MD17G1138800.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0015328
Physical Location & Seq
Reverse (-)
12450989 .. 12451570
582 bp
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UTR
Exon/CDS
Intron
MD17G1138800.v1.1.491

Sequence Viewer

Length: 582 bp
ATGACACCATGTTGTTGCTCTTTCACTTCTCCAACCTCCATATCCAAATTCATCTCAGAAAGTCCACACCTCTCCATGCTAGAAAACCAGTGCACCTCCATGAAAGACCTTCAAAAGATCCACGCCCACCTCCTCAAAACCGGCCTAGCCAATGACACCGTCGCCGCCAGCCGCATATTGGCCTTTTCTGCCTCCCCAGCTGGAGACATCAACTACGCCTGTATGGTTTTCAGACACATCAAAGAACCAAACCCCTTCATTTGGAACACCATCATTAGAGGCTTCTCCGAAAGCCAAAACCCAAAAACTGCAATCTCTCTTTTCACCGAATTAGATCCAATTGACAGCTGCGGTTATGTGCTTGTGTCGAATATCTATGCTGCTTCCAGCCAATTTCAGGAAGCAATAAAGGAAAGGCTTTCGATGAAGGAGCAGAAGATAGAGAAAGAACCAGGATGTAGTTTGATCAAAGTGGACGGAGAGGTTCATGAGTTTATAGCCGGTGGAAGACTGCATCAAAAAGCGCCAGAGATCTATTCTTTGCTGGATGAGTTGGGGTTTATGATGCCGGAAATGGAGTAG

Protein Analysis

194

Amino Acids

21.45

Weight (kDa)

5.53

Isoelectric Point (pI)

