796, 1019
MalI GATC 5 cut(s) 607, 642, 1193, 1281, 1412
MboI GATC 5 cut(s) 605, 640, 1191, 1279, 1410
MboII GAAGA 9 cut(s) 172, 465, 479, 679, 941, 944, 957, 1157, 1393
MfeI CAATTG 1 cut(s) 939
MflI RGATCY 3 cut(s) 605, 640, 1279
MhlI GDGCHC 1 cut(s) 349
MluCI AATT 8 cut(s) 496, 574, 589, 719, 729, 939, 1337, 1570
MmeI TCCRAC 3 cut(s) 869, 1257, 1491
MnlI CCTC 9 cut(s) 44, 127, 188, 197, 576, 853, 1336, 1339, 1420
MseI TTAA 5 cut(s) 672, 810, 1095, 1178, 1307
MslI CAYNNNNRTG 6 cut(s) 23, 254, 416, 812, 902, 1241
MspI CCGG 2 cut(s) 126, 1397
MspR9I CCNGG 1 cut(s) 1398
MunI CAATTG 1 cut(s) 939
Mva1269I GAATGC 1 cut(s) 712
MvnI CGCG 1 cut(s) 1190
MwoI GCNNNNNNNGC 5 cut(s) 113, 122, 1253, 1360, 1541
NciI CCSGG 1 cut(s) 1398
NdeI CATATG 1 cut(s) 417
NdeII GATC 5 cut(s) 605, 640, 1191, 1279, 1410
NlaIV GGNNCC 1 cut(s) 1281
NmuCI GTSAC 3 cut(s) 262, 386, 1019
NspI RCATGY 4 cut(s) 259, 854, 1017, 1268
PaeI GCATGC 1 cut(s) 1017
PagI TCATGA 2 cut(s) 751, 1432
PciI ACATGT 2 cut(s) 255, 850
PctI GAATGC 1 cut(s) 712
PfeI GAWTC 2 cut(s) 539, 748
PkrI GCNGC 7 cut(s) 91, 235, 865, 1258, 1327, 1458, 1546
PscI ACATGT 2 cut(s) 255, 850
PshBI ATTAAT 1 cut(s) 810
Psp124BI GAGCTC 1 cut(s) 349
PspFI CCCAGC 1 cut(s) 121
PspN4I GGNNCC 1 cut(s) 1281
PspPI GGNCC 1 cut(s) 511
PsuI RGATCY 3 cut(s) 605, 640, 1279
RsaI GTAC 6 cut(s) 422, 626, 818, 849, 978, 1052
RsaNI GTAC 6 cut(s) 421, 625, 817, 848, 977, 1051
RseI CAYNNNNRTG 6 cut(s) 23, 254, 416, 812, 902, 1241
SacI GAGCTC 1 cut(s) 349
SaqAI TTAA 5 cut(s) 672, 810, 1095, 1178, 1307
SatI GCNGC 7 cut(s) 90, 234, 864, 1257, 1326, 1457, 1545
Sau3AI GATC 5 cut(s) 605, 640, 1191, 1279, 1410
Sau96I GGNCC 1 cut(s) 511
ScaI AGTACT 1 cut(s) 1052
ScrFI CCNGG 1 cut(s) 1398
SduI GDGCHC 1 cut(s) 349
SfaNI GCATC 1 cut(s) 1468
SfcI CTRYAG 1 cut(s) 1102
SmiMI CAYNNNNRTG 6 cut(s) 23, 254, 416, 812, 902, 1241
SmlI CTYRAG 1 cut(s) 952
SmoI CTYRAG 1 cut(s) 952
SphI GCATGC 1 cut(s) 1017
Sse9I AATT 8 cut(s) 496, 574, 589, 719, 729, 939, 1337, 1570
SsiI CCGC 3 cut(s) 90, 1011, 1252
SspMI CTAG 6 cut(s) 5, 71, 543, 678, 870, 1332
SstI GAGCTC 1 cut(s) 349
StyD4I CCNGG 1 cut(s) 1396
TaaI ACNGT 3 cut(s) 85, 274, 431
TaqI TCGA 5 cut(s) 252, 578, 593, 968, 1413
TasI AATT 8 cut(s) 496, 574, 589, 719, 729, 939, 1337, 1570
TatI WGTACW 5 cut(s) 420, 624, 816, 847, 1050
TauI GCSGC 1 cut(s) 92
TfiI GAWTC 2 cut(s) 539, 748
Tru1I TTAA 5 cut(s) 672, 810, 1095, 1178, 1307
Tru9I TTAA 5 cut(s) 672, 810, 1095, 1178, 1307
TscAI CASTG 1 cut(s) 88
TseFI GTSAC 3 cut(s) 262, 386, 1019
TseI GCWGC 6 cut(s) 233, 863, 1256, 1325, 1456, 1544
Tsp45I GTSAC 3 cut(s) 262, 386, 1019
TspRI CASTG 1 cut(s) 88
VspI ATTAAT 1 cut(s) 810
XagI CCTNNNNNAGG 1 cut(s) 873
XapI RAATTY 3 cut(s) 496, 574, 589
XbaI TCTAGA 1 cut(s) 542
XceI RCATGY 4 cut(s) 259, 854, 1017, 1268
XcmI CCANNNNNNNNNTGG 1 cut(s) 100
XspI CTAG 6 cut(s) 5, 71, 543, 678, 870, 1332
ZrmI AGTACT 1 cut(s) 1052
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.