51.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
E_motif PF20431 100 - 156 8.8e-13 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015328)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42920 AT2G42920
fragaria_vesca FvH4_6g38310
malus_domestica MD09G1151200.v1.1 MD17G1138800.v1.1
prunus_persica Prupe.3G173600_v2.0.a1
pyrus_communis pycom17g13190
rosa_chinensis RchiOBHm_Chr2g0151611
rosa_laevigata RLG00000020563
rosa_multiflora Rmu_sc0005287.1_g000006
rosa_roxburghii Rroxscaffold_2G00096320
rosa_rugosa Rorug02G0427200
rosa_samantha Rh2AG488100 Rh2BG500700 Rh2CG474600
rosa_wichuraiana Rw2G040010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 165, 172, 351
AclWI GGATC 2 cut(s) 112, 329
AcsI RAATTY 1 cut(s) 47
AfiI CCNNNNNNNGG 3 cut(s) 178, 261, 397
AgsI TTSAA 1 cut(s) 113
AjnI CCWGG 1 cut(s) 451
AluBI AGCT 2 cut(s) 200, 348
AluI AGCT 2 cut(s) 200, 348
Alw21I GWGCWC 1 cut(s) 95
Alw26I GTCTC 1 cut(s) 198
Alw44I GTGCAC 1 cut(s) 91
AlwI GGATC 2 cut(s) 112, 329
AoxI GGCC 2 cut(s) 142, 180
ApaLI GTGCAC 1 cut(s) 91
ApeKI GCWGC 2 cut(s) 348, 380
ApoI RAATTY 1 cut(s) 47
AspLEI GCGC 1 cut(s) 526
AsuHPI GGTGA 1 cut(s) 316
BaeGI GKGCMC 1 cut(s) 95
BbsI GAAGAC 1 cut(s) 514
Bbv12I GWGCWC 1 cut(s) 95
BbvI GCAGC 2 cut(s) 335, 367
BccI CCATC 1 cut(s) 278
BciT130I CCWGG 1 cut(s) 453
BclI TGATCA 1 cut(s) 465
BcoDI GTCTC 1 cut(s) 198
BfaI CTAG 2 cut(s) 80, 146
BfoI RGCGCY 1 cut(s) 527
BglII AGATCT 1 cut(s) 531
BisI GCNGC 4 cut(s) 165, 172, 349, 381
BlsI GCNGC 4 cut(s) 166, 173, 350, 382
Bme1390I CCNGG 1 cut(s) 453
BmrFI CCNGG 1 cut(s) 453
BmsI GCATC 2 cut(s) 523, 555
BpiI GAAGAC 1 cut(s) 514
BpmI CTGGAG 1 cut(s) 222
Bsc4I CCNNNNNNNGG 3 cut(s) 178, 261, 397
Bse118I RCCGGY 2 cut(s) 140, 500
Bse1I ACTGG 1 cut(s) 88
BseBI CCWGG 1 cut(s) 453
BseGI GGATG 2 cut(s) 461, 553
BseLI CCNNNNNNNGG 3 cut(s) 178, 261, 397
BseMII CTCAG 1 cut(s) 69
BseNI ACTGG 1 cut(s) 88
BseRI GAGGAG 1 cut(s) 122
BseSI GKGCMC 1 cut(s) 95
BseXI GCAGC 2 cut(s) 335, 367
BseYI CCCAGC 1 cut(s) 196
BshFI GGCC 2 cut(s) 144, 182
BsiHKAI GWGCWC 1 cut(s) 95
BsiSI CCGG 3 cut(s) 141, 501, 569
BslI CCNNNNNNNGG 3 cut(s) 178, 261, 397
BsmAI GTCTC 1 cut(s) 198
BsnI GGCC 2 cut(s) 144, 182
Bsp1286I GDGCHC 1 cut(s) 95
Bsp143I GATC 4 cut(s) 117, 334, 465, 531
BspACI CCGC 3 cut(s) 165, 172, 351
BspANI GGCC 2 cut(s) 144, 182
BspCNI CTCAG 1 cut(s) 68
BspHI TCATGA 1 cut(s) 487
BspPI GGATC 2 cut(s) 112, 329
BsrFI RCCGGY 2 cut(s) 140, 500
BsrI ACTGG 1 cut(s) 88
BssAI RCCGGY 2 cut(s) 140, 500
BssMI GATC 4 cut(s) 117, 334, 465, 531
Bst2UI CCWGG 1 cut(s) 453
Bst4CI ACNGT 1 cut(s) 160
BstC8I GCNNGC 1 cut(s) 169
BstDEI CTNAG 1 cut(s) 55
BstF5I GGATG 2 cut(s) 461, 553
BstH2I RGCGCY 1 cut(s) 527
BstHHI GCGC 1 cut(s) 526
BstKTI GATC 4 cut(s) 120, 337, 468, 534
BstMAI GTCTC 1 cut(s) 198
BstMBI GATC 4 cut(s) 117, 334, 465, 531
BstMWI GCNNNNNNNGC 2 cut(s) 188, 197
BstNI CCWGG 1 cut(s) 453
BstSCI CCNGG 1 cut(s) 451
BstSLI GKGCMC 1 cut(s) 95
BstV1I GCAGC 2 cut(s) 335, 367
BstV2I GAAGAC 1 cut(s) 514
BstX2I RGATCY 3 cut(s) 117, 334, 531
BstYI RGATCY 3 cut(s) 117, 334, 531
BsuRI GGCC 2 cut(s) 144, 182
BtsCI GGATG 2 cut(s) 461, 553
BtsIMutI CAGTG 1 cut(s) 95
Cac8I GCNNGC 1 cut(s) 169
CciI TCATGA 1 cut(s) 487
CfoI GCGC 1 cut(s) 526
Cfr10I RCCGGY 2 cut(s) 140, 500
CviAII CATG 4 cut(s) 9, 76, 100, 488
DdeI CTNAG 1 cut(s) 55
DpnI GATC 4 cut(s) 119, 336, 467, 533
DpnII GATC 4 cut(s) 117, 334, 465, 531
EcoRII CCWGG 1 cut(s) 451
FaeI CATG 4 cut(s) 12, 79, 103, 491
FatI CATG 4 cut(s) 8, 75, 99, 487
FbaI TGATCA 1 cut(s) 465
Fnu4HI GCNGC 4 cut(s) 165, 172, 349, 381
FokI GGATG 2 cut(s) 468, 560
Fsp4HI GCNGC 4 cut(s) 165, 172, 349, 381
FspBI CTAG 2 cut(s) 80, 146
GlaI GCGC 1 cut(s) 525
GluI GCNGC 4 cut(s) 165, 172, 349, 381
GsaI CCCAGC 1 cut(s) 200
GsuI CTGGAG 1 cut(s) 222
HaeII RGCGCY 1 cut(s) 527
HaeIII GGCC 2 cut(s) 144, 182
HapII CCGG 3 cut(s) 141, 501, 569
HhaI GCGC 1 cut(s) 526
Hin1II CATG 4 cut(s) 12, 79, 103, 491
Hin6I GCGC 1 cut(s) 524
HinP1I GCGC 1 cut(s) 524
HpaII CCGG 3 cut(s) 141, 501, 569
HphI GGTGA 1 cut(s) 316
Hpy166II GTNNAC 3 cut(s) 65, 93, 475
Hpy188I TCNGA 3 cut(s) 58, 233, 289
Hpy188III TCNNGA 2 cut(s) 398, 488
Hpy8I GTNNAC 3 cut(s) 65, 93, 475
Hpy99I CGWCG 1 cut(s) 164
HpyAV CCTTC 3 cut(s) 119, 265, 421
HpyCH4III ACNGT 1 cut(s) 160
HpyCH4V TGCA 3 cut(s) 93, 311, 514
HpyF10VI GCNNNNNNNGC 2 cut(s) 188, 197
HpyF3I CTNAG 1 cut(s) 55
Hsp92II CATG 4 cut(s) 12, 79, 103, 491
HspAI GCGC 1 cut(s) 524
Ksp22I TGATCA 1 cut(s) 465
Kzo9I GATC 4 cut(s) 117, 334, 465, 531
LmnI GCTCC 1 cut(s) 430
Lsp1109I GCAGC 2 cut(s) 335, 367
LweI GCATC 2 cut(s) 523, 555
MaeI CTAG 2 cut(s) 80, 146
MalI GATC 4 cut(s) 119, 336, 467, 533
MboI GATC 4 cut(s) 117, 334, 465, 531
MboII GAAGA 2 cut(s) 448, 519
MfeI CAATTG 1 cut(s) 339
MflI RGATCY 3 cut(s) 117, 334, 531
MhlI GDGCHC 1 cut(s) 95
MluCI AATT 4 cut(s) 47, 329, 339, 392
MmeI TCCRAC 1 cut(s) 56
MnlI CCTC 8 cut(s) 46, 80, 106, 140, 143, 202, 272, 475
MslI CAYNNNNRTG 1 cut(s) 98
MspA1I CMGCKG 2 cut(s) 200, 348
MspI CCGG 3 cut(s) 141, 501, 569
MspR9I CCNGG 1 cut(s) 453
MunI CAATTG 1 cut(s) 339
MvaI CCWGG 1 cut(s) 453
MwoI GCNNNNNNNGC 2 cut(s) 188, 197
NdeII GATC 4 cut(s) 117, 334, 465, 531
NlaIII CATG 4 cut(s) 12, 79, 103, 491
PagI TCATGA 1 cut(s) 487
PflFI GACNNNGTC 1 cut(s) 158
PkrI GCNGC 4 cut(s) 166, 173, 350, 382
Psp6I CCWGG 1 cut(s) 451
PspFI CCCAGC 1 cut(s) 196
PspGI CCWGG 1 cut(s) 451
PsuI RGATCY 3 cut(s) 117, 334, 531
PsyI GACNNNGTC 1 cut(s) 158
PvuII CAGCTG 2 cut(s) 200, 348
RseI CAYNNNNRTG 1 cut(s) 98
SatI GCNGC 4 cut(s) 165, 172, 349, 381
Sau3AI GATC 4 cut(s) 117, 334, 465, 531
ScrFI CCNGG 1 cut(s) 453
SduI GDGCHC 1 cut(s) 95
SetI ASST 8 cut(s) 38, 72, 98, 111, 132, 202, 350, 486
SfaNI GCATC 2 cut(s) 523, 555
SmiMI CAYNNNNRTG 1 cut(s) 98
Sse9I AATT 4 cut(s) 47, 329, 339, 392
SsiI CCGC 3 cut(s) 165, 172, 351
SspMI CTAG 2 cut(s) 80, 146
StyD4I CCNGG 1 cut(s) 451
TaaI ACNGT 1 cut(s) 160
TaqI TCGA 2 cut(s) 368, 422
TasI AATT 4 cut(s) 47, 329, 339, 392
TauI GCSGC 2 cut(s) 167, 174
TscAI CASTG 1 cut(s) 95
TseI GCWGC 2 cut(s) 348, 380
TspDTI ATGAA 5 cut(s) 40, 116, 247, 440, 476
TspGWI ACGGA 1 cut(s) 492
TspRI CASTG 1 cut(s) 95
Tth111I GACNNNGTC 1 cut(s) 158
VneI GTGCAC 1 cut(s) 91
XapI RAATTY 1 cut(s) 47
XcmI CCANNNNNNNNNTGG 1 cut(s) 175
XspI CTAG 2 cut(s) 80, 146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.